FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345442
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTGTGTGTGTGGCTTCAAAAAAATTGTTGGCTGTTTCTCTTTTCTCCG95772.7723901552214265No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC81932.3717440264935936No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA71502.069812008962431No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA69982.025810411009663No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC66741.9320175311629737No Hit
GGATCCTGGGTGTTTGTATTTGCCTTAGAGGTGAGTCATTCAGTAATCAC61971.793933569166459No Hit
GCCCTGCACAGAATTTAGTTGGAACACTGACTTATGGTATCAAATAATAA61141.7699063808106714No Hit
TTCTGATGAGCCAAGTCTCTGTGGGAGGAGGGCAGTACACAGACCAAGTC59881.7334313719814036No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC58321.688271837240405No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT57631.668297427643425No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT55491.606347809473081No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCACCACCAGTTTGTACTCAGTCAT55241.5991107045466388No Hit
AGCAATCTGCTTGTGTGTGCGTGAGACCAATTCTGATGAGCCAAGTCTCT50941.4746324998118354No Hit
AGTGGAACTGTTATTATCCCTCAAATGGCACAGCTGAGACCAAGTCTCTG49141.4225253443414525No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC47681.3802606515710307No Hit
CCAAATCTGCTTTCTTGATAAAATCCTTTGAATGGCATGCAGACCAAGTC44981.3020999183654565No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC44211.279809635192015No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA41261.1944117970599986No Hit
AGTGGAACTGTTATTATCCCATCAAATGGCACAGCTGAGACCAAGTCTCT41201.1926748918776524No Hit
TGAGGAATGTTTGATTTTTAAGTACTAGCACTGTACGGTCCTTGCAAGAC35621.0311427099194657No Hit
TCAAAGGGCTCCACTGGTTTTTCTTATCTCTTTCATTTGATTAGACCAAG35541.0288268363430042No Hit
GGGGTCAGAGGCAAGCAGATATCCGGTGTAGACCAAGTCTCTGCTACCGT33610.9729563863108713No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT33410.9671667023697176No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC30230.8751107277053746No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA29640.8580311600789713No Hit
GGGCAGTTGGCCACTTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTG27780.8041870994262423No Hit
GAAAGTGGTGCATTGATGAAGGCCTGATGTAGGTCTCAGACCAAGTCTCT27770.8038976152291846No Hit
GGGGACGCTCTTGTATTATCTGTACACTGACTTATGGTATCAAATAATAA27640.8001343206674347No Hit
GAAAGTGGTGCATTGATGGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC27580.7983974154850887No Hit
CCTCAGGGCCTCAGAGAAAGGGTGGGGAAACTTGAAAGTAGACCAAGTCT27040.7827652688439738No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTATTG26420.7648172486263976No Hit
TCAAAGGGCTCCACAGTTTTTCTTATCTCTTTCATTTGATTAGACCAAGT26240.7596065330793592No Hit
CTGACACTTACAACAGTCAAGTTCTCCTCAGATGACTCCATTTAAAGACC26020.7532378807440901No Hit
CAAAGTGGGAAGGGGAGTGAATATCGAATCATGGAATCCCAGACCAAGTC25540.7393426392853214No Hit
GGTGCCAGTTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACCG25270.731526565964764No Hit
GGGCAGTTGGCCACTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTGC25000.7237104926442066No Hit
CAAAGTGGGAAGGGGAGTGAACATCGAATCATGGAATCCCAGACCAAGTC24640.7132890615501299No Hit
AATGGTTTTACCAAGGAAGAATCCCCACCACAATACCAAGACCAAGTCTC23380.6768140527208619No Hit
AGTTCCATTTAGGTTGCTGGTGAAGAACATTCAGGCAAGACCAAGTCTCT22940.6640767480503239No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTATTGTCGA22910.6632082954591509No Hit
GTTGTTTCAGAACACCGAAAGCTCAAGAAGCATGTCATAGACCAAGTCTC22220.6432338858621708No Hit
GGGGTCAGAGGAAGCAGATATCCGGTGTAGACCAAGTCTCTGCTACCGTA21670.6273122550239982No Hit
GTTGTTTCAGAACACCGAAAAGCTCAAGAAGCATGTCATAGACCAAGTCT21280.6160223713387486No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACAAAATGAAATA21190.6134170135652295No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTATT20810.6024166140770375No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT20440.5917056987859033No Hit
TGCAGGGCTGATTGTCTTCTTTACACTGACGACATGGTTCTACCTTTTTC19750.5717312891889232No Hit
GATTCCCGCGCCCAGAGTCCCACGACCCCCTCAGACCAAGTCTCTGCTAC19600.5673890262330579No Hit
AATGGTTTTACCAAGGAAGGATCCCCACCACAATACCAAGACCAAGTCTC19420.5621783106860196No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTATTGT19330.5595729529125005No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAT19040.5511779111978277No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC18770.5433618378772703No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT18370.531782469994963No Hit
CATCTCCATGAGTAGGAAGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC18240.5280191754332131No Hit
TGTAGTTTCTAAGGGTCGAATTCTTTAAGTTCACTGGTATTTGAGACCAA18170.5259927860538093No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC17690.5120975445950405No Hit
TCTCCCCCACCCCTTCTACTCACTCAAAGACATCTGCAGACCAAGTCTCT17480.5060183764568292No Hit
AGTTCCATTTAGGTTGCTGGTGGAGAACATTCAGGCAAGACCAAGTCTCT17430.5045709554715407No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAAA17080.4944390085745219No Hit
GAGATTGATGACCAAAAGAACTGCTACCGTAAGCTATAAGTATCTTCTCT16660.48228067229809923No Hit
GAGTTTGATGCACAACTTCAAGGCACACTGACTTATGGTATCAAATAATA16070.46520110467169595No Hit
CCGGTACAAACCTTTCATTGTATTGTCGTTTTAGTTCAACCAGACCAAGT15640.4527532841982156No Hit
GCTTTCACTTCTCTGGCTGAACACACAGCTCAGAATACTAGTAGACCAAG15570.45072689481881184No Hit
CCAAATCTGCTTTCTTGATAAAATCCTCTGAATGGCATGCAGACCAAGTC15490.4484110212423504No Hit
CTCATTCTTTCCTTGATTTTCTTCCTTACGGTAGCAAATACTGAGCATCA14210.411357044018967No Hit
TTGGACTCAATTTCAACTCTAACTTTAAATGCAAACTAGGCCAGGCAGAC13910.40267251810723653No Hit
TGAACTTATATATTTGACACAACCCAGCAACCCATTCAGCAGAATTAGAC12600.3647500882926801No Hit
GAAGTATTACAAATGGAAGATGATCTGGTGCACTTTTCTGAGGACCATAA12280.35548659398683424No Hit
CATCTCCATGAGTAGGATGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC12070.34940742584862294No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTATTGTCG11810.34188083672512315No Hit
GGAATCCTATGGCTTTCCAACCTACGGTAGCAGAAAGTCAGTCCCATGGA11300.3271171426751814No Hit
CAGCAATGTGGCTTTAAGTAGAACATATCATCTGGACCCTTTTCAGACCA11240.3253802374928353No Hit
TCTGGGGTATCAGGTAGGTGTCTACGGTAGCAGAAAGTCAGTCCCATGGA10570.30598479628997055No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA10550.3054058278958552No Hit
TGAATGTAGCACGCATTCACATAGGTTAACATTTACCACCCCTAGACCAA10190.29498439680177857No Hit
AACAATCGACAGGCTAGAAGTTGAGACCAAGTCTCTGCTACCGTATTGTC10060.2912211022400287No Hit
GGAAACACTGATGGGCCTGAAAGTCACACCACCTCAGCAACCAGACCAAG9620.2784837975694907No Hit
TGACCAATGGCTAAGTGAAGATGAACACTGACCCATGTGGAATAGTTCCC9460.27385205041656774No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTATTGTCGAGACATC9410.2724046294312793No Hit
GGCTTCCACTTAATTCCAGTCTAAAAAGTTACCCAGAAGTGTAGACCAAG9370.2712466926430486No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC9220.26690442968718336No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG9200.266325461293068No Hit
TGGCTTATAAAATATTAATGTGCTTCTGTTTCAAAAGACCAAGTCTCTGC9180.26574649289895264No Hit
AAGGGAAAAGGATGGATTAGGGTACACTGACGACATGGTTCTACTACCCT8890.2573514511842798No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAAAAATGAAATACTTTGAG8620.24953537786372243No Hit
CAGCAATGTGGCTTTAATTAGAACATATCATCTGGACCCTTTTCAGACCA8580.24837744107549167No Hit
CTACTAATGCCCACAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTCT8510.2463510516960879No Hit
TCTCCCCCACCCCCTCTACTCACTCAAAGACATCTGCAGACCAAGTCTCT8320.24085085195199193No Hit
ACTTTCCTCTGGCAATTGGACATACGACATGGTTCTACATTCCTGCCATC8220.2379560099814151No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTATTGT8190.23708755739024207No Hit
TGCATTCTGAAAAAGCCAGAATTTACAGCTCTCCAGACCAAGTCTCTGCT8080.23390323122260756No Hit
AAGTCCTCCATTTCTGTATCCATGCGAGACCAAGTCTCTGCTACCGTATT7630.22087644235501183No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA7430.21508675841385821No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG7390.21392882162562746No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT7280.21074449545799295No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTATTG7180.2078496534874161No Hit
GCCTGCTGAAAATGCCAGAACCCCTCAGATGTTAGACCAAGTCTCTGCTA7130.20640223250212772No Hit
GCCTGCTGAAAATGACTGAACCCCTCAGATGTTAGACCAAGTCTCTGCTA6990.20234945374332014No Hit
AGAAAACGGCATTTTGAGTGTACGGTAGCACAGCAAGTGGAAAATCTGTA6970.20177048534920478No Hit
TTTCTCCTCCCTGTGGTTCTACTGGGAGGAGGGCAGTACACAGACCAAGT6810.1971387381962819No Hit
CACATCCTCCTAAACACACAGATGACGATTCCTTCTGTGTTTAGACCAAG6780.19627028560510884No Hit
CACTTAATGAGACCAACAGTAACACCTTTATCACTCACAAGACCAAGTCT6640.19221750684630126No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG6230.18034865476693626No Hit
CTACTAATGCCCACAAAGAGATACTTTCCTGAGTGCCAGACCAAGTCTCT6200.17948020217576321No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACAAGGTAATTAAATGTGGTAC6200.17948020217576321No Hit
TGCTAATTGGGCAGATAGAGCTTCGTTCAGACCAAGTCTCTGCTACCGTA6040.1748484550228403No Hit
TGATAAATGCACCTGTTTGCTGTCACATTCAGAGACCAAGTCTCTGCTAC5980.1731115498404942No Hit
AGTAAGGGTGGCATCAAAAATTGGTTTTCTTTGCAGACCAAGTCTCTGCT5940.17195361305226348No Hit
GCCAAACGAAAATTATGGCAGGTACACTGACGACATTCAGAGTGAAGAAA5820.16847980268757126No Hit
CACATCCTCCTAAACACACAGGTGACGATTCCTTCTGTGTTTAGACCAAG5720.16558496071699447No Hit
GATGTGCCACCATACCCAGAGACCAAGTCTCTGCTACCGTATTGTCGAGA5650.1635585713375907No Hit
GGATGAGAACTCCGAGAAGCAGCATATAAAAATGACTCTAGGTAGACCAA5640.163269087140533No Hit
GCTTTCACTTCTCTGGCTGAGCACACAGCTCAGAATACTAGTAGACCAAG5620.16269011874641764No Hit
TTCTGATGAGCCAAGTCTCTGTGAAACAGTAGGTATGTAAAATTTAAGTC5530.16008476097289848No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTATTGTC5420.15690043480526397No Hit
AGCACCAAACGTAAAGCAGCAACAGAGGAGGGGGAGACCAAGTCTCTGCT5410.1566109506082063No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTATTGTC5180.1499528140758796No Hit
TATTTCTGGACAGTCTACGAAAAGCTCTACACTGACGACATGGTGGTTGA5090.14734745630236046No Hit
TGGAGCCACATAACACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTC5080.14705797210530278No Hit
AAGGGAAAAGGATGGATTAGGGTACACTGACGACATGGTTCTACAAAGGG5000.14474209852884132No Hit
GGCTTCCACTTAATTCCAGTCTGAAAAGTTACCCAGAAGTGTAGACCAAG4890.14155777236120679No Hit
TCTTGCTTTTTTATTTCAGGATGCTTACGGTAGCAGGTCTTGGCCAGTTG4860.14068931977003377No Hit
TTTTTGTCCAGCCAGCAAATGATCAGCCTTGCAAAGACCAAGTCTCTGCT4790.13866293039063No Hit
TGAGCAAGAGGCTTTGGAGGAAAAGAGAAGCAAGAGGCAGACCAAGTCTC4740.13721550940534155No Hit
TGAATGTAGCACGCATTCACATAAGTTAACATTTACCACCCCTAGACCAA4680.13547860422299549No Hit
GCCACACCTCACGTTAGAATTTTCCAGTGAAGACCAAGTCTCTGCTACCG4570.13229427805536095No Hit
GCTATTCCTGTCATTCTGTTGAAGGTAAGATAGACCAAGTCTCTGCTACC4510.13055737287301486No Hit
TGCAGATTTCATGTGCCTAGAAGTTACGGTAGCAAAGGCCAATCAAGTGA4440.12853098349361108No Hit
TCTAAGCATAGCATTCAATTTTGGCGCTGGAATCCGAATTAGACCAAGTC4310.12476768893186121No Hit
AGCAATCTGCTTGTGTGTGCGTGAGGCCAATTCTGATGAGCCAAGTCTCT4280.12389923634068815No Hit
GTACAATTTCTACCGCTGGTTCCACATGAAAAAGAGCAAGGTACTAGTGA4150.12013594177893828No Hit
TTCAGGAAGAAGAACGATTATCCAGAAAGACCAAGTCTCTGCTACCGTAT4100.11868852079364987No Hit
AATGAAGAACTTGCTAGATAATCATACTGGCCATTCTTTACTGCACAAGA3960.11463574203484231No Hit
ATTTAACACCTAGCCAAAAGGCAGACCAAGTCTCTGCTACCGTATTGTCG3950.11434625783778463No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTATT3830.11087244747309244No Hit
TGTCATAATGAATGCCCCATCGTGTAGAACCATACCCTCAATTTGGAAGC3780.10942502648780403No Hit
GACTACAGATGTGCCACCATACCAACTGCTCTGGAACATGTTAGACCAAG3750.10855657389663097No Hit
AGGACGAAAACTGGACACTCTCAGCAGGCAAAGACCAGACCAAGTCTCTG3730.10797760550251563No Hit
AGGACGAAAACTGGACATTCTCAGCAGGCAAAGACCAGACCAAGTCTCTG3710.10739863710840025No Hit
ATCTCCTGGTTATTCTCAGTTTTCTTTACCAGTGCAAGACCAAGTCTCTG3630.10508276353193878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)