FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences627552
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT144652.304988271888226No Hit
CAGCTGTGCCATTTGAGGGATGCTCAGGTCGGTTTGGCTCTGCAGCAGGT105591.682569731273265No Hit
GTAGTCAGTGCATTCTACAACAGCGCGCATGTTAGCAAGTTCATCAACCA99251.5815422466982816No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG94461.5052139105603999No Hit
ATCACAATATCTCATTTTGCCCTCTTTGCAACCAGTGCACATTACTGGGT90711.4454579062770894No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG89931.4330286573861608No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT81271.2950321248279026No Hit
AATTCTGCTGAATGGGTTGCTGGGTTGTGTCAAATATATAAGTTCATGTA75301.1999005660088724No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA67831.0808666054765184No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT67731.0792731120289634No Hit
GTTGTAAAATTTCTTCACTCTGAATGTCAGCAAAAACCTTATGTGAATGC67551.0764048238233646No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA65961.051068278007241No Hit
GAAAACTTTTATTGATTTATTTTTTGGGGGGAAATTTTTTAGGATCTGAT62210.9913122737239304No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT61750.9839822038651778No Hit
ACTAAAATGTTTATCTGATTAATTACCCACCTAATGCAGGAACAAGCAAG61570.9811139156595788No Hit
CAGAAATGACATCTACCTGTTACTCACAGAAACCACACACCAAAGGAACA61330.9772895313854468No Hit
CTGTGACAGTTCCCAGCGGGTCCGGATATGAATGATAGACTGGACAATGG61240.9758553872826475No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC60970.9715529549742492No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTGCTTGATTTTCTTACTTGGTCCAA59190.9431887716077711No Hit
ACTTTCAAGTTTCCCCACCCTTTACACAGTTTCCCCGCGGAAAACGCCGC58890.9384082912651063No Hit
ATTGTGTGTTCTTAAAGCAGGCATACTAATTTGAACAGAAACCTTCATTT58090.9256603436846667No Hit
CGCGTTGCCTTTGTTTCTTCTTAATTCGCATATCCTGTATATCTCTGGCA58050.9250229463056447No Hit
AGCTTCATCATACCTTTCACTAAGACAAGAATTTAGAAGTGGACAGGAAA57030.9087693131405844No Hit
CCTCAGCTTGCCATCAGGAGTCGTTACCTTTGCTAAGCTCGGAGGGTTTC56020.8926750293202794No Hit
CTGGGTATGGTGGCACATCTGTAGTCCTAGCTGCTCAGGAGGCTGAGGTG55440.8834327673244607No Hit
TCTAAAGTATGCAGGTTTTGTTCAAGAAGTGCGGTACCTGCTGAATACAA54840.873871806639131No Hit
ACTTTGTAATTCAACATTCATCGTTGTGTAAATTAAACTTCTCCCATTCC54660.8710035184335323No Hit
CAACTTCTAGCCTGTCGATTGTTAACAGGTCCAAGGAAGAAGTCACCTCA53560.8534750905104279No Hit
GGTCTTTGTGCCCAGACTTCAGCTCCCGTTCTTTCCTGGTCTTCTCCTTG53550.8533157411656724No Hit
GCAGAATTCCGAAGTTCAGCTGCATGCTGGTTTCCTTCACGACAGGTGTG51730.8243141604201724No Hit
CCTACCACAATATTCGGAGATGGGTTTTTCCTAAACTGATCTCTTGCCAG51460.820011728111774No Hit
AAGAGGCTTCCAAATTGAGGGTGGGCGCCTGGGAGGGGTGTTTCTGTTGC51180.8155499464586201No Hit
CATAGGTTTTAAAAGTTTTGCCACTTAATTAGCACTTTCTTTGCATCTAG50380.8028019988781806No Hit
ACATGCACAGTTGCTCTGGGAGTCTTCAGAATAGAAACTACCCATCTCAA49370.7867077150578756No Hit
GGATTCATTCATGCCATAGTGTGTTGTCTCACAGTGCTCAAGGAACCTTC49180.7836800775075213No Hit
TCATTTACAGTTTGTGGGTATGCATTTGCATCTTTTACATTGGATATTAC48850.7784215491305899No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT47540.7575467849676203No Hit
CTGTGTCACCAAGGGTTTCGTTGGGGCCATGAAGACCACCTTTCCTGAAG47480.7565906888990872No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT47300.7537224006934884No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC46860.7467110295242466No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG46570.7420898985263372No Hit
TCTCTTTAATTTCATCACTGTCCCTACGGTAGCAGAGACTTGTTGCACAG45650.7274297588088318No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC42540.677872112589873No Hit
CCCCAAAACACAGTAACCCAAATACTCACCATATTGTTAACATTCTTTAA42480.67691601652134No Hit
GGTTGAACTAAAACGACAATACAATGACCACCATTCAAAATTGAGAGGTC42370.6751631737290297No Hit
ACCCAAGAACATTTTCCCCACAGGGTCAGTGACCCTAGTGGTGAGCAAAA42260.6734103309367192No Hit
CACGCACACACAAGCAGATTGCTCAGATTACTAGAGAGCTCCGGAGGACG41400.6597062872877466No Hit
GAAGATGGTGAATTTGACTCCAAAGAGGAATCTAAGCAAAAGCTGGTATC41350.6589095405639691No Hit
ACTAGTATTCTGAGCTGTGTGCTAGAGGTAACTCATGATAATGGAATATT41100.6549258069450818No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA40960.6526949161185049No Hit
AGCAAACTTTCATCTTGATCAGTGGACAATACTGGGAGGTCACTGTTATC40930.6522168680842384No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT40220.6409030646065983No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG39020.621781143235939No Hit
ACTTGCTTTCCACTTGCTGTACTAAATCCAGAGAAAGCAGATGAATTTAC38950.6206656978226506No Hit
GCAAGGTCTACCACTCTATCAACCATAAAACTTCCCTCTGTATCAATAAA37980.6052088113813676No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGAAGAATAGCTTAAATG37890.6037746672785681No Hit
AGGAAATAATGAGCGAACCCCACTTCCCCACAACGGCACCCCACATGAAA37630.5996315843149252No Hit
CTTGCCCTAGATGTTCAGAGCTGCCTTCAGTATGATTATCTAGCAAGTTC37040.5902299729743511No Hit
ATGATCCATGGACATTTGACTGGTAGGTAAGTGGAGTGAAATTTAATGTA36160.5762072306358677No Hit
GTGGCAGCAATTTCATCTGTATCCTTTAAATTCAGCGATGTCTTGTAGAA35690.5687178114323594No Hit
GGAAACTTGTAAGGGCTTCGAGGAATGTGAGGTATTGGTGACAAGGTAGG34750.5537389730253429No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACATAGTGAAATGTAG33880.539875580031615No Hit
ACCTGATGAAGACTTTGGACCTCTTAAGCTTGAAAAAGTGAAGTCCTGCT33560.5347764009994391No Hit
GAACGCTTTGAACTTCCCGATATCCGGTCCTTCATCGATCCCGACTTGCT32890.5240999949008209No Hit
TCCTCAGCAGTCTGGAACCCCGCAGACACCAGCTTCACCCGCACCGCTGG32830.523143898832288No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT32170.5126268420784253No Hit
TGCCTGAATGTTCTTCACCACCTTCATGCAAACGTAAAGTTGGTGGTACA31640.5041813268063842No Hit
TGTGGTTTAGCCCTCGAAGTAAGAAAGTCAGATATGTTGTGAGTAAAGCT31320.49908214777420834No Hit
CATCTGGTTTTCAGGCACTTCAAATGTACTCTTCTGCAATATGTAGCTTG31300.4987634490846974No Hit
CCCCCTACTCCCTCTTATCCAAAGTCCTTGCTTTTCTTTTCCACCGCTTC31270.49828540105043084No Hit
GCAGTCTGGGGCCCTTTGGCAGGCCCTGGTTGCCCTGAGGCGGGTGCTGC31120.4958951608790984No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC30150.4804382744378155No Hit
GTCTCTGTATGGCAGCAAATGTCGTCAGTGACACAGGAAAACCCCATTAT29570.4711960124419968No Hit
ACTTTCAAGTTTCCCCACCCTTTCTCTGAGGCCCTGAGGTGTAGAACCAT29400.4684870735811534No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA29160.4646626893070216No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG28580.45542042731120286No Hit
GTGTACTGCCCTCCTCCCACAGAGACTTGGCTCATCAGAATGTAGAACCA27570.43932614349089794No Hit
GTAGACGATGTAAAGCAAGCACATTGCCGTCACTTATGAAGGAAAGACTA27490.438051348732854No Hit
TGCAAGGACCGTACAGTGCCTCAGTGATGCCAGCCTCCGAGACATGCCAC25550.4071375758502881No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACAATATCACC24680.3932741828565601No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTACAGATAATACAAG23520.37478965886492277No Hit
TTCTTTGTAAGGCGTGTCTCAATATTTGACATTTTCATTTCTGTTGTAGT22920.3652286981795931No Hit
ACAACAAAAGCAAGTCCTATGAACTTATCAACGAAGAGTCAGAACTTTAA22890.3647506501453266No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG22630.36060756718168374No Hit
CAGTACTTGTCCCTGAGGTAGTTTCTTTTCGAGAAGTTTTGGATGGAGGT22630.36060756718168374No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG22540.3591734230788843No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT21880.34865636632502167No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACGATATCACC21820.3477002702564887No Hit
TCTCCAGGTAATAAGGGTGGGAGGGGCAAAGGAGGCAACGTTGAAGTTTT21570.3437165366376014No Hit
GGCTAGAAATACGTGGCAAAGAACCATGTCGTCAGTGTACGGTACAACTT21180.33750191219213704No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT21100.3362271174340931No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTATTTTCTCCATCTGGGCTC20530.32714420478302997No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG20120.3206108816480546No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG19990.3185393401662332No Hit
CACAGAGACTTGGCTCATCAGAAATATTATCATTAGGTATATCATAAAGG19170.3054726938962827No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC18980.3024450563459283No Hit
AGATTAGGGGTTTTGCAACCTGAGGTCTATAAACAAAGTCTTCCTGGAAG18910.3013296109326399No Hit
AGAAACAGCTAATAAAAAGTTCTCCAAACTTACTGATTTTTTGTTTTCCT18750.2987800214165519No Hit
GTGGACCCATTACATTAGATTTTTCCAATCAAAGGATACTTTTGACCTAC18540.29543368517668656No Hit
GTCCATGCTCTTGAAAATAATCATCTACTAACCTTTTTTATAAAACAGTG18510.29495563714242007No Hit
GGCATTCCTTTTTGAACAGTACCCGTTCCCTTGATGTCTACAATTTCACC17800.28364183366477996No Hit
CACTCAAGAGCACATGTACAGCTGTACCTGATCAGTTCTAGCTGAGCCAG17670.28157029218295854No Hit
CACCATCCAAAGGACAACCTTATTAAGCTCGTCACTGCAGGTGGGGGCCA17570.2799767987354036No Hit
GGGGACTCAAAAAGGGGAAATCACACTGACTCCAAAGAGGAATCTAAGCT17030.2713719341186069No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTAGGCTGCAGCGACTTCGAACT16850.268503645913008No Hit
TACCTTTGAAAACGGTCCTGCAAACCGCCACAAGCCATTAAAACCAAAAC16160.25750854112487886No Hit
CAGGCAAACGCTATTTTGACATTTCTAGACATTAACATTTTATACAGTCT14430.2299411044821784No Hit
TAATGATCAAGGGGAGAAGGTGCCTTGCTTCATGCAGTGTTAGTAAAACA14220.226594768242313No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA13870.2210175411758707No Hit
ACAAGTTGCAGGACTTTTTGCTGTACGGTAGCAGAGCTTAGGTGGCACCA13760.21926469838356022No Hit
TAGGTGGCACCACAGTCTCAATAGAAACAAGGTTTTTATCATTATTGAGA13400.21352812197236246No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGACGTCTACAATATCAAC12860.20492325735556577No Hit
CTGCACCCAGCCATAAGTAAAAGTGGCCAACTGCCCTGTAGAACCATGTC12830.20444520932129928No Hit
CCGCCCATCAATATCAAAATCCCACTCTAATCAAATAATACTGACTTTGA12810.2041265106317883No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA12710.20253301718423333No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT11830.18851027484574984No Hit
CTGCACCCAGCCATAAGTAAAGTTTTACCTCTTGCAATAACTTTGTCTTG11500.1832517464688185No Hit
ATATTCAAGGAGATGTCCGATTTTAAATCTTGACCTAGAGTCATTTTTAT11440.18229565040028553No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC11230.17894931416042018No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT11230.17894931416042018No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC10930.17416883381775533No Hit
CACATTCCATAGCTGCCAGTTTCCATATGATCCATCTATAGTGATTATAA10810.1722566416806894No Hit
GTTATCAGCTTGTCAAGAATAGCAGTTACGGTAGCGATGGTGAGCAGCTT10780.17177859364642292No Hit
CCCTGCTTCCAACACTTGTTATTTGGTAAAGTAAACAATATACCTTCTCA10760.17145989495691194No Hit
CAGGTGGCCCTACCTCAAAATTATTACTATTAGATATGGACAATTTAATG10700.17050379888837897No Hit
ACGTTTTTAGGTGAAGCCTGTTCTTTTCCCAAAACATGAATGTTCTCAAC10670.1700257508541125No Hit
AGGGGTGGTAAATGTTAACCTATGTGTACGGTAGCCAAAATGCTCATCAT10660.169866401509357No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACAATTTTGTTCCATCATTTCT10600.16891030544082403No Hit
CCTGTCATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTA10330.1646078731324257No Hit
ATGTGTCCCAGCTAAATGTGGTCAGTTCTTTCACTTCCCCTCAAATAAAC10290.1639704757534037No Hit
AGACAGTTAATATCACTGCAGGCTTTACGGTAGCAGCAAGTCATCTATCA10170.16205828361633776No Hit
TGCTAATATTGGTTCACATTTCAGTTATGCATATTTGTTTCTAGGGAAAA10160.16189893427158228No Hit
TTACTTTACCTTTCCAATTTGCTGAAGAGTGCAAACAATACATCATACTT10130.1614208862373158No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG9800.1561623578603845No Hit
TGTGAGCTGGTCTGAATGTTCGTTACTTTTAAACAATACTTTGGAAAAGA9650.15377211768905205No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG9020.1437331089694559No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT8770.13974937535056856No Hit
TGTTCATCATTGGACTACGCAAAGATTTCATGTAAATAGCTCTCATTTCA8520.1357656417316812No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT8360.1332160522155933No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT8070.12859492121768396No Hit
GAGACCTACATCAGGCCTTCATCCTACTCATGGAGATGTGTAGAACCATG8000.1274794758043955No Hit
GCAGATGAGACTGACTTATGAAGCTTCCCTATACTACATTTACATATATC7770.12381444087501912No Hit
ACACAACAAAGAGCATACATAGGGTTTCTCTTGGTTTCTTTGATTATAAT7770.12381444087501912No Hit
GGAAGTTTAACTGAAGTCTCTTTACCATTGGCTGAGCAAATAGAGTCTCC7720.12301769415124165No Hit
GAGACCTACATCAGGCCTTCTTCCTACTCATGGAGATGTGTAGAACCATG7420.11823721380857682No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT7370.11744046708479934No Hit
ATGATCCATGGACATTTGACTGGTATTTCCACTAGAAGGAATATCACATC7290.1161656723267554No Hit
CTGCACCCAGCCATAAGTAAAGTGGCCAACTGCCCTGTAGAACCATGTCG7240.11536892560297793No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG6900.10995104788129112No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT6820.10867625312324716No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT6760.10772015705471419No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT6600.10517056753862628No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT6580.10485186884911529No Hit
TTCTGGGGCTGTTTTTGTCTCCACACTGACGACACAGTTTCGTTTTTATT6490.10341772474631586No Hit
CCATGGGACTGACTTTCTGCTACCGTACCACTAGAAGGAATATCACATCC6470.10309902605680485No Hit
AAGACTTAAATTTTACATACCTACTGTTTCATTGAATTATAATTCATCAC6310.10054943654071694No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)