FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303137
Sequences flagged as poor quality0
Sequence length150
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG141934.682041453204326No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC98003.232861709392123No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA97613.219996239324134No Hit
GGTTGAACTAAAACGACAATACAATGAAAGGTTTGTACCGGTGTAGAACC87652.891431926818567No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT77192.5463734219181426No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT75592.4935920062545978No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT67642.231334347176359No Hit
GGTTGCTGAGGTGGTGTGATTGCCTTTGGTTCTGGAGAGTCTGGGGGAGA61942.0433005538749804No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG54391.7942382487126283No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG53831.7757647532303875No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT48951.614781435456576No Hit
AGCTTCATCATACCTTTCACTAAGACAAGAATTTAGAAGTGGACAGGAAA47281.5596908328577508No Hit
GGAAGGTGACGTGAAAGATCACTGTGCAGCAGCAATCTTGACTTCTGGAA46841.5451759435502759No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT46211.524393261132755No Hit
GTATGAGATTCAAGATGCTGCTACCGTATGAGGATTTTGAATTTTGGGAG45621.504930114106823No Hit
CAGCTGTGCCATTTGAGGGATGCTCAGGTCGGTTTGGCTCTGCAGCAGGT42911.415531591326694No Hit
TTATTTTTTGGGGGGAAATTTTTTAGGATCTGATTCTTCTGAAGATACCG42251.3937592573654816No Hit
TGCAATTTGGTCTCTGCATGTACCACAGAATTGCTGCTTCCCTCAGCCTT41281.3617605241194577No Hit
CAACTGTCAGTCTGCCATTCTTTAAGACAGCTAAGAGGGGAGGATCTAAC38211.2604861828150309No Hit
AGGGCCTATTAGCCATATCTCCCAGCTCTCAACAGGAGGTGGAGAGGCAG36391.2004473224977485No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC34081.1242441536335057No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT33941.1196257797629454No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT33571.1074200773907508No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA33081.09125576884379No Hit
AAAGAGAGTGAGTCGTTGCGAAGCCCTGTGTTTCAGCTCATTGTGATTAA31941.0536490101835143No Hit
CAATGATGAACACTGCGGGCTTCCAGACAATGTTAACACGCTGAATGCCA30381.0021871299115581No Hit
TTATCGCCATCCTTCTGAACTTAAGAAGAGCTCTAAAAAGCCAGTGTACA29020.957322926597545No Hit
CTGTGTCACCAAGGGTTTCGTTGGGGCCATGAAGACCACCTTTCCTGAAG28150.9286230318304926No Hit
CACTGGGCAATTGGTAGGGTAAATCCACAGGGCGCCCGCGGAGGTGCAGA27660.9124587232835318No Hit
TCCACCAATCTCTCAAACCGATCAGCCAATACTGTTTCTGCAAGTGTGTC26520.8748519646232562No Hit
CGACAAGCAACACATCATCGTCCGACTCCAGCTGAGCCTCCGCTGCTGCT25910.8547290499015296No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA25660.8464819537041007No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG25460.8398842767461577No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACAATATCACC24690.8144832204580766No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC24590.8111843819791051No Hit
GTAGTCAGTGCATTCTACAACAGCGCGCATGTTAGCAAGTTCATCAACCA24390.8045867050211621No Hit
GTGTCAATGGCACTGAACCCTGTTTCAGGCCCATCAGTGTTTCCATAAGT24270.8006280988463961No Hit
GTCACTGACAACTGGCTTGTGCAACATTTTGACATGGAAGTCACAGACTA23660.7805051841246696No Hit
AGGAAATAATGAGCGAACCCCACTTCCCCACAACGGCACCCCACATGAAA23120.7626914563382233No Hit
CTGTGACAGTTCCCAGCGGGTCCGGATATGAATGATAGACTGGACAATGG23010.7590627340113546No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG22710.74916621857444No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG21700.7158479499368272No Hit
ACAAGTTGCAGGACTTTTTGCTGTTTCTTTTTCTACATTTTCATGTACTT20530.67725153973286No Hit
CAGATGGGCTGGAAGTAAGGAAACATGTAATGATAGGCGGACTCCCAGCA19570.6455826903347331No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT19260.6353562910499213No Hit
CACTCAAGAGCACATGTACAGCTGTACCTGATCAGTTCTAGCTGAGCCAG17280.5700392891662845No Hit
ACTTTCAAGTTTCCCCACCCTTTACACAGTTTCCCCGCGGAAAACGCCGC17210.5677301022310044No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG16430.5419991620950263No Hit
TGCCTGAATGTTCTTCACCACCTTCATGCAAACGTAAAGTTGGTGGTACA15890.5241854343085799No Hit
CACTCAAGAGCACATGTACAGCTATACCTGATCAGTTCTAGCTGAGCCAG15670.5169279896548425No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA15530.5123096157842824No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG15480.5106601965447966No Hit
TAGGAGCCAGGAGAGAATGACCTGTCCCGGTAACTGGAACGGAAGGCAAT15410.5083510096095165No Hit
TGTGGTTTAGCCCTCGAAGTAAGAAAGTCAGATATGTTGTGAGTAAAGCT14890.49119704951886445No Hit
GTGGATTAACTGACGAAGGATTTTGATTTCACGAATGGCTGTGATTGGGA14140.46645576092657776No Hit
CACAACCTTGCACTGCTTTATGAAGTGTTTCACCAAACATATTTCGAAGT14100.4651362255349891No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG13600.4486420331401314No Hit
ATGTTACCTTAAGATCAACCACCATGTCGTCAGTGTAGCACACCCCAACT13540.44666273005274837No Hit
AACATGTTCCAGAGCAGTTGGTAGAGTAAATGCCGAGAATCAAACAGAGT13020.4295087699620963No Hit
CTGAGGTCGCTCAGTTCCGGAGCTGCAACCCGGAGTCCCAGATGATCGGG12820.4229110930041532No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT12660.41763295143779877No Hit
ATCACAATATCTCATTTTGCCCTCTTTGCAACCAGTGCACATTACTGGGT12610.415983532198313No Hit
GAAAACTTTTATTGATTTATTTTTTGGGGGGAAATTTTTTAGGATCTGAT12560.41433411295882716No Hit
CCCCCTCCTCTGTTGCTGCTTTACGTTTGGTGCTTGTAGAACCATGTCGT12420.4097157390882671No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT12370.4080663198487812No Hit
GCACCTGAGCTCAATGAGATTCATGACCGGTCTGTTTACCTGCTGCCTCC11380.37540781890696284No Hit
TACTGGTGTGGGTTATAGAAGAAACATGAGAGATTTAGTTCAAAAGGAAG10880.35891362651210507No Hit
TCCCTCTTCCCCCTCTTGGTGGGACTCTTGTAGGCCCCCGATAGAGTTTT10860.3582538588163108No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG10490.346048156444116No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT10270.3387907117903786No Hit
TTACCCACAGCTCCAAACATCTATGGCTGGTGTGTAACGTTCCTCTCCTA10090.33285280252822985No Hit
CGACTCAGAGACCGCTTGCTCAGGAAAGGGCTGCTGGACCGCCTTCGACC9970.32889419635346395No Hit
GCAATGGTTAAGGTGAACTGGTTCTCATCTACTAGGCAGATTTCTTCAAT9960.3285643125055668No Hit
GGTCTTTGTGCCCAGACTTCAGCTCCCGTTCTTTCCTGGTCTTCTCCTTG9940.3279045448097725No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT9580.31602872628547485No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA9320.3074517462401488No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA8700.2869989476705252No Hit
CATGAGGGTAGTGTTGACAAAGATTAAAACAGAAAATGCTTTATGTTTAG8660.28567941227893656No Hit
AATGCAGATGATTACCTCGCTCTGGCAAATGATTTCATTAAAGATTCAGA8330.2747932452983305No Hit
AGCATTATGGATCTGTAAGTCTGTTTCTGCTGCAGGTGACCAACCACATA7560.24939218901024948No Hit
GGATCCGTGGCGAGTTCGTCCACTGTTACAGTGGCCAGTGGTAGGGGAGA7470.2464232343791751No Hit
CAGTACTTGTCCCTGAGGTAGTTTCTTTTCGAGAAGTTTTGGATGGAGGT7340.24213474435651208No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT7050.23256811276749456No Hit
AATAGCAACAAGAAAAGATGGAAGAGTACTAAATTTGAGTAATCTAGGAA6880.2269600873532429No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTATACAGGCATCAGGAGAGC6820.22498078426586No Hit
ACTTTTTCCCTAGCTCTGAAGGTTACGGTAGCAGACCTTTGTGCTTTTAT6820.22498078426586No Hit
TACTGGTGTGGGTTATAGAAGAAACATGTCGTCAGTGTACAGCAAACAGG6420.21178543034997377No Hit
ATGTTACCTTAAGATCAACCACCATGGACTGAACAAGTAGGAAAATGACA6400.21112566265417948No Hit
CCTCAGCTTGCCATCAGGAGTACGGTAGCAGAGACCTGTGGGAAGCGAAA6170.2035383341525449No Hit
GACGGCTGCTCTTTTCGTTCTGTCGCCTGCCCGATGGACGAGCCTCCCGG5980.19727054104249894No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACAGTATCACC5870.19364181871563022No Hit
TAGGAGCCAGGAGAGAATGACCAAGTCGTCAGTGTTCTCAAAACTTGCTT5840.19265216717193875No Hit
CTGGGTATGGTGGCACATCTGTAGTCCTAGCTGCTCAGGAGGCTGAGGTG5830.1923222833240416No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG5670.1870441417576871No Hit
CACTGGGCAATTGGTAGGGTAAATCCACAGGGCGCCCGCGGAGGCGCAGA5540.1827556517350241No Hit
GCCCAGCAAGTATGATTTGTCCTTTCACAATTGGTGGCGATGGTTTTCTC5530.18242576788712694No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC5510.18176600019133263No Hit
GTCCATGCTCTTGAAAATAATCATCTACTAACCTTTTTTATAAAACAGTG5450.1797866971039497No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTGAGACTTGGTCTGTCTAAGAACA5270.1738487878418009No Hit
TGGGTAATGAGAACTCTGTCAGACTCTTGTAGGCCCCCGATGTAGAACCA5090.16791087857965212No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT4980.1642821562527834No Hit
TTTGAAACAGAAGCAGAAGATGTACGGTAGCAGAGACTTGTTTTTCGGAG4880.16098331777381183No Hit
CCTGTCATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTA4550.1500971507932057No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG4510.14877761540161707No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT4450.14679831231423415No Hit
AGTGAGGATGAAGAGCTTCCCTGCTTCCAACACTTGTTATTTGGTAAAGT4350.1434994738352626No Hit
GAAAGCCAGGGAGTTGGGTACGGTAGCAGTAGGGGGGCTTTCTCTGTAGA4330.14283970613946828No Hit
TCTAAAGTATGCAGGTTTTGTTCAAGAAGTGCGGTACCTGCTGAATACAA4160.13723168072521666No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT4090.13492249378993657No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT4050.13360295839834796No Hit
TCAGATTGAGACCTCCTTTCCACCACCATGCGATTACTCACGATGTAATT4030.13294319070255364No Hit
TGCAAGGACCGTACAGTGCCTCAGTGATGCCAGCCTCCGAGACATGCCAC3840.12667539759250768No Hit
CAGAAATGACATCTACCTGTTACTCACAGAAACCACACACCAAAGGAACA3760.12403632680933042No Hit
TTCTTTGTAAGGCGTGTCTCAAACTCCTGACCTCAGGTGATCTGCCTGCC3690.12172713987405036No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTATTTTCTCCATCTGGGCTC3680.12139725602615319No Hit
TATGAGAACACGCAGAGGGAACTTGGCCTCCTACTGCTGCTTTCAGAGAG3630.11974783678666741No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT3620.11941795293877026No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACCTCCATGATCTCCAACAAAG3500.11545934676400439No Hit
CTTGGCCATAAGAAGCTCCCGAGGGGCTATCATCTTGTTTGGAGCTGTCA3500.11545934676400439No Hit
GTAGAGGGCCTGGGTTAAGTATGCAGATTACTGCAGTGATTTTACATCTA3450.1138099275245186No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACGATATCACC3450.1138099275245186No Hit
ACATGCACAGTTGCTCTGGGAGTCTTCAGAATAGAAACTACCCATCTCAA3420.11282027598082714No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG3410.11249039213293No Hit
GTACCTTTTCCCCTGTCTTCCTCCTAACCTCTTGTGTTGATTTTCTTCTC3300.1088616698060613No Hit
GCAGGTGACCAACCACATAACTCGAGACAGGGACAGCGGGAGGCTCAAAC3260.10754213441447266No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC3260.10754213441447266No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCCC3220.10622259902288406No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT3210.10589271517498688No Hit
TGAAAGAAAGTAACATTCAACACTGATACCCAAAATGAATGCACTCATTT3120.1029237605439125No Hit
GTTCTGGAGTACGTATAGCAGTATTTTCTTCTCTTGTGAGCTGGTCTGAA3090.10193410900022103No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)