FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299667
Sequences flagged as poor quality0
Sequence length150
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT124804.164622731231668No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG104013.470852646437546No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT99743.3283611475404364No Hit
GTAGTCAGTGCATTCTACAACAGCGCGCATGTTAGCAAGTTCATCAACCA79282.6456032863144756No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG69422.316571394247615No Hit
GGTGAAATAAAGGAAGATACTAGTTTTGGGTCTAGTGAGGATGAAGAGCT69322.3132343568027176No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC66512.219463604601107No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA63262.1110098876419494No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA60562.0209098766297258No Hit
ATGATCCATGGACATTTGACTGGTAGGTAAGTGGAGTGAAATTTAATGTA52121.7392639162804047No Hit
TCTGGATCAGAGTCAGTGGTGTTACTGTTGGTCTCATTAAGTGTGTAGAA49841.6631794625367493No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG46101.538374262097595No Hit
TTATTTTTTGGGGGGAAATTTTTTAGGATCTGATTCTTCTGAAGATACCG45531.5193531486616811No Hit
AGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGG45051.5033353689261748No Hit
ACTTTTTCCCTAGCTCTGAAGGTACGGTAGCAGACCTTTGTGCTTTTATC44781.4943253678249524No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC44351.4799761068118946No Hit
CAGCTGTGCCATTTGAGGGATGCTCAGGTCGGTTTGGCTCTGCAGCAGGT42111.405226468046198No Hit
CAATGATGAACACTGCGGGCTTCCAGACAATGTTAACACGCTGAATGCCA40181.3408216453596826No Hit
TGCAATTTGGTCTCTGCATGTACCACAGAATTGCTGCTTCCCTCAGCCTT39411.3151264570339745No Hit
CAACTGTCAGTCTGCCATTCTTTAAGACAGCTAAGAGGGGAGGATCTAAC35631.1889864416168614No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT35391.1809775517491081No Hit
TAGGAGCCAGGAGAGAATGACCTGTCCCGGTAACTGGAACGGAAGGCAAT32611.0882079107809668No Hit
GGAAGGTGACGTGAAAGATCACTGTGCAGCAGCAATCTTGACTTCTGGAA30111.0047819746585376No Hit
TGGTGGCAAACTCTCCTTCTCAACCAAAAAGAAAGCAATCAACTATTTCA28790.9607330803858951No Hit
ACAAGTCAACAACCCCCACACCCCAACTTGCTCATTGTAGAACCATGTCG27390.9140145561573345No Hit
GGTTGCTGAGGTGGTGTGATTGCCTTTGGTTCTGGAGAGTCTGGGGGAGA26560.8863171453646882No Hit
TGCCTGAATGTTCTTCACCACCAGCAACCTAAATGGAACTTGTAGAACCA25310.8446041773034735No Hit
AACAGATATGCTATAGTACAGAAGGCAAAAGAAAAGTGATTACCTTGCTT25000.8342593612242923No Hit
CAGAAATGACATCTACCTGTTACTCACAGAAACCACACACCAAAGGAACA24100.8042260242202177No Hit
CTGCCCCCTTACCCTCCACACCCCCAGGATTCTTACAGAAAACAAGTGGT22630.7551715737802294No Hit
AGTGAGGATGAAGAGCTTCCCTGCTTCCAACACTTGTTATTTGGTAAAGT22620.7548378700357397No Hit
GGATCCGTGGCGAGTTCGTCCACTGTTACAGTGGCCAGTGGTAGGGGAGA22480.7501660176128837No Hit
CGACAAGCAACACATCATCGTCCGACTCCAGCTGAGCCTCCGCTGCTGCT21220.7081193458071793No Hit
ATCACAATATCTCATTTTGCCCTCTTTGCAACCAGTGCACATTACTGGGT20710.6911004548382038No Hit
AAAGAGAGTGAGTCGTTGCGAAGCCCTGTGTTTCAGCTCATTGTGATTAA20600.6874297136488169No Hit
GTGTACACAGTGTCCACCAAGGTCCTGAGATCCTCATTTCTCTTCCTTGT20580.6867623061598375No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACCTCCATGATCTCCAACAAAG20560.6860948986708579No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT20410.6810893425035123No Hit
GTAGACGATGTAAAGCAAGCACATTGCCGTCACTTATGAAGGAAAGACTA20090.6704108226798413No Hit
ACTTTCAAGTTTCCCCACCCTTTACACAGTTTCCCCGCGGAAAACGCCGC19970.6664063777459647No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT19760.6593985991116806No Hit
AGGGCCTATTAGCCATATCTCCCAGCTCTCAACAGGAGGTGGAGAGGCAG19600.6540593391998452No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT19490.6503885980104582No Hit
CAGACAGGGACAAATCATCAGCTACGGTAGCAGACTATGGTTTAGGAGAA18420.6146822973500585No Hit
CTGTGACAGTTCCCAGCGGGTCCGGATATGAATGATAGACTGGACAATGG18280.6100104449272026No Hit
GTTGTAAAATTTCTTCACTCTGAATGTCAGCAAAAACCTTATGTGAATGC16300.5439371035182385No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT16230.5416011773068106No Hit
ACAAGTCAACAACCCCCACACACACCACACACACATCACATACACACACA15230.5082308028578388No Hit
GGTCTTTGTGCCCAGACTTCAGCTCCCGTTCTTTCCTGGTCTTCTCCTTG15130.5048937654129417No Hit
TCCACCAATCTCTCAAACCGATCAGCCAATACTGTTTCTGCAAGTGTGTC14480.48320302202111015No Hit
CCAGATCCTTAACATTGGTCCGTATTTGAAGTCCCACTTTCTCATGGTTA13620.4545044999949945No Hit
TCTAAAGTATGCAGGTTTTGTTCAAGAAGTGCGGTACCTGCTGAATACAA13590.4535033887615253No Hit
CAGTACTTGTCCCTGAGGTAGTTTCTTTTCGAGAAGTTTTGGATGGAGGT13230.4414900539598955No Hit
CTGAGTAAGTGGCAAGAAAATTACCTGCGTGCTGCATCAAAGTTGCTTTT12980.43314746034765256No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA12970.4328137566031629No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTGCTTGATTTTCTTACTTGGTCCAA12420.41446005065622843No Hit
AGGAAATAATGAGCGAACCCCACTTCCCCACAACGGCACCCCACATGAAA12380.41312523567826953No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG11970.3994433821541911No Hit
CATGAGGGTAGTGTTGACAAAGATTAAAACAGAAAATGCTTTATGTTTAG11770.39276930726439685No Hit
TGTGGTTTAGCCCTCGAAGTAAGAAAGTCAGATATGTTGTGAGTAAAGCT11520.3844267136521539No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG10690.3567293028595074No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC10620.35439337664807935No Hit
GGTTGAACTAAAACGACAATACAATGACCACCATTCAAAATTGAGAGGTC10110.3373744856791038No Hit
CACTGGGCAATTGGTAGGGTAAATCCACAGGGCGCCCGCGGAGGTGCAGA9850.3286981883223712No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG9570.31935448347665907No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC9180.30634003744156013No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG9000.30033337004074523No Hit
GTCTCTGTATGGCAGCAAATGTCGTCAGTGTACACAGGAAAACCCCATTA8740.2916570726840126No Hit
GCAGAATTCCGAAGTTCAGCTGCATGCTGGTTTCCTTCACGACAGGTGTG8660.28898744272809485No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT8520.2843155903052388No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT7330.2446048447109625No Hit
AGCTTCATCATACCTTTCACTAAGACAAGAATTTAGAAGTGGACAGGAAA7260.2422689184995345No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTAGGCTGCAGCGACTTCGAACT7120.23759706607667844No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG7060.23559484360974015No Hit
CTACCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGATCCCCCACA6900.2302555836979047No Hit
TATGAGAACACGCAGAGGGAACTTGGCCTCCTACTGCTGCTTTCAGAGAG6870.2292544724644355No Hit
AGAAAAAGTTTCAGTTTTACCAATTTCCATTTTTACGTTTTTAGGTGAAG6860.2289207687199458No Hit
GCTGTCTACCTGACCAATCGATGGGGCATTCATTATGACATGAAGATCAG6440.21490521145137767No Hit
GGCACTTTTCTTCCCCGCTTACTCTAATCACTTACCCACTGAGAACAAGG6310.21056706277301135No Hit
ATTAGGATGTCTGGCACATGCACAGGACAACCAAGTTCAAGAACCTCTCA6290.20989965528403193No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA6250.20856484030607308No Hit
GTGTCAATGGCACTGAACCCTGTTTCAGGCCCATCAGTGTTTCCATAAGT6240.20823113656158335No Hit
GTCCATGCTCTTGAAAATAATCATCTACTAACCTTTTTTATAAAACAGTG6240.20823113656158335No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA6210.20723002532811421No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC6140.2048940991166862No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT6100.20355928413872734No Hit
AAAAGCCCCTAAACCCCACTTCATTTTCATCTGTTAAATAACCAGAAGCA6010.20055595043831984No Hit
TTATCGCCATCCTTCTGAACTTAAGAAGAGCTCTAAAAAGCCAGTGTACA5440.181534837002406No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACAATATCACC5410.18053372576893684No Hit
TTACCCACAGCTCCAAACATCTATGGCTGGTGTGTAACGTTCCTCTCCTA5380.1795326145354677No Hit
GGGCTGTAGAGAATGATTGATGTAGAACCATGTCGTCAGTGTGCTCTGGA5380.1795326145354677No Hit
TGGTCATTTGACAGTTCTGCTACCGTAGCACTGAAAAGAGAGTGGGTAGA5320.1775303920685294No Hit
TAATCTATATTGATCAGGTGAAGTAAAATGGAGAAAGGAACTGGTAAACA5300.17686298457954996No Hit
TGGATTTACACACTAACCTGGTAGAAATGCGACTAAAGACTGCATTGAAG5260.1755281696015911No Hit
GATGTTTTGAGATTTTCAGTTTGTCTACATAAATTATCACTTAAGAGCTT5260.1755281696015911No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTCTTGGTGCCATTTATCGTTTTTG5170.17252483590118364No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA4990.16651816850036874No Hit
ATGTTACCTTAAGATCAACCACCATGGACTGAACAAGTAGGAAAATGACA4930.16451594603343045No Hit
ACACCGGATATCTGCTTCCTCACAGAGGTGGTGGAAACAGGACATGGCAA4800.1601777973550641No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA4730.1578418711436361No Hit
CACTCAAGAGCACATGTACAGCTGTACCTGATCAGTTCTAGCTGAGCCAG4660.15550594493220807No Hit
CCAAAGCAGAAAGGGTAGTAGAACCATGTCCGTCAGTGTACCCTAATCCA4650.15517224118771836No Hit
CAGAAGATCCAGAAGGCACTGAAGAACCATGTCGTCAGTGTGGATAATTT4500.15016668502037261No Hit
TCATTCAGAAAACAAACAGAGCACCACACTGCGATTCCACTAGAAGGAAT4500.15016668502037261No Hit
CTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG4480.1494992775313932No Hit
GAAAACTTTTATTGATTTATTTTTTGGGGGGAAATTTTTTAGGATCTGAT4390.14649594383098571No Hit
TATGAAGTAGCCTGCTATAATCGATCAAACTAACCCTAACTATCAAGATG4290.14315890638608855No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT4220.14082298017466052No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG4220.14082298017466052No Hit
CGTCAGCTATTGAATTACTTTCCAAAAGAGAAATTTCATTGAATTTTTTA4100.13681853524078394No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC3940.13147927532894846No Hit
AGAAACAGCTAATAAAAAGTTCTCCAAACTTACTGATTTTTTGTTTTCCT3910.13047816409547933No Hit
GCACAAAGGCCCCATTCTGGCACTGACCTATTGCATCCCCAGCCCCAGAC3700.12347038546119526No Hit
CAGATGGGCTGGAAGTAAGGAAACATGTAATGATAGGCGGACTCCCAGCA3630.12113445924976725No Hit
AAGAGGCTTCCAAATTGAGGGTGGGCGCCTGGGAGGGGTGTTTCTGTTGC3560.11879853303833922No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT3450.11512779184895235No Hit
AGAAAGAAATATATGGTAAGTTTCAAGAATACATCATACCTGTATAGGGT3440.11479408810446262No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG3420.11412668061548319No Hit
GACCCATTCCACCGGCAGTTCGAAAAAGGCGGCCTGCAGCAGCAGGAAGC3410.11379297687099346No Hit
CATTCCTTGTTGTGCTCAGTACTGACTTTGGTATGTGTGTTCTGCTGTCC3240.10812001321466827No Hit
CGAGATGTTCATTTTCCCCAAACTCACAAAGAGAATTCTTCTCGTAGACG3230.10778630947017856No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC3190.1064514944922197No Hit
CTGGGTATGGTGGCACATCTGTAGTCCTAGCTGCTCAGGAGGCTGAGGTG3170.10578408700324027No Hit
AACATGTTCCAGAGCAGTTGGTAGAGTAAATGCCGAGAATCAAACAGAGT3170.10578408700324027No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC3120.10411556828079167No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT3120.10411556828079167No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT3100.10344816079181224No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)