FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537024
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT235164.378947682040281No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA208613.884556369920152No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC193593.6048668215945656No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA190083.5395066142295315No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC145702.71310034560839No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG138562.5801453938743895No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG122832.2872348349422No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC118242.2017637945417707No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA117512.18817036110118No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT114422.1306310332499105No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA108332.017228280300322No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT104061.9377160052437135No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG100021.8624865927779763No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC100021.8624865927779763No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC98321.8308306518889286No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA97481.8151888928613993No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG96941.8051334763437017No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG96591.7986160767488977No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA89631.6690129305207961No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT72611.3520810987963294No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT71611.3334599570968895No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG70481.3124180669765224No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG67841.2632582528900012No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT66491.2381197115957574No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT56801.057680848528185No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC56021.043156358002622No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA48610.9051736980097723No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT43480.8096472410916458No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT43370.8075989155047074No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC42850.7979159218209986No Hit
TCCCTGCCAGCGAGATCTCCTCCATCTTGGAGAAAGGAGAACGCCTCCCT42710.7953089619830772No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG42120.7843224883804076No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC39610.7375834227148135No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG36930.6876787629603146No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC35890.6683127755928971No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG35280.6569538791562388No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA34800.6480157311405077No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG33950.6321877606959838No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC32520.6055595280657848No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC31070.5785588726015969No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA29040.540757954951734No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACTGTAGTGCGAT28820.5366613037778573No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG28500.5307025384340365No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGGTGCTAAAATGGAGATTCTCT27150.5055639971397926No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA26460.4927154093671791No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG25330.4716735192468121No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA23530.43815546418782025No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT23080.4297759504230723No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA23030.4288448933381004No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG23020.42865868192110596No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA21200.3947682040281254No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT18910.35212578953640805No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC18570.3457946013585985No Hit
GACCCAAAACCCAAAATGGCAGGGAAGGGAGAGATGGGGGTGGGAGGCTG16170.3011038612799428No Hit
TGACCAATGGCTAAGTGAAGATGACAGAGACTTGTTTTTCCTTTCCTCTT13410.24970951018948875No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG13070.24337832201167917No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC11570.21544660946251937No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCGGCTCGTGGTG11170.2079981527827434No Hit
CAGAGGGGAAAAATATGACAAAGTCTCGTGCTAAAATGGAGATTCTCTGT11020.20520498152782743No Hit
CAGAGGGGAAAAATATGACAAATACGGTGCTAAAATGGAGATTCTCTGTT10660.19850137051602906No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATAGGGGTGGAAGGCTG10440.1944047193421523No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG10280.19142533667024192No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGTGTGGGGGTTGTTGACTTG10050.18714247407937076No Hit
ATACGGCCAGGCATTGAAGTCATGACGACAGGGGCCAGGAGGAGACCAAG9660.1798802288165892No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAAGACCA9600.1787629603146228No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGAGAGGCTG8780.16349362412108212No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGAGGGTGGGAGGCTG8370.15585895602431177No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGATCTTC8180.1523209391014182No Hit
AGAAAACGGCATTTTGAGTGTCTGCTACCGTAGGGTGAGGCATAACTGCA7940.14785186509355264No Hit
TCAAATCATCCATTGCTTGGTACCGGTAGCAGAAAGTCAGTCCCATGGAG7460.1389137170778215No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA7320.1363067572398999No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGATGGGAGGCTG7250.13500327732093909No Hit
AGGACAAAATGTTTCACTTTTGGGTAATACGGTGCAGATAATGACAAGGA6840.12736860922416876No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACTA6570.12234090096531998No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGGCTAATGAGTTTGTACTGAGG6370.11861667262543202No Hit
GAGAAAGCCCCCCTACTGCTCACCTGGAGGGCCACTGACAACCACCCTTA6360.11843046120843762No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGAACCA6190.11526486711953284No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGAACCTGGGTCTTCAGTGAAC5710.10632671910380169No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)