FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407132
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT200754.930833243272452No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA184754.537840307320476No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA177284.354361730347897No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC168554.1399349596691No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC123183.0255543656602772No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT115712.842075788687698No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA103242.5357869192301266No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT98262.4134678679150743No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA95492.3454309658783883No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC90072.2123046088246565No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG89052.187251309157718No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC85672.104231551437863No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG74181.82201349930735No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG72621.7836966880520322No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG66431.6316575459556115No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA65051.5977619052297536No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT57731.4179676370317245No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG54671.342807738030909No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG53011.3020347209258913No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT51451.2637179096705737No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC51181.257086153876384No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG46581.144100684790191No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT44351.0893272943418841No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG43441.0669758211096156No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC41411.0171148423607086No Hit
CAGAGGGGAAAAATATGACAAAGTCTGTGCTAAAATGGAGATTCTCTGTT40951.0058162954520893No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT39470.9694644488765315No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA34770.8540227739406384No Hit
TCCCTGCCAGCGAGATCTCCTCCATCTTGGAGAAAGGAGAACGCCTCCCT33230.8161972038552607No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC31830.7818103219594628No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA31290.7685468103710836No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG29590.7267913109261861No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT28570.7017380112592476No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA28360.6965799789748779No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC27620.678404055687099No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGAGTGGGAGGCTG24540.6027529155163436No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC23260.5713134806401855No Hit
AGGACAAAATGTTTCACTTTTGGGTAATACGGTGCAGATAATGACAAGGA23090.5671379306956957No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA22310.5479795250680369No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA22090.5425758721986972No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG20010.4914867905249403No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG19820.4868199994105106No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTAATGTGGGGGTT19080.46864407612273173No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC18740.46029297623375215No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG17090.41976557971370465No Hit
GTAGAGGAGCCGTCAAATCCAAGTCTCTGCTACCGTATCAAGAGGGATAA16820.4131338239195151No Hit
GACCCAAAACCCAAAATGGCAGGAAAGGGAGAGATGGGGGTGGGAGGCTG15830.3888173860074865No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGTCGTGTACTAT15660.38464183606299673No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT13430.3298684456146901No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC13170.32348231040547043No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTGCTAATGAGTTTGTACTGAG13170.32348231040547043No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGTCACAGGCTCCCAG13160.32323668982050047No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTAGGTCTTCAGTGAAC11530.2832005344703929No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGGCTAATGAGTTTGTACTGAGG11280.27706001984614326No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG10900.2677264376172839No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG10470.2571647524635745No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG10360.25446292602890463No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATATCAGGACCA9910.2434099997052553No Hit
ACTTCTACCGTGCCCTGACCCAGTCCACCACCTTCCCACCCATGCTGCAA9670.23751510566597567No Hit
GGAATCCTATGGCTTTCCAACCTTCTACAGCAACAGAGGAGGGGGAGACC9340.22940962636196616No Hit
TCAAATCATCCATTGCTTGGTAGCAGAAAGTCAGTCCCATGGAGACCAAG9340.22940962636196616No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC8660.21270742658400713No Hit
GGGGTCAGAGGCAAGCAGGAGGGCATTCAAGTTATAAAAGAGAAAGCGTG8510.2090231178094574No Hit
ACTTCTACCGTGCCCTGAAGCTGTGTCTACTCATTTGAACAGACCAAGTC8270.20312822377017772No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTAGAC8220.2019001208453278No Hit
CAGCCTCTGGCATTCTGGGAGCTTTATCTGGACCTGGGTCTTCAGTGAAC7870.19330340037137833No Hit
CTGGTGTTGTTGGGCAGTGTATCTCCCCTTTCTTGCGGAGATAGACCAAG7560.18568916223730877No Hit
GCAGCTTCTGCCATCTCTCTGCTACGTAAGAACGTGGGAGTAGACGGAAG7520.18470667989742884No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAAGACCA6980.17144316830904965No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA6940.1704606859691697No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG6850.16825010070443983No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACACACTGA6810.16726761836455992No Hit
GGTCTTCTCTGTTTCAGGGCATGGCAGGCAAGGTGAGCAGACATCGCCTG6640.16309206842007015No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATAGGGGTGGGAGGCTG6230.15302162443630074No Hit
GGAATCCTATGGCTTTCCAACCTGGTTCTACAGCAACAGAGGAGGGGGAG6230.15302162443630074No Hit
TGACAGTTTGACAGTTAAAGGCATGTCGTCATCAGCCGTTACCTGTGTGT5810.1427055598675614No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAAGAACGTGGGAGTAGACGGAA5490.13484570114852185No Hit
TCCCTGCCAGCGAGATACACTGAGGCCTTTGCATCCCTAGACCAAGTCTC5410.13288073646876197No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAGTC5230.12845956593930224No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA5090.12502087774972245No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAGAAGTGGATGAGATGT5080.12477525716475246No Hit
GGTCTTCTCTGTTTCAGGGCATGGTAGAAGGGAGGTGGCTTTAGGTCAGC4990.1225646719000226No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCCA4570.11224860733128322No Hit
TCCACAAACAGAACAAGATGCTACCGTAAATCCTGTTTGAAGGAAAAGGC4520.11102050440643328No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGATGTGGTGGAT4490.11028364265152334No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATTTGCCTCACCTCC4490.11028364265152334No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC4350.10684495446194354No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTTTTCAGTGAAC4200.10316064568739378No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)