FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521469
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT141282.709269390893802No Hit
CAACTGTCAGTCTGCCATTCTTTAAGACAGCTAAGAGGGGAGGATCTAAC135782.6037981164748047No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT130412.500819799451166No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG120132.3036843992643856No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT108162.0741405529379504No Hit
CAATGATGAACACTGCGGGCTTCCAGACAATGTTAACACGCTGAATGCCA107042.0526627661471726No Hit
ACACTTCTGGGTAACTTTTTAGACTCCAAACCTGAATCCTTAGCCTCTAC104832.0102824904260848No Hit
GTTGTAAAATTTCTTCACTCTGAATGTCAGCAAAAACCTTATGTGAATGC104522.004337745867923No Hit
CAGCTGTGCCATTTGAGGGATGCTCAGGTCGGTTTGGCTCTGCAGCAGGT101021.937219662146743No Hit
AAAGAGAGTGAGTCGTTGCGAAGCCCTGTGTTTCAGCTCATTGTGATTAA95401.8294471962858772No Hit
CAGTACTTGTCCCTGAGGTAGTTTCTTTTCGAGAAGTTTTGGATGGAGGT89931.7245512197273474No Hit
TGCAATTTGGTCTCTGCATGTACCACAGAATTGCTGCTTCCCTCAGCCTT85811.645543646889844No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC83271.596835094703616No Hit
TATGGTCCTCAGAAAAGTGCACCAAACCAGATTCTAGCAGTCCCATTAAG77061.477748437586894No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT69311.3291298236328526No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTGCTTGATTTTCTTACTTGGTCCAA69141.325869802423538No Hit
CCTGTCATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTA69111.3252945045630709No Hit
CAGACAGGGACAAATCATCAGCTGGAGACACCAGGTCTGCCTGAGAAGCC68641.316281504749084No Hit
TGCCTGAATGTTCTTCACCACCTTCATGCAAACGTAAAGTTGGTGGTACA67041.2855989521908302No Hit
AAAAGCCCCTAAACCCCACTTCATTTTCATCTGTTAAATAACCAGAAGCA65591.2577928889349128No Hit
AGCTTCATCATACCTTTCACTAAGACAAGAATTTAGAAGTGGACAGGAAA64291.2328633149813317No Hit
GCACCTGAGCTCAATGAGATTCATGACCGGTCTGTTTACCTGCTGCCTCC62981.2077419750742613No Hit
AGAGACATCTTAAAGAGGGAAGCTGTATTTTTCCAGAGGAACTGAGTCCT62251.193743060469558No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACGATATCACC62121.1912501030742No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA61561.1805112096788113No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG60941.1686217205624878No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA56641.086162360562181No Hit
TCTAAAGTATGCAGGTTTTGTTCAAGAAGTGCGGTACCTGCTGAATACAA54971.0541374463295037No Hit
GCAGAATTCCGAAGTTCAGCTGCATGCTGGTTTCCTTCACGACAGGTGTG54591.0468503400969185No Hit
ATCACAATATCTCATTTTGCCCTCTTTGCAACCAGTGCACATTACTGGGT51650.9904711497711274No Hit
TGTGGTTTAGCCCTCGAAGTAAGAAAGTCAGATATGTTGTGAGTAAAGCT50620.9707192565617515No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACCTCCATGATCTCCAACAAAG49950.9578709376779827No Hit
AGAACATTTCCTCAGAATTGTCCCAAAAGAGCTAGTTAAGGACAAAGTTG49940.9576791717244937No Hit
CACTCAAGAGCACATGTACAGCTGTATCTGATCAGTTCTAGCTGAGCCAG48620.9323660658639343No Hit
GGTCTTTGTGCCCAGACTTCAGCTCCCGTTCTTTCCTGGTCTTCTCCTTG48440.9289142787011309No Hit
CAAAAATAACTTGGAAAACGATGATAGGTTTTTATGTCACAAGTAGGCAT47820.9170247895848076No Hit
GGTTGAACTAAAACGACAATACAATGAAAGGTTTGTACCGGTGTAGAACC47390.9087788535847768No Hit
TGGGAGGTGAGAGTTTAATAAAATAGTCAAGGACACATAGCATTAACTGA45320.8690833012125362No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC44190.8474137484682694No Hit
AGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGG44110.8458796208403568No Hit
GAAAACTTTTATTGATTTATTTTTTGGGGGGAAATTTTTTAGGATCTGAT43710.8382089827007934No Hit
AAGACATCACTGAAATCATAGAACCCTTACGTTCACTGACTGGACCCATG42820.8211418128402648No Hit
TTAAATGGAGTCATCTGAGGAGAATTCAGTTCTTTTTTCTTTATGGGTGT41590.7975546005611073No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA40070.7684061756307661No Hit
CACTCAAGAGCACATGTACAGCTGTACCTGATCAGTTCTAGCTGAGCCAG36880.7072328364677478No Hit
GTGTCCTGCTTATTTTTCTCACATTCTTCCGTACTGGCCTGGGAACTCTC35900.6884397730258175No Hit
GCTGTCTACCTGACCAATCGATGGGGCATTCATTATGACATGAAGATCAG35060.6723314329327342No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTGCCTTGAAGTTGTG33900.6500865823280003No Hit
ATGATCCATGGACATTTGACTGGTATTTCCACTAGAAGGAATATCACATC32800.628992327444201No Hit
TAGAGGAGAGACTTTGCTCCATATTGCTTCTATTAAGGTAGGATGCTTAC32140.6163357745139213No Hit
ACTTTCAAGTTTCCCCACCCTTTCTCTGAGGCCCTGAGGTGTAGAACCAT28190.5405882228857325No Hit
GATGTTTTGAGATTTTCAGTTTGTCTACATAAATTATCACTTAAGAGCTT27510.5275481380484747No Hit
GAGGGGGTCGTGGGACTCTGGGCGCGGGAATCTGTAGAACCATGTCGTCA26930.5164257127461077No Hit
CCCCATGTCCAGGCTCATGGATTTCCTGGCTCGGGGACTGGTCTGGCCGA25920.49705735144370994No Hit
GTGTACTGCCCTCCTCCCACAGAGACTTGGCTCATCAGAATGTAGAACCA25890.4964820535832427No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG25490.4888114154436793No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC25440.4878525856762339No Hit
AACATCTGAGGGGTTCTGGCATTAAAATGAGGGGGGGGAAAAGAACATCT25240.48401726660645217No Hit
CCAGATCCTTAACATTGGTCCGTATTTGAAGTCCCACTTTCTCATGGTTA25150.48229137302505043No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT24940.47826428800177956No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT24160.4633065436296309No Hit
AGAAATCCAAGGCTCTTCTCTTTTTGCAGTTCTTTTGGTCATCAATCTCT23050.4420205227923424No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTGTAAAGCGGAAGGGGCCGT21500.4122968000015341No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACAATATCACC19440.37279301358278244No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA19330.37068358809440255No Hit
GAGTCCTCCTTCTGTGAGCAAACAGTATGTATTTCCAGTCCACTTTCAGA19190.3679988647455554No Hit
AACATGTTCCAGAGCAGTTGGTAGAGTAAATGCCGAGAATCAAACAGAGT18630.3572599713501665No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT18610.35687643944318836No Hit
GTCCATGCTCTTGAAAATAATCATCTACTAACCTTTTTTATAAAACAGTG18380.35246582251293945No Hit
GTTATAAATGATATCTAAAGGTCACTAAGCTAAAGACTATATAATCTACC17650.33846690790823614No Hit
TTACTTTACCTTTCCAATTTGCTGAAGAGTGCAAACAATACATCATACTT17350.3327139293035636No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT17070.3273444826058692No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG16860.3233173975825984No Hit
TCCTTCTTTTTCCTACTGAGTTCAGCTCCCAGACTGGAATTAAGTGGAAG15180.291100717396432No Hit
ACCAGAGTTAGTAGTGTATGTAAAATTCTGACTGTTCTTGCAACAATAGC15060.288799525954563No Hit
CGCGTTGCCTTTGTTTCTTCTTAATTCGCATATCCTGTATATCTCTGGCA14250.27326648372194706No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG13010.24948750548930043No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG12900.24737808000092049No Hit
TCACTGGAAAATTCTGAAATGAAAGGGTTTTCTTTGAATTCTCTTAGAAT12710.24373452688462785No Hit
ATTACTAAGTCATAAAAATAAACCAGGTAGAATATTTACCTTCACAAACT12660.24277569711718242No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG12610.24181686734973698No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT12540.24047450567531342No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG12040.2308862080008591No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC11980.2297356122799246No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT11840.2270508889310774No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA10990.21075078288450513No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG10590.2030801447449417No Hit
TGGAAATATAAAAGCTGTGCTTGGCCTGACAAAGAGGAGGCTAGTTAGTA10470.20077895330307266No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT10450.2003954213960945No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT10020.1921494853960638No Hit
GCAGTCTGGGGCCCTTTGCTCTGCCACACACAGTCGGGAAACAAGCATAG9960.19099888967512932No Hit
TTCTTCACTTGATTGGCCTTTGCTACCGTACCCACCTAATTGTACTGAAT9540.1829447196285877No Hit
AAGTGTAGACTAATGATGTGACTTTTGAAACAGAAGCACATTAATATTTT9230.17699997507042603No Hit
CGTCAGCTATTGAATTACTTTCCAAAAGAGAAATTTCATTGAATTTTTTA8570.1643434221401464No Hit
CCCCAAAACACAGTAACCCAAATACTCACCATATTGTTAACATTCTTTAA8510.16319282641921187No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT8430.1616586987912992No Hit
CCGGACATAGTCCAGGAGGCAGCCGAAGGGCATGAGCTGCGTGATGAGTT8140.1560974861401157No Hit
CTGCACCCAGCCATAAGTAAAAGTGGCCAACTGCCCTGTAGAACCATGTC8130.15590572018662663No Hit
GGATCCGTGGCGAGTTCGTCCACTGTTACAGTGGCCAGTGGTAGGGGAGA8080.1549468904191812No Hit
AATTCTGCTGAATGGGTTGCTGGGTTGTGTCAAATATATAAGTTCATGTA7970.15283746493080125No Hit
ATGATCCATGGACATTTGACTGGTAGGTAAGTGGAGTGAAATTTAATGTA7860.1507280394424213No Hit
CCCCATGTCCAGGCTCATACGGTAGCAGAGACATGGTATATCAGCAAATC7680.14727625227961777No Hit
TGGCCATAGGTTCCTTCTCCAATAATCCCAATAATGTCAAACTTGTCCAC7680.14727625227961777No Hit
GAACGCTTTGAACTTCCCGATATCCGGTCCTTCATCGATCCCGACTTGCT6660.12771612502373103No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA6590.12637376334930744No Hit
CTGGGCGCGGGAATCCCGGCGGATCCCGGGCGGGCGGATGACCCCCAGCC6470.12407257190743841No Hit
CTGCACCCAGCCATAAGTAAAGTGGCCAACTGCCCTGTAGAACCATGTCG6330.12138784855859121No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT6300.12081255069812395No Hit
TCTGGATCAGAGTCAGTGGTTGCCTACGCGTGGGAAAACCTATCGGAAGA6250.11985372093067852No Hit
AATTACCTCCAGTTGCTCCTTCTACGTAGAGAGACTTGGGCCTCTTAGTA6100.11697723162834223No Hit
TTAGTTATCGTTTCTTTCCTTTCTTACAGCCCCCTGGCCACTGTTCTAGG6050.11601840186089682No Hit
CCACTTCTGGGTAACTTTTTAGACTCCAAACCTGAATCCTTAGCCTCTAC5850.1121830827911151No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT5770.11064895516320242No Hit
GGAAGGTGACGTGAAAGATCACTGTGCAGCAGCAATCTTGACTTCTGGAA5690.10911482753528973No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGACGTCTACAATATCAAC5570.10681363609342069No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGAATAAGCAGAAACTGCCATGCTC5560.1066218701399316No Hit
GCACAAAGGCCCCATTCTGGCACTGACCTATTGCATCCCCAGCCCCAGAC5340.10240301916317172No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)