FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202232
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC152907.560623442383006No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA78343.8737687408520904No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA75563.7363028600814903No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC66943.310059733375529No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC62653.09792713319356No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTACGTC55292.7339886862613234No Hit
CACTTAATGAGACCAACAGTAACACCACTGACTCTGATCCAGAAGACCAA49572.4511452193520316No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTACGT49202.4328494006883186No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT47872.367083349816053No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG47132.330491712488627No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAC46472.297855927845247No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT38271.892381027730527No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTACGTCTAT34861.7237628070730644No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG33941.678270501206535No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTACGT33711.6668974247399029No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTACGTCT32601.612009968748764No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTACGTCTA31531.559100439099648No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT30861.5259701728707622No Hit
AAGTCCTCCATTTCTGTATCCATGCGAGACCAAGTCTCTGCTACCGTACG30501.5081688357925551No Hit
GCTATTCCTACCATTCTGATGAGGTAGACCAAGTCTCTGCTACCGTACGT30291.4977847224969343No Hit
AGCAATCTGCTTGTGTGTGCGTGAGACCAAGTCTCTGCTACCGTACGTCT28671.4176787056450018No Hit
TCAGATATAAAAGAAGAGGTCTTGGCCAGTTGGAGACCAAGTCTCTGCTA27841.3766367340480241No Hit
ATGGTTGATAGAGTGGTAGACCTTGCAGACCAAGTCTCTGCTACCGTACG25491.2604335614541713No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTACG25381.254994264013608No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTACG24811.2268088136397801No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTACGTC24351.2040626607065152No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA23751.17439376557617No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG22351.105166343605364No Hit
AACAATCGACAGGCTAGAAGTTGAGACCAAGTCTCTGCTACCGTACGTCT21731.0745084853040072No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTACGTCTA20761.026543771509949No Hit
TGATAAATGCACCTGTTTGCTGTCACATTCAGAGACCAAGTCTCTGCTAC20501.0136872502867993No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTACGTCTATGATATC19960.9869852446694884No Hit
AATAGTGTAAAGCAGCATATAAAAATGACTCTAGGTAGACCAAGTCTCTG19250.9518770520985799No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTACGTCT18750.9271529728232921No Hit
ATCTCCTGGTTATTCTCAGTTTTCTTTACCAGTGCAAGACCAAGTCTCTG18320.9058902646465447No Hit
ATTTAACACCTAGCCAAAAGGCAGACCAAGTCTCTGCTACCGTACGTCTA18020.8910558170813718No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTACGTCTATG17900.8851220380553029No Hit
GAGTTTGATGCACAACTTCAAGGCAATCTCAAGACCAAGTCTCTGCTACC16630.8223228766960718No Hit
AAAGTGAGGCACTCCAGTTTTATTTAAGTATTCCCAATATAAGTAGACCA16510.8163890976700028No Hit
GGGAAAAGAACAGGCTTCACCTAAAAACGTAGACCAAGTCTCTGCTACCG16060.7941374263222438No Hit
TTTTTGTCCAGCCAGCAAATGATCAGCCTTGCAAAGACCAAGTCTCTGCT15750.7788084971715653No Hit
TGTAGTTTCTAAGGGTCGAATTCTTTAAGTTCACTGGTATTTGAGACCAA12330.6096957949285968No Hit
GACTACAGATGTGCCACCATACCCAGAGACCAAGTCTCTGCTACCGTACG11680.5775544918707227No Hit
CACAAAGGTTTTTATAAGTTCTGTGGATCTAGACCAAGTCTCTGCTACCG10430.5157442936825032No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCAGAGTATTGCCAATCATGTTC10080.4984374381898018No Hit
CTGTATGTAGTCGGTGCTGTGAGTAACAGGTAGATGTCATTTCTGAGACC9360.4628347640333874No Hit
TTCAGGAAGAAGAACGATTATCCAGAAAGACCAAGTCTCTGCTACCGTAC8730.43168242414652475No Hit
AACTGAGCGACCTCAGAGACCAAGTCTCTGCTACCGTACGTCTATGATAT8560.42327623719292695No Hit
TCCAAAGTAATATCCAATGTAAAAGATGCAGACCAAGTCTCTGCTACCGT8200.40547490011471976No Hit
CATTGGTGCTAAAAGTTTCTTGGATCACATTTTAGACCAAGTCTCTGCTA7920.3916294157205586No Hit
CACCATTTATCTGAGGACTGGAGACCAAGTCTCTGCTACCGTACGTCTAT7570.3743225602278571No Hit
AATATGTGCTTCTTACCAGTCAAATGTCCATGGATCATAGACCAAGTCTC7250.35849914949167294No Hit
TTAACTACAATTCCCAGAAGAGCCCAATTCGAGACCAAGTCTCTGCTACC7000.34613710985402907No Hit
GTAGACAACATAAAGCCTCATATGTTAATTGCTGCAGACCAAGTCTCTGC6810.33674195972941967No Hit
TTCAGGTGTATTTTGGAGGCCAGGTGTGGAGACCAAGTCTCTGCTACCGT6640.3283357727758218No Hit
AGTAATCGCATGGTGGTGGTAATTTTAAAGCCCTAGACCAAGTCTCTGCT6250.30905099094109734No Hit
CTACTCTTAATCTTCCTCTCCAGAATAATCACAAGACCAAGTCTCTGCTA4980.24625182958186637No Hit
TGCATTCTGAAAAAGCCAGAATTTACAGCTCTCCAGACCAAGTCTCTGCT4900.24229597689782034No Hit
TTCTGATGAGCCAAGTCTCTGTGAGACCAAGTCTCTGCTACCGTACGTCT4710.23290082677321094No Hit
ACACCACTCTCTAAATCAAACACATTGGAGACCAAGTCTCTGCTACCGTA4650.2299339372601764No Hit
GGGTCTGCAACAAAGCAGGTATTGACAGACCAAGTCTCTGCTACCGTACG4170.20619882115590016No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA4140.2047153763993829No Hit
TGGCTTATAAAATATTAATGTGCTTCTGTTTCAAAAGACCAAGTCTCTGC3670.18147474188061236No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC3630.17949681553858934No Hit
ATCCTGTTCCAAGTCAAGACCAAGTCTCTGCTACCGTACGTCTATGATAT3600.17801337078207208No Hit
CTACTCTTTAGCTTCCTACCTAAGAATAATCACAAGACCAAGTCTCTGCT3560.17603544444004907No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAGAGACCAAGTCTCTGCTACC3460.1710906285849915No Hit
AACTGATCACCTTGAATCTGCTTGTGTGTGCGTGAGACCAAGTCTCTGCT3370.1666402943154397No Hit
GCTATTCCTGTCATTCTGTTGAAGGTAAGATAGACCAAGTCTCTGCTACC3140.15526721784880732No Hit
GCCACTCCCACATCTCCTTACCCTAGACCAAGTCTCTGCTACCGTACGTC3050.1508168835792555No Hit
TCAATCATTCTCTACAGCCCAGACCAAGTCTCTGCTACCGTACGTCTATG2930.14488310455318643No Hit
GCTCTTCCCCCCAAAAAATAAATCAATAAAAGTTTTCAGACCAAGTCTCT2860.14142173345464615No Hit
TGCTAATTGGGCAGATAGAGCTTCGTTCAGACCAAGTCTCTGCTACCGTA2720.13449899125756556No Hit
CTTTTTAGGAGAACCCTCAATCAAAAGAAACTGAGCAAGCCTAGACCAAG2710.13400450967205982No Hit
GTAACTTGATTTGCTGTTGTAGAATGCACTGACTACAGACCAAGTCTCTG2600.1285652122314965No Hit
ATTTCTCTTCTGACCTCTCAATTTTACCTTTCGTGTTGCCAGACCAAGTC2500.12362039637643894No Hit
TGCTGGAAATGTTTTAGATTCTGGGAGACCAAGTCTCTGCTACCGTACGT2400.11867558052138139No Hit
GCTCTTCACCCTGCAAAAATAAATCAATAAAAGTTTTCAGACCAAGTCTC2340.11570869100834685No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC2270.11224731990980656No Hit
GACTACAGATGTGCCACCAGACCAAGTCTCTGCTACCGTACGTCTATGAT2270.11224731990980656No Hit
CGATGCGCGGGAAGACGAGACCAAGTCTCTGCTACCGTACGTCTATGATA2220.10977491198227779No Hit
ACTCATATCAAACCTACTAAAATTGTAGACCAAGTCTCTGCTACCGTACG2190.10829146722576052No Hit
CACTTAATGAGACCAACAGTAACACCACTGACTCTGATCCAGGAGACCAA2160.10680802246924326No Hit
GGGAAGCTCTTCATCCTCACGAGACCAAGTCTCTGCTACCGTACGTCTAT2080.1028521697851972No Hit
AGTGGAACTGTTATTATCCCATCAAATGGAGACCAAGTCTCTGCTACCGT2060.1018632066141857No Hit
AAGAAGAACAAACAACAAGACCAAGTCTCTGCTACCGTACGTCTATGATA2030.10037976185766843No Hit
TACTGAATGCAAATGATCCCAAGTGGTCCAGACCAAGTCTCTGCTACCGT2030.10037976185766843No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)