FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2191
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA2179.904153354632587No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG1145.203103605659516No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC1064.837973528069375No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT813.6969420356001828No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT723.2861706983112735No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC562.5559105431309903No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA492.2364217252396164No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT452.0538566864445458No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA421.9169329073482428No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT401.8256503879507076No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA371.6887266088544046No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC341.5518028297581012No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT331.5061615700593338No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT311.4148790506617983No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA291.323596531264263No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC291.323596531264263No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG281.2779552715654952No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC281.2779552715654952No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA261.1866727521679599No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG261.1866727521679599No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGTA261.1866727521679599No Hit
GTTTCCTCTGGTCCTGGTATGAACAATGTATTTACCCAAAAGTGAAACAT231.0497489730716567No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC221.004107713372889No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA210.9584664536741214No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC210.9584664536741214No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGTGTAGAAC200.9128251939753538No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG190.867183934276586No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG180.8215426745778184No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC170.7759014148790506No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG150.6846188954815152No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA140.6389776357827476No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT140.6389776357827476No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT140.6389776357827476No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT140.6389776357827476No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA140.6389776357827476No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT130.5933363760839799No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC130.5933363760839799Illumina Single End PCR Primer 1 (100% over 21bp)
ACAAGTCAACAACCCCCACAAAATGTTTAATTTAACTGACCTTAAAATTT110.5020538566864445No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG100.4564125969876769No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT100.4564125969876769No Hit
CCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA90.4107713372889092No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC90.4107713372889092No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA90.4107713372889092No Hit
ACAAGTCAACAACCCCCACACTGTAGAACCATGTCGTCAGTGTAGATCTC80.3651300775901415No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG80.3651300775901415No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAGTAATACACATAGCGCCTCTG80.3651300775901415No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT70.3194888178913738No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGAC70.3194888178913738No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT70.3194888178913738No Hit
ACAAGTCAACAACCCCCACATGTAGAACCATGTCGTCAGTGTAGATCTCG70.3194888178913738No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT60.27384755819260614No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTTGTA60.27384755819260614No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT60.27384755819260614No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG60.27384755819260614No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC60.27384755819260614No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA60.27384755819260614No Hit
CCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCAG60.27384755819260614No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG60.27384755819260614No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAACA50.22820629849383844No Hit
CCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG50.22820629849383844No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT50.22820629849383844No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA50.22820629849383844No Hit
CTGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT50.22820629849383844No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCATAGAAAATCTTT50.22820629849383844No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT50.22820629849383844No Hit
CAGCCAGACTGCCTTCCGGGTCACTGCCATGGAGGAGCCGCAGTCAGATC40.18256503879507074No Hit
GTTCAAATGAGTAGACACAGCTTTGCAGCATGGGTGGGAAGGTGGTGGAC40.18256503879507074No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTGAAGAGGAGCA40.18256503879507074No Hit
AACAGAGAATCTCCATTTTAGCACAGACTTTGTCATATTTTTCCCCTCTG40.18256503879507074No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACGGTGAATTGCTGCAACA40.18256503879507074No Hit
CCAACTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC40.18256503879507074No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT40.18256503879507074No Hit
GCTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT30.13692377909630307No Hit
CGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG30.13692377909630307No Hit
ATGAAAACACAACATGAATAAACATCAATATTTGAAATAGAAAATCAAAG30.13692377909630307No Hit
CTCGATATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTAT30.13692377909630307No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATGCACATAGCGCCTCTG30.13692377909630307No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT30.13692377909630307No Hit
TCCGTCTACTCCCACGTTCTAAGGGAGTGACAGAAAGGTAAAGAGGAGCA30.13692377909630307No Hit
CGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA30.13692377909630307No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATCTCA30.13692377909630307No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG30.13692377909630307No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC30.13692377909630307No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATACCTATAATGATCAGGTTCATT30.13692377909630307No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG30.13692377909630307No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAACTGCCCAACAACACCAGT30.13692377909630307No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC30.13692377909630307No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA30.13692377909630307No Hit
CCTCCTGGCTCCTGTCATCTTCTGTCCCTTCCCAGAAAGCCTACCAGGGC30.13692377909630307No Hit
TCCGTCTACTCCCTCGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA30.13692377909630307No Hit
CCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTGA30.13692377909630307No Hit
GCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC30.13692377909630307No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA30.13692377909630307No Hit
TATGAGAACACGCAGAGGGAACTTGGCCTCCTACTGCTGCTTTCAGAGAG30.13692377909630307No Hit
GGCCTCAGTACAAACTCATTAGCACTTGGTCTGGACCTAATTACGAGTGA30.13692377909630307No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)