FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297642
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC174075.848300979028497No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC128454.315587181916531No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT118753.989692314928673No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG97243.267012048030856No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA93893.1544607279886576No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA77362.5990955577505863No Hit
CCTATCTCATCGTCTTATATTGGCTTCACTGTCTTTCAACAGGCCTTGCT69362.3303162860080233No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC59451.997365963136923No Hit
CCAAATAACAAGTGTTGGAAGCAGGGAAGCTCTTCATCCTCACTAGATAA51931.7447134476989135No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT46501.5622795170036488No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCCTGAAAACCAGAT45601.5320418489326102No Hit
GGAAACACTGATGGGCCTGAAAGTCACACCACCTCAGCAACCAGACCAAG44461.493740802709295No Hit
TTAACTACAATTCCCAGCAAGCCGCGGAGCCAACCTGGGCCTCTGCCGGC43131.449056248782094No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA43111.4483843006027375No Hit
GGTCCAGCAGCCCTTTCCTGAGCAAGCGGTCTCTGAGTCGGAGTCCACTC39581.3297854469463315No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTATAT38991.3099629756553175No Hit
ACGCCTTCTGTCTGAGCACCCAGAATTAGAACATATCATCTGGACCCTTT38881.3062672606688572No Hit
GCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATTGAACTT37101.246463872706137No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTATATGCGC29490.9907875904610236No Hit
CTAACAGTACTCGGCCTGCTCGCTGGTATACCAAACTTGGATTCTTTCCT29390.9874278495642417No Hit
TATGTCATGATTCATCTTACTAGCCTCAAACATATCTTCTTTGCCAGGAC28200.9474469328925353No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG27880.9366957620228328No Hit
GGCAGTTCTCTTGAGTCAGCTGATGAAAACCCAAGAGCCAGCAGGCAGTC27740.9319921247673381No Hit
CCAGTGCAACAGGTGGCTTGGGATCAATAAAAGCTGAGGTGATGGCAGAT27150.912169653476324No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC26290.8832758817639983No Hit
GTATAATGATGAGCATTTTGGCTACCGTACACATAGGTTAACATTTACCA26260.8822679594949638No Hit
TTCTGATGAGCCAAGTCTCTGTGGGAGGAGGGCAGTACACAGACCAAGTC24390.8194408047251396No Hit
GCCTACCAGTCCAGCACCCGGTCACCGAGCCCCTACAGTAGGCGACAGAG24360.8184328824561049No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT24090.8093615820347936No Hit
TTTTGTGCGAAGGAAACCGATGGGGATCGGAACCGTAGCGGTTGAGCTGC24050.8080176856760806No Hit
TTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTA24010.8066737893173679No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTATA23800.7996183334341255No Hit
CAGCAGTTACCAGGGCACAGGGTCAGTGCAGTTTCCAGGGGACCAGGACC23500.7895391107437795No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT23460.7881952143850667No Hit
CGATGCGCGGGAAGACGTTCCGCTTTGAAATGCAGCGGGATTTGGTGAGT22720.7633331317488795No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG21710.7293997486913809No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC21700.7290637746017027No Hit
ACCTCCATCCAAAACTTCTCGAAAAGAAACTACCTCAGGGACAAGTACTG21340.7169687073732874No Hit
GCAGAACATTTTGTTTCCTCACTACACTGACGACATGTTTCTTCTATAAC21180.7115931219384362No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC20930.703193769696481No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC18010.6050893355104454No Hit
CAGCATCCTGCCCTTACCTGTTCCTCCTCATCCTGGGTTTTAGCCTGAAG17730.5956820609994558No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTATATG17630.5923223201026737No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTATATGCG16730.5620846520316353No Hit
GGATACAGTAGCCAGAATGACGACTCAATCAGTGCTTCGGTTGTTGCTTT16540.5557011443277494No Hit
AAACGTGATGAGACCCGAGTTATGATCTTCTCTTCATTTCGAGATAGTGT16190.5439420511890123No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA15830.531846983960597No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTTTCTCCTGGCTCCTATGAG15680.5268073726154239No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA15620.5247915280773546No Hit
AGAGGAACGTTACACACCAGCCATAGATGTTTGGAGCTGTGGGTAAGGTT15380.5167281499250778No Hit
CCAAGGAACATCTTCAGTATCTCTAGGATTCTCTGAGCATGGCAGTTTCT14580.48985022275082146No Hit
AATTTGCATCTGTTTGTCCACATTAGGCTTAGGTTACCACAAGGATCTCC14580.48985022275082146No Hit
TTAAATTTCACTCCACTTACCTACCAAGGGCCTGGTTTAAGGCCCTGTAT14390.48346671504693556No Hit
CTACTCTTTAGCTTCCTACCTAAGAATAAAAATGGGATTGTTTGCACTAA14090.47338749235658945No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA13780.46297229557656516No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC13290.4465095651823331No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT13250.44516566882362035No Hit
AGTTCCATTTAGGTTGCTGGTGGTCGGTAGCAGACAGGTATTGCACATGG13100.44012605747844724No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG13040.43811021294037805No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCGATATATGT12780.42937488660874473No Hit
CCACTGTAAGTTTATGTCATGGTTGTTTTTTATATTTCAGTCGCCCTTAC12640.42467124935324985No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC12440.41795176755968577No Hit
GGCCAGGGGTTGTGCTTTTTAAATTTCAATTTTATTTTTGCTAAGTATTT11560.3883860476680039No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTATAT11540.38771409948864743No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAT11220.37696292861894487No Hit
GTTGTATCCGCTGCTTTGTCCTCAGAGTTCTCACAGTTCCAAGGTTAGAG11210.3766269545292667No Hit
TTTTTGTCCAGCCAGCAAATGAGAGTCTGTACCCGACCATTTCACAAAAG10830.36385993912149495No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACATTCCTGCCATCA10600.35613253505889625No Hit
TCTAAGCATAGCATTCAATTTTGGCCACTACAAATCATTCTCCTTAAGAC10560.35478863870018346No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA10420.35008500144468857No Hit
GTCCTCCAGAGCACAAACCTGTGGCAGATACACACTGGTCCAGCCTTAAC10420.35008500144468857No Hit
CTTCTTCAGGTCAGGGGAAAGGGAATGCCCAATTCAGAGAGACATGGGGG10390.349077079175654No Hit
CCAGTGTTGTGTTTCCCAAAGTCTTTCCTGTTGTGTAAGTATCTCCTTTT10240.3440374678304809No Hit
TTGTGGCACCAAATACGAAACACCCATAAAGAAAAAAGAACTGAATTCTC9820.32992655606399635No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT9680.32522291880850146No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCAATATATGT9640.32387902244978867No Hit
GAAGTATTACAAATGGAAGATGATCTGGTGCACTTTTCTGAGGACCATAA9350.3141357738491207No Hit
GTTAGAAGGCTGGCTCCCATGCTGTTCTAACACAGCTTCTAGTTCAGCCA9280.3117839552213733No Hit
CTCACGTAATTTTGTACTTTTTCTTCCTATTGGTCTTTGTTTTTCTCTAG9210.3094321365936259No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG9150.30741629205555665No Hit
GCTTTCAAGTGATAATTGCCTTCATTTTAGGCTTGGGAAGATACACATGC9130.3067443438762003No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA9020.30304862888974No Hit
TTTGCTAATAGATGCCTAAGCCCAGAAAGGGTGCTTCTTCAACTAAAATA8750.2939773284684285No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG8700.2922974580200375No Hit
GAAGAGACCAAGCAAGTTTTGAGACTCAGTCTGACAGAGTTCTCATTACC8590.2886017430335773No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG8110.2724749867290235No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT7480.2513086190792966No Hit
TCGGGGGCCTACAAGAGTCCCACCAAGAGGGGGAAGAGGGAGAGGAGTTC7430.2496287486309056No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTATATGC7370.24761290409283637No Hit
TCTGGGGTATCAGGTAGGTGTCTACGGTAGCAGAAAGTCAGTCCCATGGA7260.24391718910637614No Hit
CGAAGGGAGAACGACGAACGTCGTGGATCAGATCGGAGCGACCGCCTGCA7250.2435812150166979No Hit
CTGTGGTGCCACCTAAGCTCTTAAGTGATAATTTATGTAGACAAACTGAA7150.24022147411991587No Hit
TTTTGTGTTTGCACAGTGCCTTTCAGAATGTCCCACCCATAGAGTAGCAG6940.2331660182366736No Hit
CTGTATGTAGTCGGTGCTGTGACTTGTTTGTGCTCATCTCTGTTCTGTAG6780.22779043280182232No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTATATGCG6730.2261105623534313No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTATATG6290.21132770240759033No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT6190.2079679615108083No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA6040.2029283501656352No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA5650.18982536066818526No Hit
TCAATGGCAAGTAAGTTATTTATGGTCGTTTTTAAGGATAAGCTGTTAAC5540.18612964568172502No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG5500.18478574932301223No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG5100.17134678573588405No Hit
CGGCAGTTGTGTCTAGAGAATGGCGGGTTCTGCACCTCCGCGGGCGCCCT5010.1683230189287802No Hit
TTTTCCTGGCTTTGCTTACGACTATGGTAGCAGAAACTACCTGCTGCCAC4970.1669791225700674No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTATATGCGCTGCATC4900.16462730394231997No Hit
TTTTGTGTTTGCACAGTGCCTTTATTATGAAGGTAGGAGACAGAAGCAGG4840.16261145940425073No Hit
AAAGGGTCCCACTGCTACTAACTAGCCTCCTCTTTGTCAGGCCAAGCACA4840.16261145940425073No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG4820.16193951122489433No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC4790.1609315889558597No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC4770.16025964077650331No Hit
GCAGGAAGGAAAAGGAACCTGTATTCTTGTAGGTGGTCATGAAATCACTT4620.15522002943133026No Hit
AGAAGACCAGGAAAGAACGGGAGCTGAAGTCTGGGCACAAAGACCGGAGT4610.15488405534165206No Hit
CGACACATGACTGCAATGAAATTCAGTCCTGGAAGGAAAAGAAGAAGTAA4520.15186028853454822No Hit
CCAATTTTGGTAAGCTGCCCATCTACATTATCAGGCAAATGGCTGCAAAG4490.1508523662655136No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCTGACATATGT4350.14614872901001874No Hit
CCCAAGATTACTGGCATCTTCCTGGGGCTGGCTCACCTGTCGGGAAGATT4310.14480483265130595No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTATATGC4150.13942924721645467No Hit
TATCAACTGACCCAGGCAAATCCTTTGAATGGCATGCAGACCAAGTCTCT4150.13942924721645467No Hit
AGATGATACTGCTGCCCTCGGACTGCCGAGGACACAAAGCCAGGCCTAAA4130.13875729903709827No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCTCCCGACATATGT3830.12867807634675213No Hit
CGGCCCCTTCCGCTTTACGTCTGACGTCACGCCGCACGCCCCAGCGAGGG3750.12599028362932652No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCGGATATATGT3660.12296651682222268No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT3480.116918983208015No Hit
TCAAAGGGCTCCACTGGTTTTTCTGAGCAGGACTTCACTTTTTCAAGCTT3370.11322326822155475No Hit
TTCCTGAGTTTTCATGGACAGCACTTGAGTGTCATTCTTGGGATATTCAA3230.10851963096605989No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACACTCGGTAGCA3210.1078476827867035No Hit
AGTTAAAGAAATGTGTAGTGCTAAATGTGATACGGTAGCAGATACACACT3090.10381599371056503No Hit
GTAACTTGATTTGCTGTTGTATTTGCTACCGTAACCCTCAATTTGGAAGC3040.10213612326217401No Hit
GAAGTTCATCCTGTTTTAAGTCACACTTGTGATTTGTTAAATTTTTTAAC2990.100456252813783No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)