FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297642
Sequences flagged as poor quality0
Sequence length150
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG173115.8160474664193895No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA127474.282661721128067No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC117473.9466876314498625No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT95953.2236713904623673No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT93073.1269108526350444No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG78382.633364914897763No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG75742.5446677552227173No Hit
CAGCTGTGCCATTTGAGGGATGCTCAGGTCGGTTTGGCTCTGCAGCAGGT69052.319901089227999No Hit
AGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGG52161.7524408517615122No Hit
GCTGTCTACCTGACCAATCGATGGGGCATTCATTATGACATGAAGATCAG45311.5222986003319423No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA45121.5159150926280567No Hit
GGTTGCTGAGGTGGTGTGACTTTCAGGCCCATCAGTGTTTCCTGTAGAAC44071.4806378132118452No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT42481.4272179329530108No Hit
ACTTTCAAGTTTCCCCACCCTTTACACAGTTTCCCCGCGGAAAACGCCGC41841.4057155912136057No Hit
CAATGATGAACACTGCGGGCTTCCAGACAATGTTAACACGCTGAATGCCA38831.304587390220466No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA38621.297531934337224No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTGCTTGATTTTCTTACTTGGTCCAA38221.2840929707500959No Hit
CAACTGTCAGTCTGCCATTCTTTAAGACAGCTAAGAGGGGAGGATCTAAC36551.2279852977738357No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACCTCCATGATCTCCAACAAAG34691.1654941170936897No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC29180.9803723936809993No Hit
ACCTGTATAGGGTATGCTCTTTGAATAATTACATCAACACAACCAACATT28700.9642456373764455No Hit
GCACCTGAGCTCAATGAGATTCATGACCGGTCTGTTTACCTGCTGCCTCC27460.9225848502563482No Hit
TCTAAAGTATGCAGGTTTTGTTCAAGAAGTGCGGTACCTGCTGAATACAA27370.9195610834492444No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG26990.9067940680414727No Hit
AACAGATATGCTATAGTACAGAAGGCAAAAGAAAAGTGATTACCTTGCTT26320.884283804033033No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG25940.8715167886252613No Hit
AGGGGTGGTAAATGTTAACCTATGTGTACGGTAGCCAAAATGCTCATCAT25670.8624454882039497No Hit
GGTTGCTGAGGTGGTGTGATTGCCTTTGGTTCTGGAGAGTCTGGGGGAGA24230.8140652192902883No Hit
GTGTACTGCCCTCCTCCCACAGAGACTTGGCTCATCAGAATGTAGAACCA24080.8090256079451152No Hit
CTGAGGTCGCTCAGTTCCGGAGCTGCAACCCGGAGTCCCAGATGATCGGG23900.8029780743309076No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT23760.7982744370754127No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG23620.793570799819918No Hit
CGACTCAGAGACCGCTTGCTCAGGAAAGGGCTGCTGGACCGCCTTCGACC23540.7908830071024923No Hit
TGCAATTTGGTCTCTGCATGTACCACAGAATTGCTGCTTCCCTCAGCCTT22720.7633331317488795No Hit
CCTCAGCTTGCCATCAGGAGTCGTTACCTTTGCTAAGCTCGGAGGGTTTC22520.7566136499553154No Hit
GCACAAAGGCCCCATTCTGGCACTGACCTATTGCATCCCCAGCCCCAGAC21460.7210003964494258No Hit
TACTGGTGTGGGTTATAGAAGAAACATGTCGTCAGTGTAGTGAGGAAACA20730.6964742879029169No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG20490.68841090975064No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA18040.60609725777948No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG17740.596018035089134No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT17550.589634527385248No Hit
CCATCTGGACCCTCTCTGAAGTGTCAAGTTCATCCAATGCCCCCACCCCC17520.5886266051162135No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT16590.5573810147761404No Hit
AATTACCTCCAGTTGCTCCTTCTGGGTAAAACCCTTCGTGCTTTTCCCTG15990.5372225693954482No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT15710.5278152948844584No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT15640.5254634762567111No Hit
AGGAAATAATGAGCGAACCCCACTTCCCCACAACGGCACCCCACATGAAA15530.5217677612702508No Hit
TTATCGCCATCCTTCTGAACTTAAGAAGAGCTCTAAAAAGCCAGTGTACA15080.5066489272347316No Hit
CAGATGGGCTGGAAGTAAGGAAACATGTAATGATAGGCGGACTCCCAGCA14210.4774191814327279No Hit
TCCACCAATCTCTCAAACCGATCAGCCAATACTGTTTCTGCAAGTGTGTC13760.4623003473972087No Hit
AACATGTTCCAGAGCAGTTGGTAGAGTAAATGCCGAGAATCAAACAGAGT13620.4575967101417139No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT13590.4565887878726793No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG13290.4465095651823331No Hit
CAAATATACCATGTGCAATACCTGTCTGCTACCGACCACCAGCAACCTAA13000.4367663165816652No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG12640.42467124935324985No Hit
TTACCCACAGCTCCAAACATCTATGGCTGGTGTGTAACGTTCCTCTCCTA12450.418287741649364No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT12420.4172798193803294No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACAATATCACC12110.4068646226003051No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCCC12070.40552072624159224No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA11490.3860342290402564No Hit
CGCGTTGCCTTTGTTTCTTCTTAATTCGCATATCCTGTATATCTCTGGCA11450.38469033268154357No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA11150.3746111099911975No Hit
ACATGTTAGCTGACTGATGATGGTCAATTTATTTTGTCCATGGTGTCAAG10770.36184409458342576No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTATCTCG10670.3584843536866437No Hit
GGAAGGTGACGTGAAAGATCACTGTGCAGCAGCAATCTTGACTTCTGGAA10560.35478863870018346No Hit
TAAGGAGAATGATTTGTAGTGGCCAAAATTGAATGCTATGCTTAGATGTA10460.3514288978034014No Hit
GTCACCAACCCCATGTCTTTGGAGTGTTTGGACTTATGCCGCTTGTGCTT10380.3487411050859758No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT10310.34638928645822836No Hit
CGTCAGCTATTGAATTACTTTCCAAAAGAGAAATTTCATTGAATTTTTTA10090.33899785648530784No Hit
TGGTGGCAAACTCTCCTTCTCAACCAAAAAGAAAGCAATCAACTATTTCA10010.3363100637678822No Hit
ATCACAATATCTCATTTTGCCCTCTTTGCAACCAGTGCACATTACTGGGT9870.33160642651238736No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT9590.32219915200139765No Hit
TATGGTCCTCAGAAAAGTGCACCAGATCATCTTCCATTTGTAATACTTCT9260.3111120070420169No Hit
CACAACCTTGCACTGCTTTATGAAGTGTTTCACCAAACATATTTCGAAGT9120.30640836978652203No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTCTTGGTGCCATTTATCGTTTTTG9050.3040565511587746No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT8950.30069681026199263No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT8940.3003608361723144No Hit
AAGTACATATATTTAGCAGATCAGTTAACAGACAAAAGTCAACTTTACAG8920.29968888799295795No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT8780.2949852507374631No Hit
GTCACTGACAACTGGCTTGTGCAACATTTTGACATGGAAGTCACAGACTA8590.2886017430335773No Hit
TAGGAGCCAGGAGAGAATGACCTGTCCCGGTAACTGGAACGGAAGGCAAT8380.281546287150335No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATATCTACAATATCACC8300.27885849443290933No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG8160.2741548571774145No Hit
GGTTGAACTAAAACGACAATACAATGACCACCATTCAAAATTGAGAGGTC7540.2533244636173658No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG7480.2513086190792966No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC7260.24391718910637614No Hit
CCATGGGACTGACTTTCTGCTACCGTAGACACCTACCTGATACCCCAGAT7210.2422373186579851No Hit
AATGCAGATGATTACCTCGCTCTGGCAAATGATTTCATTAAAGATTCAGA7190.2415653704786287No Hit
GAACGCTTTGAACTTCCCGATATCCGGTCCTTCATCGATCCCGACTTGCT7090.23820562958184666No Hit
AAAGAGAGTGAGTCGTTGCGAAGCCCTGTGTTTCAGCTCATTGTGATTAA6770.22745445871214412No Hit
ACAAGTTGCAGGACTTTTTGCTGTTTCTTTTTCTACATTTTCATGTACTT6620.22241484736697106No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT6600.22174289918761464No Hit
AGAAACAGCTAATAAAAAGTTCTCCAAACTTACTGATTTTTTGTTTTCCT6470.217375236021798No Hit
GGTTGAACTAAAACGACAATACAATGACCACCATTCAAAATTGAGAAGTC6330.21267159876630315No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT6210.2086399096901647No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT6170.20729601333145187No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC5920.19889666108949677No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT5610.18848146430947244No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGACGTCTACAATATCAAC5480.1841138011436558No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT5300.17806626752944812No Hit
CATTCCTTGTTGTGCTCAGTACTGACTTTGGTATGTGTGTTCTGCTGTCC5130.17235470800491867No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT5050.16966691528749303No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT5010.1683230189287802No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC4920.16529925212167637No Hit
GGTGGCAGCAGGTAGTTTCTGCTACCATAGTCGTAAGCAAAGCCAGGAAA4920.16529925212167637No Hit
ACCAGCGGTAGAAATTGTACATGACCACGGCGGCAATAAAGGTCTTTCCC4880.16395535576296355No Hit
TCCCTGCTTCTGTCTCCTACCTTCATAATAAAGGCACTGTGCAAACACAA4790.1609315889558597No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG4790.1609315889558597No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG4750.15958769259714692No Hit
CTTGGCCATAAGAAGCTCCCGAGGGGCTATCATCTTGTTTGGAGCTGTCA4550.15286821080358282No Hit
GGATCCGTGGCGAGTTCGTCCACTGTTACAGTGGCCAGTGGTAGGGGAGA4530.15219626262422642No Hit
TCAGATTGAGACCTCCTTTCCACCACCATGCGATTACTCACGATGTAATC4510.15152431444487No Hit
TATTAAAAGGTTACTCCTCACATCACCCCATTTTTCCTTATATTTGGCTT4370.14682067718937514No Hit
GCCGGTTGTAAAGAGCCATGTATCATAACTGCTTGCGAAGATGTAGTTTC4290.14413288447194952No Hit
GCAGGTGACCAACCACATAACTCGAGACAGGGACAGCGGGAGGCTCAAAC4260.14312496220291493No Hit
GCATGCCATTCAAAGGATTTGCCTGGGTCAGTTGATATGTAGAACCATGT4170.1401011953958111No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC4120.13842132494742007No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACATTATCACC4060.13640548040935083No Hit
GACGGCTGCTCTTTTCGTTCTGTCGCCTGCCCGATGGACGAGCCTCCCGG3850.12935002452610855No Hit
TCCTCAGCAGTCTGGAACCCCGCAGACACCAGCTTCACCCGCACCGCTGG3680.12363846500157909No Hit
TCCTTGGATGATGATGCTTTCACGGCTCCATTTCATAGGGATGGAATGCT3380.11355924231123296No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT3340.11221534595252014No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA3320.11154339777316373No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACAGTATCACC3200.10751170869702528No Hit
CACTTGCTGAGTGTGTTTCTCAAACAATTTAATTTCAGGAGCCTACAAGA3040.10213612326217401No Hit
AAGAGGCTTCCAAATTGAGGGTTACGGTAGCAAATACAACAGCAAATCAA3040.10213612326217401No Hit
CTGGACCAGTGTGTATCTGCTACCGTATCACATTTAGCACTACACATTTC3020.1014641750828176No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)