FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727745
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGATCCATGGACATTTGACTGGTAGGTAAGTGGAGTGAAATTTAATGTA14702220.20240606256312No Hit
GGTTGAACTAAAACGACAATACAATGTACGGTAGCAGATACATTGGACCA706339.705734838439287No Hit
GGTTGAACTAAAACGACAATACAATGAAAGGTTTGTACCGGTGTAGAACC614998.45062487547149No Hit
TGGTGGCAAACTCTCCTTCTCTACGGTAGCAGATACATTGGACCACTGCT342954.712502318806725No Hit
TTTCCAATTCCCTCCCAACTACGGTAGCAGATACATTGGACCACTGCTTT236473.2493524517516437No Hit
TGCAATTTCTTGAACACTATCTCGTACGGTAGCAGATACATTGGACCACT214002.940590454073886No Hit
GGTTGAACTAAAACGACAATACAATGACCACCATTCAAAATTGAGAGGTC211122.901016152635882No Hit
AAGACGGTACAACTTCCTTGGAGTACGGTAGCAGATACATTGGACCACTG202972.789026375997087No Hit
CATGAGGGTAGTGTTGACAAAGATTAAAACAGAAAATGCTTTATGTTTAG147812.0310685748442103No Hit
ACTAGATATTCCTTGTCATTATCTGCACTACGGTAGCAGATACATTGGAC70250.96531065139575No Hit
GCTGAAAGTCTGGATCTAAAAATTTGCTACGGTAGCAGATACATTGGACC52660.723605108932387No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTACCTGAAA52140.7164597489505252No Hit
CATCTGGTTTTCAGGCACTTCAAATGTACTCTTCTGCAATATGTAGCTTG50930.6998330459158084No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT41750.5736899600821717No Hit
GGTTGAACTAAAACGACAATACAATGTTACGGTAGCAGATACATTGGACC41690.5728654954688799No Hit
CAGACAGGGACAAATCATCAGCGTTTGCTTCATGGAATGTAGAACCATGT40990.563246741647143No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC40280.5534905770565239No Hit
TCTCCAGGTAATAAGGGTGGGAGGGGCAAAGGAGGCAACGTTGAATGTAG40070.5506049509100028No Hit
CTGATCCATGGACATTTGACTGGTAGGTAAGTGGAGTGAAATTTAATGTA39210.5387876247861545No Hit
CATCTGGTTTTCAGGCACTTCAGCAAAGGAGGCAACGTTGAATGTAGAAC39170.5382379817106266No Hit
GAAAACTTTTATTGATTTATTTTTTGGGGGGAAATTTTTTAGGATCTGAT37290.5124047571608187No Hit
AACGTGGCCATCACAATAGATACGGTAGCAGATACATTGGACCACTGCTT36080.4957780541261019No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGTGTAGAAC35530.48822046183759416No Hit
GTGGAAACAGACTTCCTTTTGGCTAGGTGTTAAATTGTAGAACCATGTCG35020.4812125126246144No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG34790.4780520649403294No Hit
TGCCTGAATGTTCTTCACCACCTTCATGCAAACGTAAAGTTGGTGGTACA34510.4742045634116346No Hit
CAGCCATATCAGTCTGTGGGATCTGGTACAGTTTTGTAAATTCATCTGGA33680.46279946959443213No Hit
GCTGAAAGTCTGGATCTAAAAATTTGCTGAAGTGAAGGGGCTCCCGTGTA32710.44947062501288226No Hit
TGGTGGCAAACTCTCCTTCTCGTCTTGGTGGTACATCAGGGAGGTGTAGA32640.4485087496307085No Hit
TGCAATTTCTTGAACACTATCTCGCGGTAGCAGATACATTGGACCACTGC31230.4291338312183526No Hit
GCCTTTTGGCTAGGTGTTAAATTGTAGAACCATGTCGTCAGTGTAGATCT30890.424461865076366No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAC30220.41525534356127486No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG30020.4125071281836357No Hit
CAGCCATATCAGTCTGTGGGAAGAAGCTCTGCATCCTGTAGAACCATGTC29690.4079725728105311No Hit
ATGATCCATGGACATTTGACTGGTATTTCCACTAGAAGGAATATCACATC29250.401926498979725No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG27660.3800781867274938No Hit
TCCATCCATAATTCCACAAAGGAGGCAACGTTGAATGTAGAACCATGTCG26970.3705968436746388No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTTTATACTGCAAATGCTATC26590.3653752344571244No Hit
CCTGGTCCTCTGACTGCTCTAAAGTCCTTTTTCAAGTACATGTAGAACCA26410.36290184061724917No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC26320.36166514369731156No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT25480.35012263911122715No Hit
ATCTTACCTTCAACAGAATGACAGGATTTATGCAGATACATTGGACCACT25430.3494355852668174No Hit
TGCAATTTCTTGAACACTATCTCGAAATGAAGAGAAGATCATAACTCGGG25020.34380174374265715No Hit
AAGACGGTACAACTTCCTTGGAGATTTTGTCACTTCCACTCTCAAAGGGC24880.34187799297830973No Hit
GGTTGAACTAAAACGACAATACAATGTCGGTAGCAGATACATTGGACCAC23670.32525128994359287No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT23520.32319012841036354No Hit
TGGTGGCAAACTCTCCTTCTCCGGTAGCAGATACATTGGACCACTGCTTT23170.318380751499495No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA23010.31618217919738373No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT22630.3109605699798693No Hit
TGGTGGCAAACTCTCCTTCTCAACCAAAAAGAAAGCAATCAACTATTTCA22410.30793753306446625No Hit
GGTTGAACTAAAACGACAATACAATGTGGTAGCAGATACATTGGACCACT21930.3013418161581323No Hit
GCTGAAAGTCTGGATCTAAAAATTTGCTCGGTAGCAGATACATTGGACCA21450.29474609925179834No Hit
GCCTCTAGAAATCATGACTAGGTTTGTGCTCTGGAGGACTGTAGAACCAT21290.29254752694968705No Hit
GGTTGAACTAAAACGACAATACAATGTACGGGTAGCAGATACATTGGACC21210.2914482407986314No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC20430.2807302008258387No Hit
ATGATCCATGGACATTTGACTGGTATACGGTAGCAGATACATTGGACCAC20220.2778445746793176No Hit
GCAATTATGTTAGCTAGGAGCAGATACATTGGACCACTGCTTTTTGTAGA19510.26808841008869866No Hit
GGTTGAACTAAAACGACAATACAATGGTACGGTAGCAGATACATTGGACC19190.263691265484476No Hit
AACGTGGCCATCACAATAGAGCAGATACATTGGACCACTGCTTTTTGTAG18490.25407251166273903No Hit
CATGAGGGTAGTGTTGACAAAGATTAATACGGTAGCAGATACATTGGACC17960.2467897409119953No Hit
TTTCCAATTCCCTCCCAACCTACGGTAGCAGATACATTGGACCACTGCTT17760.24404152553435615No Hit
AACGTGGCCATCACAATAGATGTAGAACCATCGGTTTCCTTCGCACAAAA17750.24390411476547416No Hit
CATCTGGTTTTCAGGCACTTCAGTGTTTCCTGTAGAACCATGTCGTCAGT17420.23936955939236956No Hit
TCATTTGATGGGATAATAACAGTTCCAGAATGCCAGAGGCTGTGTAGAAC16920.2324990209482717No Hit
AAGACGGTACAACTTCCTTGGAGTAACGGTAGCAGATACATTGGACCACT15900.21848312252231208No Hit
ACTAACAAGCACTTATCAAAACTGAAAAATTACAATGAAAGGTTTGTACC15740.21628455022020074No Hit
CATCTGGTTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATGTCGTCAGT15430.2120248163848601No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG15040.20666579639846372No Hit
ATGATCCATGGACATTTGACTGGTATACGGAGCAGATACATTGGACCACT14650.20130677641206743No Hit
CCTGGTCCTCTGACTGCTCTGGAGTCTGGTCTCTGGTGTAGAACCATGTC14610.20075713333653958No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT14330.19690963180784476No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTGCAGATACATTGGA14060.19319954104803194No Hit
CGGTGGCAAACTCTCCTTCTCTACGGTAGCAGATACATTGGACCACTGCT13600.18687864567946189No Hit
CCTGGTCCTCTGACTGCTCTATGTGCTCAGCCAGTGTAGAACCATGTCGT13480.18522971645287842No Hit
CCTGGTCCTCTGACTGCTCTGAAGCAAAGGAGGCAACGTTGAATGTAGAA12810.17602319493778726No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT12540.17231310417797444No Hit
CCCCCTACTCCCTCTTATCCAAAAGTGAAACATTTTGTCCTTGTAGAACC12490.17162605033356465No Hit
AACGTGGCCATCACAATAGACTTTCCAGTCCCATTTCTAAGAGATGTAGA12230.16805337034263373No Hit
CCCCCTACTCCCTCTTACCCAAAAGTGAAACATTTTGTCCTTGTAGAACC12040.16544256573387656No Hit
GGAGAGCTGTAAATTCTGGCTTTTCCAGAATGCCAGAGGCTGTGTAGAAC10790.1482662196236319No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAA10630.14606764732152058No Hit
AAGACGGTACAACTTCCTTGGAGATTTTGTCACTTCCACTTTCAAAGGGC10580.1453805934771108No Hit
ACTAGATATTCCTTGTCATTATCTGCACCATTGAGCAAACTGGTTGTAGA10560.14510577193934687No Hit
CAGCCATATCAGTCTGTGGGAGCCAGCCTTCTAACTGTAGAACCATGTCG10520.14455612886381905No Hit
TAAGGAGAATGATTTGTAGTGGCCAAAATTGAATGCTATGCTTAGATGTA10450.14359425348164537No Hit
GCTGAAAGTCTGGATCTAAAAATTTGCTACCGTAACAATGAAAGGTTTGT10430.14331943194388144No Hit
AACATGTTCCAGAGCAGTTGGTAGGTAAGTGGAGTGAAATTTAATGTAGA10320.1418079134861799No Hit
CCTGGTCCTCTGACTGCTCTTCATCAACACAAAAACTTGTAGAACCATGT10090.1386474658018949No Hit
TGTACCATGTACTAAAAAATTACATTTGGAAAAAGAAAATCAGCCTGCAC9690.1331510350466166No Hit
ACTAGATATTCCTTGTCATTATCTACACCATTGAGCAAACTGGTTGTAGA9620.13218915966444292No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA9260.12724237198469243No Hit
CCTGGTCCTCTGACTCCTCTTCATCAACACAAAAACTTGTAGAACCATGT9110.12518121045146308No Hit
CGCAATTTCTTGAACACTATCTCGTACGGTAGCAGATACATTGGACCACT8990.12353228122487958No Hit
TGTACCATGTACTAAAAAATTACATTTGGAAAAATACGGTAGCAGATACA8680.11927254738953892No Hit
CCTGGTCCTCTGACTGCTCTTACGGTAGCAGATACATTGGACCACTGCTT8490.11666174278078174No Hit
AGATTAGGGGTTTTGCAACCTGAAAGTGTTAATGCTAACGATGTAGAACC8460.11624951047413586No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTATCATGAA8000.10992861510556583No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)