FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303137
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC142514.701174716382362No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT98743.2572731141365128No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC98233.240449037893758No Hit
CCGGTACAAACCTTTCATTGTATTGTCGTTTTAGTTCAACCAGACCAAGT87942.9009985584075846No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA78222.58035145825155No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG75892.5034885216915126No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT69432.29038355594995No Hit
TTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTA61712.035713225373346No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA55781.840092103570333No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC55641.8354737296997727No Hit
TGTTTCAGGAAGGAATGTTCCCAATAGTAGACATAAAAGTCTTCGCACAG49491.632595163243022No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA48071.5857516568416261No Hit
ATATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTG48021.58410223760214No Hit
TTTTTGTCCAGCCAGCAAATGAGAGTCTGTACCCGACCATTTCACAAAAG47861.5788240960357858No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT46561.5359391958091555No Hit
CTCCCAAAATTCAAAATCCTCATACGGTAGCAGCATCTTGAATCTCATAC46181.5234036095890637No Hit
CCTATCTCATCGTCTTATATTGGCTTCACTGTCTTTCAACAGGCCTTGCT43841.4462107891811293No Hit
CAGCAGTTACCAGGGCACAGGGTCAGTGCAGTTTCCAGGGGACCAGGACC42501.4020063535629104No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA41481.3683582010774007No Hit
GCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATTGAACTT38541.271372349795637No Hit
ATTACAACCCATCTGAGTGGAGCCCTGGCTTCTCAGGCAGACCTGGTGTC37671.2426724550285844No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA34291.131171714439346No Hit
AACTGAGCGACCTCAGAGCCCTGGCAGCTCCAAGGCGCCTTTGCCAGCAG34001.1216050828503283No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA33761.1136878705007967No Hit
TTTTGTGTTTGCACAGTGCCTTTCAGAATGTCCCACCCATAGAGTAGCAG33011.08894658190851No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTACGT32801.08201902110267No Hit
ATTTCTCTTCTGACCTCTCAATTTTACCTTTTCTAGATGTATTCTTGGGG32471.0711328541220637No Hit
GGTCCAGCAGCCCTTTCCTGAGCAAGCGGTCTCTGAGTCGGAGTCCACTC30971.0216502769374902No Hit
AGAGGAACGTTACACACCAGCCATAGATGTTTGGAGCTGTGGGTAAGGTT29780.9823940990377289No Hit
ATTTACCCTACCAATTGCCCAGTGCGGGACTACCAGCTGCACATTTCCCG29560.9751366543839913No Hit
AATTTGCATCTGTTTGTCCACATTAGGCTTAGGTTACCACAAGGATCTCC27670.912788607131429No Hit
CTAATGAGCGGACGGCAAAGAACGCTTTTTCAGACGTGGGGCTCAAGTAT26390.8705634746005931No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT26350.8692439392090046No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTACG25520.8418635798335405No Hit
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA25400.8379049736587747No Hit
GGAGTTGAACCCAGCCGTGACAGCCGCCTTGCTGCAACTTTTATCCCAGC24640.812833801218591No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTACGTATCT24560.8101947304354138No Hit
TTTGCTAATAGATGCCTAAGCCCAGAAAGGGTGCTTCTTCAACTAAAATA24340.8029372857816762No Hit
GGATACAGTAGCCAGAATGACGACTCAATCAGTGCTTCGGTTGTTGCTTT24070.7940304218884531No Hit
GGAAATTGAAGAAATCTGCCTAGTAGATGAGAACCAGTTCACCTTAACCA23990.7913913511052759No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG22550.7438880770080855No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC21700.7158479499368272No Hit
CTGTGGTGCCACCTAAGCTCTTAAGTGATAATTTATGTAGACAAACTGAA21500.7092502729788841No Hit
CCAAGGAACATCTTCAGTATCTCTAGGATTCTCTGAGCATGGCAGTTTCT20190.6660354889043567No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT19330.6376654779852015No Hit
TTAACTACAATTCCCAGCAAGCCGCGGAGCCAACCTGGGCCTCTGCCGGC18030.5947805777585713No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC17710.5842242946258622No Hit
GCAACAGCTTCATTGCTGAGGGACTAGACATCACTCGCCTGTAACTTGAA16230.5354014851370832No Hit
GAAGAGACCAAGCAAGTTTTGAGACTCAGTCTGACAGAGTTCTCATTACC15710.5182475250464311No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC15640.5159383381111511No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC15570.5136291511758709No Hit
GCCCTCTCAGAAACATCTGCAGGAGGACTTGGGGAAACAAATTCATATGA15490.5109900803926938No Hit
ACAATAGTGGCCAAAAATGCTCATTGATAATAACAGTTTACATTTGTTTT14200.4684350640139607No Hit
ATGAGCAAGTTGGGGTGTGCTACACTGACGACATGGTGGTTGATCTTAAG13930.4595282001207375No Hit
CTCACGTAATTTTGTACTTTTTCTTCCTATTGGTCTTTGTTTTTCTCTAG13810.4555695939459716No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC13610.44897191698802846No Hit
CTACTCTTTAGCTTCCTACCTAAGAATAAAAATGGGATTGTTTGCACTAA13300.4387455177032167No Hit
TTTTGTGCGAAGGAAACCGATGGGGATCGGAACCGTAGCGGTTGAGCTGC13150.4337972599847593No Hit
CCAGTGTTGTGTTTCCCAAAGTCTTTCCTGTTGTGTAAGTATCTCCTTTT12770.42126167376466744No Hit
TATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTGA12730.41994213837307887No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA12630.41664329989410726No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACGCGATATATGT12420.4097157390882671No Hit
AGCACCAAACGTAAAGCAGCAACAGAGGAGGGGGAGACCAAGTCTCTGCT12420.4097157390882671No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTACGTATC12250.4041077136740154No Hit
GGCAGTTCTCTTGAGTCAGCTGATGAAAACCCAAGAGCCAGCAGGCAGTC11490.37903654123383157No Hit
CTGAGGGAAGCAGCAATTCTGTGGTACATGCAGAGACCAAATTGCAAAAC11380.37540781890696284No Hit
CCAGACTCTCCAGAACCAAAGGCAATCACACCACCTCAGCAACCATATAA10840.3575940911205165No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC10540.3476975756836018No Hit
GCCTACCAGTCCAGCACCCGGTCACCGAGCCCCTACAGTAGGCGACAGAG10430.3440688533567331No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTACGTA10330.34077001487776154No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCGATATATGT10260.33846082794248145No Hit
CCACTGTAAGTTTATGTCATGGTTGTTTTTTATATTTCAGTCGCCCTTAC10180.33582175715930423No Hit
GGGATCGATGAAGGACCGGATATCGGGAAGTTCAAAGCGTTCGAATGAGG10130.33417233791981843No Hit
TCTCCACTTCACCCCGTCACCACCACTTTCCAGGTTGGTTCTACTGCTGT9900.32658500941818386No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA9640.31800802937285777No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCAATATATGT9280.3061322108485602No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAC9250.3051425593048687No Hit
TTCAACGTTGCCTCCTTTGCCCCTCCCACCCTTATTACCTGGAGATGATG8880.292936856932674No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTACGT8640.2850196445831423No Hit
TCGGGGGCCTACAAGAGTCCCACCAAGAGGGGGAAGAGGGAGAGGAGTTC8600.28370010919155364No Hit
CACCTGTCGGGAAGATTTGGCCAGACACGCCATGCGCCGGCCGCCTGATG8060.2658863814051073No Hit
TCCCCTTTGCTTTGTCTTTTTCCAGCCTCCCCCACTGGCAGGATTGCCAT7530.24840253746655802No Hit
CGACACATGACTGCAATGAAATTCAGTCCTGGAAGGAAAAGAAGAAGTAA7480.24675311822707227No Hit
GGGATAAAAGCACAAAGGTCTGCTACCGTAACCTTCAGAGCTAGGGAAAA7200.2375163704859519No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG7160.23619683509436326No Hit
CAACTTGTATTCAGCAGGTACCGCACTTCTTGAACAAAACCTGCATACTT6880.2269600873532429No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCGGATATATGT6820.22498078426586No Hit
TATTTCTGGACAGTCTACGAAAAGCTCTTGCTGGCCATGGAGGAAGTAGG6760.22300148117847707No Hit
TGATAAATGCACCTGTTTGCTGTACACTGACGACATGTTTCTTCTATAAC6500.21442450113315104No Hit
AGATGATACTGCTGCCCTCGGACTGCCGAGGACACAAAGCCAGGCCTAAA6240.205847521087825No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACTCCTGATGGCAAGCTGAGG6230.20551763723992783No Hit
CCTCAGGGCCTCAGAGAAAGGCAAAAGGGCCTAGACTTTGACTTCCTTTT5980.19727054104249894No Hit
GAAGAGACCAAGCAAGTTTTGAGAACACTGACGACTTGGTCATTCTCTCC5900.19463147025932168No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT5710.18836367714927574No Hit
AGGTTCAACTACGCGTGTAAATTCTACTGCAAATGCAGAGACACAAGCAA5660.18671425790978996No Hit
TCCTCTTGAGATGGGTAGTTTCTATTCTGAAGACTCCCAGAGCAACTGTG5540.1827556517350241No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTACGTAT5460.18011658095184685No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCAAA5310.17516832323338952No Hit
TCGGGGGCCTACAAGAGTCTGACAGAGTTCTCATTACCCAAGACCAAGTC5160.1702200655149322No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTACGTA4960.16362238855698907No Hit
TGAAGAAGGAGATGGTGTGGAATTGATGGAAGCCAAATCACAGTTATTTC4920.16230285316540047No Hit
GACAAAAATCATCTCTCCGAAAAACAAGTCTCTGCTACCGTACATCTTCT4890.16131320162170898No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG4580.15108680233689717No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT4510.14877761540161707No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACACTCGGTAGCA4450.14679831231423415No Hit
TATGTCATGATTCATCTTACTAGCCTCAAACATATCTTCTTTGCCAGGAC4340.14316958998736545No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCCAACTCCCTGGCTTTCAGACCA4340.14316958998736545No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG4150.13690179687731951No Hit
CTTTCTAGTTGATACGGCCTTCACTATGTAAAGGTCAGTCTTTTTATTTC4100.13525237763783374No Hit
GCAGGAAGGAAAAGGAACCTGTATTCTTGTAGGTGGTCATGAAATCACTT4020.13261330685465647No Hit
CTCAGTCACACATGCCAGAGATTGCTCAGGAAGCAATGGAGGTAAGGGGA3970.13096388761517072No Hit
AGTCTTCAGAATGCCAGAGATATACAGGATATGCGAATTAAGAAGAAACA3930.1296443522235821No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTACGTATCTCGAATC3910.12898458452778777No Hit
TCAAATGTAGCAAATCAGAAGCCCTTTGAGAGTGGAAGTGACAAAATCTC3720.12271679141774182No Hit
TTCAGGTGTATTTTGGAGGCCATGGCAGGCAGATCACCTGAGGTCAGGAG3700.1220570237219475No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTACGTATC3670.12106737217825604No Hit
GTGTTTGTTCCAATACAGCAGATGAAATATTACCTAGATCTTGCCTTGGC3630.11974783678666741No Hit
AGAAGACCAGGAAAGAACGGGAGCTGAAGTCTGGGCACAAAGACCGGAGT3610.1190880690908731No Hit
GGTGCCAGTCTTGCTCACAGGAGAGAATATTGTGTCCTCCCTCTCTGACA3570.11776853369928449No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG3570.11776853369928449No Hit
GCTATGCATCTACCACCCTCACCCCTAAATCCTCCTGACTGCTGGCCTCA3500.11545934676400439No Hit
CCCAAGATTACTGGCATCTTCCTGGGGCTGGCTCACCTGTCGGGAAGATT3440.11348004367662146No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC3370.11117085674134136No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC3320.10952143750185558No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTACGTAT3320.10952143750185558No Hit
GGTATCAGATGCTTCATTACAAAACGCAAGACAAGTGTTTTCTGAAATAG3240.10688236671867835No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG3230.1065524828707812No Hit
CTGACACTTACAACAGTCAAGTTCTGATAGAAGCTACAGTAATAGCACTA3160.1042432959355011No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)