FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69244
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG54007.798509618161862No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT41005.921090636011785No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG34995.053145398879325No Hit
AATTCTGCTGAATGGGTTGCTGGGTTGTGTCAAATATATAAGTTCATGTA34725.014152850788516No Hit
GCTGTCTACCTGACCAATCGATGGGGCATTCATTATGACATGAAGATCAG33354.816301773438854No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA31674.573681474207151No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC24483.535324360233378No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT20732.9937611923054703No Hit
ATGATCCATGGACATTTGACTGGTAGGTAAGTGGAGTGAAATTTAATGTA20042.8941135694067355No Hit
ACTTTCAAGTTTCCCCACCCTTTACACAGTTTCCCCGCGGAAAACGCCGC19042.7496967246259603No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT18762.7092600080873432No Hit
CTGTGACAGTTCCCAGCGGGTCCGGATATGAATGATAGACTGGACAATGG13972.01750332158743No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT11811.7055629368609555No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT11741.6954537577263011No Hit
AGGGGTGGTAAATGTTAACCTATGTGTACGGTAGCCAAAATGCTCATCAT10091.457165963838022No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA9591.3849575414476345No Hit
AGTGAACTTGATGCTCAGTATTTGCTACCGTAAGGAAGAAAATCAAGGAA9021.3026399399225925No Hit
GTGGCAGCAATTTCATCTGTATCCTTTAAATTCAGCGATGTCTTGTAGAA8401.213101496158512No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT7981.1524464213505863No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT7061.0195829241522731No Hit
GAGGGGGTCGTGGGACTGTAGAAGACTTGGTCTCTGGGCGCGGGAATCTG7001.0109179134654267No Hit
AACATGTTCCAGAGCAGTTGGTAGAGTAAATGCCGAGAATCAAACAGAGT6440.9300444803881924No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT6330.9141586274623072No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG5750.8303968574894577No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG5550.8015134885333026No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC5500.7942926462942637No Hit
ATGAAAACACAACATGAATATAAACATCAATCACATTCACTAAACTGTAA5410.7812951302639939No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG5250.75818843509907No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG4990.7206400554560684No Hit
GAAAGAAGCACCAGAAATTTATATCATTGTAGAACCATGTCGTCAGTGTA4960.7163075501126451No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT4700.6787591704696436No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC4600.664317485991566No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT4450.6426549592744498No Hit
GGTTGCTGAGGTGGTGTGATCACGGGTGCTGGACTGGTAGGCTGTAGAAC4210.6079949165270637No Hit
ACCTCTCAGAAACTTCTCTCATTAACTAAAGTCAGCTCTCCCGCTGGGCC3970.5733348737796777No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT3960.5718907053318699No Hit
CTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG3670.5300098203454451No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA3670.5300098203454451No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC3090.44624805037259546No Hit
ACAGATTTTCCACTTGCACGTTGGAATATCTCTCATGTGTAGAACCATGT3070.44335971347697994No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT2630.37981630177343884No Hit
CGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT2610.3769279648778233No Hit
ACCTGTATAGGGTATGCTCTTTGAATAATTACATCAACACAACCAACATT2430.3509329328172838No Hit
CGGGGTGGTAAATGTTAACCTATGTGTACGGTAGCCAAAATGCTCATCAT2370.3422679221304373No Hit
CGTGAACTTGATGCTCAGTATTTGCTACCGTAAGGAAGAAAATCAAGGAA2360.3408237536826295No Hit
GGAACATATTTCAACATCATCATCCAGGCCAACTGCCCAACCAGAAAAAG2260.326382069204552No Hit
CTGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT2200.31771705851770554No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC2140.309052047830859No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA2110.30471954248743577No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA2080.3003870371440125No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT2050.2960545318005892No Hit
TACTGGTGTGGGTTATAGAAGAAACATGTCGTCAGTGTACAGCAAACAGG2010.2902778580093582No Hit
CCTTTCAAGTTTCCCCACCCTTTACACAGTTTCCCCGCGGAAAACGCCGC1930.27872451042689617No Hit
CGGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA1820.26283865750101093No Hit
CTGATCCATGGACATTTGACTGGTAGGTAAGTGGAGTGAAATTTAATGTA1800.2599503206053954No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG1760.25417364681416443No Hit
CTACCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGATCTCCCACA1750.2527294783663567No Hit
CTTAAGAAATAACTGTGATTTGGCTTCCCCTCAAATAAACTGTAGAGTTC1720.2483969730229334No Hit
GTGACACTCTTGATGGCAGGAATGTCTGTGCCCTGGTAACTGCTGTGTAG1640.23684362544047136No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT1590.2296227832014326No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT1570.2267344463058171No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG1470.2122927618277396No Hit
GTCTCTGTATGGCAGCAAATGTCGTCAGTGTACACAGGAAAACCCCATTA1420.20507191958870083No Hit
CATTCCTTGTTGTGCTCAGTACTGAGTACAGTGCCATGAGAGACCTGTAG1410.20362775114089307No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG1370.19785107734966206No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT1370.19785107734966206No Hit
AACCTACCTGTGACTGTGACTCTGCTACCGTATGGGACTCAAAGTTTTAG1200.17330021373693028No Hit
ACAAGTCAACAACCCCCACACCCCAACTTGCTCATTGTAGAACCATGTCG1200.17330021373693028No Hit
CGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC1200.17330021373693028No Hit
AGAAAGAAATATATGGTAAGTTTCAAGAATACAAATGAAGCCAAAAAGAA1150.1660793714978915No Hit
TGCACGTACCACATTTAATTACCTTGAGACTTGGTCTTCTCTTGGTATTA1130.163191034602276No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC1120.16174686615446826No Hit
CATTCTGCTGAATGGGTTGCTGGGTTGTGTCAAATATATAAGTTCATGTA1070.1545260239154295No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG1060.15308185546762174No Hit
CCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT1030.1487493501241985No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT980.14152850788515972No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG950.13719600254173647No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC850.12275431806365894No Hit
ACATAATTCTAACCTATAAAGACTTTTTTCACTTACGGTAGCAGTGGGAT790.11408930737681243No Hit
TTCTTACCTCCGTGCAACATAATAAAAAATAGGATTCCCAGCTTTGGAAG740.10686846513777368No Hit
GCCTTTTGGCTAGGTGTTAAATTGTAGAACCATGTCGTCAGTGTAGATCT710.10253595979435041No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)