FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC83.418803418803419No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC83.418803418803419No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA52.1367521367521367No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA52.1367521367521367No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT52.1367521367521367No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG41.7094017094017095No Hit
TCCCTCTTCCCCCTCTTGGTGGGACTCTTGTAGGCCCCCGATAGAGTTTT41.7094017094017095No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC41.7094017094017095No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT41.7094017094017095No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG41.7094017094017095No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT31.282051282051282No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC31.282051282051282No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT31.282051282051282No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT31.282051282051282No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC31.282051282051282No Hit
CAAATACCAGTGAACTTAAAGAATTCGACCCTTAGAAACTACATGTAGAA31.282051282051282No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA31.282051282051282No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC31.282051282051282No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT31.282051282051282No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG31.282051282051282No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT20.8547008547008548No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA20.8547008547008548No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA20.8547008547008548No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG20.8547008547008548No Hit
CCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAGA20.8547008547008548No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT20.8547008547008548No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC20.8547008547008548No Hit
GTTCTGGAGTACGTATAGCAGTATTTTCTTCTCTTGTGAGCTGGTCTGAA20.8547008547008548No Hit
ACCAGCGGTAGAAATTGTACATGACCACGGCGGCAATAAAGGTCTTTCCC20.8547008547008548No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT20.8547008547008548No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT20.8547008547008548No Hit
CTTACCAATACTCCATCCACAGATGAAACTTTCTCCCAAGTTAACCAAGC20.8547008547008548No Hit
CCTGGAGTCGATTGATTAGAGCCTAGTCCAGGAGAATGAATTGACACTAA20.8547008547008548No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCATAGAAAATCTTT20.8547008547008548No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT20.8547008547008548No Hit
CTGATGGGACCCACTCCATCGAGATTCCACTGTAGCTAGACCAAAATCAC20.8547008547008548No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG20.8547008547008548No Hit
ACTTTCAAGTTTCCCCACCCTTTACACAGTTTCCCCGCGGAAAACGCCGC20.8547008547008548No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG10.4273504273504274No Hit
CAGCCAGACTGCCTTCCGGGTCACTGCCATGGAGGAGCCGCAGTCAGATC10.4273504273504274No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC10.4273504273504274No Hit
CCATACAGCAAAGCAGAAACTCACATTGAGGATTTCCTTGTTGGCTTTCG10.4273504273504274No Hit
CTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTTC10.4273504273504274No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGGCTGTACCACCATCCAC10.4273504273504274No Hit
GGCTCCTTATCTCCCCTCCCGTATCTCCCTTCCCTGATTACCTTTGCGAT10.4273504273504274No Hit
TCTGAGCACCAGTGGAGATTTTGTTAAGCAAACGGTGCCCTCAGAAAATA10.4273504273504274No Hit
TGTGAGCTGGTCTGAATGTTCGTTACTTTTAAACAATACTTTGGAAAAGA10.4273504273504274No Hit
TGATGACTAGTTAATTTCTTGCAGATGTAAAAAAGCATTTGTGAGCTGCA10.4273504273504274No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA10.4273504273504274No Hit
AGGGGTGGTAAATGTTGACCTATGTGAATGCGTGCTACATTCATGTAGAA10.4273504273504274No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA10.4273504273504274No Hit
GAAAGCCAGGGAGTTGGTCTGAGTGACAAGGAATTGGTTTCAGATGATGA10.4273504273504274No Hit
CGAGTCACACATTTGACTTACTTAAACACATCTGCACTTATTTTACCAGT10.4273504273504274No Hit
GATGTTTTGAGATTTTCAGTTTGTCTACATAAATTATCACTTAAGAGCTT10.4273504273504274No Hit
ATCTCCGCAAGAAAGGGGTGCCTCACCACGAGCTGCCCCCAGGGAGCACT10.4273504273504274No Hit
GGATTACTCTTAGATTTGTGTTTTGGTTGAATTGTACCTTTCTTGAAGGT10.4273504273504274No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA10.4273504273504274No Hit
ATCTTACCTTCAACAGAATGACAGGATTTATGCAGATACATTGGACCACT10.4273504273504274No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA10.4273504273504274No Hit
TGGGAGGTGAGAGTTTAATAAAATAGTCAAGGACACATAGCATTAACTGA10.4273504273504274No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT10.4273504273504274No Hit
GTTCAAATGAGTAGACGCAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA10.4273504273504274No Hit
TGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATA10.4273504273504274No Hit
GGCCTCAGTACAATCATTAGCATCAGGATTATGACTCACCGTAGCTCCAG10.4273504273504274No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGATAATCTACTGGGACG10.4273504273504274No Hit
AACCCCAAGGGACTAATTCATGGTTGTTCCAAAGATAATAGAAATGACAC10.4273504273504274No Hit
TCTCCCTATAAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT10.4273504273504274No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAGACATACT10.4273504273504274No Hit
GATGGGGAGTCTGAATCAAATGCCAAAGTAGCTGATGTATTGGACGTTCT10.4273504273504274No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT10.4273504273504274No Hit
AAGGGTGCATGCCTCAGATTCATTTTTATCACCTTTCCTTACCTCTTTCC10.4273504273504274No Hit
ATGAGGTACTCGTCGGCATCTATGGTAGCAGAGACTTGGCTATAGGAGCA10.4273504273504274No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG10.4273504273504274No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTTTTCTAAACTGA10.4273504273504274No Hit
TCTCCCAATGAAGTGAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTTG10.4273504273504274No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCACACAAATAAGAAAACATACT10.4273504273504274No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT10.4273504273504274No Hit
CTTGGGCCTGTGTTATCTCCTACGTTGGCTCTGACTGTACCACCATCCAC10.4273504273504274No Hit
GCCCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCAA10.4273504273504274No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT10.4273504273504274No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTACTCATAACTTTTTTTTTTTTT10.4273504273504274No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTACAGATAATACAAG10.4273504273504274No Hit
CAATGATGAACACTGCGGGCTTCCAGACAATGTTAACACGCTGAATGCCA10.4273504273504274No Hit
ATGATCCATGGACATTTGACTGGTATTTCCACTAGAAGGAATATCACATC10.4273504273504274No Hit
AAGACAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGCGT10.4273504273504274No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTATCAGGGC10.4273504273504274No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA10.4273504273504274No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC10.4273504273504274No Hit
TTCATGGTGTTTTATCCCTCTGATAAAAAAAAATTCAGACTTTTGTAATT10.4273504273504274No Hit
GGAAGGTGACGTGAAAGATCACTGTGCAGCAGCAATCTTGACTTCTGGAA10.4273504273504274No Hit
TCCTAGTCTTGCTAGTTCTTACTTTTTGTAGATTTTTTGTTCTACATTTA10.4273504273504274No Hit
CACTCCACCCCCAAGAATATCATCTAGTGCTGAACAGAAGGTGATTATGA10.4273504273504274No Hit
GGAGAGCAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCTATCA10.4273504273504274No Hit
GCTGAAGACCCCAAAGATCTCATGTTAAGTGGAGAAAGGGTTTTGCAAAC10.4273504273504274No Hit
GGTCTTTGCCTGCCGAGAGTTATTAACAGTGCAGTGTGAATCCAGAGTGA10.4273504273504274No Hit
GCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC10.4273504273504274No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG10.4273504273504274No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT10.4273504273504274No Hit
CACTCCACCCCCAAGAATATCATCTAGTGCGGAACAGAAGGTGATTATGA10.4273504273504274No Hit
TGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA10.4273504273504274No Hit
CAGAAGATCCAGAAGGCACTGAATTTAAACAATGGTCCTGTTGTTTGTTC10.4273504273504274No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACGTACT10.4273504273504274No Hit
GCCTCAAAGACAATGGCCCCTGGTTGTAGCTAACTAACTTCAGAACACCA10.4273504273504274No Hit
TGGGAAAAGACTAAAGGAACAGGAAGAAATCAGTAGCAAAAATCCTGCTA10.4273504273504274No Hit
TAGCAGAGAACGATGGGTGTTTCACCACAAGACTCTTTCCCAATATTTTG10.4273504273504274No Hit
TGAAAGAAAGTAACATTCAACACTGATACCCAAAATGAATGCACTCATTT10.4273504273504274No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC10.4273504273504274No Hit
CTCACCTTAAACTTCATTTTGGTTCTGTGTTTACAGATTAGAAAACGTCA10.4273504273504274No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC10.4273504273504274No Hit
AGTGAACTTGATGCTCAGTATTTGCAGAATACATTCAAGGTTTCAAAGCG10.4273504273504274No Hit
CTACTTTACCTTTCCAATTTGCTGAAGAGTGCAAACAATACATCATACTT10.4273504273504274No Hit
CCTTCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC10.4273504273504274No Hit
GCTGCCCCCACCATGAGGCCAGCATCCTGTGCCCTGGTAACTGCTGTGTA10.4273504273504274No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT10.4273504273504274No Hit
ACCTGGATATAGGTCTTTTGGATGGCTGCAAGTTCTTCACCAAGCGGAAC10.4273504273504274No Hit
TTTTTCAGTGGTTTAGGAGGGTTGCTTCCTTCAGCACTTCTTTTGAGCAC10.4273504273504274No Hit
CACTCAAGAGCACATGTACAGCTGTACCTGATCAGTTCTAGCTGAGCCAG10.4273504273504274No Hit
CTCGATAATCTGGATGATTCATTGTTGTTATGACAGTAAGATACAGTCTA10.4273504273504274No Hit
ATCTCCGCAAGAAAGGGCAGCCTCACCACGGGCTGCCCCCAGGGAGCACT10.4273504273504274No Hit
TGACAAGTTACACATCAAAACCCATCTTGATGAAGAAACTGGAGAAAAGA10.4273504273504274No Hit
GTGGATTAACTGACGAAGGATTTTGATTTCACGAATGGCTGTGATTGGGA10.4273504273504274No Hit
TCCTCTTCCGCACCCAACAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC10.4273504273504274No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG10.4273504273504274No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA10.4273504273504274No Hit
CCTCAGCTTGCCACCAGGAGTCGTTACCTTTGCTAAGCTCGGAGGGTTTC10.4273504273504274No Hit
CTGTCCTTTTCCACAATCACACATATTCTTTCAAACATACTCTGCAGGTG10.4273504273504274No Hit
GCAGGTGACCAACCACATAACTCGAGACAGGGACAGCGGGAGGCTCAAAC10.4273504273504274No Hit
CTTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC10.4273504273504274No Hit
AGAAACAGCTAATAAAAAGTTCTCCAAACTTACTGATTTTTTGTTTTCCT10.4273504273504274No Hit
CTGAATGTGACAGCAAACAGGTGCATTTATCACTTCGTAAATGCTCTGCA10.4273504273504274No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCATC10.4273504273504274No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGCAGTGGTAATCTACTGGGACG10.4273504273504274No Hit
CACGCACACACAAGCAGATTGCTCAGATTACTAGAGAGCTCCGGAGGACG10.4273504273504274No Hit
TCATTACACCAGTTCGTCCCTCAGAATAAGAATGTAGAACCATGTCGTCA10.4273504273504274No Hit
GGGAACTAGCGGTAGAAGTTGTTCTGTAGAACCATGTCGTCAGTGTAGAT10.4273504273504274No Hit
GAAAACTTTTATTGATTTATTTTTTGGGGGGAAATTTTTTAGGATCTGAT10.4273504273504274No Hit
TACTGGTGTGGGTTATAGAAGAAACATGAGAGATTTAGTTCAAAAGGAAG10.4273504273504274No Hit
GGATTCATTCATGCCATAGTGTGTTTATTTATACTTTAAGGTAACAAGTC10.4273504273504274No Hit
GCTGACCTAAAGCCGCCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT10.4273504273504274No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGGGAATTT10.4273504273504274No Hit
CCCCCTCCTCTGTTGCTGCACATCCGTGGGCGTGAGCGCTGCGAGATGTT10.4273504273504274No Hit
AAGTGAGGGCGGAACAGGTACCTTGCTAAGAATACGGATTATCTGCTTTA10.4273504273504274No Hit
TGCCCTGACTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTGC10.4273504273504274No Hit
TGAGAGGCGGAGGAGAATAAGATAAAAATCTCTCTACATTAGCTAGTACC10.4273504273504274No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTAGGCGTGAGCGCTTCGAGATGTT10.4273504273504274No Hit
CCAACTGGCAAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA10.4273504273504274No Hit
CTCGATAATCTGGATGACTCATTGTTGTTATGACAGTAAGATACAGTCTA10.4273504273504274No Hit
AAAAACCCACAGCAACTAGGTCTAATTACCCCCACTTGCCCCTTCTCGGT10.4273504273504274No Hit
CAGCCAGACTGCCTTCCGGGGTCACTGCCATGGAGGAGCCGCAGTCAGAT10.4273504273504274No Hit
CAGATGGGCTGGAAGTAAGGAAACATGTAATGATAGGCGGACTCCCAGCA10.4273504273504274No Hit
CCAACTGGCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCCC10.4273504273504274No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG10.4273504273504274No Hit
CAAAACACCTCAGAATTTACTTTTGCTAAATCCACTTAAGATATTAGTAT10.4273504273504274No Hit
ACAAGTCAACAACCCCCACAAAATGTTTAATTTAACTGACCTTAAAATTT10.4273504273504274No Hit
TAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT10.4273504273504274No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)