FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841071
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA191652.2786423500513036No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT188412.2401200374284693No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA155771.8520434065613962No Hit
CCTATCTCATCGTCTTATATTGGCTTCACTGTCTTTCAACAGGCCTTGCT137661.6367227023640099No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC136251.61995836261148No Hit
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA127621.5173510916438684No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA127521.5161621313777316No Hit
CCAAATAACAAGTGTTGGAAGCAGGGAAGCTCTTCATCCTCACTAGATAA126291.501537920104248No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG120531.4330538087747646No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG119441.4200941418738728No Hit
GCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATTGAACTT117651.3988117531100228No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT113411.3483998378258197No Hit
ACCTCCATCCAAAACTTCTCGAAAAGAAACTACCTCAGGGACAAGTACTG100581.1958562356804598No Hit
GGTCCAGCAGCCCTTTCCTGAGCAAGCGGTCTCTGAGTCGGAGTCCACTC100341.1930027310417313No Hit
CGACACATGACTGCAATGAAATTCAGTCCTGGAAGGAAAAGAAGAAGTAA99931.1881279939505702No Hit
TTACCCAGTACCATCAATGCTGTTAACAGTTCTTCATCCTTTTTCCAGCT97991.165062164787515No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCCTGAAAACCAGAT95091.130582317069546No Hit
TGTTTCAGGAAGGAATGTTCCCAATAGTAGACATAAAAGTCTTCGCACAG92641.1014527905491927No Hit
CTCAGTCACACATGCCAGAGATTGCTCAGGAAGCAATGGAGGTAAGGGGA88641.0538943799037181No Hit
TTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTA85221.0132319388018372No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACACTCGGTAGCA81270.9662680082894309No Hit
GAAGAGACCAAGCAAGTTTTGAGACTCAGTCTGACAGAGTTCTCATTACC80830.9610365831184288No Hit
ATATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTG78560.9340471850771218No Hit
CAGCAGTTACCAGGGCACAGGGTCAGTGCAGTTTCCAGGGGACCAGGACC78160.9292913440125745No Hit
GGAAATTGAAGAAATCTGCCTAGTAGATGAGAACCAGTTCACCTTAACCA78110.928696863879506No Hit
GGTCTCTCATGGCACTGTACTCTTCTTGTCCAGCTGTATCCAGTATGTCC75600.8988539611994707No Hit
TTTTTGTCCAGCCAGCAAATGAGAGTCTGTACCCGACCATTTCACAAAAG74820.889580071123603No Hit
GTCCTCCAGAGCACAAACCTGTGGCAGATACACACTGGTCCAGCCTTAAC74240.8826841015800092No Hit
ACGCCTTCTGTCTGAGCACCCAGAATTAGAACATATCATCTGGACCCTTT73470.8735291075307554No Hit
AATTTGCATCTGTTTGTCCACATTAGGCTTAGGTTACCACAAGGATCTCC72100.8572403518846803No Hit
CCAGTGCAACAGGTGGCTTGGGATCAATAAAAGCTGAGGTGATGGCAGAT72010.8561702876451572No Hit
TCCCTTAGAGCTTCCACACATGGACTGGTCATTAATATCATTCACTCTCT71920.8551002234056341No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA71830.8540301591661109No Hit
TCTGGGGTATCAGGTAGGTGTCCAGCTCCTGGCACTGGTAGAGTGCTACA71440.849393214128177No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC70430.8373847154401947No Hit
GCCAAACGAAAATTATGGCAGGTTGTTACGAGGCATTGGATGATTCAGAG70300.8358390670942168No Hit
TTAAATTTCACTCCACTTACCTACCAAGGGCCTGGTTTAAGGCCCTGTAT69570.8271596571514177No Hit
TGACACACTTGCAGAAACAGTATTGGCTGATCGGTTTGAGAGATTGGTGG65080.7737753412018724No Hit
GGGATCGATGAAGGACCGGATATCGGGAAGTTCAAAGCGTTCGAATGAGG63580.7559409372098194No Hit
TTTTGTGTTTGCACAGTGCCTTTCAGAATGTCCCACCCATAGAGTAGCAG60020.7136139517353469No Hit
CTAATGAGCGGACGGCAAAGAACGCTTTTTCAGACGTGGGGCTCAAGTAT58970.7011298689409098No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC58180.6917370828384286No Hit
TATGTCATGATTCATCTTACTAGCCTCAAACATATCTTCTTTGCCAGGAC58050.6901914344924507No Hit
ATTTCTCTTCTGACCTCTCAATTTTACCTTTTCTAGATGTATTCTTGGGG57930.6887646821730865No Hit
CTAACAGTACTCGGCCTGCTCGCTGGTATACCAAACTTGGATTCTTTCCT57140.6793718960706052No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC54850.652144705976071No Hit
ATTACAACCCATCTGAGTGGAGCCCTGGCTTCTCAGGCAGACCTGGTGTC54460.6475077609381372No Hit
GTAACTTGATTTGCTGTTGTATTTGCTTAGGAAGAGCAGCACTTCAGAAA53100.6313379013186758No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG52670.6262253721742873No Hit
TTAACTACAATTCCCAGCAAGCCGCGGAGCCAACCTGGGCCTCTGCCGGC52580.6251553079347641No Hit
TGTGATTATTTCTATTTTAGCAACCAAAGGACACAATGAGATTAGATGAA51310.6100555125548259No Hit
CCAGTGTTGTGTTTCCCAAAGTCTTTCCTGTTGTGTAAGTATCTCCTTTT47340.5628537899891923No Hit
CTCACGTAATTTTGTACTTTTTCTTCCTATTGGTCTTTGTTTTTCTCTAG47110.5601191813770776No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC46650.554649964152848No Hit
ATCTGGTGTTGAAAATTCTAATGACTTTGCATTTTTGAAGGTTTTTCCTT46580.5538176919665522No Hit
CCTCTCTCCTTCTTCCCCAAGTACACACACAAACCTGCCAATGCCAGTGG45750.5439493217576162No Hit
GCCCTCTCAGAAACATCTGCAGGAGGACTTGGGGAAACAAATTCATATGA45470.540620233012433No Hit
GGATACAGTAGCCAGAATGACGACTCAATCAGTGCTTCGGTTGTTGCTTT43490.517078819742923No Hit
TGAACTTATATATTTGACACAACCCAGCAGTTCGTAAGTAGTTCACAGAA42940.5105395382791702No Hit
CTACTCTTTAGCTTCCTACCTAAGAATAAAAATGGGATTGTTTGCACTAA41710.49591532700568686No Hit
AACTGAGCGACCTCAGAGCCCTGGCAGCTCCAAGGCGCCTTTGCCAGCAG41600.49460747071293626No Hit
GCTGCCCCAAAGTGTAAAGAAATGCAGAATTCTCTCAATAATGATAAAAA40910.4864036448765919No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG40460.4810533236789759No Hit
CGATGCGCGGGAAGACGTTCCGCTTTGAAATGCAGCGGGATTTGGTGAGT40460.4810533236789759No Hit
GTTAGAAGGCTGGCTCCCATGCTGTTCTAACACAGCTTCTAGTTCAGCCA39510.4697582011506758No Hit
TCCCCTTTGCTTTGTCTTTTTCCAGCCTCCCCCACTGGCAGGATTGCCAT38870.4621488554473998No Hit
TGGGTCCAGTCAGTGAACGTAAGGGTTCTATGATTTCAGTGATGTCTTCA37970.45144821305216803No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT37440.4451467236416426No Hit
TTCAACGTTGCCTCCTTTGCCCCTCCCACCCTTATTACCTGGAGATGATG37120.4413420507900046No Hit
CCAGACTCTCCAGAACCAAAGGCAATCACACCACCTCAGCAACCATATAA36690.4362295216456162No Hit
TATTTCTGGACAGTCTACGAAAAGCTCTTGCTGGCCATGGAGGAAGTAGG36140.4296902401818634No Hit
CCACTGTAAGTTTATGTCATGGTTGTTTTTTATATTTCAGTCGCCCTTAC35950.4274312156762033No Hit
TTTGCTAATAGATGCCTAAGCCCAGAAAGGGTGCTTCTTCAACTAAAATA35900.4268367355431349No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT35640.42374543885117905No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT35590.42315095871811065No Hit
ACAATAGTGGCCAAAAATGCTCATTGATAATAACAGTTTACATTTGTTTT34720.4128070044027199No Hit
CCAAGGAACATCTTCAGTATCTCTAGGATTCTCTGAGCATGGCAGTTTCT33520.39853948120907745No Hit
CCAGAGACCAGACTCCAGAGCTGGGAGGCGAGGTCACATTCCACTGGCCC32690.38867111100014146No Hit
AGGTTCAACTACGCGTGTAAATTCTACTGCAAATGCAGAGACACAAGCAA32000.3804672851637971No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC31930.3796350129775013No Hit
GGAGTTGAACCCAGCCGTGACAGCCGCCTTGCTGCAACTTTTATCCCAGC31590.375592548072636No Hit
TTTTGTGCGAAGGAAACCGATGGGGATCGGAACCGTAGCGGTTGAGCTGC30370.3610872328257662No Hit
GTGCAGGCTGATTTTCTTTTTCCTGTGTATCTTCTACCAGGTGCTTGGGC30360.3609683367991525No Hit
AGAGGAACGTTACACACCAGCCATAGATGTTTGGAGCTGTGGGTAAGGTT27810.33064985001266245No Hit
AGTCTTCAGAATGCCAGAGATATACAGGATATGCGAATTAAGAAGAAACA27430.3261318010013423No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC27300.3245861526553644No Hit
GGTGGTGTACCTGGTGAAAGACTAATGAATTCATCGGACATGTTACTGTT26610.31638232681902No Hit
GCTTTCAAGTGATAATTGCCTTCATTTTAGGCTTGGGAAGATACACATGC26070.30996194138188093No Hit
TCTCCATATCTCTCTCAATTTCAACAAGACAAACAACAGTTGGTATTAGG25450.3025903877318324No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC25150.2990235069334218No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA24840.29533773010839753No Hit
ATTTACCCTACCAATTGCCCAGTGCGGGACTACCAGCTGCACATTTCCCG24780.2946243539487154No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG24300.2889173446712584No Hit
CCATCATGTGAGTCATCAGAACCTAACAGTTCATCACTTCTGGAAAACCA24120.2867772161922121No Hit
TTGTGGCACCAAATACGAAACACCCATAAAGAAAAAAGAACTGAATTCTC23970.2849937757930068No Hit
TGTCATAATGAATGCCCCATCGATTGGTCAGGTAGACAGCAGCAAGCAAT23930.284518191686552No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAATTCTTTAACTTACTAAAA23830.28332923142041516No Hit
GGCAGTTCTCTTGAGTCAGCTGATGAAAACCCAAGAGCCAGCAGGCAGTC23370.2778600141961856No Hit
CACGTTTTTCCCATCTAGCAATTCCATGCACTTCTCATTTCTGTTGAAGT23330.27738443008973085No Hit
CTGTGGTGCCACCTAAGCTCTTAAGTGATAATTTATGTAGACAAACTGAA23320.27726553406311716No Hit
TCGGGGGCCTACAAGAGTCCCACCAAGAGGGGGAAGAGGGAGAGGAGTTC22300.2651381393485211No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT22030.2619279466299516No Hit
GACCTTTTCCTCTGCCCTTATCATCGCTTTTCAGTGATGGAGGAAATGTT21920.260620090337201No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCAGAGTATTGCCAATCATGTTC21560.2563398333791083No Hit
ATATATGTCTTCCACCCTTGACTCTCAGGATAGTGCAGCAGGATGCAGCG21540.25610204132588094No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG21380.25419970490006194No Hit
GCCTACCAGTCCAGCACCCGGTCACCGAGCCCCTACAGTAGGCGACAGAG20950.24908717575567343No Hit
TATTTAGTAGCCAGGACAGTAGAAGGACTGAAGAGTGAGAGGAGCTCCCA20100.23898101349351003No Hit
GCAACAGCTTCATTGCTGAGGGACTAGACATCACTCGCCTGTAACTTGAA20020.23802984528060056No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA19130.22744809891198245No Hit
GAAGTTCATCCTGTTTTAAGTCACACTTGTGATTTGTTAAATTTTTTAAC19070.22673472275230033No Hit
GCAGGAAGGAAAAGGAACCTGTATTCTTGTAGGTGGTCATGAAATCACTT18450.21936316910225176No Hit
GGGATAATGGGGTTTTCCTGTGTCAGAATCTCAATGGAACCCAGCATCCT18190.2162718724102959No Hit
TATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTGA17850.2122294075054306No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT17210.20462006180215464No Hit
GAAAAACAATTTATATCTGTCAGTGAATCCACTAGGACTGCTCCCACCAG16480.19594065185935552No Hit
TTTGCAGACCTTCTCAGGAGTCACTACTTCATTCCTGCTCTTAGTGTCTG16280.1935627313270818No Hit
TGAAGAAGGAGATGGTGTGGAATTGATGGAAGCCAAATCACAGTTATTTC16030.1905903306617396No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT15470.18393215317137318No Hit
CCATTTTCTTCACACCTGTCGTGAAGGAAACCAGCATGCAGCTGAACTTC15450.18369436111814577No Hit
CTGTATGTAGTCGGTGCTGTGACTTGTTTGTGCTCATCTCTGTTCTGTAG15270.18155423263909945No Hit
TCAATGCAGAGGTTGAAGATGGTATGTTGCCAACACGAGCTGACTCTGGG15220.180959752506031No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA14830.17632280746809723No Hit
GCCACAAAGTAAAAATGTTGTGTGTTTACTTTTTTGCATCTTGGCAGGCT14770.1756094313084151No Hit
TCTATGCTTGTTTCCCGACTGTGTGTGGCAGAGCAAAGGGCCCCAGACTG14400.1712102783237087No Hit
GGTATCAGATGCTTCATTACAAAACGCAAGACAAGTGTTTTCTGAAATAG14400.1712102783237087No Hit
GAATCAGGGATCACAACACCCCCCAAAATGAGGAGAGTAGCAGAAACTGA14250.16942683792450342No Hit
AGGACATGGCCGCGCACAGGCCGGTGGAATGGGTCCAGGCCGTGGTCAGC13930.16562216507286542No Hit
ACTCACTCCATTGTTCTTGCTTTTGCTTTGTTTTCAGCCGACTTTGTGGT13330.15848840347604423No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA13040.15504041870424734No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCGATATATGT12890.153256978305042No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA12410.14754996902758508No Hit
AAACGTGATGAGACCCGAGTTATGATCTTCTCTTCATTTCGAGATAGTGT11760.13982172729769543No Hit
TCAAAGGGCTCCACTGGTTTTTCTGAGCAGGACTTCACTTTTTCAAGCTT11590.13780049484526274No Hit
ATATTTAGTGAATGTGATTGATGGTACTTTAATTTTGTCACTTTGTGTTT11000.13078562927505527No Hit
CCCAAGATTACTGGCATCTTCCTGGGGCTGGCTCACCTGTCGGGAAGATT10770.12805102066294047No Hit
ATTTGTTGCAGGTTTTGGTTTTAATGGCTTGTGGCGGTTTGCAGGACCGT10640.12650537231696254No Hit
ATCCATGAAGTCCAGAAGTAGAAGTCCTGCATATTCAAGACATTCATCTT10550.12543530807743936No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTACTG10470.12448413986452986No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACGCGATATATGT10460.12436524383791618No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG10390.12353297165162036No Hit
TCAATGGCAAGTAAGTTATTTATGGTCGTTTTTAAGGATAAGCTGTTAAC10380.12341407562500668No Hit
AAAGCAGAAGCCTTAGAAACTCTGCAAATTATCAGAAGAGAATGTCTCAC9960.11842044250723185No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT9950.11830154648061815No Hit
CTTAAATGTTGAAGGTGGTTCTTCAGAAAATAATCACTCTATTAAAGTTT9580.11390239349591176No Hit
TGTTAATAAAAATAAAACTTAACAATTTTCCCCTTTTTTTACCCCCAGTG9470.1125945372031612No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCAATATATGT9290.11045440872411484No Hit
GGGATAAAAGCACAAAGGTCTGCTGATTTCTCACCAGCATTCTTATCTAC9270.11021661667088747No Hit
GCTTCCAGTACTTCAACTTTGCCCCCTTCTACTCACTCAAAGACATCTGC9270.11021661667088747No Hit
GGGAAAATTATGCTTACTAATGTGGTTTTAATTTCATCATGTTTCATATA9260.11009772064427378No Hit
GCCACTACCACAGCTCCTTCTCTGAGTGGTAAAGGCAATCCTGAGGAAGA9170.1090276564047506No Hit
GGAAACACTGATGGGCCTGAAACAGGGTTCAGTGCCATTGACACTGATGA8910.10593635971279476No Hit
TTCCCAATCACAGCCATTCGTGAAATCAAAATCCTTCGTCAGTTAATCCA8550.10165610275470205No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTACT8550.10165610275470205No Hit
GTCTTACTTAGCATGTAGCTAGGAAGTTTTTAGATGATGAAGCAGAACTT8530.10141831070147468No Hit
AAGTCCTCCATTTCTGTATCCATGCGTTTAGGACTCAGTTCCTCTGGAAA8450.10046714248856518No Hit
ATCTTATGTGGGATGATATTGCTATTTTAGCACGCTACATGCTGATGCTG8420.10011045440872413No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)