FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537024
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC191213.560548504349899No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA185053.445842271481349No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA181543.380482064116315No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA153492.8581590394470267No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG144632.6931757239899894No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG136942.5499791443212967No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC121012.2533443570492198No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC114692.135658741508759No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC114462.131375878917888No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA106881.990227624836134No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC106251.978496305565487No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA104751.9505645930163271No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT100511.8716109522107018No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG95311.7747810153736145No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA91751.7084897509236086No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT91271.6995516029078777No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA89321.6632403765939698No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT87421.627860207365034No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT87061.6211565963532355No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT81281.5135263973304731No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG74431.38597157668931No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA72431.3487292932904302No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC69351.2913761768561554No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT66961.2468716481944941No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT64691.2046016565367654No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT63881.1895185317602193No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC52070.9696028482898343No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC49260.9172774401144083No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA48270.8988425098319628No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA47010.8753798712906685No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT45880.8543379811703016No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT44900.8360892623048505No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT44140.8219371946132762No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA42650.7941916934811107No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG35910.668685198426886No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC35360.658443570492194No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA32120.5981110713860088No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT31810.5923385174591824No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC31790.5919660946251937No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC31740.5910350375402216No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG29180.5433649147896555No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA28780.5359164581098796No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT28520.5310749612680253No Hit
AACAGAGAATCTCCATTTTAGCACCAGAGACTTTGTCATATTTTTCCCCT26390.4914119294482183No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT26370.4910395066142295No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT23580.4390865212727923No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT23160.43126564175902754No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG22450.41804463115242524No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG21640.4029615063758789No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT21330.39718895244905256No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT18940.3526844237873913No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG12880.2398403050887856No Hit
TCCAGGAAGAGGAAAGGAAAAACAAGTCTCTGTCATCTTCACTTAGCCAT12520.23313669407698726No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCTTCTG11860.22084674055535694No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT11730.21842599213442973No Hit
AACAGAGAATCTCCATTTTAGCACGAGACTTTGTCATATTTTTCCCCTCT10620.1977565248480515No Hit
AACAGAGAATCTCCATTTTAGCACCGTATTTGTCATATTTTTCCCCTCTG10550.1964530449290907No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGAC10450.1945909307591467No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGAC10410.1938460850911691No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG10230.19049427958526993No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACTAGGGC9850.18341824573948276No Hit
CCTCCTGGCCCCTGTCGTCATGACTTCAATGCCTGGCCGTATTGTAGAAC9790.18230097723751637No Hit
CCATGGGACTGACTTTCTGCTCTTTTCACCCATCTACAGTCCCCCTTGCC9490.17671463472768442No Hit
ACAAGTCAACAACCCCCACACACTGACTTCCATTTTCTACTTTTTCTGAG9420.17541115480872363No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGACAGAGATTTCATACC9380.17466630914074605No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT9150.17038344654987486No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTCATCACCTTTCCTTGCCTCT9140.1701972351328805No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGTCCAAAATCTGTGATCTTGAC9090.16926617804790847No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGATTTTTGTAAT8570.15958318436419974No Hit
AAGGGTGCAGTTATGCCTCACCCTACGGTAGCAGACACTCAAAATGCCGT8100.150831247765463No Hit
CCATGGGACTGACTTTCTGCTACCGGTACCAAGCAATGGATGATTTGATG7400.13779644857585507No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA7250.13500327732093909No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG7250.13500327732093909No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC6930.12904451197711836No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGAGACAGAGATTT6730.12532028363723036No Hit
ACTAGATATTCCTTGTCATTATCTGCACCGTATTACCCAAAAGTGAAACA6380.11880288404242641No Hit
GGCCTCAGTACAAACTCATTAGCCTTGGTCTGGACCTAATTACGAGTGAC6230.11600971278751043No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT6200.11545107853652722No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT5700.1061405076868073No Hit
CGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG5660.1053956620188297No Hit
TTCTCTTCCGCACCCAGTAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC5570.1037197592658801No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTATAGTCCCCCTTGCCGTC5490.10223006792992491No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT5420.10092658801096413No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)