FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407132
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA177134.350677421573347No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA162603.993790711611959No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC153653.773960288063822No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA136343.348791055480778No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC120262.9538331548490415No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC115732.8425670298576384No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG114422.81039073322657No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA101332.4888733875008597No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC99392.4412229940166825No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA87382.1462326714677302No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG87292.1440220862030004No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT80331.9730701590638908No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT72251.7746087264081427No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA67911.6680093925311692No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC67211.6508159515832705No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA65031.5972706640598138No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG61511.5108122181503787No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT60131.4769165774245208No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA54811.3462464262204887No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG54691.343298979200849No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC51191.257331774461354No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT49061.2050145898627473No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA47641.1701364667970093No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT45071.107011976459723No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT44491.092765982531464No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC43671.0726250945639253No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG41531.0200622893803484No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA41191.0117111894913688No Hit
AACAGAGAATCTCCATTTTAGCACAGACTTTGTCATATTTTTCCCCTCTG40560.9962370926382599No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA39650.973885619405991No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC36670.9006906850849357No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT34640.8508297063360285No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT34310.8427242270320191No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA31000.761423813406954No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT29310.7199139345470266No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA27370.6722635410628495No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT26520.6513857913404006No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG23340.5732784453199454No Hit
ACTAGATATTCCTTGTCATTATCTGCACCGTATTACCCAAAAGTGAAACA22140.5438039751235472No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT22140.5438039751235472No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC20840.511873299077449No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT18660.45832801155399233No Hit
ACAAGTCAACAACCCCCACATTACGGTAGCAGAGACTTCCATTTTCTACT17750.4359765383217236No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG16880.4146075474293349No Hit
TTCATGGTGTTTTATCCCTCTTGATACGGTAGCAGAGACTTGGATTTGAC16460.4042914828605955No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG15660.38464183606299673No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT14180.34828998948743894No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT13480.33109654853954No Hit
GGCCTCAGTACAAACTCATTAGCACTTGGTCTGGACCTAATTACGAGTGA13040.3202892428008607No Hit
GGCCTCAGTACAAACTCATTAGCCTTGGTCTGGACCTAATTACGAGTGAC11300.27755126101608324No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG10810.265515852352554No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGATAAAGAGGAGCA10140.24905927315956497No Hit
CCATGGGACTGACTTTCTGCTCTTTTCACCCATCTACAGTCCCCCTTGCC10030.24635744672489515No Hit
GTTCAAATGAGTAGACACAGCTTTGCAGCATGGGTGGGAAGGTGGTGGAC9900.2431643791202853No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT9410.23112897045675604No Hit
CCCCCTCCTCTGTTGCTGTAGAAGGTTGGAAAGCCATAGGATTCCTGTAG9380.2303921087018461No Hit
CCATGGGACTGACTTTCTGCTACCAAGCAATGGATGATTTGATGTAGAAC9240.2269534205122663No Hit
CTTGGGCCTGTGTTATCTCCCCTCCCACACTTCACACTCTACTTCAAGCC8710.21393552950885708No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGTA8610.2114793236591572No Hit
GTTCAAATGAGTAGACACAGCTTCAGGGCACGGTAGAAGTTGTAGAACCA8210.2016545002603578No Hit
TCCGTCTACTCCCACGTTCTTACGTAGCAGAGAGATGGCAGAAGCTGCTG7460.18323295638760895No Hit
ATCTCCGCAAGAAAGGGGAGATACACTGCCCAACAACACCAGTGTAGAAC7400.181759232877789No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACTAGGGC7240.17782930351826925No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC6940.1704606859691697No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAAGGC6700.16456579192989007No Hit
GCTGACCTAAAGCCACCTCCCCCAGGCGATGTCTGCTCACCTTGCCTGCC6390.15695155379582051No Hit
AAGACCCAGGTCCAGATGAACCATGTCGTCAGTGTGTAGCAGAGACTTGG6340.1557234508709706No Hit
CCCCCTCCTCTGTTGCTGTAGAACCAGGTTGGAAAGCCATAGGATTCCTG6230.15302162443630074No Hit
CACACACAGGTAACGGCTGATGACGACATGCCTTTAACTGTCAAACTGTC5820.14295118045253136No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA5710.14024935401786154No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG5670.1392668716779816No Hit
AGGGATGCAAAGGCCTCAGTGTATCTCGCTGGCAGGGATGTAGAACCATG5580.1370562864132517No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA5520.13558256290343182No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT5460.1341088393936119No Hit
TCCGTCTACTCCCACGTTCTTACGGTAGCAGAGAGATGGCAGAAGCTGCT5440.13361759822367192No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGACAGAGATTTCATACC5290.12993328944912216No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC5070.12452963657978249No Hit
GCTGACCTAAAGCCACCTCCCTTCTACCATGCCCTGAAACAGAGAAGACC5000.12281029248499259No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTATAGTCCCCCTTGCCGTC4810.11814350137056287No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC4800.11789788078559289No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGAGACAGAGATTT4550.11175736616134324No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCATCTTGTTCTGTTTGTGG4400.10807305738679347No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTACATGGTATTCTT4390.1078274368018235No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGTTGTGGGTTGATTCCACACCCC4290.10537123095212364No Hit
CGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG4280.10512561036715366No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)