FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences867288
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT186402.1492283993321712No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG184182.1236313658208115No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT135111.5578446836575623No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT125891.4515362832184926No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG125161.44311924066746No Hit
CAGCTGTGCCATTTGAGGGATGCTCAGGTCGGTTTGGCTCTGCAGCAGGT123651.4257086458016255No Hit
GTAGTCAGTGCATTCTACAACAGCGCGCATGTTAGCAAGTTCATCAACCA117531.3551438507162556No Hit
GCACAAAGGCCCCATTCTGGCACTGACCTATTGCATCCCCAGCCCCAGAC115061.3266642683860495No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACCTCCATGATCTCCAACAAAG111781.2888452278827793No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA106371.2264668714429348No Hit
CAACTGTCAGTCTGCCATTCTTTAAGACAGCTAAGAGGGGAGGATCTAAC104411.2038676887031758No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC101691.1725055575541228No Hit
AGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGG100401.1576316056488734No Hit
GCTGTCTACCTGACCAATCGATGGGGCATTCATTATGACATGAAGATCAG95671.1030937819962918No Hit
CAATGATGAACACTGCGGGCTTCCAGACAATGTTAACACGCTGAATGCCA95401.0999806292719374No Hit
CAGAAATGACATCTACCTGTTACTCACAGAAACCACACACCAAAGGAACA93581.0789956738707327No Hit
GGTTGCTGAGGTGGTGTGATTGCCTTTGGTTCTGGAGAGTCTGGGGGAGA91671.056973000894743No Hit
CTGTGACAGTTCCCAGCGGGTCCGGATATGAATGATAGACTGGACAATGG90401.0423296528950015No Hit
GGATCCGTGGCGAGTTCGTCCACTGTTACAGTGGCCAGTGGTAGGGGAGA84380.9729178773371705No Hit
CCTGGAGTCGATTGATTAGAGCCTAGTCCAGGAGAATGAATTGACACTAA80700.9304867587237458No Hit
AGTGAGGATGAAGAGCTTCCCTGCTTCCAACACTTGTTATTTGGTAAAGT80420.9272583040466372No Hit
GCAGTCTGGGGCCCTTTGGCAGGCCCTGGTTGCCCTGAGGCGGGTGCTGC76090.8773325585042109No Hit
AACAGATATGCTATAGTACAGAAGGCAAAAGAAAAGTGATTACCTTGCTT73040.84216546291428No Hit
TGCAATTTGGTCTCTGCATGTACCACAGAATTGCTGCTTCCCTCAGCCTT72580.8368615730876019No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG71940.829482248111354No Hit
TGGTGGCAAACTCTCCTTCTCAACCAAAAAGAAAGCAATCAACTATTTCA69610.8026168931197019No Hit
GGTCTTTGTGCCCAGACTTCAGCTCCCGTTCTTTCCTGGTCTTCTCCTTG69390.8000802501591168No Hit
GGAAGGTGACGTGAAAGATCACTGTGCAGCAGCAATCTTGACTTCTGGAA67650.780017710379943No Hit
CTGCCCCCTTACCCTCCACACCCCCAGGATTCTTACAGAAAACAAGTGGT65430.7544206768685834No Hit
TAGGAGCCAGGAGAGAATGACCTGTCCCGGTAACTGGAACGGAAGGCAAT65410.7541900729630757No Hit
CACTCAAGAGCACATGTACAGCTGTACCTGATCAGTTCTAGCTGAGCCAG64510.7438128972152273No Hit
TCCACCAATCTCTCAAACCGATCAGCCAATACTGTTTCTGCAAGTGTGTC61830.7129119738771896No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC61790.7124507660661741No Hit
GGCACTTTTCTTCCCCGCTTACTCTAATCACTTACCCACTGAGAACAAGG60310.6953860770586011No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA59650.6877761481768455No Hit
TCTAAAGTATGCAGGTTTTGTTCAAGAAGTGCGGTACCTGCTGAATACAA59450.6854701091217681No Hit
GTTGTAAAATTTCTTCACTCTGAATGTCAGCAAAAACCTTATGTGAATGC58620.6759000470431967No Hit
AAAGAGAGTGAGTCGTTGCGAAGCCCTGTGTTTCAGCTCATTGTGATTAA58620.6759000470431967No Hit
TTATTTTTTGGGGGGAAATTTTTTAGGATCTGATTCTTCTGAAGATACCG57570.6637933420040402No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTGCTTGATTTTCTTACTTGGTCCAA56420.650533617437345No Hit
AGGGCCTATTAGCCATATCTCCCAGCTCTCAACAGGAGGTGGAGAGGCAG55530.6402717436422504No Hit
GTGTACACAGTGTCCACCAAGGTCCTGAGATCCTCATTTCTCTTCCTTGT53650.6185949765245224No Hit
AAGAGGCTTCCAAATTGAGGGTGGGCGCCTGGGAGGGGTGTTTCTGTTGC53040.6115615574065363No Hit
ACCTGTATAGGGTATGCTCTTTGAATAATTACATCAACACAACCAACATT52990.610985047642767No Hit
AGCTTCATCATACCTTTCACTAAGACAAGAATTTAGAAGTGGACAGGAAA52020.5998007582256413No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT51680.5958804918320096No Hit
CACAACCTTGCACTGCTTTATGAAGTGTTTCACCAAACATATTTCGAAGT50660.5841196926511147No Hit
CGACAAGCAACACATCATCGTCCGACTCCAGCTGAGCCTCCGCTGCTGCT50590.5833125789818376No Hit
AGGAAATAATGAGCGAACCCCACTTCCCCACAACGGCACCCCACATGAAA49050.5655560782577413No Hit
GTAGACGATGTAAAGCAAGCACATTGCCGTCACTTATGAAGGAAAGACTA49000.564979568493972No Hit
CATGAGGGTAGTGTTGACAAAGATTAAAACAGAAAATGCTTTATGTTTAG48950.5644030587302027No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC48730.5618664157696175No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG47430.5468771619116141No Hit
GGTTGAACTAAAACGACAATACAATGACCACCATTCAAAATTGAGAGGTC47130.543418103328998No Hit
GTGGATTAACTGACGAAGGATTTTGATTTCACGAATGGCTGTGATTGGGA46330.5341939471086883No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC44800.5165527483373458No Hit
GCAGAATTCCGAAGTTCAGCTGCATGCTGGTTTCCTTCACGACAGGTGTG44620.5144773131877761No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT44410.5120559721799448No Hit
AACATGTTCCAGAGCAGTTGGTAGAGTAAATGCCGAGAATCAAACAGAGT44240.510095838983129No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTCTTGGTGCCATTTATCGTTTTTG44210.5097499331248675No Hit
CCAGATCCTTAACATTGGTCCGTATTTGAAGTCCCACTTTCTCATGGTTA43600.5027165140068812No Hit
TAATCTATATTGATCAGGTGAAGTAAAATGGAGAAAGGAACTGGTAAACA43570.5023706081486197No Hit
ACTTTCAAGTTTCCCCACCCTTTACACAGTTTCCCCGCGGAAAACGCCGC43120.4971820202746954No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC42990.49568309488889506No Hit
CAGTACTTGTCCCTGAGGTAGTTTCTTTTCGAGAAGTTTTGGATGGAGGT42720.4925699421645405No Hit
TTACCCACAGCTCCAAACATCTATGGCTGGTGTGTAACGTTCCTCTCCTA42720.4925699421645405No Hit
GACAGGTTCATGAGTGCTTCCCCCACATTCCTTCAGGTGACTGGTTCTTC42250.4871507503851086No Hit
CCTCAGCTTGCCATCAGGAGTCGTTACCTTTGCTAAGCTCGGAGGGTTTC40910.4717002887160897No Hit
ATCACAATATCTCATTTTGCCCTCTTTGCAACCAGTGCACATTACTGGGT40850.47100847699956644No Hit
GATGTTTTGAGATTTTCAGTTTGTCTACATAAATTATCACTTAAGAGCTT39420.45452029775576275No Hit
CACTGGGCAATTGGTAGGGTAAATCCACAGGGCGCCCGCGGAGGTGCAGA38980.44944701183459246No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG38480.44368191419689884No Hit
TACTGGTGTGGGTTATAGAAGAAACATGAGAGATTTAGTTCAAAAGGAAG38270.4412605731890676No Hit
GTCACTGACAACTGGCTTGTGCAACATTTTGACATGGAAGTCACAGACTA38060.4388392321812362No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC37430.4315752091577423No Hit
CTACCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGATCCCCCACA36620.4222357509846787No Hit
TGTGGTTTAGCCCTCGAAGTAAGAAAGTCAGATATGTTGTGAGTAAAGCT35280.4067852893156598No Hit
GTGTCAATGGCACTGAACCCTGTTTCAGGCCCATCAGTGTTTCCATAAGT34900.40240381511101275No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACAATATCACC33770.3893746944498252No Hit
TATGAGAACACGCAGAGGGAACTTGGCCTCCTACTGCTGCTTTCAGAGAG33500.38626154172547067No Hit
TTATCGCCATCCTTCTGAACTTAAGAAGAGCTCTAAAAAGCCAGTGTACA31980.36873564490688215No Hit
CGTCAGCTATTGAATTACTTTCCAAAAGAGAAATTTCATTGAATTTTTTA30670.3536310890961249No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA30600.35282397542684785No Hit
CTGTGTCACCAAGGGTTTCGTTGGGGCCATGAAGACCACCTTTCCTGAAG29960.3454446504506No Hit
CTGAGGTCGCTCAGTTCCGGAGCTGCAACCCGGAGTCCCAGATGATCGGG29210.33679700399405965No Hit
ATTAGGATGTCTGGCACATGCACAGGACAACCAAGTTCAAGAACCTCTCA28650.3303400946398428No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG28490.3284952633957809No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA28070.3236525813801182No Hit
CTGGGCGCGGGAATCTGTAGAACCATGGTAAAGCGGAAGGGGCCGTGTAG27490.3169650681203937No Hit
ACAAGTTGCAGGACTTTTTGCTGTTTCTTTTTCTACATTTTCATGTACTT27480.3168497661676398No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC27270.3144284251598085No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC27240.3140825193015469No Hit
CAGATGGGCTGGAAGTAAGGAAACATGTAATGATAGGCGGACTCCCAGCA26740.3083174216638533No Hit
AGAAAGAAATATATGGTAAGTTTCAAGAATACATCATACCTGTATAGGGT26310.30335943769543683No Hit
GAAAACTTTTATTGATTTATTTTTTGGGGGGAAATTTTTTAGGATCTGAT26100.3009380966876055No Hit
ACACCGGATATCTGCTTCCTCACAGAGGTGGCGGAAACAGGACATGGCAA24660.28433461549104794No Hit
GCACCTGAGCTCAATGAGATTCATGACCGGTCTGTTTACCTGCTGCCTCC24140.27833891394784666No Hit
AAGTACATATATTTAGCAGATCAGTTAACAGACAAAAGTCAACTTTACAG23040.2656556991449207No Hit
ATGTTACCTTAAGATCAACCACCATGGACTGAACAAGTAGGAAAATGACA22440.25873758197968844No Hit
CGAGATGTTCATTTTCCCCAAACTCACAAAGAGAATTCTTCTCGTAGACG22000.25366429605851804No Hit
AATGCAGATGATTACCTCGCTCTGGCAAATGATTTCATTAAAGATTCAGA21960.25320308824750254No Hit
CGACTCAGAGACCGCTTGCTCAGGAAAGGGCTGCTGGACCGCCTTCGACC21620.2492828218538709No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTAGGCTGCAGCGACTTCGAACT21500.24789919842082447No Hit
CCTGTCATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTA21300.24559315936574702No Hit
TGGATTTACACACTAACCTGGTAGAAATGCGACTAAAGACTGCATTGAAG21040.24259530859414632No Hit
AGAAAAAGTTTCAGTTTTACCAATTTCCATTTTTACGTTTTTAGGTGAAG20730.2390209480587763No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC20700.2386750422005147No Hit
CACGCACACACAAGCAGATTGCTCAGATTACTAGAGAGCTCCGGAGGACG19950.23002739574397432No Hit
AAAAGCCCCTAAACCCCACTTCATTTTCATCTGTTAAATAACCAGAAGCA19490.22472350591729623No Hit
GGAAAAACCAAGTGTGGGAGGGGTGTTGTGATCCCTGATTCTGTAGAACC18970.21872780437409486No Hit
GCCTATAAAATGGCGGCATCGCAGGCGAGGCATCGCAGGTGGGGCATCTC18960.218612502421341No Hit
GTCCATGCTCTTGAAAATAATCATCTACTAACCTTTTTTATAAAACAGTG18720.21584525555524808No Hit
TCTGAGCACCAGTGGAGATTTTGTTAAGCAAACGGTGCCCTCAGAAAATA18720.21584525555524808No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGAATAAGCAGAAACTGCCATGCTC18620.21469223602770937No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT18590.21434633016944776No Hit
GTGTCCTGCTTATTTTTCTCACATTCTTCCGTACTGGCCTGGGAACTCTC18160.20938834620103128No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT16720.19278486500447373No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGAC16480.19001761813838078No Hit
AGAAACAGCTAATAAAAAGTTCTCCAAACTTACTGATTTTTTGTTTTCCT16440.1895564103273653No Hit
TCAGATTGAGACCTCCTTTCCACCACCATGCGATTACTCACGATGTAATC16300.1879421829888111No Hit
ATTGGACCAGTCTACCCTGTAAAAATGTGATTCTTTGCTAGTACCTTTGT15950.18390661464242558No Hit
TCAAAAGGAGATACTTACACAACAGGAAAGACTTTGGGAAACACAACACT15620.1801016502015478No Hit
CCAGTCCTCAGATAAATGGTGAAATAGACTTACTAGCAAGTGGCTCCTTG15590.1797557443432862No Hit
CCATGGGACTGACTTTCTGCTACAAAGAAGACAATCAGCCCTGCATGTAG15440.17802621505197813No Hit
GTCTCTGTATGGCAGCAAATGTGGCCCATGGCCTGCAGCAAAACGTCCTA15120.17433655256385422No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTGTTGA14840.1711080978867458No Hit
CATTCCTTGTTGTGCTCAGTACTGACTTTGGTATGTGTGTTCTGCTGTCC14700.1694938705481916No Hit
ACTAAAATGTTTATCTGATTAATTACCCACCTAATGCAGGAACAAGCAAG13760.15865548698932766No Hit
GTTCTGGAGTACGTATAGCAGTATTTTCTTCTCTTGTGAGCTGGTCTGAA13670.1576177694145428No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT13660.15750246746178892No Hit
GCAGGTGACCAACCACATAACTCGAGACAGGGACAGCGGGAGGCTCAAAC13540.15611884402874246No Hit
AGAACATTTCCTCAGAATTGTCCCAAAAGAGCTAGTTAAGGACAAAGTTG13260.15289038935163407No Hit
ACTTTGTAATTCAACATTCATCGTTGTGTAAATTAAACTTCTCCCATTCC12870.14839361319423305No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT12700.14643347999741724No Hit
CGAATTGGGCTCTTCTGGGAATCAACAGGGATCAGTCAAGGATGGGCAGC12460.14366623313132432No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA12180.1404377784542159No Hit
CTGGGTATGGTGGCACATCTGTAGTCCTAGCTGCTCAGGAGGCTGAGGTG11700.13490328472203003No Hit
ATTACTAAGTCATAAAAATAAACCAGGTAGAATATTTACCTTCACAAACT11680.1346726808165223No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG11580.1335196612889836No Hit
TCTCCAGGTAATAAGGGTGGGAGGGGCAAAGGAGGCAACGTTGAAGTTTT11500.1325972456669526No Hit
ATTGTGTGTTCTTAAAGCAGGCATACTAATTTGAACAGAAACCTTCATTT11450.13202073590318325No Hit
CTTGGCCATAAGAAGCTCCCGAGGGGCTATCATCTTGTTTGGAGCTGTCA11450.13202073590318325No Hit
GTCACCAACCCCATGTCTTTGGAGTGTTTGGACTTATGCCGCTTGTGCTT11010.12694744998201288No Hit
TCCTTCTTTTTCCTACTGAGTTCAGCTCCCAGACTGGAATTAAGTGGAAG10920.12590973240722805No Hit
ACTTTTTCCCTAGCTCTGAAGGTTACGGTAGCAGACCTTTGTGCTTTTAT10590.12210476796635028No Hit
AATTACCTCCAGTTGCTCCTTCTGGGTAAAACCCTTCGTGCTTTTCCCTG10580.12198946601359642No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA10490.12095174843881155No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT10400.11991403086402672No Hit
GCAGTCTGGGGCCCTTTGAAGTGGAGACAGGAAGGCAGTGGGGGTTGTCC10270.11841510547822638No Hit
TCCTTGGATGATGATGCTTTCACGGCTCCATTTCATAGGGATGGAATGCT10220.11783859571445703No Hit
GGACCCCCCGCGAATGCCCACGTGACGTGCAGTCCCCCTGGGGCTGTTCC9900.11414893322633313No Hit
AGTTGAAGAAGCACCCTTTCTGGGCTTAGGCATCTATTAGCAAATTCCTT9760.11253470588777892No Hit
CACTCCACCCCCAAGAATATCATCTAGTGCTGAACAGAAGGTGATTATGA9650.11126638440748632No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA9620.11092047854922471No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG9480.1093062512106705No Hit
ATATGACGTGTCTGCTCCACTTCCATTGAAGGAAGCTTCTCTTTCTCTTA9460.10907564730516277No Hit
AATAGCAACAAGAAAAGATGGAAGAGTACTAAATTTGAGTAATCTAGGAA9230.10642370239182372No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCTGGAAAATAAACATGAC9010.10388705943123852No Hit
AGCATTATGGATCTGTAAGTCTGTTTCTGCTGCAGGTGACCAACCACATA8900.10261873795094595No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG8880.10238813404543819No Hit
GCAATGGTTAAGGTGAACTGGTTCTCATCTACTAGGCAGATTTCTTCAAT8780.10123511451789947No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)