FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727745
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAAATTTCACTCCACTTACCTACCAGTCAAATGTCCATGGATCATAGAC14848420.403300606668545No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCGTACATTGTATTGTCGTTTTA711589.777875492102316No Hit
CCGGTACAAACCTTTCATTGTATTGTCGTTTTAGTTCAACCAGACCAAGT616918.477007743096825No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCGTAGAGAAGGAGAGTTTGCCA359654.941978302839593No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCGTAGTTGGGAGGGAATTGGAA244383.358044369937272No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCGTACGAGATAGTGTTCAAGAA224553.0855588152443505No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCGTACTCCAAGGAAGTTGTACC210442.891672220351909No Hit
TTAAATTTCACTCCACTTACCTACCAAGGGCCTGGTTTAAGGCCCTGTAT183802.525609932050375No Hit
TTCAACGTTGCCTCCTTTGCCCCTCCCACCCTTATTACCTGGAGATGATG120481.6555249434898212No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA94531.2989439982411422No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCGTAGTGCAGATAATGACAAGG73331.0076331682113926No Hit
CCTCCCTGATGTACCACCAACTTTACGTTTGCATGAAGGTGGTGAAGAAC53810.7394073473538121No Hit
TCGTTAGCATTAACACTTTCAGGTACACTGACGACATGGTTCTACATCTA53710.7380332396649926No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCGTAGCAAATTTTTAGATCCAG53380.7334986842918879No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCGTAACATTGTATTGTCGTTTT41940.5763007646909288No Hit
TTCCATGAAGCAAACGCTGATGATTTGTCCCTGTCTGAGACCAAGTCTCT40980.5631093308782609No Hit
TTCAACGTTGCCTCCTTTGCCCCTCCCACCCTTATTACCTGGAGAAGACC40470.5561013816652811No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCCTGAAAACCAGAT39610.5442840555414328No Hit
TTAAATTTCACTCCACTTACCTACCAGTCAAATGTCCATGGATCAGAGAC39460.5422228940082034No Hit
TTCAACGTTGCCTCCTTTGCTGAAGTGCCTGAAAACCAGATGAGACCAAG39370.5409861970882658No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCGTATCTATTGTGATGGCCACG37020.5086946664010058No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTAGACCAAG35870.49289242797958077No Hit
ATTTAACACCTAGCCAAAAGGAAGTCTGTTTCCACAGACCAAGTCTCTGC35140.4828614418511979No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTAGCATAATC34750.4775024218648016No Hit
CCTCCCTGATGTACCACCAAGACGAGAAGGAGAGTTTGCCACCAAGACCA33120.4551044665370425No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCGCGAGATAGTGTTCAAGAAAT32490.4464475880974792No Hit
CGGGAGCCCCTTCACTTCAGCAAATTTTTAGATCCAGACTTTCAGCAGAC32280.4435619619509581No Hit
ATTATCCAGATGAATTTACAAAACTGTACCAGATCCCACAGACTGATATG32160.44191303272437465No Hit
ATACCACTGACGGCCAGGTAGAAGACCTCTCAATTTTGAATGGTGGTCAT31780.4366914235068602No Hit
TATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTGA31240.4292712419872345No Hit
ATTTAACACCTAGCCAAAAGGCAGACCAAGTCTCTGCTACCGTAGCATAA30460.41855320201444185No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC30430.418140969707796No Hit
GGTAGGATGTGATATTCCTTCTAGTGGAAATACCAGTCAAATGTCCATGG30220.41525534356127486No Hit
GGATGCAGAGCTTCTTCCCACAGACTGATATGGCTGAGACCAAGTCTCTG29560.4061862328150657No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTAGC27230.37416952366556966No Hit
TTCAACGTTGCCTCCTTTGTGGAATTATGGATGGAAGACCAAGTCTCTGC27020.37128389751904856No Hit
TGTACTTGAAAAAGGACTTTAGAGCAGTCAGAGGACCAGGAGACCAAGTC26510.36427594830606874No Hit
AAACGTGATGAGACCCGAGTTATGATCTTCTCTTCATTTCGAGATAGTGT26430.3631766621550131No Hit
AAAAGCAGTGGTCCAATGTATCTGCATAAATCCTGTCATTCTGTTGAAGG26300.36139032215954764No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG25730.3535579083332761No Hit
TCAAATGTAGCAAATCAGAAGCCCTTTGAGAGTGGAAGTGACAAAATCTC25520.35067228218675495No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCGGAGAAGGAGAGTTTGCCACC24480.3363815622230314No Hit
GTCCTCCAGAGCACAAACCTGTGGCAGATACACACTGGTCCAGCCTTAAC24220.33280888223210053No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCGACATTGTATTGTCGTTTTAG23830.3274498622457042No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA23780.3267628084012944No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC23150.3181059299617311No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA22500.30917422998440386No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCACATTGTATTGTCGTTTTAGT22210.3051893176868271No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCGAGCAAATTTTTAGATCCAGA21750.29886842231825705No Hit
GTCCTCCAGAGCACAAACCTAGTCATGATTTCTAGAGGCAGACCAAGTCT21300.29268493771856896No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCCGTACATTGTATTGTCGTTTT21240.29186047310527724No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCGTATACCAGTCAAATGTCCAT21020.2888374361898742No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT20640.2836158269723598No Hit
AAAAGCAGTGGTCCAATGTATCTGCTCCTAGCTAACATAATTGCAGACCA19520.2682258208575806No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCGTACCATTGTATTGTCGTTTT19380.2663020700932332No Hit
AAAAGCAGTGGTCCAATGTATCTGCTCTATTGTGATGGCCACGTTAGACC18670.25654590550261425No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCGTAGGTTGGGAGGGAATTGGA18530.2546221547382668No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCGTATTAATCTTTGTCAACACT18120.24898831321410658No Hit
TTTTGTGCGAAGGAAACCGATGGTTCTACATCTATTGTGATGGCCACGTT17980.24706456244975916No Hit
TTCAACGTTGCCTCCTTTGCCCCTCCCACCCTTATTACCTGAAGATGATG17940.24651491937423137No Hit
GGAAACACTGAAGTGCCTGAAAACCAGATGAGACCAAGTCTCTGCTACCG17640.24239259630777266No Hit
CAGCCTCTGGCATTCTGGAACTGTTATTATCCCATCAAATGAAGACCAAG17160.2357968794014387No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC16540.22727741173075733No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCGTTACTCCAAGGAAGTTGTAC16490.22659035788634754No Hit
CCGGTACAAACCTTTCATTGTAATTTTTCAGTTTTGATAAGTGCTTGTTA16420.22562848250417383No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC15750.21642196098908273No Hit
GGAAACACTGATGGGCCTGAAAACCAGATGAGACCAAGTCTCTGCTACCG15320.21051329792715856No Hit
TTCAACGTTGCCTCCTTTGCCCCTTCCACCCTTATTACCTGGAGATGATG15100.2074902610117555No Hit
AAAAGCAGTGGTCCAATGTATCTGCTCCGTATACCAGTCAAATGTCCATG15060.20694061793622764No Hit
AAAAGCAGTGGTCCAATGTATCTGCACGACATGGTTCTACATCTATTGTG14640.20116936564318544No Hit
CCAGAGACCAGACTCCAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTG14590.20048231179877568No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT14340.19704704257672676No Hit
CTGGCTGAGCACATAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCT13480.18522971645287842No Hit
TTCAACGTTGCCTCCTTTGCTTCAGAGCAGTCAGAGGACCAGGAGACCAA12810.17602319493778726No Hit
AGGACAAAATGTTTCACTTTTGGATAAGAGGGAGTAGGGGGAGACCAAGT12550.1724505149468564No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA12480.1714886395646827No Hit
CCGGTACAAACCTTTCATTGTACACTGACGACATGGTTCTACAAATCTCC12280.16874042418704355No Hit
ATTTAACACCTAGCCAAAAGGCAGAAATTACAGAACTTTCTACTATATTA12200.16764113803598787No Hit
AGGACAAAATGTTTCACTTTTGGGTAAGAGGGAGTAGGGGGAGACCAAGT12020.16516774419611266No Hit
CAGCCTCTGGCATTCTGGAAAAGCCAGAATTTACAGCTCTCCAGACCAAG10820.1486784519302778No Hit
TCAAATGTAGCAAATCAGAAGCCCTTTGAAAGTGGAAGTGACAAAATCTC10780.14812880885474994No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATTGATAGC10760.147853987316986No Hit
TCTAAGCATAGCATTCAATTTTGGCCACTACAAATCATTCTCCTTAAGAC10590.14551800424599276No Hit
CCGGTACAAACCTTTCATTGTTACGGTAGCAAATTTTTAGATCCAGACTT10560.14510577193934687No Hit
GTTAGAAGGCTGGCTCCCACAGACTGATATGGCTGAGACCAAGTCTCTGC10550.14496836117046494No Hit
ACGTTAGCATTAACACTTTCAGGTACACTGACGACATGGTTCTACAAATC10550.14496836117046494No Hit
ACCAGTTTGCTCAATGGTGCAGATAATGACAAGGAATATCTAGTAGACCA10540.14483095040158298No Hit
TTAAATTTCACTCCACTTACCTACCAACTGCTCTGGAACATGTTAGACCA10330.14194532425506187No Hit
AGTTTTTGTGTTGATGAAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCT10120.13905969810854077No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA10040.1379604119574851No Hit
GTGCAGGCTGATTTTCTTTTTCCAAATGTAATTTTTTAGTACATGGTACA9820.13493737504208203No Hit
ACCAGTTTGCTCAATGGTGTAGATAATGACAAGGAATATCTAGTAGACCA9630.13232657043332485No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAG9290.12765460429133832No Hit
AGTTTTTGTGTTGATGAAGAGGAGTCAGAGGACCAGGAGACCAAGTCTCT9160.12586826429587286No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCGTATTTTTCCAAATGTAATTT9150.1257308535269909No Hit
ACCAAAGAAGTCCACGAATGGTTACACTGACGACATGGTTCTACAAATCT8880.12202076276717808No Hit
TTCAGAAGACAATGTTGATGTTCATGATACACTGACGACATGGTTCTACA8760.12037183354059458No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCGTAAGAGCAGTCAGAGGACCA8590.11803585046960131No Hit
TCGTTAGCATTAACACTTTCAGGTTGCAAAACCCCTAATCTAGACCAAGT8530.11721138585630957No Hit
GTTGCCTTATTAACGGTATCTTCAGAAGAATCAGATCCTAAAAAATTTCC7700.10580629203910712No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)