FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345442
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG105323.0488475634115133No Hit
GTAAAACCCACTAATACTTGAAGGTCATTCTCTTTTTTACCTTGTAATAC101782.9463701576530936No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG79322.2961886510615384No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT70702.046653273197816No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT69212.0035201278362216No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA66431.9230435210541856No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTTCCAACTAAATTCT60361.7473266134401724No Hit
GTGTACTGCCCTCCTCCCACAGAGACTTGGCTCATCAGAATGTAGAACCA59681.72764168804025No Hit
TTGTGATTACTGAATGACTCACCTCTAAGGCAAATACAAACACCCAGGAT59091.7105621204138464No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT57311.6590339333375792No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC55001.5921630838172545No Hit
CACAGAGACTTGGCTCATCAGAATTGGTCTCACGCACACACAAGCAGATT50871.4726061104324315No Hit
CAGCTGTGCCATTTGAGGGATAATAACAGTTCCACTTGTAGAACCATGTC48811.412972365838549No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG47231.367233862703435No Hit
GCATGCCATTCAAAGGATTTTATCAAGAAAGCAGATTTGGTGTAGAACCA44931.300652497380168No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT41621.204833228154075No Hit
CAGCTGTGCCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGT40911.1842798501629797No Hit
TGCAAGGACCGTACAGTGCTAGTACTTAAAAATCAAACATTCCTCATGTA35471.0268004469636003No Hit
AATCAAATGAAAGAGATAAGAAAAACCAGTGGAGCCCTTTGATGTAGAAC35291.021589731416562No Hit
ACACCGGATATCTGCTTGCCTCTGACCCCTGTAGAACCATGTCGTCAGTG33510.9700615443402943No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT33110.9584821764579872No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG30030.8693210437642209No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT29430.85195199194076No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTGCAGAGACTTGGTCTGTCTAAGA28350.8206876986585303No Hit
GAGACCTACATCAGGCCTTCATCAATGCACCACTTTCTGTAGAACCATGT27590.7986868996821463No Hit
CTGCACCCAGCCATAAGTAAAAGTGGCCAACTGCCCTGTAGAACCATGTC27540.797239478696858No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTACAGATAATACAAG27540.797239478696858No Hit
GAGACCTACATCAGGCCTTCCATCAATGCACCACTTTCTGTAGAACCATG27440.794344636726281No Hit
ACTTTCAAGTTTCCCCACCCTTTCTCTGAGGCCCTGAGGTGTAGAACCAT26650.7714753851587242No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA26270.7604749856705322No Hit
AATCAAATGAAAGAGATAAGAAAAACTGTGGAGCCCTTTGATGTAGAACC26030.7535273649411479No Hit
TTAAATGGAGTCATCTGAGGAGAACTTGACTGTTGTAAGTGTCAGTGTAG25610.7413690286647252No Hit
GAGTCCTCCTTCTGTGAGCAAACTGGCACCTGTAGAACCATGTCGTCAGT25530.7390531550882637No Hit
GGGATTCCATGATTCGATATTCACTCCCCTTCCCACTTTGTGTAGAACCA25300.732395018555937No Hit
CTGCACCCAGCCATAAGTAAAGTGGCCAACTGCCCTGTAGAACCATGTCG24860.719657713885399No Hit
GGGATTCCATGATTCGATGTTCACTCCCCTTCCCACTTTGTGTAGAACCA24370.7054729882295725No Hit
TGGTATTGTGGTGGGGATTCTTCCTTGGTAAAACCATTTGTAGAACCATG22990.6655241690356123No Hit
TGCCTGAATGTTCTTCACCAGCAACCTAAATGGAACTTGTAGAACCATGT22840.6611819060797471No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC22660.6559711905327088No Hit
ATGACATGCTTCTTGAGCTTTCGGTGTTCTGAAACAACTGTAGAACCATG22080.6391811071033632No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTGTCTGTCTAAGAACACAGAGGAG21650.6267332866298829No Hit
ACACCGGATATCTGCTTCCTCTGACCCCTGTAGAACCATGTCGTCAGTGT21620.6258648340387098No Hit
ATGACATGCTTCTTGAGCTTTTCGGTGTTCTGAAACAACTGTAGAACCAT21090.6105221715946526No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG21080.610232687397595No Hit
TTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT20170.5838896254653458No Hit
GAGGGGGTCGTGGGACTCTGGGCGCGGGAATCTGTAGAACCATGTCGTCA19390.5613098580948466No Hit
TGGTATTGTGGTGGGGATCCTTCCTTGGTAAAACCATTTGTAGAACCATG19320.5592834687154429No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT19230.5566781109419237No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA19120.5534937847742891No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG18630.5393090591184627No Hit
ACTGCCCAACCAGAAAAAGGTAGAACCATGTCGTCAGTGTAAAGAAGACA18390.5323614383890783No Hit
CAAATACCAGTGAACTTAAAGAATTCGACCCTTAGAAACTACATGTAGAA18210.52715072284204No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT18060.5228084598861749No Hit
GAGACCTACATCAGGCCTTCTTCCTACTCATGGAGATGTGTAGAACCATG18050.5225189756891171No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG17660.5112290920038675No Hit
GCAGATGTCTTTGAGTGAGTAGAAGGGGTGGGGGAGATGTAGAACCATGT17490.5063078606538869No Hit
TGCCTGAATGTTCTCCACCAGCAACCTAAATGGAACTTGTAGAACCATGT17410.5039919870774254No Hit
CCATGGACAAGAGAAGATACTTATAGCTTACGGTAGCAGTTCTTTTGGTC16560.4793858303275224No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG16040.46433265208052293No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTGCCTTGAAGTTGTG15950.46172729430700377No Hit
CCAAAGCAGAAAGGGTAGTAGAACCATGTCGTCAGTGTACCCTAATCCAT15900.4602798733217154No Hit
GGTTGAACTAAAACGACAATACAATGAAAGGTTTGTACCGGTGTAGAACC15730.45535864197173476No Hit
ACTAGTATTCTGAGCTGTGTGTTCAGCCAGAGAAGTGAAAGCTGTAGAAC15570.45072689481881184No Hit
GCATGCCATTCAGAGGATTTTATCAAGAAAGCAGATTTGGTGTAGAACCA15370.44493721087765814No Hit
GCCTGGCCTAGTTTGCATTTAAAGTTAGAGTTGAAATTGAGTCCAATGTA13860.4012250971219481No Hit
AGTGAACTTGATGCTCAGTATTTGCTACCGTAAGGAAGAAAATCAAGGAA13620.3942774763925638No Hit
AATTCTGCTGAATGGGTTGCTGGGTTGTGTCAAATATATAAGTTCATGTA12480.36127627792798794No Hit
TATGGTCCTCAGAAAAGTGCACCAGATCATCTTCCATTTGTAATACTTCT12280.35548659398683424No Hit
GAGACCTACATCAGGCCTTCATCCTACTCATGGAGATGTGTAGAACCATG11910.3447756786957No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT11780.34101238413395013No Hit
CCATGGGACTGACTTTCTGCTACCGTAGGTTGGAAAGCCATAGGATTCCT11240.3253802374928353No Hit
GAAAAGGGTCCAGATGATATGTTCTACTTAAAGCCACATTGCTGTGTAGA11200.32422230070460456No Hit
CCATGGGACTGACTTTCTGCTACCGTAGACACCTACCTGATACCCCAGAT10560.30569531209291284No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT10440.30222150172822065No Hit
AGGGGTGGTAAATGTTAACCTATGTGAATGCGTGCTACATTCATGTAGAA10130.2932474916194325No Hit
CAACTTCTAGCCTGTCGATTGTTTGTAGAACCATGTCGTCAGTGTAGATC9910.28687883928416347No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT9620.2784837975694907No Hit
GGTTGCTGAGGTGGTGTGACTTTCAGGCCCATCAGTGTTTCCTGTAGAAC9570.27703637658420227No Hit
GGGAACTATTCCACATGGGTCAGTGTTCATCTTCACTTAGCCATTGGTCA9380.27153617684010634No Hit
ACACTTCTGGGTAACTTTTTAGACTGGAATTAAGTGGAAGCCTGTAGAAC9340.2703782400518756No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG9130.2642990719136642No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT9110.26372010351954883No Hit
GCAGATGTCTTTGAGTGAGTAGAGGGGGTGGGGGAGATGTAGAACCATGT9090.26314113512543347No Hit
TTTGAAACAGAAGCACATTAATATTTTATAAGCCATGTAGAACCATGTCG8990.2602462931548567No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTTAAGAACACAGAGGAGAATTTAT8780.25416712501664535No Hit
GAAAAGGGTCCAGATGATATGTTCTAATTAAAGCCACATTGCTGTGTAGA8570.248087956878434No Hit
GGCACTCAGGAAAGTATCTCGTTGTGGGCATTAGTAGTGTAGAACCATGT8390.24287724133139574No Hit
GGAGAGCTGTAAATTCTGGCTTTTTCAGAATGCATGTAGAACCATGTCGT8150.2359296206020113No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTATGTCCAATTGC8120.23506116801083832No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT8110.23477168381378063No Hit
CGCATGGATACAGAAATGGAGGACTTTGTAGAACCATGTCGTCAGTGTAG7600.22000798976383878No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA7380.21363933742856978No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC7350.21277088483739673No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT7260.21016552706387756No Hit
AACATCTGAGGGGTTCTGGCATTTTCAGCAGGCTGTAGAACCATGTCGTC7130.20640223250212772No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA7090.205244295713897No Hit
ACAGATTTTCCACTTGCTGTGCTACCGTACACTCAAAATGCCGTTTTCTT6920.20032306436391636No Hit
AACATCTGAGGGGTTCAGTCATTTTCAGCAGGCTGTAGAACCATGTCGTC6890.19945461177274332No Hit
GTGTACTGCCCTCCTCCCAGTAGAACCACAGGGAGGAGAAATGTAGAACC6740.19511234881687808No Hit
AAACACAGAAGGAATCGTCATCTGTGTGTTTAGGAGGATGTGTGTAGAAC6730.1948228646198204No Hit
TGTGAGTGATAAAGGTGTTACTGTTGGTCTCATTAAGTGTGTAGAACCAT6680.193375443634532No Hit
GCAAAGAAAACCAATTTTTGATGCCACCCTTACTTGTAGAACCATGTCGT6300.18237504414634004No Hit
GGCACTCAGGAAAGTATCTCTTTGTGGGCATTAGTAGTGTAGAACCATGT6210.1797696863728209No Hit
GAACGAAGCTCTATCTGCCCAATTAGCATGTAGAACCATGTCGTCAGTGT6160.17832226538753249No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT6160.17832226538753249No Hit
GTTGTAAAATTTCTTCACTCTGAATGTCGTCAGTGTACCTGCCATAATTT5880.17021670786991738No Hit
TGCACGTACCACATTTAATTACCTTGTCTTCTCTTGGTATTAGTGCTTGA5850.16934825527874434No Hit
CTGAATGTGACAGCAAACAGGTGCATTTATCATGTAGAACCATGTCGTCA5840.16905877108168665No Hit
AAACACAGAAGGAATCGTCACCTGTGTGTTTAGGAGGATGTGTGTAGAAC5720.16558496071699447No Hit
CTGGGTATGGTGGCACATCTGTAGAACCATGTCGTCAGTGTAGATCTCGG5590.1618216661552446No Hit
ACTAGTATTCTGAGCTGTGTGCTCAGCCAGAGAAGTGAAAGCTGTAGAAC5580.16153218195818692No Hit
ACCTAGAGTCATTTTTATATGCTGCTTCTCGGAGTTCTCATCCTGTAGAA5550.16066372936701384No Hit
CCCCCTCCTCTGTTGCTGCTTTACGTTTGGTGCTTGTAGAACCATGTCGT5390.15603198221409093No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC5380.15574249801703324No Hit
AAGACTTAAATTTTACATACCTACTGTTTCACAGAGACTTGGCTCATCAG5250.15197920345528337No Hit
ATGTTACCTTAAGATCAACCACCATGTCGTCAGTGTAGAGCTTTTCGTAG5230.151400235061168No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC5140.14879487728764887No Hit
TTCTGGATAATTTCTTTTTTGAATGTGTTATGTGGCTCCATGTAGAACCA4980.14416313013472595No Hit
CCAACTGGCCAAGACCTGCTACCGTAAGCATCCTGAAATAAAAAAGCAAG4900.14184725655826447No Hit
ACACTTCTGGGTAACTTTTCAGACTGGAATTAAGTGGAAGCCTGTAGAAC4880.1412682881641491No Hit
TTGCAAGGCTGATCATTTGCTGGCTGGACAAAAATGTAGAACCATGTCGT4770.1380839619965146No Hit
GCCTCTTGCTTCTCTTTTCCTCCAAAGCCTCTTGCTCATGTAGAACCATG4700.13605757261711085No Hit
AGGGGTGGTAAATGTTAACTTATGTGAATGCGTGCTACATTCATGTAGAA4600.133162730646534No Hit
TCACTGGAAAATTCTAACGTGAGGTGTGGCTGTAGAACCATGTCGTCAGT4480.1296889202818418No Hit
ATCTTACCTTCAACAGAATGACAGGAATAGCTGTAGAACCATGTCGTCAG4410.12766253090243804No Hit
CACAGAGACTTGGCTCATCAGAATTGGCCTCACGCACACACAAGCAGATT4310.12476768893186121No Hit
CTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG4270.12360975214363047No Hit
AATTCGGATTCCAGCGCCAAAATTGAATGCTATGCTTAGATGTAGAACCA4250.12303078374951511No Hit
TTCTTCACTTGATTGGCCTTTGCTACCGTAACTTCTAGGCACATGAAATC4230.12245181535539974No Hit
CACTAGTACCTTGCTCTTTTTCATGTGGAACCAGCGGTAGAAATTGTACT4110.11897800499070756No Hit
TTCTGGATAATCGTTCTTCTTCCTGAATGTAGAACCATGTCGTCAGTGTA4050.11724109980836148No Hit
TGTGCAGTAAAGAATGGCCAGTATGATTATCTAGCAAGTTCTTCATTTGT4020.11637264721718842No Hit
GCCTTTTGGCTAGGTGTTAAATTGTAGAACCATGTCGTCAGTGTAGATCT4010.11608316302013073No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG3800.11000399488191939No Hit
GGTCTTTGCCTGCTGAGAGTGTCCAGTTTTCGTCCTTGTAGAACCATGTC3770.10913554229074635No Hit
AACATGTTCCAGAGCAGTTGGTATGGTGGCACATCTGTAGTCTGTAGAAC3730.10797760550251563No Hit
GGTCTTTGCCTGCTGAGAATGTCCAGTTTTCGTCCTTGTAGAACCATGTC3670.10624070032016952No Hit
TGCACTGGTAAAGAAAACTGAGAATAACCAGGAGATTGTAGAACCATGTC3620.1047932793348811No Hit
AAGAGGCTTCCAAATTGAGGGTATGGTTCTACACGATGGGGCATTCATTA3610.10450379513782342No Hit
ACATGTTAGCTGACTGATGATGGTAGGCTGTAGAACCATGTCGTCAGTGT3600.10421431094076573No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)