FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35858
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC884124.65558592224887No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC624117.404763232751407No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT412811.51207540855597No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA17694.933348206815773No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC14644.082770929778571No Hit
CCTCAGGGCCTCAGAGAAAGGGTGGGGAAACTTGAAAGTAGACCAAGTCT14103.932176920073624No Hit
GGTCCAGCAGCCCTTTCCCTTGCTCTGGCCCGCAGTGTTCATCATTGAGA13913.8791901388811425No Hit
GAAAGTGGTGCATTGATGGAAGGGAGCTTCTTATGGCCAAGAGACCAAGT5311.4808410954319817No Hit
TCCAAATACTCCACACGCAAAGACATGGGGTTGGTGACAGACCAAGTCTC4651.296781750237046No Hit
AGGCTGGGTTTGCACTGACGACATGGTTCTACAAGCTGGTATTGCTCCCA3090.8617323888671985No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT3030.8449997211222042No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGGAGACCAAGTCT2580.7195047130347482No Hit
GGCTTCCACTTAATTCCAGTCTGGGTGCGGAAGAGAAAGACCAAGTCTCT2140.5967984829047911No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTAGTCCCACGACCCC2030.5661219253723019No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTACAG1990.5549668135423058No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTACA1920.5354453678398127No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA1750.48803614256232913No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC1590.4434156952423448No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACGAGA1260.351386022644877No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTACAGGTCA1050.2928216855373975No Hit
CCTCAGGGCCTCAGAGAAAGGGTGGGGAAACTTGAAAGGAGACCAAGTCT1040.29003290757989847No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAGACCAAGTCTC950.2649339059624073No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA850.23704612638741704No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA660.18405934519493558No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTACAGG600.16732667744994142No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC590.1645378994924424No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC590.1645378994924424No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAC520.14501645378994923No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTACAG480.13386134195995317No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA480.13386134195995317No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG450.1254950080874561No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAAAC450.1254950080874561No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTACAGGTC390.10876234034246192No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)