FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31696
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC775024.45103483089349No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC579918.29568399798082No Hit
TGCTTGGATAGTTGAAATGACGACACTGACGACATGGTGGTTGATCTTAA29559.322942958101969No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA24397.694977284199898No Hit
TCTACCCACTCTCTTTTCAGTGCTACGGTAGCAGAACTGTCAAATGACCA17115.398157496214033No Hit
GATTCCCGCGCCCAGAGTCCCACGACCCCCTCAGACCAAGTCTCTGCTAC16335.152069661786976No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA5071.5995709237758708No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT3921.2367491166077738No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA2750.8676173649671883No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA2260.7130237253912166No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTACCT2150.678319030792529No Hit
TCTAAGCATAGCATTCAATTTTGGCCACTACAAATCATTCTCCTTAAGAC2150.678319030792529No Hit
GAAGTATTACAAATGGAAGATGATCTGGTGCACTTTTCTGAGGACCATAA2150.678319030792529No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATTCCCAGAAGAGCCCAAT2070.6530792529025745No Hit
TGACAGTTTGACAGTTAAAGGCATTACGGTAGCAGCATCTTGAATCTCAT1620.51110550227158No Hit
TGTTCATTCATGATCCCACTGCACTCCAGCCTGGATAACAGAGGAAGACA1610.5079505300353356No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC1540.48586572438162545No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTACC1410.44485108531044926No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTG1310.41330136294800607No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTACCTCGTT1290.4069914184755174No Hit
TCTACCCACTCTCTTTTCAGTGCTACGGTAGCAGAACTGTCAAATGACCG1000.3154972236244321No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTACCT760.23977788995456842No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA740.23346794548207975No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC690.21769308430085815No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAAACCAAGTCTC660.20822816759212517No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC640.20191822311963653No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG590.18614336193841494No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAC490.15459363957597172No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG490.15459363957597172No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC480.1514386673397274No Hit
AGCAATCTGCTTGTGTGTGCACACTGACGACATGGTTCTACAGGGAAGCT460.14512872286723877No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTACCTC460.14512872286723877No Hit
ATACGGCCAGGCATTGAAGTCACACTGACGACAGGGGCCAGGAGGAGACC400.12619888944977284No Hit
CCATTTTCTTCACACCTGTCGTCAGTGTACCAGGTTAGTGTGTAAATCCA400.12619888944977284No Hit
TGCAATTCAGTACAATTAGGTGGGTACGGTAGCAAAGGCCAATCAAGTGA400.12619888944977284No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACGAGA380.11988894497728421No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT370.11673397274103987No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTACCTC360.11357900050479555No Hit
GCATAATCGAGGGAGAGTTTGCTACCGTAATTGGCGCTGGAATCCGAATT320.10095911155981827No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAAAC320.10095911155981827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)