FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31696
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG778924.574078748107016No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA580118.301993942453308No Hit
ATGTTACCTTAAGATCAACCACCATGTCGTCAGTGTCGTCATTTCAACTA27208.581524482584554No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT24297.663427561837456No Hit
TGGTCATTTGACAGTTCTGCTACCGTAGCACTGAAAAGAGAGTGGGTAGA16785.294043412417971No Hit
GAGGGGGTCGTGGGACTCTGGGCGCGGGAATCTGTAGAACCATGTCGTCA16465.193084300858152No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT5041.5901060070671376No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC3831.208354366481575No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT2710.854997476022211No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT2230.7035588086824837No Hit
TATGGTCCTCAGAAAAGTGCACCAGATCATCTTCCATTTGTAATACTTCT2150.678319030792529No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA2110.6656991418475517No Hit
TAAGGAGAATGATTTGTAGTGGCCAAAATTGAATGCTATGCTTAGATGTA2110.6656991418475517No Hit
CGAATTGGGCTCTTCTGGGAATTACGGTAGCAGCATCTTGTTCTGTTTGT2060.6499242806663301No Hit
CTGTTACCTTAAGATCAACCACCATGTCGTCAGTGTCGTCATTTCAACTA1630.5142604745078243No Hit
GTACCTTTTCCCCTGTCTTCCTCTGTTATCCAGGCTGGAGTGCAGTGGGA1610.5079505300353356No Hit
GTATGAGATTCAAGATGCTGCTACCGTAATGCCTTTAACTGTCAAACTGT1600.5047955577990914No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG1460.4606259464916709No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG1410.44485108531044926No Hit
CAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT1300.4101463907117618No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC1240.39121655729429583No Hit
CGGTCATTTGACAGTTCTGCTACCGTAGCACTGAAAAGAGAGTGGGTAGA990.3123422513881878No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT760.23977788995456842No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA730.23031297324583544No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG680.21453811206461385No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTGCACACA660.20822816759212517No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG630.1987632508833922No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT590.18614336193841494No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA510.1609035840484604No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG490.15459363957597172No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT480.1514386673397274No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG450.14197375063099443No Hit
CCTCCTGGCCCCTGTCGTCAGTGTGACTTCAATGCCTGGCCGTATTGTAG410.12935386168601715No Hit
TGGATTTACACACTAACCTGGTACACTGACGACAGGTGTGAAGAAAATGG390.12304391721352853No Hit
TTCTTCACTTGATTGGCCTTTGCTACCGTACCCACCTAATTGTACTGAAT380.11988894497728421No Hit
CGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT380.11988894497728421No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC370.11673397274103987No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT370.11673397274103987No Hit
AATTCGGATTCCAGCGCCAATTACGGTAGCAAACTCTCCCTCGATTATGC320.10095911155981827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)