FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences627552
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATCTCATCGTCTTATATTGGCTTCACTGTCTTTCAACAGGCCTTGCT104721.668706338279537No Hit
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA101661.6199454387843557No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA98101.5632170720513996No Hit
CCAGTGTTGTGTTTCCCAAAGTCTTTCCTGTTGTGTAAGTATCTCCTTTT94481.5055326092499108No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC91911.4645798276477486No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG82471.314154046198562No Hit
TGAACTTATATATTTGACACAACCCAGCAACCCATTCAGCAGAATTAGAC76561.219978583448065No Hit
GCCAAACGAAAATTATGGCAGGTTGTTACGAGGCATTGGATGATTCAGAG69091.1009446229157105No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA68821.096642190607312No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC68601.0931365050226913No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC68471.09106496354087No Hit
TATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTGA68451.090746264851359No Hit
TTAACTACAATTCCCAGCAAGCCGCGGAGCCAACCTGGGCCTCTGCCGGC66891.0658877670695017No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC66531.060151190658304No Hit
CAAAGAGACTCACCTAATTTTCTTACATTTTGTTTTTGTAGGTAATTTTA66431.058557697210749No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT66121.0536178675233288No Hit
CTCAGTCACACATGCCAGAGATTGCTCAGGAAGCAATGGAGGTAAGGGGA63121.0058130640966805No Hit
GGAAATTGAAGAAATCTGCCTAGTAGATGAGAACCAGTTCACCTTAACCA63041.0045382693386364No Hit
ACGCCTTCTGTCTGAGCACCCAGAATTAGAACATATCATCTGGACCCTTT62750.999917138340727No Hit
TGTTTCAGGAAGGAATGTTCCCAATAGTAGACATAAAAGTCTTCGCACAG62450.9951366579980624No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCGATATATGT62220.9914716230686859No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT61850.9855756973127328No Hit
ATTTGTTGCAGGTTTTGGTTTTAATGGCTTGTGGCGGTTTGCAGGACCGT61150.9744212431798481No Hit
TCAATGCAGAGGTTGAAGATGGTATGTTGCCAACACGAGCTGACTCTGGG59670.9508375401560348No Hit
GGCCAGGGGTTGTGCTTTTTAAATTTCAATTTTATTTTTGCTAAGTATTT59120.9420733261944827No Hit
TATGTCATGATTCATCTTACTAGCCTCAAACATATCTTCTTTGCCAGGAC58420.9309188720615982No Hit
TGTACTTGAAAAAGGACTTTAGAGCAAGCTTAAACTAGATTGCTTTTAAA57640.9184896231706695No Hit
CCTCAGGGCCTCAGAGAAAGGCAAAAGGGCCTAGACTTTGACTTCCTTTT57070.9094067105196063No Hit
CGATGCGCGGGAAGACGTTCCGCTTTGAAATGCAGCGGGATTTGGTGAGT56440.8993677018000101No Hit
GGGATCGATGAAGGACCGGATATCGGGAAGTTCAAAGCGTTCGAATGAGG55720.8878945489776146No Hit
TGTGATTATTTCTATTTTAGCAACCAAAGGACACAATGAGATTAGATGAA54580.8697287236754883No Hit
ACACTTGGCCCTGTCACTTTTTAGAAAAGAAAGAAAAGGAAGTGCCAGGC54190.863514099230024No Hit
GTAACTTGATTTGCTGTTGTATTTGCTTAGGAAGAGCAGCACTTCAGAAA54160.8630360511957575No Hit
GTGTTTGTTCCAATACAGCAGATGAAATATTACCTAGATCTTGCCTTGGC53470.8520409464076284No Hit
GCTATTCCTACCATTCTGATGAGGTATATAATGATTCAGGATATCTCTCA51660.8231987150068839No Hit
ACTGCAGTTTTCTGTCGTGAACGTTTCTTATTTTTTGGTAGAACCAACAA50690.8077418285656008No Hit
GCAAACAGAAGGTTCCTTGAGCACTGTGAGACAACACACTATGGCATGAA49340.7862296670236092No Hit
ATTTACCCTACCAATTGCCCAGTGCGGGACTACCAGCTGCACATTTCCCG48720.7763500076487686No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAAAATGTTAGTACTGTTTGAA48710.7761906583040131No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG48550.7736410687879252No Hit
TTTCATATAGGCTGTGCAACAAGTCTCTGCTACCGTAGGGACAGTGATGA48180.7677451430319718No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT47890.7631240120340625No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG47810.7618492172760185No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT47770.7612118198969966No Hit
AGCAGGATTTTTGCTACTGATTTCTTCCTGTTCCTTTAGTCTTTTCCCAG46160.735556575391362No Hit
TTCTGATGAGCCAAGTCTCTGTGACTGTGATGTACATAAACATAATCAAA45420.7237647238794555No Hit
GGATACAGTAGCCAGAATGACGACTCAATCAGTGCTTCGGTTGTTGCTTT45020.7173907500892357No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA44720.7126102697465708No Hit
CGCCTCATGTGGTTTTATGCAGCAGATGCAAGGTATTCTGTAAAGGTTCT44290.7057582479220845No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA44250.7051208505430626No Hit
TTAAATTTCACTCCACTTACCTACCAAGGGCCTGGTTTAAGGCCCTGTAT44070.7022525623374637No Hit
CCAGCTCACAAGAGAAGAAAATACTGCTATACGTACTCCAGAACATTTAA42440.6762786191423181No Hit
TGCTCAGCCAATGGTAAAGAGACTTCAGTTAAACTTCCAAAACATTCAGA42060.6702233440416092No Hit
GACAGCAGAACACACATACCAAAGTCAGTACTGAGCACAACAAGGAATGT41230.6569973484269033No Hit
CACGTTTTTCCCATCTAGCAATTCCATGCACTTCTCATTTCTGTTGAAGT41080.6546071082555709No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA40530.6458428942940186No Hit
GCTGTTCTTTTTGGCTTCATTTGTATTACTGAGTATTTTTCTCATAGAAA39400.6278364183366478No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC38920.620187649788384No Hit
GATACCTAACTTGTCATTATCTGGAGTTCAAAAACACTACCTTAGATCAT38800.6182754576513181No Hit
GGATCCTGGGTGTTTGTATTTGCAGTCAAGTCTTCCAATTCACTGCACTG37750.6015437764519912No Hit
CTCTCTAGGGGGTCTGTCTTCTGGGCATTGATGGCAAATCATTAATGTAT37300.5943730559379939No Hit
TTAAATTTCACTCCACTTACCTACCAGTCAAATGTCCATGGATCATAGAC36660.5841746978736424No Hit
AAGTCCTCCATTTCTGTATCCATGCGTTTAGGACTCAGTTCCTCTGGAAA35990.5734982917750242No Hit
AGACATCGCTGAATTTAAAGGATACAGATGAAATTGCTGCCACAGACCAA35710.5690365101218704No Hit
GCAACAGCTTCATTGCTGAGGGACTAGACATCACTCGCCTGTAACTTGAA35260.5618657896078731No Hit
CGGCCCCTTCCGCTTTACGTCTGACGTCACGCCGCACGCCCCAGCGAGGG35130.5597942481260517No Hit
CCAGGGTAGGTCAAAAGTATCCTTTGATTGGAAAAATCTAATGTAATGGG35040.5583601040232523No Hit
GCCCTCTCAGAAACATCTGCAGGAGGACTTGGGGAAACAAATTCATATGA33820.538919483963082No Hit
CGAAGGGAGAACGACGAACGTCGTGGATCAGATCGGAGCGACCGCCTGCA33200.5290398245882413No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA32610.5196382132476671No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC31760.5060935189434501No Hit
ATTTAACACCTAGCCAAAAGGCAGAAATTACAGAACTTTCTACTATATTA31690.5049780735301617No Hit
CCTCTCTCCTTCTTCCCCAAGTACACACACAAACCTGCCAATGCCAGTGG31070.49509841415532096No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA30540.4866528988832798No Hit
CCACAGAGATAGCACCTAGAAAGCTGAATTAAGCAAGGAGGGGCAGAACA30440.48505940543572484No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG30200.48123502116159295No Hit
GGGATAATGGGGTTTTCCTGTGTCACTGACGACATTTGCTGCCATACAGA30000.47804803426648307No Hit
CCTCAGGGCCTCAGAGAAAGGGTGGGGAAACTTGAAAGTAGACCAAGTCT29600.47167406047626326No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTATGT29260.46625618275457653No Hit
TTCTGATGAGCCAAGTCTCTGTGGGAGGAGGGCAGTACACAGACCAAGTC28120.4480903574524501No Hit
CTTTCTAGTTGATACGGCCTTCACTATGTAAAGGTCAGTCTTTTTATTTC28050.44697491203916173No Hit
ATCTGGTGTTGAAAATTCTAATGACTTTGCATTTTTGAAGGTTTTTCCTT27730.4418757330069859No Hit
AGATAACTCTGTCATTTTCCTACTTGTTCAGTCCATGGTGGTTGATCTTA26100.4159017898118403No Hit
AGCAAGCTGTGACGGGTAAGCTTTATAAGGAAAGTAAATCTTCACCCCAC24530.39088394268522764No Hit
GGGGACGCTCTTGTATTATCTGTACACTGACTTATGGTATCAAATAATAA23850.3800481872418541No Hit
TCCCCTTTGCTTTGTCTTTTTCCAGCCTCCCCCACTGGCAGGATTGCCAT23310.37144332262505736No Hit
GCTTCCAGTACTTCAACTTTGCCCCCTTCTACTCACTCAAAGACATCTGC23190.36953113048799147No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC22780.3629978073530162No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC22700.3617230125949722No Hit
TCAAATGTAGCAAATCAGAAGCCCTTTGAGAGTGGAAGTGACAAAATCTC22180.3534368466676865No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA21880.34865636632502167No Hit
CTGTATGTAGTCGGTGCTGTGACTTGTTTGTGCTCATCTCTGTTCTGTAG21400.34100759777675793No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCTGATATATGT21220.33813930957115906No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTATGTT21120.3365458161236041No Hit
GTCTCAGAACAAACCTGAGATGCATGACTACTTCCCATAGGCTGTTCTAA20430.325550711335475No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC20320.32379786854316456No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTATG20160.32124827902707664No Hit
TTTCTTACCCTGTGCAGAGCATTTACGAAGTGATAAATGCACCTGTTTGC19710.3140775585130794No Hit
AGGTTCAACTACGCGTGTAAATTCTACTGCAAATGCAGAGACACAAGCAA19530.31120927030748047No Hit
GTTCTTCCTCAGACATTCAAACGTGTTTTGATCAAAGAAGAGGAGTATGA19410.3092970781704145No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAAA19240.3065881393095712No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTATGTTACG19060.30371985110397226No Hit
AATCTCCAAGGAAGTTGTACCGTACACTGACGACATGGTTCTTTGCCACG18460.2941588904186426No Hit
CGAACCCTCTCTGGGTGATAATTACACAAATCTTCATAGATGATATACTG17720.28236703890673603No Hit
ATTTCTCTTCTGACCTCTCAATTTTACCTTTTCTAGATGTATTCTTGGGG17390.27710851052980473No Hit
ACATGTACATAGGGTTTATATATCATCAGCTATATGACTTATTTAATTTC17290.2755150170822498No Hit
GCTTAGATTCCTCTTTGGAGTCAGTGTGATTTCCCCTTTTTGAGTCCCCA17180.2737621742899393No Hit
GAAGTTCATCCTGTTTTAAGTCACACTTGTGATTTGTTAAATTTTTTAAC16720.2664321044311866No Hit
TGGATCCTTTATTACGAATTGTGATCACATCCTCTGATTGGCAACATGTT16500.2629264188465657No Hit
CTGTGGTGCCACCTAAGCTCTGCTACCGTACAGCAAAAAGTCCTGCAACT15320.24412319616541736No Hit
AGTGGATGGTATTGCTTTTCCATTTCGTCATGACCTAGATGACCTGTCTC14550.2318532966192443No Hit
TGAAAAAGAGCAAGGTACTAGTGAAATCACCAGTTTTAGCCATCAATGGG14520.2313752485849778No Hit
AGTTTTTGTGTTGATGAAGAGGAGTCTTGCAAAGGCCAATCAAGTGAAGA14300.22786956300035696No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC13840.22053949314160423No Hit
AATAATGAGTTTGGTCATCTGAACTTTTAATTAATTAAGTTCATGTGTTT13030.2076321962164092No Hit
GGGCAGTTGGCCACTTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTG12970.20667610014787618No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAT12760.20332976390801083No Hit
CTTAAATGTTGAAGGTGGTTCTTCAGAAAATAATCACTCTATTAAAGTTT12210.1945655499464586No Hit
CCCATTTTCAAGAACTCTACCATGGTTTTATATGGAGACACAGGTGATAA12130.19329075518841465No Hit
TGAAGAAGGAGATGGTGTGGAATTGATGGAAGCCAAATCACAGTTATTTC12100.1928127071541482No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT11880.1893070215695273No Hit
AAAATTTGTTTATAGTCATCCAAAGTTAAAGAAATTAGAAGAAGTTGTAA11620.18516393860588445No Hit
GTATAATGATGAGCATTTTGGCTACCGTACACATAGGTTAACATTTACCA11410.18181760236601907No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTATGTTAC11370.1811802049869971No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTATGTTA11320.1803834582632196No Hit
TCCAAAGTAATATCCAATGTAAAAGATGCAAATGCATACCCACAAACTGT11110.17703712202335423No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAATTCTTTAACTTACTAAAA11090.17671842333384324No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG11000.1752842792310438No Hit
TTCTCGGGCTGTGTAATATGGACAGGCCTTTAGTTTCTTCCCCAGGCTGA10990.1751249298862883No Hit
TGGTGATAGATGACTTGCTGCTACCGTAAAGCCTGCAGTGATATTAACTG10950.17448753250726634No Hit
GCAGAACATTTTGTTTCCTCACTAAGGTGATGTTCCTGAGATGCCTTTGC10940.17432818316251084No Hit
AGCTGCTCACCATCGCTACCGTAACTGCTATTCTTGACAAGCTGATAACA10860.17305338840446688No Hit
GTTTTTGTACAGAGAATAGTTGTAGTTGTTGAATTCAGTATCATCCTATG10590.16875095609606852No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTGACCACATTTAGCTGGGACACAT10490.16715746264851358No Hit
TCAGTCTCATCTGCAAATACTTGTGGGATTTTTAGCACAGCAAGTGGAAA10010.15950869410024987No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC9780.15584365917087348No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC9190.14644204783029932No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT9140.14564530110652185No Hit
TTGCACAAGGTTTGAATCAAGTAAATTTGAAATAGATTGAAGTTAAATAC9010.14357375962470043No Hit
GCACGGTTTCTGTAGCCCATACTTTGGATGATAGAAACTTCATCTTTTAG8580.13672173780021418No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTATGTTACGATCATC8250.13146320942328285No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTATGTT8200.13066646269950538No Hit
CATCTCCATGAGTAGGATGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC8150.1298697159757279No Hit
TCTATTGTGATGGCCACGTTTTCCATTATTTTCTAATGGCAAAGATTTCT8110.12923231859670592No Hit
TGAATGTAGCACGCATTCACATAAGGTTTTTGCTGACATTCAGAGTGAAG7860.12524858497781857No Hit
TCTTGGTTGTCAGTGCTTCAGTAAAGCTTATTTATTTATTTTTTTCTAGC7750.12349574218550813No Hit
CATCTCCATGAGTAGGAAGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC7520.11983070725613176No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA7340.11696241905053287No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG7330.11680306970577738No Hit
GGGCAGTTGGCCACTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTGC7210.11489087756871143No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG7120.11345673346591198No Hit
ATCCTGAGTCTTATGTCTGATACCATGTTTTTGTTTTGTTTTTAGAGTCT7030.11202258936311253No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG7010.11170389067360156No Hit
ATGCAATTCAAATAAAAACGAAACTGTGTCGTCAGTGTGGAGACAAAAAC6920.11026974657080212No Hit
CCAGTGGCGACCAGAATCCAAATCAGGCCTTCTTACTTTATTTGCTGGAG6870.10947299984702463No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT6660.10612666360715925No Hit
CTTTCCCAAAACATGGCACTCACATCTACGGAACAGGAACCTGAAGGATT6590.10501121819387077No Hit
GGTAGGATGTGATATTCCTTCTAGTGGTACGGTAGCAGAAAGTCAGTCCC6580.10485186884911529No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTATGTTAC6480.10325837540156035No Hit
CAGTAGAGGCTAAGGATTCAGGTACACTGACGACATGGTGGTTGATCTTA6440.10262097802253838No Hit
GTCTATTTCTTCCTCCTTCTAATCTCTCTCGATTTATTTATTTTTTTAAT6370.10150553260924991No Hit
TGTCTTTTCTTCCCTAGTATGTACACTGACGACATGGTTCAGCTTTCGTT6330.10086813523022793No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)