FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299667
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA125544.189316808323906No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC116423.884978993349284No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG102793.4301407896098No Hit
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA80972.701999219133238No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC71962.401332145348003No Hit
GGGAAGCTCTTCATCCTCACTAGACCCAAAACTAGTATCTTCCTTTATTT70602.3559484360974015No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA64762.1610654493154065No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC63572.1213547037211304No Hit
ATATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTG56651.8904317125342462No Hit
TTAAATTTCACTCCACTTACCTACCAGTCAAATGTCCATGGATCATAGAC52651.7569502147383596No Hit
GGGATAAAAGCACAAAGGTCTGCTACCGTACCTTCAGAGCTAGGGAAAAA52211.7422672499808118No Hit
CACTTAATGAGACCAACAGTAACACCACTGACTCTGATCCAGAAGACCAA50211.6755265010828686No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA47801.595103898660847No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT47121.5724120440355462No Hit
CCAAATAACAAGTGTTGGAAGCAGGGAAGCTCTTCATCCTCACTAGATAA46651.5567279680445294No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT44831.495993886547401No Hit
CAGCAGTTACCAGGGCACAGGGTCAGTGCAGTTTCCAGGGGACCAGGACC43431.4492753623188406No Hit
CCTATCTCATCGTCTTATATTGGCTTCACTGTCTTTCAACAGGCCTTGCT41761.3935468369890578No Hit
GGTCCAGCAGCCCTTTCCTGAGCAAGCGGTCTCTGAGTCGGAGTCCACTC41251.3765279460200823No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT39391.314459049544995No Hit
GCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATTGAACTT36471.2170175561539975No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAAA34841.1626238458021738No Hit
GAAGAGACCAAGCAAGTTTTGAGACTCAGTCTGACAGAGTTCTCATTACC34041.1359275462429965No Hit
GTCCTCCAGAGCACAAACCTGTGGCAGATACACACTGGTCCAGCCTTAAC31521.0518342026315877No Hit
TTTTTGTCCAGCCAGCAAATGAGAGTCTGTACCCGACCATTTCACAAAAG29110.9714116002095659No Hit
ATGAGCAAGTTGGGGTGTGGGGGTTGTTGACTTGTAGACCAAGTCTCTGC27470.9166841861132523No Hit
TTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTA26860.8963282576993796No Hit
CCAGTGCAACAGGTGGCTTGGGATCAATAAAAGCTGAGGTGATGGCAGAT26460.882980107919791No Hit
AGTTCCATTTAGGTTGCTGGTGGTGAAGAACATTCAGGCAAGACCAAGTC25520.8516119559377576No Hit
CTCAGTCACACATGCCAGAGATTGCTCAGGAAGCAATGGAGGTAAGGGGA25490.8506108447042884No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACACTCGGTAGCA24290.8105663953655223No Hit
CCAGTGTTGTGTTTCCCAAAGTCTTTCCTGTTGTGTAAGTATCTCCTTTT23360.7795319471279787No Hit
GGTCTCTCATGGCACTGTACTCTTCTTGTCCAGCTGTATCCAGTATGTCC22930.7651826861149209No Hit
TTACCCAGTACCATCAATGCTGTTAACAGTTCTTCATCCTTTTTCCAGCT22610.75450416629125No Hit
CGACACATGACTGCAATGAAATTCAGTCCTGGAAGGAAAAGAAGAAGTAA22310.7444930539565585No Hit
TTAACTACAATTCCCAGCAAGCCGCGGAGCCAACCTGGGCCTCTGCCGGC22090.7371515715777847No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG22080.736817867833295No Hit
TTTTGTGTTTGCACAGTGCCTTTCAGAATGTCCCACCCATAGAGTAGCAG22000.7341482378773773No Hit
ATTACAACCCATCTGAGTGGAGCCCTGGCTTCTCAGGCAGACCTGGTGTC21530.7184641618863605No Hit
CTAATGAGCGGACGGCAAAGAACGCTTTTTCAGACGTGGGGCTCAAGTAT21210.7077856420626896No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG21060.7027800858953439No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA19880.6634030440455572No Hit
ATCTGGTGTTGAAAATTCTAATGACTTTGCATTTTTGAAGGTTTTTCCTT19870.6630693403010676No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT19690.6570626729002527No Hit
GGAAATTGAAGAAATCTGCCTAGTAGATGAGAACCAGTTCACCTTAACCA19080.6367067444863799No Hit
AGTCTTCTCCTAAACCATAGTCTGCTACCGTAGCTGATGATTTGTCCCTG18740.6253608171737295No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA16400.5472741409631358No Hit
AATTTGCATCTGTTTGTCCACATTAGGCTTAGGTTACCACAAGGATCTCC16060.5359282136504854No Hit
GGGATCGATGAAGGACCGGATATCGGGAAGTTCAAAGCGTTCGAATGAGG15480.5165733964700818No Hit
ATGAGCAAGTTGGGGTGTGCTGCGTGTGTGTGTGTATGTGATGTGTGTGT15010.5008893204790651No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAAGCTCTTACCTGTGGGCATGTT14820.49454894933376053No Hit
TATGTCATGATTCATCTTACTAGCCTCAAACATATCTTCTTTGCCAGGAC14390.4801996883207027No Hit
TGGGTCCAGTCAGTGAACGTAAGGGTTCTATGATTTCAGTGATGTCTTCA14110.4708559834749906No Hit
GGATACAGTAGCCAGAATGACGACTCAATCAGTGCTTCGGTTGTTGCTTT13890.4635145010962168No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCAGAGTATTGCCAATCATGTTC13540.45183487003907674No Hit
ACGCCTTCTGTCTGAGCACCCAGAATTAGAACATATCATCTGGACCCTTT13520.45116746255009726No Hit
TCCCCTTTGCTTTGTCTTTTTCCAGCCTCCCCCACTGGCAGGATTGCCAT13510.45083375880560755No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTATCG12840.42847560792479655No Hit
GCCCTCTCAGAAACATCTGCAGGAGGACTTGGGGAAACAAATTCATATGA12770.4261396817133685No Hit
GCCAAACGAAAATTATGGCAGGTTGTTACGAGGCATTGGATGATTCAGAG11570.38609523237460247No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTATC11470.3827581949297053No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC10760.3590652290709354No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT10650.3553944878815485No Hit
TTAAATTTCACTCCACTTACCTACCAAGGGCCTGGTTTAAGGCCCTGTAT10550.3520574504366514No Hit
AACTGAGCGACCTCAGAGCCCTGGCAGCTCCAAGGCGCCTTTGCCAGCAG10130.33804189316808325No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC9520.3176859647542105No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC9410.31401522356482364No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC9200.30700744493053955No Hit
GGGATAATGGGGTTTTCCTGTGTACACTGACGACATTTGCTGCCATACAG8830.29466040638442004No Hit
TGTGATTATTTCTATTTTAGCAACCAAAGGACACAATGAGATTAGATGAA8680.2896548502170743No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA8500.28364818281625936No Hit
TGTTTCAGGAAGGAATGTTCCCAATAGTAGACATAAAAGTCTTCGCACAG7910.2639596618913661No Hit
TCTCCATATCTCTCTCAATTTCAACAAGACAAACAACAGTTGGTATTAGG7390.24660706717790082No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTATCGC7350.24527225219994195No Hit
TTCAACGTTGCCTCCTTTGCCCCTCCCACTCTTATTACCTGGAGATGATG7290.24327002973300363No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC7200.24026669603259618No Hit
GAAGTTCATCCTGTTTTAAGTCACACTTGTGATTTGTTAAATTTTTTAAC7110.23726336233218875No Hit
AGTCTTCAGAATGCCAGAGATATACAGGATATGCGAATTAAGAAGAAACA7060.23559484360974015No Hit
GTGCAGGCTGATTTTCTTTTTCCTGTGTATCTTCTACCAGGTGCTTGGGC6940.23159039867586356No Hit
TATTTAGTAGCCAGGACAGTAGAAGGACTGAAGAGTGAGAGGAGCTCCCA6730.2245826200415795No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT6700.22358150880811034No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCCTGAAAACCAGAT6630.2212455825966823No Hit
GGAGTTGAACCCAGCCGTGACAGCCGCCTTGCTGCAACTTTTATCCCAGC6520.2175748414072954No Hit
AGGTTCAACTACGCGTGTAAATTCTACTGCAAATGCAGAGACACAAGCAA6510.21724113766280573No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA6480.21624002642933654No Hit
ATATATGTCTTCCACCCTTGACTCTCAGGATAGTGCAGCAGGATGCAGCG6420.21423780396239828No Hit
TGACACACTTGCAGAAACAGTATTGGCTGATCGGTTTGAGAGATTGGTGG6320.2109007665175011No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAT6270.20923224779505253No Hit
TGTCATAATGAATGCCCCATCGATTGGTCAGGTAGACAGCAGCAAGCAAT6180.20622891409464503No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTATCGCTG6110.20389298788321703No Hit
CCACTGTAAGTTTATGTCATGGTTGTTTTTTATATTTCAGTCGCCCTTAC5800.1935481718040358No Hit
AGAGGAACGTTACACACCAGCCATAGATGTTTGGAGCTGTGGGTAAGGTT5770.19254706057056667No Hit
CCATTTTCTTCACACCTGTCGTGAAGGAAACCAGCATGCAGCTGAACTTC5630.18787520814771064No Hit
TCCCTTAGAGCTTCCACACATGGACTGGTCATTAATATCATTCACTCTCT5600.18687409691424148No Hit
GCCAAACGAAAATTATGGCAGGTTGTTACGAAGCATTGGATGATTCAGAG5430.18120113325791629No Hit
TCTACCCACTCTCTTTTCAGTGCTACGGTAGCAGAACTGTCAAATGACCA5400.18020002202444713No Hit
TATTTCTGGACAGTCTACGAAAAGCTCTTGCTGGCCATGGAGGAAGTAGG5370.17919891079097797No Hit
GTTAGAAGGCTGGCTCCCATGCTGTTCTAACACAGCTTCTAGTTCAGCCA5360.17886520704648828No Hit
GCTGCCCCAAAGTGTAAAGAAATGCAGAATTCTCTCAATAATGATAAAAA5240.17486076211261165No Hit
TTCAACGTTGCCTCCTTTGCCCCTCCCACCCTTATTACCTGGAGATGATG5080.1695215022007762No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC5080.1695215022007762No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTATCG4990.16651816850036874No Hit
GGTAGGATGTGATATTCCTTCTAGTGGAATCGCAGTGTGGTGCTCTGTTT4880.16284742731098187No Hit
AAGGGAAAAGGATGGATTAGGGTACACTGACGGACATGGTTCTACTACCC4750.15850927863261555No Hit
TATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTGA4640.15483853744322867No Hit
ATTTCTCTTCTGACCTCTCAATTTTACCTTTTCTAGATGTATTCTTGGGG4620.15417112995424923No Hit
TGAACTTATATATTTGACACAACCCAGCAGTTCGTAAGTAGTTCACAGAA4620.15417112995424923No Hit
GTGCACATTCAAAAAAGAAATTATCCACACTGACGACATGGTTCTTCAGT4560.1521689074873109No Hit
CTGTATGTAGTCGGTGCTGTGACTTGTTTGTGCTCATCTCTGTTCTGTAG4520.15083409250935204No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG4300.1434926101305783No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT4270.14249149889710913No Hit
TTGTGGCACCAAATACGAAACACCCATAAAGAAAAAAGAACTGAATTCTC4070.1358174240073148No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTATCGCTGA4020.1341489052848662No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT3790.12647371916160272No Hit
TCAGAGACCAGACTCCAGAGCACACTGACGACATGGTTCTACATCAATCA3780.126140015417113No Hit
ACCTCCATCCAAAACTTCTCGAAAAGAAACTACCTCAGGGACAAGTACTG3740.12480520043915413No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC3710.12380408920568498No Hit
GTAACTTGATTTGCTGTTGTATTTGCTTAGGAAGAGCAGCACTTCAGAAA3670.1224692742277261No Hit
GACCTTTTCCTCTGCCCTTATCATCGCTTTTCAGTGATGGAGGAAATGTT3650.12180186673874667No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCGATATATGT3620.12080075550527754No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGATTGACAGTTGGTCAGA3560.11879853303833922No Hit
CCAAGGAACATCTTCAGTATCTCTAGGATTCTCTGAGCATGGCAGTTTCT3550.11846482929384951No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA3500.11679631057140093No Hit
TCAATGGCAAGTAAGTTATTTATGGTCGTTTTTAAGGATAAGCTGTTAAC3490.11646260682691122No Hit
AGCGTTATACCTTTGCCCTGAGCTTCCTGCTGCTGCAGGCCGCCTTTTTC3350.11179075440405517No Hit
CTGTGGTGCCACCTAAGCTCTTAAGTGATAATTTATGTAGACAAACTGAA3230.10778630947017856No Hit
ACTCACTCCATTGTTCTTGCTTTTGCTTTGTTTTCAGCCGACTTTGTGGT3200.10678519823670943No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG3200.10678519823670943No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT3180.10611779074772998No Hit
CTACTCTTTAGCTTCCTACCTAAGAATAAAAATGGGATTGTTTGCACTAA3150.10511667951426083No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTATCGCT3140.10478297576977111No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT3080.1027807533028328No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)