FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35858
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG883624.641642032461377No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA625017.4298622343689No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT412811.51207540855597No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT17644.919404317028278No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG14584.066038262033577No Hit
ACTTTCAAGTTTCCCCACCCTTTCTCTGAGGCCCTGAGGTGTAGAACCAT14113.9349656980311223No Hit
CAATGATGAACACTGCGGGCCAGAGCAAGGGAAAGGGCTGCTGGACCTGT13933.8847676947961403No Hit
CTTGGCCATAAGAAGCTCCCTTCCATCAATGCACCACTTTCTGTAGAACC5321.4836298733894808No Hit
GTCACCAACCCCATGTCTTTGCGTGTGGAGTATTTGGATGTAGAACCATG4691.3079368620670422No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC2960.8254782754197112No Hit
AGCTCCATGTGGGAGCAATACCAGCTTGTAGAACCATGTCGTCAGTGCAA2960.8254782754197112No Hit
CCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT2560.7139271571197502No Hit
TTCTCTTCCGCACCCAGACTGGAATTAAGTGGAAGCCTGTAGAACCATGT2110.5884321490322941No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA2040.5689107033298009No Hit
GAGGGGGTCGTGGGACTAGACTTGGTCTAAGACCCAGGGCAGCTACGGTT1980.5521780355848067No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG1900.5298678119248146No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT1800.5019800323498244No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG1610.4489932511573429No Hit
CGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT1260.351386022644877No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC1070.29839924145239555No Hit
CCTTTCAAGTTTCCCCACCCTTTCTCTGAGGCCCTGAGGTGTAGAACCAT1030.2872441296223994No Hit
CCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCATG960.26772268391990633No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT850.23704612638741704No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT700.19521445702493168No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT600.16732667744994142No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG590.1645378994924424No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG580.1617491215349434No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA520.14501645378994923No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA490.13665011991745218No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA480.13386134195995317No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT470.13107256400245412No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT390.10876234034246192No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)