FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69244
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA41756.029403269597366No Hit
TGAACTTATATATTTGACACAACCCAGCAACCCATTCAGCAGAATTAGAC35145.074807925596442No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC34955.047368725088094No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCCTGAAAACCAGAT33744.872624342903356No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC33544.8437409739472015No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC31664.572237305759344No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT24663.561319392293917No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC24523.5411010340246087No Hit
TTAACTACAATTCCCAGCAAGCCGCGGAGCCAACCTGGGCCTCTGCCGGC21783.1453988793252847No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG21293.074634625382705No Hit
TTAAATTTCACTCCACTTACCTACCAGTCAAATGTCCATGGATCATAGAC20352.938882791288776No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT18702.700594997400497No Hit
GGAAATTGAAGAAATCTGCCTAGTAGATGAGAACCAGTTCACCTTAACCA14482.0911559124256254No Hit
GTATAATGATGAGCATTTTGGCTACCGTACACATAGGTTAACATTTACCA12811.8499797816417307No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA11861.7127837790999942No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA11831.708451273756571No Hit
CTCATTCTTTCCTTGATTTTCTTCCTTACGGTAGCAAATACTGAGCATCA11491.6593495465311074No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTACGC9511.3734041938651724No Hit
AGACATCGCTGAATTTAAAGGATACAGATGAAATTGCTGCCACAGACCAA8351.2058806539194733No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA8111.171220611172087No Hit
CTACTCTTTAGCTTCCTACCTAAGAATAAAAATGGGATTGTTTGCACTAA7251.0470221246606204No Hit
GATTCCCGCGCCCAGAGACCAAGTCTTCTACAGTCCCACGACCCCCTCAG7071.0210270926000808No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT6440.9300444803881924No Hit
TTAGTTGCTTTTGAATTTACAGTTTAGTGAATGTGATTGATGTTTATATT5970.8621685633412282No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC5800.8376176997284964No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTACG5530.798625151637687No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT5510.7957368147420716No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC5060.7307492345907227No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC4910.7090867078736064No Hit
ATGATATAAATTTCTGGTGCTTCTTTCAGACCAAGTCTCTGCTACCGTAC4770.6888683496042979No Hit
AACAATCGACAGGCTAGAAGTTGGCAAAAGTGGTTCACAATGATCTGATG4730.6830916758130668No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTACGCG4660.6729824966784126No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTACGCGACT4510.6513199699612963No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA4370.6311016116919878No Hit
GCCTACCAGTCCAGCACCCGTGATCACACCACCTCAGCAACCAGACCAAG4180.6036624111836405No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA4070.5877765582577552No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTACGCGAC3930.5675581999884467No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAC3670.5300098203454451No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTATTGTTTGAA3510.506903125180521No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA3220.4650222401940962No Hit
CATGAGAGATATTCCAACGTGCAAGTGGAAAATCTGTAGACCAAGTCTCT3190.460689734850673No Hit
CTAACAGTACTCGGCCTGCTCGCTGGTATACCAAACTTGGATTCTTTCCT2790.40292299693836287No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT2620.3783721333256311No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACGAGA2550.3682629541909769No Hit
CTTTTTCTGGTTGGGCAGTTGGCCTGGATGATGATGTTGAAATATGTTCC2330.3364912483392063No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACAGAGACCAAGTCT2210.3191612269655133No Hit
TGATAAATGCACCTGTTTGCTGTACACTGACGACATGTTTCTTCTATAAC2190.3162728900698978No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTACGCGA2140.309052047830859No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG2130.30760787938305123No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA2130.30760787938305123No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTACGC2100.303275374039628No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACCGAGAC1840.26572699439662645No Hit
TTAAATTTCACTCCACTTACCTACCAGTCAAATGTCCATGGATCAGAGAC1820.26283865750101093No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTCTACAGTTTATTTGAGGGGAAGC1800.2599503206053954No Hit
TATTTAGTAGCCAGGACAGTAGAAGGACTGAAGAGTGAGAGGAGCTCCCA1760.25417364681416443No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT1750.2527294783663567No Hit
CAGCAGTTACCAGGGCACAGACATTCCTGCCATCAAGAGTGTCACAGACC1630.23539945699266362No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG1600.23106695164924035No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCAAGGTAATTAAA1550.22384610941020158No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTACGCGACTTGTATC1500.21662526717116287No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC1480.21373693027554735No Hit
GCTGTTCTTTTTGGCTTCATTTGTATTCTTGAAACTTACCATATATTTCT1440.20796025648431635No Hit
GGTCTCTCATGGCACTGTACTCAGTACTGAGCACAACAAGGAATGAGACC1430.2065160880365086No Hit
GGGATAATGGGGTTTTCCTGTGTACACTGACGACATTTGCTGCCATACAG1430.2065160880365086No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT1390.20073941424527758No Hit
AAGTGGTTCTGGATTAGCTGGATTGCCAGTGCGCTTTTCCCAACACCAAC1370.19785107734966206No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC1330.19207440355843106No Hit
TTTGTGCTAAAACTTTGAGTCCCATACGGTAGCAGAGTCACAGTCACAGG1320.1906302351106233No Hit
GGGAAGCTCTTCATCCTCACGAGACCAAGTCTCTGCTACCGTACGCGACT1240.17907688752816128No Hit
ATGAGCAAGTTGGGGTGTGGGGGTTGTTGACTTGTAGACCAAGTCTCTGC1170.16896770839350703No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG1110.1603026977066605No Hit
TGAACTTATATATTTGACACAACCCAGCAACCCATTCAGCAGAATGAGAC1080.15597019236323725No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC1030.1487493501241985No Hit
ACACCACTCTCTAAATCAAACACATTGGAGACCAAGTCTCTGCTACCGTA1000.14441684478077524No Hit
TGTTCATTCATGATCCCACTGCTACCGTAAGTGAAAAAAGTCTTTATAGG990.14297267633296748No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTACGCG990.14297267633296748No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTACG950.13719600254173647No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTACGCGA830.11986598116804344No Hit
GTAGACAACATAAAGCCTCATAATTACTCTGTTATTTTTCTTTTAGGCAT800.11553347582462017No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGGAGACCAAGTCT700.10109179134654267No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)