FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340615
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA234916.896642837220909No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC219486.44363871232917No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT175215.143930830996873No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC166764.895850153398999No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC163554.801608854571877No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA131603.863599665311275No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC120823.547113309748543No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA86072.5268998722898286No Hit
GTATAATGATGAGCATTTTGGCTACCGTACACATAGGTTAACATTTACCA79272.327260983808699No Hit
GGGGACGCTCTTGTATTATCTGTACACTGACTTATGGTATCAAATAATAA76612.249166948020492No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC73712.1640268338153046No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC72752.1358425201473805No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT72712.1346681737445503No Hit
GGGCAGTTGGCCACTTTTACTTACACTGACGACATGTTTCTTCTATAACC59341.7421428885985646No Hit
GATTCCCGCGCCCAGAGTCCCACGACCCCCTCAGACCAAGTCTCTGCTAC55991.6437913773615371No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAGCAC50871.493475037799275No Hit
GCCTACCAAAATCAGAGAAGCCATACACTGACACAATGGCAACAGTTCTG49951.4664650705341808No Hit
GGGATAATGGGGTTTTCCTGTGTACACTGACGACATTTGCTGCCATACAG46701.3710494253042291No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAGCACTGT44201.2976527751273432No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA43011.2627159696431456No Hit
CTCCTCCACAGCTCGCTACACTGACGACATTCAGAGTGAAGAAATTTTAC38971.1441069829572978No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCAAAAT35321.0369478736990445No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT34291.0067084538261672No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAGCA33960.9970200960028184No Hit
CTGTGGTGCCACCTAAGCTCTACACTGACGACATGGTTCTGATGAGCCAA33470.9826343525681488No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTAGCA29590.8687227514936219No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAG28950.849933209048339No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG28870.8475845162426786No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAGC28710.842887130631358No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG27360.8032529395358395No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG27090.7953261013167359No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTAGCACT26380.7744814526665004No Hit
CTGTGGTGCCACCTAAGCTCTGCACTGACGACATGGTTCTGATGAGCCAA25590.7512881112106043No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA23540.6911028580655578No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTAGCACTG22440.658808331987728No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTAGCAC22230.6526430133728697No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT20550.6033204644540023No Hit
GCCCTCTCAGAAACATCTGCTACCGTAACTTCGAGGGCTAAACCACAAGA19770.5804207095988139No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTAGC18220.5349147864891446No Hit
TGCATTCTGAAAAAGCCAGACTAAGTCTCTGCCGCTGTACCAATCTCAGA17950.526987948270041No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC17670.5187675234502297No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTAGCACTGTT17670.5187675234502297No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAGCACTG17630.5175931770473995No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTAGCACT17600.516712417245277No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG14890.43715044845353257No Hit
CTAACTACAATTCCCAGCAAGCCACACTGACGACATGGTTCTACTCACTC14300.4198288390117875No Hit
TGATAAATGCACCTGTTTGCTGTCACATTCAGAGACCAAGTCTCTGCTAC13930.4089661347856084No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAAA13730.4030944027714575No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCGTATTTTTCCAAATGTAATTT13570.39839701716013687No Hit
GCTTAGATTCCTCTTTGGAGTCAGTGTACCTCTCACAATATTAAATGCAC12650.3713870498950428No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTAGCACTGTTGCATC12300.36111151887027876No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT11630.34144121662287336No Hit
AATAGTGTAAAGCAGCATATAAAAATGACTCTAGGTAGACCAAGTCTCTG11120.3264682999867886No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT10500.3082659307429209No Hit
TTTTCCTGGCTTTGCTTACGACCTACGGTAGCAGAAACTACCTGCTGCCA10440.30650441113867566No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG10290.30210061212806244No Hit
TGGTGATAGATGACTTGCTGCTACACTGACCCATGTGGAATAGTTCCCAG9510.2792008572728741No Hit
AACAATCGACAGGCTAGAAGTTGAGACCAAGTCTCTGCTACCGTAGCACT9480.2783200974707514No Hit
ATTTAACACCTAGCCAAAAGGCAGACCAAGTCTCTGCTACCGTAGCACTG9220.2706868458523553No Hit
GACTACAGATGTGCCACCATACCCAGAGACCAAGTCTCTGCTACCGTAGC9050.26569587364032704No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCAAAACTAGTATCTT8720.2560075158169781No Hit
CACAAAGGTTTTTATAAGTTCTGTGGATCTAGACCAAGTCTCTGCTACCG8130.23868590637523304No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT7710.2263552691455162No Hit
ATCTCCTGGTTATTCTCAGTTTTCTTTACCAGTGCAAGACCAAGTCTCTG7480.2196027773292427No Hit
GAGTTTGATGCACAACTTCAAGGCAATCTCAAGACCAAGTCTCTGCTACC7350.21578615152004463No Hit
GAGTTTGATGCACAACTTCAAGGCACACTGACTTATGGTATCAAATAATA7250.2128502855129692No Hit
TCAGATATAAAAGAAGAGGTCTTGGCCAGTTGGAGACCAAGTCTCTGCTA6960.2043362740924504No Hit
AGGCTGGGTTTGCACTGACACAATGGCAACAGTTCTGAGACCAAGTCTCT6930.20345551429032777No Hit
GGGAAAAGAACAGGCTTCACCTAAAAACGTAGACCAAGTCTCTGCTACCG6010.17644554702523377No Hit
CACCATTTATCTGAGGACTGGAGACCAAGTCTCTGCTACCGTAGCACTGT5860.1720417480146206No Hit
ACTCTACCCTGCGATTTTCTCTCTTCGTCATACCTGTTCCGCCCTCACTT5720.16793153560471502No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA5670.16646360260117726No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAGAGACCAAGTC4610.13534342292617765No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACGAGA4550.13358190332193237No Hit
AGCAATCTGCTTGTGTGTGCGTGAGACCAAGTCTCTGCTACCGTAGCACT4080.11978333308867783No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC4010.11772822688372502No Hit
TCCAAAGTAATATCCAATGTAAAAGATGCAGACCAAGTCTCTGCTACCGT4000.11743464028301748No Hit
ATCCTGTTCCAAGTCAAGACCAAGTCTCTGCTACCGTAGCACTGTTGCAT3960.1162602938801873No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAATACGGAC3930.11537953407806467No Hit
AAGTCCTCCATTTCTGTATCCATGGAGGAAGTAGGCAGCTGAGACCAAGT3630.10657193605683836No Hit
AACTGAGCGACCTCAGAGACCAAGTCTCTGCTACCGTAGCACTGTTGCAT3410.1001130308412724No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)