FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2191
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG22310.178000912825194No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGATGTAGCGTAT1105.020538566864445No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA1024.655408489274304No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT793.605659516202647No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC773.5143769968051117No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC482.190780465540849No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG482.190780465540849No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA462.0994979461433134No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC411.8712916476494752No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC361.6430853491556368No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC331.5061615700593338No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA331.5061615700593338No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC331.5061615700593338No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG321.460520310360566No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTGTTCATACCAGGACCA291.323596531264263No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTAGAC281.2779552715654952No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC281.2779552715654952No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA271.2323140118667275No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG271.2323140118667275No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT271.2323140118667275No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATTGATAGC271.2323140118667275No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG241.0953902327704246No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT231.0497489730716567No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG210.9584664536741214No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTAGACCAAG210.9584664536741214No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG210.9584664536741214No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT210.9584664536741214No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA200.9128251939753538No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT200.9128251939753538No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG180.8215426745778184No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT180.8215426745778184No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG180.8215426745778184No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA160.730260155180283No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA150.6846188954815152No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCCA150.6846188954815152No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC140.6389776357827476No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT140.6389776357827476No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT140.6389776357827476No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC140.6389776357827476No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT130.5933363760839799No Hit
TCCCTGCCAGCGAGATCTCCTCCATCTTGGAGAAAGGAGAACGCCTCCCT120.5476951163852123No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAGATGTAGCGTATCTCG110.5020538566864445No Hit
CAGTCAGAGGCGCTATGTGTATTACTATAGCTACCTGTTAAAGAATCATC110.5020538566864445No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG100.4564125969876769No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT100.4564125969876769No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA100.4564125969876769No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG100.4564125969876769No Hit
GTGTGGGGGTTGTTGACTTGTAGACCAAGTCTCTGCTACCGTAGATGTAG90.4107713372889092No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC90.4107713372889092No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA90.4107713372889092No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG80.3651300775901415No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG80.3651300775901415No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT80.3651300775901415No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA70.3194888178913738No Hit
TGTGGGGGTTGTTGACTTGTAGACCAAGTCTCTGCTACCGTAGATGTAGC60.27384755819260614No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA60.27384755819260614No Hit
GGGGTCAGAGGCAAGCAGTTCAACTTCTGTAACACCAGATGTTAGTGACA60.27384755819260614No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC60.27384755819260614No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC60.27384755819260614No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCAGAC60.27384755819260614No Hit
TCCAAATACTCCACACGCGAATTTCCTTCCACTCGGATAAGATGCTGAGG50.22820629849383844No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG50.22820629849383844No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA50.22820629849383844No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATATCAGGACCA50.22820629849383844No Hit
GAGAAAGCCCCTCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA50.22820629849383844No Hit
GCAGCTTCTGCCATCTCTCTTCTCCTTTTTCTTCAGCCACAGGCTCCCAG50.22820629849383844No Hit
CAGAGGGGAAAAATATGACAAAGTCTGTGCTAAAATGGAGATTCTCTGTT40.18256503879507074No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG40.18256503879507074No Hit
AGGACAAAATGTTTCGCTTTTGGGTAAATACATTCTTCATACCAGGACCA40.18256503879507074No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCTCAG40.18256503879507074No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTAGGCATCTGCCTCACCTCC40.18256503879507074No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGATGTAGCGTAT40.18256503879507074No Hit
TGACCAATGGCTAAGTGAAGATGGCAATCATGTTGCAGCAATTCACCGTA40.18256503879507074No Hit
ACTTCTACCGTGCCCTGACCCAGTCCACCACCTTCCCACCCATGCTGCAA40.18256503879507074No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT30.13692377909630307No Hit
GGGACAAAATGTTTCACTTTTGGGTAAATACATTGTTCATACCAGGACCA30.13692377909630307No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA30.13692377909630307No Hit
ACTTCACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGATG30.13692377909630307No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTGCTAATGAGTTTGTACTGAG30.13692377909630307No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG30.13692377909630307No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG30.13692377909630307No Hit
CCTCAGAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGCATT30.13692377909630307No Hit
CAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGAGTGGGAGGCTGTCAG30.13692377909630307No Hit
AGTCTTCAGAATGCCAGAGATATACAGGATATGCGAATTAAGAAGAAACA30.13692377909630307No Hit
CTGGTGTTGTTGGGCAGTTCGTGGTGAGGCTCCCCTTTCTTGCGGAGATA30.13692377909630307No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTCCAGCCACAGGCTCCCAG30.13692377909630307No Hit
GCAGTTTCTGCCACTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAGA30.13692377909630307No Hit
CCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA30.13692377909630307No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA30.13692377909630307No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACACTCTTCATACCAGGACCA30.13692377909630307No Hit
TTTCGCTTCCCACAGGTCTCTGCTAGGGGGCTGGGGTTGGGGTGGGGGTG30.13692377909630307No Hit
GCAGCTTCTGCCATCTCTCTCCTCTTTTTTCTTCAGCCACAGGCTCCCAG30.13692377909630307No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)