FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310610
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT196466.3249734393612576No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA161245.191075625382313No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC156315.032355687196163No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG153864.953478638807508No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA131714.240365731946814No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA110063.5433501819001316No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT102603.3031776182350856No Hit
CCAAATAACAAGTGTTGGAAGCAGGGAAGCTCTTCATCCTCACTAGATAA101673.2732365345610246No Hit
GCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATTGAACTT87392.813496023952867No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC82222.6470493544959917No Hit
CCTATCTCATCGTCTTATATTGGCTTCACTGTCTTTCAACAGGCCTTGCT67562.1750748527091854No Hit
ATATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTG60981.9632336370367987No Hit
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA59511.9159074080036058No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC43151.3892018930491614No Hit
GGAAATTGAAGAAATCTGCCTAGTAGATGAGAACCAGTTCACCTTAACCA42671.3737484305077108No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG41181.3257783072019576No Hit
TTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTA38571.2417501046328192No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA37331.2018286597340717No Hit
GGTCTCTCATGGCACTGTACTCTTCTTGTCCAGCTGTATCCAGTATGTCC34791.120054087118895No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT33181.0682205981777793No Hit
TGTTTCAGGAAGGAATGTTCCCAATAGTAGACATAAAAGTCTTCGCACAG32061.0321625189143944No Hit
GGTCCAGCAGCCCTTTCCTGAGCAAGCGGTCTCTGAGTCGGAGTCCACTC31191.004153118058015No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCCTGAAAACCAGAT31151.002865329512894No Hit
GTTAGAAGGCTGGCTCCCCGGGGTGATGTGAGGAGTAACCTTTTAATAAG29870.9616560960690255No Hit
TGAACTTATATATTTGACACAACCCAGCAACCCATTCAGCAGAATTAGAC29820.9600463603876244No Hit
ACCTCCATCCAAAACTTCTCGAAAAGAAACTACCTCAGGGACAAGTACTG28040.9027397701297447No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACACTCGGTAGCA26290.8463990212807058No Hit
CTCAGTCACACATGCCAGAGATTGCTCAGGAAGCAATGGAGGTAAGGGGA25850.8322333472843758No Hit
CGACACATGACTGCAATGAAATTCAGTCCTGGAAGGAAAAGAAGAAGTAA24500.7887704838865459No Hit
GAAGAGACCAAGCAAGTTTTGAGACTCAGTCTGACAGAGTTCTCATTACC23850.7678439200283314No Hit
CAGCAGTTACCAGGGCACAGGGTCAGTGCAGTTTCCAGGGGACCAGGACC22140.7127909597244133No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA21160.6812401403689514No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC20470.6590257879656161No Hit
CCAGTGCAACAGGTGGCTTGGGATCAATAAAAGCTGAGGTGATGGCAGAT17940.5775731624867197No Hit
CGATGCGCGGGAAGACGCTGTAGAACCATACCCTCAATTTGGAAGCCTCT17640.567914748398313No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG17440.5614758056727086No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA15100.4861401757831364No Hit
TTAAATTTCACTCCACTTACCTACCAAGGGCCTGGTTTAAGGCCCTGTAT14840.47776955023985057No Hit
ACGCCTTCTGTCTGAGCACCCAGAATTAGAACATATCATCTGGACCCTTT14390.46328192910724053No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT13360.4301213740703777No Hit
ATTCATGGGTTGCAAGTAGAAGTCTGTGTGTGCTGAGGTATGTCAAAGAC13360.4301213740703777No Hit
TCTGGGGTATCAGGTAGGTGTCCAGCTCCTGGCACTGGTAGAGTGCTACA13330.429155532661537No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTACTG12710.40919481021216314No Hit
TTTTTGTCCAGCCAGCAAATGAGAGTCTGTACCCGACCATTTCACAAAAG12160.3914877177167509No Hit
TTACCCAGTACCATCAATGCTGTTAACAGTTCTTCATCCTTTTTCCAGCT11040.35542963845336595No Hit
TATGTCATGATTCATCTTACTAGCCTCAAACATATCTTCTTTGCCAGGAC10770.3467370657737999No Hit
GGGATCGATGAAGGACCGGATATCGGGAAGTTCAAAGCGTTCGAATGAGG10280.33096165609606903No Hit
TTTTGTGTTTGCACAGTGCCTTTCAGAATGTCCCACCCATAGAGTAGCAG10170.3274202375969866No Hit
GACCTTTTCCTCTGCCCTTATCATCGCTTTTCAGTGATGGAGGAAATGTT9450.3042400437848105No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC9450.3042400437848105No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA9050.29136215833360163No Hit
TGACACACTTGCAGAAACAGTATTGGCTGATCGGTTTGAGAGATTGGTGG8970.2887865812433599No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC8920.28717684556195877No Hit
TTGTGGCACCAAATACGAAACACCCATAAAGAAAAAAGAACTGAATTCTC8530.27462090724703003No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTACTGGAG8470.27268922442934873No Hit
ATTTCTCTTCTGACCTCTCAATTTTACCTTTTCTAGATGTATTCTTGGGG8330.2681819645214256No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT8070.2598113389781398No Hit
CTCACGTAATTTTGTACTTTTTCTTCCTATTGGTCTTTGTTTTTCTCTAG8060.25948939184185954No Hit
GTCCTCCAGAGCACAAACCTGTGGCAGATACACACTGGTCCAGCCTTAAC7840.2524065548436947No Hit
TCCCCTTTGCTTTGTCTTTTTCCAGCCTCCCCCACTGGCAGGATTGCCAT7770.25015292488973306No Hit
TCCCTTAGAGCTTCCACACATGGACTGGTCATTAATATCATTCACTCTCT7770.25015292488973306No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTACT7710.24822124207205176No Hit
AATTTGCATCTGTTTGTCCACATTAGGCTTAGGTTACCACAAGGATCTCC7620.24532371784552975No Hit
TGTGATTATTTCTATTTTAGCAACCAAAGGACACAATGAGATTAGATGAA7600.24467982357296933No Hit
GGAAACACTGATGGGCCTGAAAGTCACACCACCTCAGCAACCAGACCAAG7390.23791893371108463No Hit
TTAACTACAATTCCCAGCAAGCCGCGGAGCCAACCTGGGCCTCTGCCGGC7170.23083609671291971No Hit
AAAAGCAGTGGTCCAATGTATCTGCTCTACATCTATTGTGATGGCCACGT7080.22793857248639776No Hit
CCTCTCTCCTTCTTCCCCAAGTACACACACAAACCTGCCAATGCCAGTGG7050.22697273107755706No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTACTGG6980.22471910112359553No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC6820.21956794694311194No Hit
CTAACAGTACTCGGCCTGCTCGCTGGTATACCAAACTTGGATTCTTTCCT6800.2189240526705515No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC6780.21828015839799106No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG6740.21699236985287018No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTACTGGAGG6440.20733395576446348No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAC6170.19864138308489745No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA5950.19155854608673256No Hit
ATCTGGTGTTGAAAATTCTAATGACTTTGCATTTTTGAAGGTTTTTCCTT5920.19059270467789188No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT5760.18544155049740832No Hit
GTAACTTGATTTGCTGTTGTATTTGCTTAGGAAGAGCAGCACTTCAGAAA5710.18383181481600722No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG5570.17932455490808408No Hit
ACAATAGTGGCCAAAAATGCTCATTGATAATAACAGTTTACATTTGTTTT5520.17771481922668297No Hit
GCCAAACGAAAATTATGGCAGGTTGTTACGAGGCATTGGATGATTCAGAG5490.1767489778178423No Hit
TTCAACGTTGCCTCCTTTGCCCCTCCCACCCTTATTACCTGGAGATGATG5490.1767489778178423No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTACTG5410.17417340072760054No Hit
CTACTCTTTAGCTTCCTACCTAAGAATAAAAATGGGATTGTTTGCACTAA5280.16998808795595763No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC5030.16193940954895208No Hit
CCAGTGTTGTGTTTCCCAAAGTCTTTCCTGTTGTGTAAGTATCTCCTTTT4990.16065162100383118No Hit
AAGGGAAAAGGATGGATTAGGGTACACTGACGACATGGTTCTACTACCCT4610.14841762982518272No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG4260.13714948005537492No Hit
CTAATGAGCGGACGGCAAAGAACGCTTTTTCAGACGTGGGGCTCAAGTAT3880.12491548887672643No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT3800.12233991178648466No Hit
GCTGCCCCAAAGTGTAAAGAAATGCAGAATTCTCTCAATAATGATAAAAA3710.11944238755996264No Hit
ATTACAACCCATCTGAGTGGAGCCCTGGCTTCTCAGGCAGACCTGGTGTC3690.11879849328740222No Hit
CAGCCTCTGGCATTCTGGTAGCTATGAAGTGCCTGAAAACCAGATGAGAC3670.11815459901484177No Hit
TCAAATGTAGCAAATCAGAAGCCCTCCTAGCAGCTTCCCTCTTTAAGATG3550.11429123337947909No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG3530.11364733910691864No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTACTGGA3480.11203760342551752No Hit
ATATTAACTAAATAGGAAAATACCAGCTTCAGACACAGATCACAAGCCCC3320.10688644924503397No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT3290.10592060783619328No Hit
GTTAGAAGGCTGGCTCCCATGCTGTTCTAACACAGCTTCTAGTTCAGCCA3190.10270113647339106No Hit
TGGGTCCAGTCAGTGAACGTAAGGGTTCTATGATTTCAGTGATGTCTTCA3150.10141334792827018No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)