FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476991
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA298906.266365612768375No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA269485.649582486881304No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT246445.1665545052212725No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC198644.1644391613258955No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC123702.5933403355618867No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC118982.4943866865412554No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT112062.3493105739940585No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG109922.3044459958363994No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA107612.2560174091335057No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG103372.167126843064125No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT98632.067753898920525No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG91791.924354966865203No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC90781.9031805631552796No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA86911.8220469568608213No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA86631.8161768251392585No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC82501.7295923822462058No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT82101.7212064797868305No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA70701.4822082596946273No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG65171.3662731581937604No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT63621.3337777861636801No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG59201.2411135639875805No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT58021.2163751517324226No Hit
GTAGAGGAGCCGTCAAATCCAAGTCTAGGAGCCGTCAAGAGGGATAAAAC56311.1805254187185923No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT55191.1570448918323406No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGTAGTGCTAAAATGGA48891.0249669280971758No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG44790.9390114278885765No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC44490.9327220010440448No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC43560.9132247778259966No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA41670.8736013887054472No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA36800.7715030262625501No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG28930.6065103953743362No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCTTGGGGGCAGCTCGTGGTG28660.6008499112142577No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG26900.5639519403930053No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG26390.5532599147573014No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC23280.48805952313565665No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG22620.474222784077687No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA21910.4593378072122954No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG21770.456402741351514No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA21600.4528387328062794No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACTCAGCAGTGAT20320.4260038449362776No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC20080.4209723034606523No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA18450.38679975093869695No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG15860.33250103251424035No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG15510.3251633678622867No Hit
AGGACAAAATGTTTCACTTTTGGGTAGAAGCCCTGATGATGGGCTGTGCA15090.3163581702799424No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGGCTAATGAGTTTGTA14500.3039889641523635No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT14360.301053898291582No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG13720.28763645435658114No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACCAAGCTGTGTCTACTCATTT13700.2872171592336124No Hit
TGAGCAAGAGGCTTTGGAGTTCCCTCTCCTAGTCAAAGAAAGGGGTGACA13410.2811373799505651No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA13300.2788312567742368No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACAAGCTGTGTCTACTCATTTG13250.2777830189668149No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA12880.27002605919189254No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT12210.2559796725724385No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC11270.23627280179290594No Hit
GCAACAGATAACTCAGATTGCCTTGTTCTACATCATACCAGGACCAGAGG11250.23585350666993718No Hit
GGGGTCAGAGGCAAGCAGGAGATAACACAGGCCCAAGAGACCAAGTCTCT10650.22327465298087384No Hit
AGGACAAAATGTTTCACTTTTGGGTAAAAGCCCTGATGATGGGCTGTGCA10270.2153080456444671No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG10160.2130019224681388No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTACGGCTAATGAGTTTGTACT9650.202309896832435No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACCAGGGACGAACTGGTGTAATG9460.1983265931642316No Hit
ACTTCTACCGTGCCCTGACCGTAAGCTGTGTCTACTCATTTGAACAGACC9340.19581082242641892No Hit
TCTTAAATGGCTACGACCCAGCTACCTGTTAAAGAATCATCTGGAAGACC8350.17505571383946447No Hit
ATACGGCCAGGCATTGAAGTCACAGAGTTGAACATTCCCTTTGAGAGAGC7920.16604086869563575No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC7850.16457333576524505No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA7340.15388131012954123No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG7280.1526234247606349No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC7250.15199448207618174No Hit
CTGGTGTTGTTGGGCAGTCATGGTTCTCCCCTTTCTTGCGGAGATAGACC6980.14633399791610324No Hit
AGATGAGTCATATTTGTGGGTTTTCACCACTGACTCTGATCCAGAAGACC6960.14591470279313445No Hit
GGAATCCTATGGCTTTCCAACCGTTCTACAGCAACAGAGGAGGGGGAGAC6610.13857703814118086No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACGGAGGG5990.12557888932914876No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGTGTGGGGGTTGTTGACTTG5670.11887016736164832No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTACTC5560.11656404418532007No Hit
GTGTCACTCGTAATTAGGTCCAGACGCTAATGAGTTTGTACTGAGGCCAG5280.11069391246375716No Hit
TCCACAAACAGAACAAGATGCTACCGTAAATCCTGTTTGAAGGAAAAGGC5200.10901673197188207No Hit
GGAATCCTATGGCTTTCCAACCCACTGACGACATGGTTCTACAGCAACAG5190.10880708441039769No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTACAACAATAATGAGTC5150.10796849416446012No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCGATAACAATAATGAGTC5050.10587201854961624No Hit
TCAAATCATCCATTGCTTGGTACCGGTAGCAGAAAGTCAGTCCCATGGAG4940.10356589537328796No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCGTAGCTACCTGTTAAAGA4910.1029369526888348No Hit
GGTCTTCTCTGTTTCAGGGCATCACCCGCTGGAGGTGGCTTTAGGTCAGC4900.10272730512735041No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT4820.1010501246354753No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)