FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340902
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC221596.500108535590873No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA193095.664091146429179No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA124913.664102880006571No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA122093.581381159394782No Hit
GTTCAAATGAGTAGACACAGCTTGTCTTCTCTGTTTCAGGGCACGGTAGA106933.136678576247719No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA106473.1231849622472145No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG69222.03049556764114No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA64431.8899859783750168No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTATCTCTA61141.7934773043279302No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA60211.7661967368921272No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC58431.7139823174988706No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT55651.6324339546262563No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT51091.498671172360385No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT48121.4115493602266926No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG46041.3505347577896287No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT45361.3305876762236655No Hit
AACAGAGAATCTCCATTTTAGCACTACGGTAGCAGAGACTTTGTCATATT45251.327360942440936No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG42781.2549061020469225No Hit
AAGGGTGCAGTTATGCCTCAGTGTACACTCAAAATGCCGTTTTCTTGTAG39861.169250987087198No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT39311.1531173181735515No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA37241.0923960551712808No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT35981.0554352863872902No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC33800.9914872896022904No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT32360.9492464109920153No Hit
GGCCTCAGTACAAACTCATTAGCTAGCAGAGACTTGGTCTGGACCTAATT32320.948073053252841No Hit
TGCCCTGACTTTCAACTCTGTGTTCTACAAGCAGTCACAGCACATGTAGA31460.9228458618605934No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT29880.8764982311632082No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT29310.8597778833799743No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA28560.837777425770456No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG24840.7286551560272454No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT24830.7283618165924518No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG24280.712228147678805No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG23380.6858275985473832No Hit
CCTGGTCCTCTGACTGCTCTTGATGACCCCAGAATGCCAGAGGCTGTGTA22040.6465201142850439No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA21450.6292130876322227No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG21430.6286264087626355No Hit
TCCGTCTACTCCCACGTTCTAGGAGAGTGACAGAAAGGTAAAGAGGAGCA19530.572891916151856No Hit
AACAGAGAATCTCCATTTTAGCACGAGACTTTGTCATATTTTTCCCCTCT17150.5030771306709847No Hit
CTTGGGCCTGTGTTATCTCCCTGTGACTGCTTGCCTCTGACCCCTGTAGA16720.4904635349748608No Hit
TGCCCTGACTTTCAACTCTGTACACTTCTACAAGCAGTCACAGCACATGT15570.45672949997359946No Hit
TCCGTCTACTCCCACGTTCTCGGTAGCAGAGAGATGGCAGAAGCTGCTGT15340.4499826929733472No Hit
CTGATGGGACCCACTCCATGTCGTCAGTGTTCCTATCAGAGCAAGCATTA14030.4115552270153886No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC13590.39864829188447115No Hit
GGCTCCTTATCTCCCCTCCTCATTAGTGGTGATGGTGGTGGGGGTTGTCC13140.3854480173187602No Hit
TTCATGGTGTTTTATCCCTCTTGATGACGACATGGATTTGACGGCTCCTC12980.380754586362063No Hit
CCATGGGACTGACTTTCTGCTACCCCAAGCAATGGATGATTTGATGTAGA12930.3792878891880951No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG12770.37459445823139786No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC12640.37078104557908137No Hit
ATCTCCGCAAGAAAGGGGAGATACACTGCCCAACAACACCAGTGTAGAAC12220.35846078931775116No Hit
GCTGACCTAAAGCCACCTCCTACCGTATGCCCTGAAACAGAGAAGACCTG12080.3543540372306411No Hit
TCCGTCTACTCCCACGTTCTTACGGTAGCAGAGAGATGGCAGAAGCTGCT11990.35171398231749884No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGGGTTGGAAAGCCATAGGATTCC11810.3464338724912145No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA10240.3003795812286229No Hit
AGGGATGCAAAGGCCTCAGTGTATCTCGCTGGCAGGGATGTAGAACCATG9660.2833658940105954No Hit
GCCTCAAAGACAATGGCTCCTGGTTCTATGACTTTGCCTGATACAGATGT9660.2833658940105954No Hit
AAGACCCAGGTCCAGATGAATACGGTAGCAGAGATTTGGTCTAAGACCCA8950.26253879414025144No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC8650.25373861109644413No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG8610.2525652533572698No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGTGC8460.2481651618353662No Hit
GGCTCCTTATCTCCCCTCCCAGATGAGGGAACTGCATGGTGGGGGTTGTC8400.2464051252266047No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA8090.23731160274800384No Hit
GCTGACCTAAAGCCACCTCCCTACCATGCCCTGAAACAGAGAAGACCTGT8050.23613824500882952No Hit
CCTGGTCCTCTGACTGCTCTCAATACGGCCAGAATGCCAGAGGCTGTGTA7990.23437820840006807No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTTAGGCTTC7900.23173815348692586No Hit
CTTGGGCCTGTGTTATCTCCCTGCTTGCCTCTGACCCCTGTAGAACCATG7850.23027145631295798No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC7800.22880475913899012No Hit
CTTGGGCCTGTGTTATCTCCTCCTGCTTGCCTCTGACCCCTGTAGAACCA7510.22029791552997635No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT7400.217071181747247No Hit
CCATGGGACTGACTTTCTGCTAACCGTACCAAGCAATGGATGATTTGATG7220.2117910719209626No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCATCTTGTTCTGTTTGTGG6870.20152419170318742No Hit
GCTGACCTAAAGCCACCTCCTCTACCATGCCCTGAAACAGAGAAGACCTG6850.20093751283360028No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGTA6290.18451050448515996No Hit
TTCATGGATTTGACGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA5970.1751236425717655No Hit
GCCTCAAAGACAATGGCTCCCATGGTTCTATGACTTTGCCTGATACAGAT5800.17013687218027468No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC5770.16925685387589395Illumina Single End PCR Primer 1 (100% over 21bp)
GGCTCCTTATCTCCCCTCCTCATTAGTGGCGATGGTGGTGGGGGTTGTCC5740.16837683557151323No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGCGACAGAAAGGTAAAGAGGAGCA5730.16808349613671966No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA5680.16661679896275175No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACGGTAGCAGAGACTTCAATGCCTGG5280.15488322157100867No Hit
AGGGATGCAAAGGCCTCATCTCGCTGGCAGGGATGTAGAACCATGTCGTC5240.15370986383183438No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA5160.15136314835348574No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC4970.14578969909240777No Hit
GGACCTAATTACGAGTGACACTGTAGAACCATGTCGTCAGTGTAGATCTC4750.13933623152694907No Hit
ATGAGGTACTCGTCGGCATCACACTGACGACTTGGCTATAGGAGCAGGGG4750.13933623152694907No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGACGTCAGTGGGTTGGAAAGCCA4530.1328827639614904No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA4350.12760265413520602No Hit
CCATGGGACTGACTTTCTGCTTCCGTACCAAGCAATGGATGATTTGATGT4230.1240825809176831No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA4190.12290922317850879No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGGGCAGCTACGGTTT4170.12232254430892163No Hit
TGCCCTGACTTTCAACTCTGTTGGTTCTACAAGCAGTCACAGCACATGTA4050.1188024710913987No Hit
TCTGGATCAGAGTCAGTGGTGGGGGTTGTCCACGCTGTAGAACCATGTCG3920.11498905843908219No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGTGTAGAAC3880.11381570069990789No Hit
CTGATGGGACCCACTCCATGTCGTCAGTGTCCTATCAGAGCAAGCATTAT3860.11322902183032073No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT3850.11293568239552716No Hit
CAGCCAGACTGCCTTCCGTATACGGTAGCAGAGACCTGTGGGAAGCGAAA3800.11146898522155926No Hit
CCTCCTGGCCCCTGTCTCAGTGTACTTCAATGCCTGGCCGTATTGTAGAA3790.1111756457867657No Hit
CCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA3420.10032208669940335No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)