FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208208
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC2110210.135057250441866No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC86984.177553216014754No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG55122.647352647352647No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT49832.3932797971259507No Hit
AAAAGCAGTGGTCCAATGTATCTGCCATGGTTCTACATCTATTGTGATGG47112.2626412049488973No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC32711.5710251287174366No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA22491.0801698301698301No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAGTC19690.9456889264581572No Hit
ACGATGGTTGTATTTGTCACCAGTCCCCATCCACTGACATATAGTAGTGA19380.9307999692615078No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA12580.6042034888188734No Hit
GATTCCCGCGCCCAGAGTCCCACGACCCCCTCAGACCAAGTCTCTGCTAC10820.5196726350572505No Hit
GGGGTCAGAGGCAAGCAGAAACTGGCAGCTATGGAATGTGAGACCAAGTC10450.5019019442096365No Hit
GTTGTATCCGCTGCTTTGTCCCACCCTTATTACCTGGAGAAGACCAAGTC9880.4745254745254745No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA9590.4605970952124798No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT9550.4586759394451702No Hit
CCTCCCTGATGTACCACCAAGACCAAGTCTCTGCTACCGTAGTCGGTCTG8580.4120879120879121No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAGTCGGTC8490.4077653116114655No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAGT8470.4068047337278107No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC8130.39047490970567894No Hit
ATGAGCAAGTTGGGGTGTGGGGGTTGTTGACTTGTAGACCAAGTCTCTGC7810.37510566356720204No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC7580.36405901790517176No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAGTCGGT7350.35301237224314147No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAGTCG7320.35157150541765925No Hit
GAGTTTGATGCACAACTTCAAGGCACACTGACTTATGGTATCAAATAATA7150.3434065934065934No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC6820.32755705832628906No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA6520.313148390071467No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAG6100.2929762545147161No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTAGTC5950.285771920387305No Hit
TGACACACTTGCAGAAACAGTATTGGCTGATCGGTTTGAGAGATTGGTGG5170.24830938292476754No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTAGTCG5120.2459079382156305No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG4900.23534158149542767No Hit
TCATGCTGTTTACATTCACTAAGGCTACGGTAGCAGAGTCACAGTCACAG4850.23294013678629064No Hit
CAGCAGTTACCAGGGCACAGTAGCTCATGGTGGGGGCAGCAGACCAAGTC4770.2290978252516714No Hit
GAGAAAGCCCCCCTACTGCTACCGTAAGGGACAGTGATGAAATTAAAGAG4190.20124106662568203No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTAGTCGGT4140.198839621916545No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCCTGAAAACCAGAT4040.19403673249827094No Hit
CTCCCAAAATTCAAAATCCTCAGCCGTTACCTGTGTGTGAGACCAAGTCT3820.1834703757780681No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA3650.17530546376700223No Hit
CCTCCCTGATGTACCACCAAGACCAAGTCTCTGCTACCGTAGAGAAGGAG3420.16425881810497195No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTAGTCGG3370.16185737339583492No Hit
CCCATTTTCAAGAACTCTACCATGGTTTTATATGGAGACACAGGTGATAA3330.1599362176285253No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGGAGACCAAGTCT3320.15945592868669792No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT3210.15417275032659647No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC3190.15321217244294166No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT3120.14985014985014986No Hit
TCTGGGGTATCAGGTAGGTGTCCAGCTCCTGGCACTGGTAGAGTGCTACA3030.1455275493737032No Hit
TTTGCAGACCTTCTCAGGAGTCACTACTTCATTCCTGCTCTTAGTGTCTG2940.1412049488972566No Hit
CTCAGTCACACATGCCAGAGATTGCTCAGGAAGCAATGGAGGTAAGGGGA2930.14072465995542918No Hit
CTGTATGTAGTCGGTGCTGTGACTTGTTTGTGCTCATCTCTGTTCTGTAG2870.13784292630446476No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA2850.13688234842080996No Hit
GCAAACAGAAGGTTCCTTGAGCACCCGGAAACGGTCACGAGACCCAGAGG2820.13544148159532776No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT2820.13544148159532776No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTAGTCGG2790.13400061476984554No Hit
TTTGCTAATAGATGCCTAAGCCCAGAAAGGGTGCTTCTTCAACTAAAATA2770.13304003688619073No Hit
TGTTTCAGGAAGGAATGTTCCCAATAGTAGACATAAAAGTCTTCGCACAG2740.13159917006070854No Hit
AGAGGAACGTTACACACCAGCCATAGATGTTTGGAGCTGTGGGTAAGGTT2700.1296780142933989No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG2680.12871743640974412No Hit
TTTTGTGCGAAGGAAACCGATGGGGATCGGAACCGTAGCGGTTGAGCTGC2650.1272765695842619No Hit
TTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTA2610.12535541381695228No Hit
CCAGCTCACAAGAGAAGAAAATACTGCTATACGTACTCCAGAACATTTAA2580.12391454699147007No Hit
CCAGTGTTGTGTTTCCCAAAGTCTTTCCTGTTGTGTAAGTATCTCCTTTT2580.12391454699147007No Hit
TGTCATAATGAATGCCCCATCGATTGGTCAGGTAGACAGCAGCAAGCAAT2570.12343425804964266No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG2570.12343425804964266No Hit
CACGTTTTTCCCATCTAGCAATTCCATGCACTTCTCATTTCTGTTGAAGT2570.12343425804964266No Hit
TATTGCTTGAGCTGGCTTCTTTAAAAACATTTTCTCTAATGTTATTACGG2560.12295396910781525No Hit
GTAACTTGATTTGCTGTTGTATTTGCTACCGTAACCCTCAATTTGGAAGC2520.12103281334050564No Hit
ACCTCCATCCAAAACTTCTCGAAAAGAAACTACCTCAGGGACAAGTACTG2480.11911165757319603No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC2470.11863136863136862No Hit
TTACCCAGTACCATCAATGCTGTTAACAGTTCTTCATCCTTTTTCCAGCT2470.11863136863136862No Hit
GCCAAACGAAAATTATGGCAGGTTGTTACGAGGCATTGGATGATTCAGAG2470.11863136863136862No Hit
CTAACAGTACTCGGCCTGCTCGCTGGTATACCAAACTTGGATTCTTTCCT2450.11767079074771383No Hit
TTAACTACAATTCCCAGCAAGCCGCGGAGCCAACCTGGGCCTCTGCCGGC2440.11719050180588643No Hit
TCGGCTATAAAAAAGATAATGGAAAGGGATGACACAGCTGCAAAAACACT2440.11719050180588643No Hit
CTGTGGTGCCACCTAAGCTCTTAAGTGATAATTTATGTAGACAAACTGAA2430.11671021286405901No Hit
CTTTCTTCAGAAGCTCCACCCTATAATTCTGAACCTGCAGAAGAATCTGA2410.1157496349804042No Hit
TGAAGTGGACTGTTTATCTTCTTCACTTACTAGAGACAATTCAATGTCTG2390.11478905709674941No Hit
CTTTTTCTGGTTGGGCAGTTGGTGGAATTAATACACTGTCTTCATTAATT2380.114308768154922No Hit
TATCAACTGACCCAGGCAAAATATAAATTACCTTGACAAGTTGATGAAGT2380.114308768154922No Hit
CCAAATAACAAGTGTTGGAAGCAGGGAAGCTCTTCATCCTCACTAGATAA2380.114308768154922No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG2360.11334819027126719No Hit
TGAATGTAGCACGCATTCACATAAGGTTTTTGCTGACATTCAGAGTGAAG2360.11334819027126719No Hit
TCAATGCAGAGGTTGAAGATGGTATGTTGCCAACACGAGCTGACTCTGGG2340.1123876123876124No Hit
TGGTAAATTCATCTGCTTTCTCTGGATTTAGTACAGCAAGTGGAAAGCAA2300.11046645662030279No Hit
CTAATGAGCGGACGGCAAAGAACGCTTTTTCAGACGTGGGGCTCAAGTAT2280.10950587873664797No Hit
TCAGATATAAAAGAAGAGGTCTTGGCTGCAGCATGTCACCCAGTACAACA2270.10902558979482056No Hit
AGCAAGCTGTGACGGGTAAGCTTTATAAGGAAAGTAAATCTTCACCCCAC2270.10902558979482056No Hit
GGTCCAGCAGCCCTTTCCTGAGCAAGCGGTCTCTGAGTCGGAGTCCACTC2270.10902558979482056No Hit
TCAAAGGGCTCCACTGGTTTTTCTGAGCAGGACTTCACTTTTTCAAGCTT2270.10902558979482056No Hit
CCACAGAGATAGCACCTAGAAAGCTGAATTAAGCAAGGAGGGGCAGAACA2260.10854530085299316No Hit
TTCTTCTCTTGGAAGGCTAGGATTGACAAATTCTTTAAGTTCACTGGTAT2250.10806501191116577No Hit
CCAAAATATGTCTGGATTGGAGAAAGTTTCTAAAATATCACCTTGTGATG2240.10758472296933834No Hit
AAAGCAGAAGCCTTAGAAACTCTGCAAATTATCAGAAGAGAATGTCTCAC2220.10662414508568355No Hit
CACCCTTGAACAGTAAAAGCGAATCTTTACCTGTGTCAGACAAAACTGCT2200.10566356720202875No Hit
ATTTACCCTACCAATTGCCCAGTGCGGGACTACCAGCTGCACATTTCCCG2200.10566356720202875No Hit
GTCCTCCAGAGCACAAACCTGTGGCAGATACACACTGGTCCAGCCTTAAC2200.10566356720202875No Hit
TATTTGTTCAGGGCTCTGTGTGACACTCCAGGTGTGGATCCAAAGCTTAT2190.10518327826020132No Hit
GTTACTCTGTTTGATTCTCGGCATTTACTCTACCAACTGCTCTGGAACAT2190.10518327826020132No Hit
CGGCAGTTGTGTCTAGAGAATGGCGGGTTCTGCACCTCCGCGGGCGCCCT2180.10470298931837393No Hit
GCAACAGCTTCATTGCTGAGGGACTAGACATCACTCGCCTGTAACTTGAA2170.10422270037654653No Hit
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA2160.10374241143471913No Hit
CCTATCTCATCGTCTTATATTGGCTTCACTGTCTTTCAACAGGCCTTGCT2160.10374241143471913No Hit
TCTGAACTCCAAGATCAAATCACCCGTGATGCTAATAGTTTTAAATCTCG2160.10374241143471913No Hit
GTAAAATGTGCTCCCCAAAAGCATAAACATTTAGCTCACTTCTATAAATA2160.10374241143471913No Hit
GCCACAAAGTAAAAATGTTGTGTGTTTACTTTTTTGCATCTTGGCAGGCT2160.10374241143471913No Hit
AATGTTATTAAAGAACCGTGTGTGTTATTAACAGAGTGTCAGTTTACAAA2160.10374241143471913No Hit
GTCTCAGAACAAACCTGAGATGCATGACTACTTCCCATAGGCTGTTCTAA2150.10326212249289171No Hit
GTGGTGGTATACGATATGGGTTTTGTAAAAGTCCATGTTTATTTGGAGTA2150.10326212249289171No Hit
GGTATCAGATGCTTCATTACAAAACGCAAGACAAGTGTTTTCTGAAATAG2150.10326212249289171No Hit
GGAAACACTGATGGGCCTGAAACAGGGTTCAGTGCCATTGACACTGATGA2140.10278183355106431No Hit
TATTTGTTCAGGGCTCTGTGTGAGAGAGATGGCCAGAAAGGGTGCTTCTT2140.10278183355106431No Hit
TCCTCTCTTAGATTTGGCTTTCTGTTTTGTTTTGTTTTCCTGTGGCTTTT2120.10182125566740952No Hit
AGTAAGGGTGGCATCAATCTTTAATGATGAAATAATGGTTTCTGATTGAG2120.10182125566740952No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA2120.10182125566740952No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAATTATTTTACCAGATGATTCA2120.10182125566740952No Hit
CTTAAATGTTGAAGGTGGTTCTTCAGAAAATAATCACTCTATTAAAGTTT2120.10182125566740952No Hit
GCATATGGAGAAACTTGAGGAATTTTTAATTTTGTTTTGGATAGCCTATT2110.10134096672558211No Hit
TGGTGATAGATGACTTGCTGCTTCCAGTAAATAAGGTGGGGTACTGTACT2110.10134096672558211No Hit
TTCTGATGAGCCAAGTCTCTGTGACTGTGATGTACATAAACATAATCAAA2100.1008606777837547No Hit
CCAGTGGCGACCAGAATCCAAATCAGGCCTTCTTACTTTATTTGCTGGAG2090.10038038884192729No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)