FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476991
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA283295.939105769291245No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC241185.056279887880484No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA191334.011186793880807No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA151573.1776280894188784No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG119442.504030474369537No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC110762.3220563910010883No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA108172.2677576725766313No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA105192.2052826992542838No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC105052.202347633393502No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG98272.0602065867070865No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC92961.9488837315588765No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA91891.926451442480047No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC91791.924354966865203No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT90221.8914402997121538No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG86591.8153382348933207No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT77291.6203660027128395No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA75471.5822101465226805No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT75291.5784364904159618No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA70541.4788538987108772No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC64571.3536943045046972No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA61891.297508758026881No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT59981.2574660737833627No Hit
TTCATGGTGTTTTATCCCTCTTGACGGCTCCTAGACTTGGATTTGACGGC53571.1230819868718698No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT52761.1061005343916341No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA49081.0289502317653794No Hit
AACAGAGAATCTCCATTTTAGCACTACGGTAGCAGAGACTTTGTCATATT48431.015323140268894No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA44270.9281097546913882No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT43770.9176273766171689No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA42870.898759096083574No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC42010.8807294057959165No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT41630.8727627984595097No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA36490.765003951856534No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT30270.6346031686132443No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT27390.5742246709057404No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT26350.5524213245113639No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC25580.536278462277066No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG25480.5341819866622222No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC25280.5299890354325344No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG23280.48805952313565665No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT21430.44927472426104476No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG20370.42705208274369955No Hit
TCATTACACCAGTTCGTCCCTTTCCAACTTTACAGTGAATTGCTGCAACA18990.39812071925885395No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT16770.35157896060931965No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT16090.33732292642838124No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT15580.3266309007926774No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC15060.3157292275954892No Hit
ACTAGATATTCCTTGTCATTATCTGCACAGCCCATCATCAGGGCTTCTAC14820.31069768611986387No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT14310.3000056604841601No Hit
GGCCTCAGTACAAACTCATTAGCCAGAGACTTGGTCTGGACCTAATTACG14220.29811883243080056No Hit
GTTCAAATGAGTAGACACAGCTTGGTCTTCTCTGTTTCAGGGCACGGTAG13650.28616892142619044No Hit
GGTCTTTGCCTGCTGAGAGTGACCCGATTTTCCAGGTGCCGTCTGTCACC13300.2788312567742368No Hit
GTTCAAATGAGTAGACACAGCTTGTCTTCTCTGTTTCAGGGCACGGTAGA13010.27275147749118955No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAAAGACAGAGATTTCA12670.2656234604007204No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG12180.2553507298879853No Hit
GTTTCCTCTGGTCCTGGTATGATGTAGAACAAGGCAATCTGAGTTATCTG11570.24256222863743762No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG11290.23669209691587473No Hit
CTTGGGCCTGTGTTATCTCCTGCTTGCCTCTGACCCCTGTAGAACCATGT10540.22096852980454557No Hit
ACTAGATATTCCTTGTCATTATCTGCACAGCCCATCATCAGGGCTTTTAC10270.2153080456444671No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG10110.21195368466071685No Hit
GGCCTCAGTACAAACTCATTAGCCGTACTTGGTCTGGACCTAATTACGAG9650.202309896832435No Hit
GTTCAAATGAGTAGACACAGCTTACGGTCAGGGCACGGTAGAAGTTGTAG9380.19664941267235647No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC9330.19560117486493458No Hit
TCATTACACCAGTTCGTCCCTGGTGGTAAAAAGATAACCTCAGAATAAGA8980.18826351021298096No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA8420.1765232467698552No Hit
TCCAGATGATTCTTTAACAGGTAGCTGGGTCGTAGCCATTTAAGATGTAG8270.17337853334758938No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGACAGAGATTTCATACC7780.16310580283485432No Hit
CCTGGTCCTCTGACTGCTCTCTCAAAGGGAATGTTCAACTCTGTGACTTC7580.15891285160516655No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC7050.14780153084649397No Hit
TCTGGATCAGAGTCAGTGGTGAAAACCCACAAATATGACTCATCTTGTAG6940.14549540767016567No Hit
ATCTCCGCAAGAAAGGGGAGAACCATGACTGCCCAACAACACCAGTGTAG6870.14402787473977496No Hit
CCCCCTCCTCTGTTGCTGTAGAACGGTTGGAAAGCCATAGGATTCCTGTA6620.13878668570266525No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG5670.11887016736164832No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA5610.117612281992742No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCCA5460.11446756857047619No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGGGTTGGAAAGCCA5440.1140482734475074No Hit
ACAAGTCAACAACCCCCACACACTGACTTCCATTTTCTACTTTTTCTGAG5370.11258074051711667No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGAGACAGAGATTT5340.11195179783266351No Hit
GGCCTCAGTACAAACTCATTAGCGTCTGGACCTAATTACGAGTGACACTG5250.11006496977930401No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCATCTTGTTCTGTTTGTGG5120.10733955148000696No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGAC4980.10440448561922552No Hit
CGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG4970.10419483805774114No Hit
CCATGGGACTGACTTTCTGCTACCGGTACCAAGCAATGGATGATTTGATG4880.10230801000438164No Hit
GCTGACCTAAAGCCACCTCCAGCGGGTGATGCCCTGAAACAGAGAAGACC4860.10188871488141285No Hit
CTCGATAATCTGGATGACTCATTATTGTTGTAGCACAACTAAAAGAAAAG4840.10146941975844408No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT4780.10021153438953775No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)