FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208208
Sequences flagged as poor quality0
Sequence length150
%GC40

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG2110910.138419273034657No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA86814.1693883040036885No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT54592.621897333435795No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT49662.385114885114885No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGGCAGATACATTGGACCAC40851.9619803273649428No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG30891.4836125413048489No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT22451.0782486744025206No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA19600.9413663259817107No Hit
CCCCATGTCCAGGCTCACTACTATATGTCAGTGGATGGGGACTGGTGACA19120.9183124567739953No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT12520.601321755167909No Hit
TGGTGGTACATCAGGGAGGTGTAGAACCATGTCGTCAGTGTAGATCTCGG12000.576346730192884No Hit
GAGGGGGTCGTGGGACTCTGGGCGCGGGAATCTGTAGAACCATGTCGTCA10980.5273572581264889No Hit
CACATTCCATAGCTGCCAGTTTCTGCTTGCCTCTGACCCCTGTAGAACCA10510.504783677860601No Hit
TCTCCAGGTAATAAGGGTGGGACAAAGCAGCGGATACAACTGTAGAACCA9760.4687620072235457No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC9520.457235072619688No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT9520.457235072619688No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC8580.4120879120879121No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG8460.40632444478598323No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG8120.38999462076385155No Hit
ACAAGTCAACAACCCCCACACCCCAACTTGCTCATTGTAGAACCATGTCG7710.370302774148928No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG7540.36213786213786214No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT7390.3549335280104511No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT7360.35349266118496886No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTGCCTTGAAGTTGTG7050.33860370398831935No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG6910.33187965880273573No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT6380.3064243448858833No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA6100.2929762545147161No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA5870.2819296088526858No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT5120.2459079382156305No Hit
GGCACTTTTCTTCCCCGCTTACTCTAATCACTTACCCACTGAGAACAAGG4950.23774302620456467No Hit
GCTGCCCCCACCATGAGCTACTGTGCCCTGGTAACTGCTGTGTAGAACCA4840.23245984784446322No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT4770.2290978252516714No Hit
GACGTGGCCATCACAATAGATGTAGAACCATGGCAGATACATTGGACCAC4200.2017213555675094No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT4190.20124106662568203No Hit
AACCTACCTGTGACTGTGACTCTGCTACCGTAGCCTTAGTGAATGTAAAC3990.19163528778913394No Hit
CACACACAGGTAACGGCTGAGGATTTTGAATTTTGGGAGTGTAGAACCAT3900.18731268731268733No Hit
TCTCTTTAATTTCATCACTGTCCCTTACGGTAGCAGTAGGGGGGCTTTCT3850.1849112426035503No Hit
GCTGTCTACCTGACCAATCGATGGGGCATTCATTATGACATGAAGATCAG3820.1834703757780681No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG3430.16473910704679937No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC3420.16425881810497195No Hit
CCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT3270.1570544839775609No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG3200.15369246138476908No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT3110.14936986090832244No Hit
CAGAAATGACATCTACCTGTTACTCACAGAAACCACACACCAAAGGAACA3040.1460078383155306No Hit
CCAGTCCTCAGATAAATGGTGAAATAGACTTACTAGCAAGTGGCTCCTTG2930.14072465995542918No Hit
CCTGGAGTCGATTGATTAGAGCCTAGTCCAGGAGAATGAATTGACACTAA2890.13880350418811957No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG2770.13304003688619073No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT2770.13304003688619073No Hit
GGATTCATTCATGCCATAGTGTGTTTATTTATACTTTAAGGTAACAAGTC2740.13159917006070854No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC2730.13111888111888112No Hit
GTCACTGACAACTGGCTTGTGCAACATTTTGACATGGAAGTCACAGACTA2710.1301583032352263No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT2690.1291977253515715No Hit
GGTTGCTGAGGTGGTGTGATTGCCTTTGGTTCTGGAGAGTCTGGGGGAGA2640.12679628064243448No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT2630.1263159917006071No Hit
CACGCACACACAAGCAGATTGCTCAGATTACTAGAGAGCTCCGGAGGACG2610.12535541381695228No Hit
ATATTCAAGGAGATGTCCGATTTTAAATCTTGACCTAGAGTCATTTTTAT2600.12487512487512488No Hit
CTGAGGTCGCTCAGTTCCGGAGCTGCAACCCGGAGTCCCAGATGATCGGG2560.12295396910781525No Hit
GCACAAAGGCCCCATTCTGGCACTGACCTATTGCATCCCCAGCCCCAGAC2530.12151310228233306No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG2500.12007223545685085No Hit
GCAGATGAGACTGACTTATGAAGCTTCCCTATACTACATTTACATATATC2500.12007223545685085No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACCTCCATGATCTCCAACAAAG2440.11719050180588643No Hit
ACTTGCTTTCCACTTGCTGTACTAAATCCAGAGAAAGCAGATGAATTTAC2440.11719050180588643No Hit
GTTGTAAAATTTCTTCACTCTGAATGTCAGCAAAAACCTTATGTGAATGC2430.11671021286405901No Hit
GAGATTGGTACAGCGGCAGAGTCAGCCCTTGCTCTTTGAATATTATTGGA2400.11526934603857682No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC2390.11478905709674941No Hit
GCATGCCATTCAAAGGATTTTACAAACAATGACATGAACCAAGGAACTTC2360.11334819027126719No Hit
TTATCGCCATCCTTCTGAACTTAAGAAGAGCTCTAAAAAGCCAGTGTACA2360.11334819027126719No Hit
GTGTACACAGTGTCCACCAAGGTCCTGAGATCCTCATTTCTCTTCCTTGT2350.1128679013294398No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG2340.1123876123876124No Hit
GCCTCTAGAAATCATGACTAGGTTTGACAGAACATCCTTGGAAGTAGGAG2340.1123876123876124No Hit
GTAGTCAGTGCATTCTACAACAGCGCGCATGTTAGCAAGTTCATCAACCA2320.11142703450395758No Hit
CGACAAGCAACACATCATCGTCCGACTCCAGCTGAGCCTCCGCTGCTGCT2310.11094674556213018No Hit
AGAAACAGCTAATAAAAAGTTCTCCAAACTTACTGATTTTTTGTTTTCCT2300.11046645662030279No Hit
AAAAGCCCCTAAACCCCACTTCATTTTCATCTGTTAAATAACCAGAAGCA2290.10998616767847536No Hit
TTCTTTGTAAGGCGTGTCTCAATATTTGACATTTTCATTTCTGTTGTAGT2290.10998616767847536No Hit
GAGCTCGGGTCATCAGAGAATAGTGCCTCTAGTCCTCCCCGTTTCAAAAC2270.10902558979482056No Hit
GTTCTGGAGTACGTATAGCAGTATTTTCTTCTCTTGTGAGCTGGTCTGAA2210.10614385614385614No Hit
CACAGTGCTCTGGGTTTCTCTTATCAACACGAGGAAGTATTTTTGATACA2210.10614385614385614No Hit
GCAGTCTGGGGCCCTTTGGCAGGCCCTGGTTGCCCTGAGGCGGGTGCTGC2200.10566356720202875No Hit
AGCTTCATCATACCTTTCACTAAGACAAGAATTTAGAAGTGGACAGGAAA2170.10422270037654653No Hit
CAGCTGTGCCATTTGAGGGATGCTCAGGTCGGTTTGGCTCTGCAGCAGGT2160.10374241143471913No Hit
AAGAGGCTTCCAAATTGAGGGTTACGGTAGCAAATACAACAGCAAATCAA2150.10326212249289171No Hit
CCAGATCCTTAACATTGGTCCGTATTTGAAGTCCCACTTTCTCATGGTTA2150.10326212249289171No Hit
GGAAGGTAAAGAACCTGCAACTGGAGCCAAGAAGAGTAACAAGCCAAATG2150.10326212249289171No Hit
GAAAGCCAGGGAGTTGGTCTGAGTGACAAGGAATTGGTTTCAGATGATGA2090.10038038884192729No Hit
GTGTACTGCCCTCCTCCCACCTGTCTCTGACCAACATTGGTCTTTTGTGA2090.10038038884192729No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)