FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340902
Sequences flagged as poor quality0
Sequence length150
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA256727.53060997002071No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA196785.772333397868009No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC194215.69694516312606No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA110663.2460941854257235No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACAAGCTGTGTCTACTCATTTG107263.1463587775959074No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA92742.7204299182756335No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG74392.1821520554294196No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA64981.9061196472886635No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC60451.7732368833271732No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA59821.7547564989351778No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT59391.742142903239054No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG55831.6377140644525408No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG48091.410669341922312No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACTAGTCTCGTAT45711.3408545564414407No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG45681.33997453813706No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGTAGTGCTAAAATGGA44921.317680741092748No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG44331.300373714439927No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC43291.269866413221395No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT43111.2645863033951106No Hit
AGAAAACGGCATTTTGAGTGTACACTGAGGCATAACTGCACCCTTAGACC40161.1780511701310055No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT39731.1654375744348815No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA39411.1560507125214872No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTAGCTAATGAGTTT33260.9756469601234372No Hit
TGTGCTGTGACTGCTTGTAGAACACAGAGTTGAAAGTCAGGGCAAGACCA31790.9325260632087815No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT31610.927245953382497No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC31230.9160990548603412No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA29780.8735648368152724No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC28770.8439375539011211No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC26430.7752961261594241No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT24970.7324685686795619No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG24910.7307085320708004No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG24520.7192682941138508No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG24220.7104681110700436No Hit
CAGCCTCTGGCATTCTGGGGTCATCAAGAGCAGTCAGAGGACCAGGAGAC22170.6503335269373604No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG21580.6330265002845392No Hit
CAGAGGGGAAAAATATGACAAAGTCTCGTGCTAAAATGGAGATTCTCTGT17780.52155751506298No Hit
GGGGTCAGAGGCAAGCAGTCACAGGGAGATAACACAGGCCCAAGAGACCA16910.4960369842359388No Hit
TGTGCTGTGACTGCTTGTAGAAGTGTACAGAGTTGAAAGTCAGGGCAAGA15880.46582302245220036No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGAGAACGTGGGAGTAGACGGAAGA15390.4514493901473151No Hit
TCATAATGCTTGCTCTGATAGGAACACTGACGACATGGAGTGGGTCCCAT14410.4227021255375445No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAATACGGCC14110.4139019424937372No Hit
GCGTGGACAACCCCCACCACCATCACCACTAATGAGGAGGGGAGATAAGG13250.3886747511014896No Hit
GTAGAGGAGCCGTCAAATCCATGTCGTCATCAAGAGGGATAAAACACCAT13140.3854480173187602No Hit
TCAAATCATCCATTGCTTGGGGTAGCAGAAAGTCAGTCCCATGGAGACCA12910.37870121031850795No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAGACAAATGTATGATGCACATC12440.36491425688320983No Hit
CTGGTGTTGTTGGGCAGTGTATCTCCCCTTTCTTGCGGAGATAGACCAAG12330.3616875231004805No Hit
GGTCTTCTCTGTTTCAGGGCATACGGTAGGAGGTGGCTTTAGGTCAGCAG12180.35728743157857684No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC12120.3555273949698154No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAAGAACGTGGGAGTAGACGGAA12090.35464737666543467No Hit
GGAATCCTATGGCTTTCCAACCCATGGTTCTACAGCAACAGAGGAGGGGG11840.3473138907955952No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC11630.34115376266493014No Hit
TCTGTATCAGGCAAAGTCATAGAACCAGGAGCCATTGTCTTTGAGGCAGA9660.2833658940105954No Hit
TCCCTGCCAGCGAGATACACTGAGGCCTTTGCATCCCTAGACCAAGTCTC9610.28189919683662756No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAATCTCTGCTACCGTATTC9050.2654721884881872No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT8640.2534452716616506No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT8600.2522719139224762No Hit
GCGTGGACAACCCCCACCATGCAGTTCCCTCATCTGGGAGGGGAGATAAG8590.2519785744876827No Hit
GGTCTTCTCTGTTTCAGGGCATGGTAGGGAGGTGGCTTTAGGTCAGCAGA8050.23613824500882952No Hit
CAGCCTCTGGCATTCTGGCCGTATTGAGAGCAGTCAGAGGACCAGGAGAC8020.2352582267044488No Hit
GGGGTCAGAGGCAAGCAGGGAGATAACACAGGCCCAAGAGACCAAGTCTC7930.2326181717913066No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT7800.22880475913899012No Hit
GGGGTCAGAGGCAAGCAGGAGGAGATAACACAGGCCCAAGAGACCAAGTC7650.22440466761708644No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTACTA7540.22117793383435708No Hit
TCAAATCATCCATTGCTTGGTACGGTTAGCAGAAAGTCAGTCCCATGGAG7170.21032437474699472No Hit
TCCACAAACAGAACAAGATGCTACCGTAAATCCTGTTTGAAGGAAAAGGC7000.20533760435550394No Hit
GGTCTTCTCTGTTTCAGGGCATGGTAGAGGAGGTGGCTTTAGGTCAGCAG6900.20240421000756814No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTAGAC6410.1880305777026829No Hit
TCTTAAATGGCTACGACCCAGACTGACGACATGGTTCTACATCTTAAATG6210.18216378900681135No Hit
GCGTGGACAACCCCCACCACCATCGCCACTAATGAGGAGGGGAGATAAGG5910.173363605963004No Hit
GTAGAGGAGCCGTCAAATCCATGAAAGACCAAGTCTCTGCTACCGTACTA5850.17160356935424256No Hit
TCTGTATCAGGCAAAGTCATAGAACCATGGGAGCCATTGTCTTTGAGGCA5780.16955019331068752No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACTAGTCTCGTATCTCG5680.16661679896275175No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC5320.156056579310183No Hit
TCCCTGCCAGCGAGATGAGGCCTTTGCATCCCTAGACCAAGTCTCTGCTA5240.15370986383183438No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAC5210.15282984552745363No Hit
ATACGGCCAGGCATTGAAGTCTCTGCTACCGTACACTGACGACAGGGGCC4840.14197628644009128No Hit
CCCCTGCTCCTATAGCCAAGTCGTCAGTGTGATGCCGACGAGTACCTCAT4790.14050958926612342No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTACCGTACTAGTCT4780.14021624983132983No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC4670.1369895160486005No Hit
GGAATCCTATGGCTTTCCAACCCACTGACGTCATGGTTCTACAGCAACAG4400.12906935130917388No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC4360.1278959935699996No Hit
GGAAACCGTAGCTGCCCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTC4290.12584261752644454No Hit
TCAAATCATCCATTGCTTGGTACGGAAGCAGAAAGTCAGTCCCATGGAGA4240.12437592035247666No Hit
TCATAATGCTTGCTCTGATAGGACACTGACGACATGGAGTGGGTCCCATC4060.11909581052619228No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT4050.1188024710913987No Hit
TGTGCTGTGACTGCTTGTAGAACCAACAGAGTTGAAAGTCAGGGCAAGAC4050.1188024710913987No Hit
GCGTGGACAACCCCCACCACTGACTCTGATCCAGAAGACCAAGTCTCTGC3990.11704243448263724No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTAGACCAAG3930.11528239787387577No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG3900.11440237956949506No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA3890.11410904013470147No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA3880.11381570069990789No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA3750.11000228804759139No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTAGCTACCTGTTAAAGAATCAT3420.10032208669940335No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)