FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841071
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG182182.1660478128481424No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT174012.0689097591047605No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT155341.8469308774170075No Hit
CAGCTGTGCCATTTGAGGGATGCTCAGGTCGGTTTGGCTCTGCAGCAGGT134501.5991515579540847No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG126531.5043914247429764No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC124891.484892476378332No Hit
GTAGTCAGTGCATTCTACAACAGCGCGCATGTTAGCAAGTTCATCAACCA124821.484060204192036No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT121741.4474402279950207No Hit
AGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGG120881.4372151697062434No Hit
CAACTGTCAGTCTGCCATTCTTTAAGACAGCTAAGAGGGGAGGATCTAAC116001.3791939087187646No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACCTCCATGATCTCCAACAAAG114941.3665909298977137No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA107601.2793212463632677No Hit
GGATCCGTGGCGAGTTCGTCCACTGTTACAGTGGCCAGTGGTAGGGGAGA99361.1813509204335901No Hit
GCACAAAGGCCCCATTCTGGCACTGACCTATTGCATCCCCAGCCCCAGAC98111.1664889171068793No Hit
GTGTACACAGTGTCCACCAAGGTCCTGAGATCCTCATTTCTCTTCCTTGT97931.1643487886278328No Hit
CAATGATGAACACTGCGGGCTTCCAGACAATGTTAACACGCTGAATGCCA97141.1549560025253516No Hit
GCTGTCTACCTGACCAATCGATGGGGCATTCATTATGACATGAAGATCAG93321.1095377203589234No Hit
CAGAAATGACATCTACCTGTTACTCACAGAAACCACACACCAAAGGAACA86281.025834917622888No Hit
GGTTGCTGAGGTGGTGTGATTGCCTTTGGTTCTGGAGAGTCTGGGGGAGA85241.0134697308550644No Hit
AGCTTCATCATACCTTTCACTAAGACAAGAATTTAGAAGTGGACAGGAAA84261.0018179202469233No Hit
TAGGAGCCAGGAGAGAATGACCTGTCCCGGTAACTGGAACGGAAGGCAAT76860.9138348605527952No Hit
CTGTGACAGTTCCCAGCGGGTCCGGATATGAATGATAGACTGGACAATGG75310.8954059764276737No Hit
AGTGAGGATGAAGAGCTTCCCTGCTTCCAACACTTGTTATTTGGTAAAGT74870.8901745512566716No Hit
TGCAATTTGGTCTCTGCATGTACCACAGAATTGCTGCTTCCCTCAGCCTT74260.8829218936332367No Hit
GGAAGGTGACGTGAAAGATCACTGTGCAGCAGCAATCTTGACTTCTGGAA73350.8721023552113911No Hit
CTGCCCCCTTACCCTCCACACCCCCAGGATTCTTACAGAAAACAAGTGGT70570.8390492598127862No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG70110.8335800425885567No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTGCTTGATTTTCTTACTTGGTCCAA69740.8291808896038503No Hit
AACAGATATGCTATAGTACAGAAGGCAAAAGAAAAGTGATTACCTTGCTT69620.827754137284486No Hit
GTTGTAAAATTTCTTCACTCTGAATGTCAGCAAAAACCTTATGTGAATGC69190.8226416081400977No Hit
TAATCTATATTGATCAGGTGAAGTAAAATGGAGAAAGGAACTGGTAAACA68450.8138433021706847No Hit
TGGTGGCAAACTCTCCTTCTCAACCAAAAAGAAAGCAATCAACTATTTCA68360.8127732379311615No Hit
GGTTGAACTAAAACGACAATACAATGACCACCATTCAAAATTGAGAGGTC67970.8081362928932279No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC67760.8056394763343404No Hit
CCTGGAGTCGATTGATTAGAGCCTAGTCCAGGAGAATGAATTGACACTAA67590.8036182438819077No Hit
TTATTTTTTGGGGGGAAATTTTTTAGGATCTGATTCTTCTGAAGATACCG65940.7840003994906494No Hit
TCCACCAATCTCTCAAACCGATCAGCCAATACTGTTTCTGCAAGTGTGTC65280.7761532617341461No Hit
GGCACTTTTCTTCCCCGCTTACTCTAATCACTTACCCACTGAGAACAAGG62940.7483315915065435No Hit
GGTCTTTGTGCCCAGACTTCAGCTCCCGTTCTTTCCTGGTCTTCTCCTTG62180.7392954934839033No Hit
AAAGAGAGTGAGTCGTTGCGAAGCCCTGTGTTTCAGCTCATTGTGATTAA58490.6954228596634529No Hit
CACTCAAGAGCACATGTACAGCTGTACCTGATCAGTTCTAGCTGAGCCAG58100.6907859146255192No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA57750.6866245536940401No Hit
CGACAAGCAACACATCATCGTCCGACTCCAGCTGAGCCTCCGCTGCTGCT57260.6807986483899695No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC55030.6542848344551173No Hit
TCTAAAGTATGCAGGTTTTGTTCAAGAAGTGCGGTACCTGCTGAATACAA54590.6490534092841151No Hit
ACCTGTATAGGGTATGCTCTTTGAATAATTACATCAACACAACCAACATT53930.6412062715276118No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT49740.5913888363764772No Hit
AAGAGGCTTCCAAATTGAGGGTGGGCGCCTGGGAGGGGTGTTTCTGTTGC49670.5905565641901813No Hit
AGGGCCTATTAGCCATATCTCCCAGCTCTCAACAGGAGGTGGAGAGGCAG49480.5882975396845213No Hit
GCAGAATTCCGAAGTTCAGCTGCATGCTGGTTTCCTTCACGACAGGTGTG49190.5848495549127244No Hit
ACTTTCAAGTTTCCCCACCCTTTACACAGTTTCCCCGCGGAAAACGCCGC48320.5745056005973337No Hit
CACAACCTTGCACTGCTTTATGAAGTGTTTCACCAAACATATTTCGAAGT46520.55310431580687No Hit
GTAGACGATGTAAAGCAAGCACATTGCCGTCACTTATGAAGGAAAGACTA46300.5504886032213689No Hit
ATCACAATATCTCATTTTGCCCTCTTTGCAACCAGTGCACATTACTGGGT46230.5496563310350732No Hit
GCAGTCTGGGGCCCTTTGGCAGGCCCTGGTTGCCCTGAGGCGGGTGCTGC44110.5244503733929716No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG43530.5175544038493778No Hit
TATGAAGTAGCCTGCTATAATCGATCAAACTAACCCTAACTATCAAGATG41970.4990066236976427No Hit
TGTGGTTTAGCCCTCGAAGTAAGAAAGTCAGATATGTTGTGAGTAAAGCT41960.49888772767102896No Hit
CACTGGGCAATTGGTAGGGTAAATCCACAGGGCGCCCGCGGAGGTGCAGA41110.4887815654088656No Hit
CCTCAGCTTGCCATCAGGAGTCGTTACCTTTGCTAAGCTCGGAGGGTTTC41030.4878303971959561No Hit
AACATGTTCCAGAGCAGTTGGTAGAGTAAATGCCGAGAATCAAACAGAGT40410.4804588435459075No Hit
GATGTTTTGAGATTTTCAGTTTGTCTACATAAATTATCACTTAAGAGCTT40350.47974546738622537No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTCTTGGTGCCATTTATCGTTTTTG39000.46369450379337773No Hit
AGGAAATAATGAGCGAACCCCACTTCCCCACAACGGCACCCCACATGAAA38300.4553717819304197No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC38250.4547773017973512No Hit
CAGTACTTGTCCCTGAGGTAGTTTCTTTTCGAGAAGTTTTGGATGGAGGT37790.44930808457312166No Hit
CCAGATCCTTAACATTGGTCCGTATTTGAAGTCCCACTTTCTCATGGTTA37610.44716795609407534No Hit
CATGAGGGTAGTGTTGACAAAGATTAAAACAGAAAATGCTTTATGTTTAG35740.4249343991173159No Hit
GTCACTGACAACTGGCTTGTGCAACATTTTGACATGGAAGTCACAGACTA35250.4191084938132452No Hit
TACTGGTGTGGGTTATAGAAGAAACATGAGAGATTTAGTTCAAAAGGAAG35070.41696836533419884No Hit
ATGTTACCTTAAGATCAACCACCATGGACTGAACAAGTAGGAAAATGACA34890.4148282368551526No Hit
TTACCCACAGCTCCAAACATCTATGGCTGGTGTGTAACGTTCCTCTCCTA34020.4044842825397618No Hit
GTGGATTAACTGACGAAGGATTTTGATTTCACGAATGGCTGTGATTGGGA33510.3984205851824638No Hit
CAGATGGGCTGGAAGTAAGGAAACATGTAATGATAGGCGGACTCCCAGCA33190.3946159123308258No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG32060.3811806613234792No Hit
GTCCATGCTCTTGAAAATAATCATCTACTAACCTTTTTTATAAAACAGTG31550.3751169639661812No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA31540.3749980679395675No Hit
GACAGGTTCATGAGTGCTTCCCCCACATTCCTTCAGGTGACTGGTTCTTC31200.3709556030347022No Hit
GTGTCAATGGCACTGAACCCTGTTTCAGGCCCATCAGTGTTTCCATAAGT30700.3650108017040179No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACAATATCACC30700.3650108017040179No Hit
CTGAGGTCGCTCAGTTCCGGAGCTGCAACCCGGAGTCCCAGATGATCGGG30310.36037385666608407No Hit
ATTAGGATGTCTGGCACATGCACAGGACAACCAAGTTCAAGAACCTCTCA28800.3424205566474174No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA28280.3362379632635057No Hit
TTATCGCCATCCTTCTGAACTTAAGAAGAGCTCTAAAAAGCCAGTGTACA26160.31103200562140415No Hit
TATGAGAACACGCAGAGGGAACTTGGCCTCCTACTGCTGCTTTCAGAGAG26030.3094863572754262No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC25920.3081785009826757No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG25610.3044927241576514No Hit
CCAACTGGCCAAGACCTACCCTGTGCAGCTGTGGGTTGATTCCACACCCC25280.3005691552793997No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC24990.2971211705076028No Hit
AAGTACATATATTTAGCAGATCAGTTAACAGACAAAAGTCAACTTTACAG24900.2960511062680796No Hit
CTGTGTCACCAAGGGTTTCGTTGGGGCCATGAAGACCACCTTTCCTGAAG24670.29331649765596485No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGAATAAGCAGAAACTGCCATGCTC24000.28535046387284785No Hit
CGTCAGCTATTGAATTACTTTCCAAAAGAGAAATTTCATTGAATTTTTTA23720.2820213751276646No Hit
AGAAAAAGTTTCAGTTTTACCAATTTCCATTTTTACGTTTTTAGGTGAAG23320.27726553406311716No Hit
AAAAGCCCCTAAACCCCACTTCATTTTCATCTGTTAAATAACCAGAAGCA23090.27453092545100233No Hit
CACGCACACACAAGCAGATTGCTCAGATTACTAGAGAGCTCCGGAGGACG22930.2726285890251834No Hit
GCACCTGAGCTCAATGAGATTCATGACCGGTCTGTTTACCTGCTGCCTCC22810.2712018367058191No Hit
AATGCAGATGATTACCTCGCTCTGGCAAATGATTTCATTAAAGATTCAGA22680.2696561883598412No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA22570.26834833206709063No Hit
TTACTTTACCTTTCCAATTTGCTGAAGAGTGCAAACAATACATCATACTT22240.264424763188839No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT21420.2546752890065167No Hit
ACAAGTTGCAGGACTTTTTGCTGTTTCTTTTTCTACATTTTCATGTACTT20860.24801711151615025No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG20670.24575808701049018No Hit
AGAAAGAAATATATGGTAAGTTTCAAGAATACATCATACCTGTATAGGGT20610.24504471085080806No Hit
CTACCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGATCCCCCACA20290.24124003799917013No Hit
CGACTCAGAGACCGCTTGCTCAGGAAAGGGCTGCTGGACCGCCTTCGACC20100.23898101349351003No Hit
CTGAGTAAGTGGCAAGAAAATTACCTGCGTGCTGCATCAAAGTTGCTTTT19800.23541413269509948No Hit
ACACCGGATATCTGCTTCCTCACAGAGGTGGCGGAAACAGGACATGGCAA19560.23256062805637098No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTAGGCTGCAGCGACTTCGAACT18780.2232867379805034No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT18740.22281115387404868No Hit
TGCCTGAATGTTCTTCACCACCTTCATGCAAACGTAAAGTTGGTGGTACA18410.21888758499579702No Hit
GTCTCTGTATGGCAGCAAATGTGGCCCATGGCCTGCAGCAAAACGTCCTA17510.20818694260056522No Hit
TCAGATTGAGACCTCCTTTCCACCACCATGCGATTACTCACGATGTAATC17330.20604681412151887No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC17000.2021232452432672No Hit
CCAGTCCTCAGATAAATGGTGAAATAGACTTACTAGCAAGTGGCTCCTTG16670.19819967636501554No Hit
GAAAACTTTTATTGATTTATTTTTTGGGGGGAAATTTTTTAGGATCTGAT16580.19712961212549235No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC15320.18214871277216785No Hit
TCTGAGCACCAGTGGAGATTTTGTTAAGCAAACGGTGCCCTCAGAAAATA15060.17905741608021203No Hit
GTGTCCTGCTTATTTTTCTCACATTCTTCCGTACTGGCCTGGGAACTCTC15020.17858183197375727No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT14680.17453936706889192No Hit
ACTTTGTAATTCAACATTCATCGTTGTGTAAATTAAACTTCTCCCATTCC14510.17251813461645926No Hit
TGGATTTACACACTAACCTGGTAGAAATGCGACTAAAGACTGCATTGAAG14490.17228034256323188No Hit
GCAGTCTGGGGCCCTTTGCTCTGCCACACACAGTCGGGAAACAAGCATAG14280.16978352600434446No Hit
CCTGTCATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTA14210.16895125381804865No Hit
GTTCTGGAGTACGTATAGCAGTATTTTCTTCTCTTGTGAGCTGGTCTGAA14090.16752450149868442No Hit
AGAAACAGCTAATAAAAAGTTCTCCAAACTTACTGATTTTTTGTTTTCCT13910.16538437301963804No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCTGGAAAATAAACATGAC13800.1640765167268875No Hit
TCAAAAGGAGATACTTACACAACAGGAAAGACTTTGGGAAACACAACACT13690.16276866043413693No Hit
CGAGATGTTCATTTTCCCCAAACTCACAAAGAGAATTCTTCTCGTAGACG13150.15634827499699788No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT12400.14743107300097139No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGAC11470.1363737425258985No Hit
AATTACCTCCAGTTGCTCCTTCTGGGTAAAACCCTTCGTGCTTTTCCCTG11180.13292575775410162No Hit
TCCTTGGATGATGATGCTTTCACGGCTCCATTTCATAGGGATGGAATGCT11110.1320934855678058No Hit
ATTGTGTGTTCTTAAAGCAGGCATACTAATTTGAACAGAAACCTTCATTT10480.12460303589114355No Hit
GCAGGTGACCAACCACATAACTCGAGACAGGGACAGCGGGAGGCTCAAAC10470.12448413986452986No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA10460.12436524383791618No Hit
TCTGAGCACCAGTGGAGATTTTGTTAAGCAAACCGTGCCCTCAGAAAATA10330.12281959549193826No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT10190.12115505111934666No Hit
AGAACATTTCCTCAGAATTGTCCCAAAAGAGCTAGTTAAGGACAAAGTTG10120.12032277893305084No Hit
CACTCCACCCCCAAGAATATCATCTAGTGCTGAACAGAAGGTGATTATGA9730.11568583389511705No Hit
CCACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT9540.11342680938945703No Hit
ATTACTAAGTCATAAAAATAAACCAGGTAGAATATTTACCTTCACAAACT9160.10890876037813692No Hit
CATTCCTTGTTGTGCTCAGTACTGACTTTGGTATGTGTGTTCTGCTGTCC9110.10831428024506849No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC9020.10724421600554532No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT8730.1037962312337484No Hit
CGCTCCTACCTCCCCTCACCTACTCTGTCCTCACCCCCACTCCGCTCCCA8710.10355843918052102No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG8650.10284506302083891No Hit
TGTGATTATTCTGGAGAGGAAGATTAAGAGGACAAGCAGATTCAAGGTGA8640.10272616699422522No Hit
TTCTGACTTTCAGTAAGGCAATGAAAAATAATACTCTTACCAGCTACTTG8440.10034824646195149No Hit
TCTCCAGGTAATAAGGGTGGGAGGGGCAAAGGAGGCAACGTTGAAGTTTT8440.10034824646195149No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)