FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62417
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC765712.26749122835125No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG700511.222904016533958No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC62319.982857234407293No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTACATTGACGAACATGGTTCT58809.42051043786148No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA27864.463527564605797No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC22733.6416360927311473No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC19683.15298716695772No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA14212.2766233558165245No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA13822.2141403784225453No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT12852.0587339987503404No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAGTC12822.0539276158738806No Hit
TCGGCTATAAAAAAGATAATGGAAAGGATTGTGAAAGCATCATCATCCAA7701.2336382716247176No Hit
AGTTCCATTTAGGTTGCTGGTGGTGAAGAACATTCAGGCAAGACCAAGTC7571.2128106124933913No Hit
GCTTAGATTCCTCTTTGGAGTCAGTGTACCTCTCACAATATTAAATGCAC7441.1919829533620647No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC6401.0253616803114536No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA6200.9933191278017207No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA6070.9724914686703943No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAGTCTTGG5800.9292340227822549No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAGTCTTG5570.8923850873960619No Hit
GCCGGAACAGCCCCAGCTCAGGACAGGTGTTTATCCTGGGACAACCTGGA5490.8795680663921689No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT5430.869955300639249No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC5050.8090744508707565No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA4770.7642148773571303No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTAGTC4330.6937212618357179No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAGT4260.6825063684573113No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAGTCT4250.6809042408318247No Hit
ATGAGCAAGTTGGGGTGTGGGGGTTGTTGACTTGTAGACCAAGTCTCTGC4020.6440553054456318No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAG3960.6344425396927119No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG3440.5511319031674063No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC3110.498261691526347No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT2750.44058509700882775No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT2640.4229616931284746No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTAGTCTT2550.4085425444990948No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTAGTCT2530.40533828924812154No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTAGTCTTG2410.38611275774228176No Hit
CCTCAGGGCCTCAGAGAAAGGGTGGGGAAACTTGAAAGTAGACCAAGTCT2300.36848935386192866No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG2270.3636829709854687No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT1800.2883829725875963No Hit
CCAGTGTTGTGTTTCCCAAAGTGCTGGGATTACAGACATGAGCCATGGTG1620.2595446753288367No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTAGTCTT1600.2563404200778634No Hit
CTCCCAAAATTCAAAATCCTCAGGTTCTACACCAAATAACAAGTGTTGGA1520.24352339907397022No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT1430.2291042504445904No Hit
TCTACCCACTCTCTTTTCAGTGCTTACGGTAGCAGAACTGTCAAATGACC1220.19545957030937086No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG1140.18264254930547766No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTG1120.17943829405450437No Hit
AAGTGGTTCTGGATTAGCTGGATTATCAGTGCGCTTTTCCCAACACCACC1070.17142765592707115No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA1060.1698255283015845No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTAGTCTTGGCTCATC1050.16822340067609787No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTAGTCTTGGC990.15861063492317798No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTAGT910.1457936139192848No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAGAGACCAAGTC890.14258935866831152No Hit
GCTATTCCTACCATTCTGATGAGGTAGACCAAGTCTCTGCTACCGTAGTC880.14098723104282487No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACGAGA880.14098723104282487No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATAAGACCAAGTCTC870.13938510341733823No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC860.1377829757918516No Hit
ACACCACTCTCTAAATCAAACACATTGGAGACCAAGTCTCTGCTACCGTA860.1377829757918516No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT800.1281702100389317No Hit
CACAAAGGTTTTTATAAGTTCTGTGGATCTAGACCAAGTCTCTGCTACCG790.12656808241344505No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTAAGAC780.12496595478795841No Hit
AAGTCCTCCATTTCTGTATCCATGCGAGACCAAGTCTCTGCTACCGTAGT730.11695531666052518No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC700.11214893378406524No Hit
ATTTAACACCTAGCCAAAAGGCAGACCAAGTCTCTGCTACCGTAGTCTTG680.10894467853309195No Hit
TGATAAATGCACCTGTTTGCTGTCACATTCAGAGACCAAGTCTCTGCTAC660.10574042328211865No Hit
AACAATCGACAGGCTAGAAGTTGAGACCAAGTCTCTGCTACCGTAGTCTT660.10574042328211865No Hit
AGTTCCATTTAGGTTGCTGGTGGTGAAGAACATTCAGGCGAGACCAAGTC640.10253616803114537No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)