FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202232
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG152787.554689663356936No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT77673.840638474623205No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT75103.713556707148226No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG66743.300170101665414No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG62083.069741682819732No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT55792.7587127655366115No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA49282.436805253372364No Hit
TCTGGATCAGAGTCAGTGGTGTTACTGTTGGTCTCATTAAGTGTGTAGAA48722.4091142845840423No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT47502.34878753115234No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC46942.3210965623640174No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA46172.2830214802800746No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT37991.8785355433363662No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC34851.7232683254875587No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT33791.6708532774239486No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA33621.662447090470351No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC32781.6209106372878672No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT31401.5526721784880733No Hit
CGCATGGATACAGAAATGGAGGACTTTGTAGAACCATGTCGTCAGTGTAG30711.518552949088176No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG30691.5175639859171646No Hit
ACCTCATCAGAATGGTAGGAATAGCTGTAGAACCATGTCGTCAGTGTAGA30381.502235056766486No Hit
CACGCACACACAAGCAGATTGCTTGTAGAACCATGTCGTCAGTGTAGATC28721.4201511135725307No Hit
CCAACTGGCCAAGACCTCTTCTTTTATATCTGATGTAGAACCATGTCGTC27841.3766367340480241No Hit
GCAAGGTCTACCACTCTATCAACCATTGTAGAACCATGTCGTCAGTGTAG25621.2668618220657462No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG25141.24312670596147No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG24841.2282922583962974No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT24231.1981288816804463No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT23461.160053799596503No Hit
CAACTTCTAGCCTGTCGATTGTTTGTAGAACCATGTCGTCAGTGTAGATC21751.0754974484750188No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT21701.0730250405474901No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT20761.026543771509949No Hit
CTGAATGTGACAGCAAACAGGTGCATTTATCATGTAGAACCATGTCGTCA20321.0047865817476958No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT20231.000336247478144No Hit
ACCTAGAGTCATTTTTATATGCTGCTTTACACTATTTGTAGAACCATGTC19180.9484156810000396No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC18630.921219193797223No Hit
GCCTTTTGGCTAGGTGTTAAATTGTAGAACCATGTCGTCAGTGTAGATCT17970.8885834091538432No Hit
TGCACTGGTAAAGAAAACTGAGAATAACCAGGAGATTGTAGAACCATGTC17960.8880889275683373No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG17860.8831441117132797No Hit
TGAGATTGCCTTGAAGTTGTGCATCAAACTCTGTAGAACCATGTCGTCAG16420.8119387634004509No Hit
ACTTATATTGGGAATACTTAAATAAAACTGGAGTGCCTCACTTTTGTAGA16300.806004984374382No Hit
ACGTTTTTAGGTGAAGCCTGTTCTTTTCCCTGTAGAACCATGTCGTCAGT15700.7763360892440366No Hit
TTGCAAGGCTGATCATTTGCTGGCTGGACAAAAATGTAGAACCATGTCGT15470.7649630127774042No Hit
CAAATACCAGTGAACTTAAAGAATTCGACCCTTAGAAACTACATGTAGAA12320.609201313343091No Hit
CTGGGTATGGTGGCACATCTGTAGTCTGTAGAACCATGTCGTCAGTGTAG11730.5800268997982515No Hit
AGATCCACAGAACTTATAAAAACCTTTGTGTGTAGAACCATGTCGTCAGT10420.5152498120969975No Hit
TTGTGAAGAGAACATGATTGGCAATACTCTGAAGTATCTACACAATAAAA9440.46679061671743344No Hit
CAGAAATGACATCTACCTGTTACTCACAGCACCGACTACATACAGTGTAG9250.45739546659282415No Hit
TTCTGGATAATCGTTCTTCTTCCTGAATGTAGAACCATGTCGTCAGTGTA8810.43563827683057077No Hit
CTGAGGTCGCTCAGTTTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG8690.4297044978045017No Hit
GCATCTTTTACATTGGATATTACTTTGGATGTAGAACCATGTCGTCAGTG8220.40646386328573125No Hit
AAAATGTGATCCAAGAAACTTTTAGCACCAATGTGTAGAACCATGTCGTC7900.39064045254954705No Hit
CCAGTCCTCAGATAAATGGTGTGTAGAACCATGTCGTCAGTGTAGATCTC7630.3772894497408916No Hit
ATGATCCATGGACATTTGACTGGTAAGAAGCACATATTTGTAGAACCATG7200.35602674156414416No Hit
CGAATTGGGCTCTTCTGGGAATTGTAGTTAATGTAGAACCATGTCGTCAG6990.34564262826852327No Hit
GCAGCAATTAACATATGAGGCTTTATGTTGTCTACTGTAGAACCATGTCG6730.33278610704537365No Hit
CCACACCTGGCCTCCAAAATACACCTGAATGTAGAACCATGTCGTCAGTG6630.3278412911903161No Hit
AGGGCTTTAAAATTACCACCACCATGCGATTACTTGTAGAACCATGTCGT6280.31053443569761463No Hit
GGAGAGCTGTAAATTCTGGCTTTTTCAGAATGCATGTAGAACCATGTCGT4890.24180149531231454No Hit
TGTGATTATTCTGGAGAGGAAGATTAAGAGTAGTGTAGAACCATGTCGTC4880.24130701372680882No Hit
CCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT4640.2294394556746707No Hit
CACAGAGACTTGGCTCATCAGAATGTAGAACCATGTCGTCAGTGTAGATC4630.22894497408916492No Hit
GTCAATACCTGCTTTGTTGCAGACCCTGTAGAACCATGTCGTCAGTGTAG4150.20520985798488864No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT4110.20323193164286563No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG3750.1854305945646584No Hit
TGACTTGGAACAGGATTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG3650.18048577870960086No Hit
TTTGAAACAGAAGCACATTAATATTTTATAAGCCATGTAGAACCATGTCG3640.1799912971240951No Hit
TGTGATTATTCTTAGGTAGGAAGCTAAAGAGTAGTGTAGAACCATGTCGT3550.1755409628545433No Hit
CTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG3450.17059614699948575No Hit
CACGCACACACAAGCAGATTCAAGGTGATCAGTTTGTAGAACCATGTCGT3420.16911270224296848No Hit
ATCTTACCTTCAACAGAATGACAGGAATAGCTGTAGAACCATGTCGTCAG3210.1587285889473476No Hit
AGGGTAAGGAGATGTGGGAGTGGCTGTAGAACCATGTCGTCAGTGTAGAT3030.149827920408244No Hit
GGGCTGTAGAGAATGATTGATGTAGAACCATGTCGTCAGTGTAGATCTCG2920.14438862296768068No Hit
GAAAACTTTTATTGATTTATTTTTTGGGGGGAAGAGCTGTAGAACCATGT2860.14142173345464615No Hit
GAACGAAGCTCTATCTGCCCAATTAGCATGTAGAACCATGTCGTCAGTGT2720.13449899125756556No Hit
AGGCTTGCTCAGTTTCTTTTGATTGAGGGTTCTCCTAAAAAGTGTAGAAC2720.13449899125756556No Hit
GTAGTCAGTGCATTCTACAACAGCAAATCAAGTTACTGTAGAACCATGTC2550.12609280430396771No Hit
GGCAACACGAAAGGTAAAATTGAGAGGTCAGAAGAGAAATTGTAGAACCA2490.12312591479093318No Hit
GAAAACTTTTATTGATTTATTTTTGCAGGGTGAAGAGCTGTAGAACCATG2310.11422524625182959No Hit
CCCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA2310.11422524625182959No Hit
GGTGGCACATCTGTAGTCTGTAGAACCATGTCGTCAGTGTAGATCTCGGT2280.11274180149531232No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA2270.11224731990980656No Hit
CGTCTTCCCGCGCATCGTGTAGAACCATGTCGTCAGTGTAGATCTCGGTG2240.1107638751532893No Hit
ACAATTTTAGTAGGTTTGATATGAGTTGTAGAACCATGTCGTCAGTGTAG2190.10829146722576052No Hit
CCTGGATCAGAGTCAGTGGTGTTACTGTTGGTCTCATTAAGTGTGTAGAA2130.10532457771272599No Hit
CGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC2100.10384113295620873No Hit
CCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG2060.1018632066141857No Hit
TGTTGTTTGTTCTTCTTTGTAGAACCATGTCGTCAGTGTAGATCTCGGTG2060.1018632066141857No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)