FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62417
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG761412.198599740455325No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT676210.833587003540703No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG58289.337199801336174No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTTCGTCAATGTAGAACA54398.71397215502187No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT27574.417065863466683No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA22673.6320233269782274No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA19513.1257509973244466No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT14072.2541935690597112No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT13802.210936123171572No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC12882.0635403816268005No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA12752.042712722495474No Hit
TGCCTGAATGTTCTTCACCACCAGCAACCTAAATGGAACTTGTAGAACCA7631.2224233782463112No Hit
TCCTTGGATGATGATGCTTTCACAATCCTTTCCATTATCTTTTTTATAGC7251.1615425284778187No Hit
TGAAGTGCATTTAATATTGTGAGAGGTACACTGACTCCAAAGAGGAATCT6701.073425509076053No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG6351.0173510421840204No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT6040.9676850857939343No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT5810.9308361504077415No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC5700.9132127465273884No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT5550.8891808321450886No Hit
GAGCTCGGGTCATCAGAGAAATCTATTGTCCAGGTTGTCCCAGGATAAAC5240.8395148757550026No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT5040.8074723232452696No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG5000.8010638127433232No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT4560.7305701972219106No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA4410.706538282839611No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT4260.6825063684573113No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG4190.6712914750789047No Hit
ACAAGTCAACAACCCCCACACCCCAACTTGCTCATTGTAGAACCATGTCG4040.6472595606966051No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA3930.629636156816252No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT3430.5495297755419196No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG3120.49986381915183364No Hit
CGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTTCGTCAATGTAGAACA3110.498261691526347No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG2680.4293702036304212No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA2600.416553182626528No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC2590.41495105500104135No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT2550.4085425444990948No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT2430.38931701299325505No Hit
CTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG2360.3781021196148485No Hit
ACTTTCAAGTTTCCCCACCCTTTCTCTGAGGCCCTGAGGTGTAGAACCAT2270.3636829709854687No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT2220.35567233285803546No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT1810.28998510021308294No Hit
GAGGGGGTCGTGGGACTTGGCCAGGCACCATGGCTCATGTCTGTAATCCC1640.26274893057980997No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC1600.2563404200778634No Hit
CCCTGCTTCCAACACTTGTTATTTGGTGTAGAACCTGAGGATTTTGAATT1480.23711488857202362No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT1430.2291042504445904No Hit
TGGTCATTTGACAGTTCTGCTACCGTAAGCACTGAAAAGAGAGTGGGTAG1160.18584680455645097No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT1090.17463191117804444No Hit
CAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT1090.17463191117804444No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT1020.16341701779963794No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG990.15861063492317798No Hit
GTTGATACAAAACAAGCCCACGAACTGGCTAAGAGTTACGGGATTCCATT990.15861063492317798No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG960.15380425204671805No Hit
CTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA910.1457936139192848No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG910.1457936139192848No Hit
ACCTCATCAGAATGGTAGGAATAGCTGTAGAACCATGTCGTCAGTGTAGA880.14098723104282487No Hit
TATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG870.13938510341733823No Hit
CCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT860.1377829757918516No Hit
CGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT860.1377829757918516No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG850.13618084816636492No Hit
AGATCCACAGAACTTATAAAAACCTTTGTGTGTAGAACCATGTCGTCAGT800.1281702100389317No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC800.1281702100389317No Hit
TAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA780.12496595478795841No Hit
TGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTTCGTCAATGTAGAACA740.11855744428601181No Hit
CGCATGGATACAGAAATGGAGGACTTTGTAGAACCATGTCGTCAGTGTAG700.11214893378406524No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG690.1105468061585786No Hit
GCCTTTTGGCTAGGTGTTAAATTGTAGAACCATGTCGTCAGTGTAGATCT660.10574042328211865No Hit
AGGAAATAATGAGCGAACCCCACTGTAGAACCATGTCGTCAGTGTAACCC640.10253616803114537No Hit
CGCCTGAATGTTCTTCACCACCAGCAACCTAAATGGAACTTGTAGAACCA640.10253616803114537No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)