FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340615
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT230836.776859504132231No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG218286.4084083202442645No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC173165.083745577851826No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA165764.866491493328245No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA162514.771075848098293No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT130293.8251398206185874No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG115893.402375115599724No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT85362.506055223639593No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTACAGATAATACAAG75322.211294276529219No Hit
AGGGGTGGTAAATGTTAACCTATGTGTACGGTAGCCAAAATGCTCATCAT74972.201018745504455No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG73282.15140260998488No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT72222.1202824303098806No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG71582.1014928878645978No Hit
TACTGGTGTGGGTTATAGAAGAAACATGTCGTCAGTGTAAGTAAAAGTGG56311.6531861485841786No Hit
GAGGGGGTCGTGGGACTCTGGGCGCGGGAATCTGTAGAACCATGTCGTCA55541.6305799803296976No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT50881.4937686243999824No Hit
CAGAACTGTTGCCATTGTGTCAGTGTATGGCTTCTCTGATTTTGGTAGGC49711.4594189921171998No Hit
GTCTCTGTATGGCAGCAAATGTCGTCAGTGTACACAGGAAAACCCCATTA46431.3631225870851253No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC43981.291193869911777No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT42931.2603672768374852No Hit
GTTGTAAAATTTCTTCACTCTGAATGTCGTCAGTGTAGCGAGCTGTGGAG38471.1294276529219205No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTGACTTGGTCTGTCTAAGAACACA34801.0216813704622523No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT34000.9981944424056486No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA33930.9961393362006958No Hit
CACAGAGACTTGGCTCATCAGAACCATGTCGTCAGTGTAGAGCTTAGGTG33190.9744139277483376No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA29650.8704842710978671No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA28890.8481716894440938No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC28660.8414191976278202No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG28380.833198772808009No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT27020.7932709951117831No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC26600.7809403578820663No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT25820.7580406030268778No Hit
CACAGAGACTTGGCTCATCAGAACCATGTCGTCAGTGCAGAGCTTAGGTG25400.7457099657971611No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT23320.6846439528499919No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT22420.6582211587863129No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT22200.651762253570747No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT20680.6071370902632004No Hit
TGTGGTTTAGCCCTCGAAGTTACGGTAGCAGATGTTTCTGAGAGGGCTGT19530.5733746311818329No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG18020.5290430544749938No Hit
GAGATTGGTACAGCGGCAGAGACTTAGTCTGGCTTTTTCAGAATGCATGT17730.520529043054475No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG17670.5187675234502297No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT17590.5164188306445694No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG17540.5149508976410316No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC17270.507024059421928No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG14850.4359761020507024No Hit
GCAGATGTCTTTGAGTGAGTAGAACCATGTCGTCAGTGTGGCTTGCTGGG14210.4171865596054196No Hit
CTGAATGTGACAGCAAACAGGTGCATTTATCATGTAGAACCATGTCGTCA13730.4030944027714575No Hit
TGTACCATGTACTAAAAAATTACATTTGGAAAAATACGGTAGCAGATACA12910.3790203015134389No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT12350.36257945187381646No Hit
TGAAGTGCATTTAATATTGTGAGAGGTACACTGACTCCAAAGAGGAATCT11830.3473129486370242No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG11400.33468872480659984No Hit
ACCTAGAGTCATTTTTATATGCTGCTTTACACTATTTGTAGAACCATGTC11000.3229452607782981No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT10430.3062108245379681No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT10430.3062108245379681No Hit
GGTGGCAGCAGGTAGTTTCTGCTACCGTAGGTCGTAAGCAAAGCCAGGAA10400.30533006473584545No Hit
GGGAACTATTCCACATGGGTCAGTGTAGCAGCAAGTCATCTATCACCATG9540.2800816170749967No Hit
CAACTTCTAGCCTGTCGATTGTTTGTAGAACCATGTCGTCAGTGTAGATC9500.27890727067216653No Hit
GCCTTTTGGCTAGGTGTTAAATTGTAGAACCATGTCGTCAGTGTAGATCT9170.26921891284881755No Hit
CTGGGTATGGTGGCACATCTGTAGTCTGTAGAACCATGTCGTCAGTGTAG8800.2583562086226385No Hit
GGTGAAATAAAGGAAGATACTAGTTTTGCAGAGACTTGGTCTAGTGAGGA8490.2492550240007046No Hit
AGATCCACAGAACTTATAAAAACCTTTGTGTGTAGAACCATGTCGTCAGT8110.23809873317381797No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC7540.22136429693348797No Hit
TGCACTGGTAAAGAAAACTGAGAATAACCAGGAGATTGTAGAACCATGTC7460.21901560412782758No Hit
TGAGATTGCCTTGAAGTTGTGCATCAAACTCTGTAGAACCATGTCGTCAG7380.21666691132216725No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTGCCTTGAAGTTGTG7290.21402463191579935No Hit
CCAACTGGCCAAGACCTCTTCTTTTATATCTGATGTAGAACCATGTCGTC6930.20345551429032777No Hit
CAGAACTGTTGCCATTGTGTCAGTGCAAACCCAGCCTTGTAGAACCATGT6860.20140040808537496No Hit
ACGTTTTTAGGTGAAGCCTGTTCTTTTCCCTGTAGAACCATGTCGTCAGT5950.17468402742098849No Hit
CCAGTCCTCAGATAAATGGTGTGTAGAACCATGTCGTCAGTGTAGATCTC5860.1720417480146206No Hit
AAGTGAGGGCGGAACAGGTATGACGAAGAGAGAAAATCGCAGGGTAGAGT5700.16734436240329992No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT5650.1658764293997622No Hit
CTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA4550.13358190332193237No Hit
CGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT4520.13270114351980974No Hit
CACGCACACACAAGCAGATTGCTTGTAGAACCATGTCGTCAGTGTAGATC4120.12095767949150801No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG4040.11860898668584764No Hit
TGACTTGGAACAGGATTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG4020.11802181348443257No Hit
CCAGATCCTTAACATTGGTCCGTATTGTAGAACCATGTCGTCAGTGTACT3960.1162602938801873No Hit
GCATCTTTTACATTGGATATTACTTTGGATGTAGAACCATGTCGTCAGTG3900.11449877427594204No Hit
CAGCTGCCTACTTCCTCCATGGATACAGAAATGGAGGACTTTGTAGAACC3590.1053975896540082No Hit
CTGAGGTCGCTCAGTTTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG3450.10128737724410257No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)