FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1024871
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC996299.721125878281267No Hit
GAAAAACAATTTATATCTGTCAGTGAATCCACTAGGACTGCTCCCACCAG629176.139016520127899No Hit
ATAATGATGATGCCAAACGACAAAGAGTTACTGCTATTCTTGACAAGCTG490334.784309439919756No Hit
TTTTCCTGGCTTTGCTTACGACAACGTCTCCGCAGTCTATATCTATAACT409613.9966981210318178No Hit
TCAACTTGAGGGAGGGAGCTTTACCTTTCTGTCCTGGGATTCTCTTGCTC405633.957863965318562No Hit
TGCAATTCTGAGGTGTTAAAGGGAGGAGGGGAGAAATAGTATTATACTTA345513.371253552886168No Hit
GATAAATTCAGTTTTTATTCTCAGTTATTCAGTGACTTGTTTAAACAGTG310573.0303325979562303No Hit
TCGAATCAAGCACTAATACCAAGAGATCATAGTAAAAATTTTACTAGTGG280742.739271576617935No Hit
TCAGCTCACTTCAAGAAATCTCCATGTATGCACAAGTAACTCATCAGAAG269482.6294040908563123No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTACA263182.567932939852918No Hit
GAGTTGAACAGTGTGTTAGGAATATTAACTTGGAGGAAAACAGACAAAAG224182.1873972431652375No Hit
TCGAAAGCATCATAGTTACTGGAAATTTGAGTTTTCCATTTATAAATACA213272.0809448213482478No Hit
GTCCCAAATGGCAATTGTTCCAGAAGTCAAGATTGCTGCTGCACAGTGAT201291.964052061186237No Hit
CTTGTGTTGAAATTGTAAATACCTTGGCATTAGATAATCAAAAGAAACTG187041.825010172011892No Hit
CTACTACTATATGTGCATTGAGAGTTTTTATACTAGTGATTTTAAACTAT184401.7992508325438032No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAAA53350.5205533184176351No Hit
GCTTAGATTCCTCTTTGGAGTCAGTGTACCTCTCACAATATTAAATGCAC51590.5033804254389089No Hit
GATCACGTTAATTCCCTATCTTGCTGCAGAAACTCAGAGGATTTCCTCAT44170.4309810698126886No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACAAGGTAATTAAATGTGGTAC25340.2472506295914315No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA24650.24051807495772637No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAAC23420.2285165645237303No Hit
TCGAATCAAGCACTAATACCAAGAGGGGGAAGAGGGAAGACCAAGTCTCT19660.19182902043281544No Hit
TCGGCTATAAAAAAGATAATGGAAAGGGATGACACAGCTGCAAAAACACT19170.1870479309103292No Hit
CCTATCTCATCGTCTTATATTGGCTTCACTGTCTTTCAACAGGCCTTGCT18160.17719303209867387No Hit
GCTTAGATTCCTCTTTGGAGTCAAATTCACCATCTTCTTTTTCTGCCTCT18040.17602215303194255No Hit
ACCTCCATCCAAAACTTCTCGAAAAGAAACTACCTCAGGGACAAGTACTG17570.17143621002057818No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT17230.1681187193315061No Hit
CGACACATGACTGCAATGAAATTCAGTCCTGGAAGGAAAAGAAGAAGTAA16810.16402064259794646No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG16710.16304491004233704No Hit
ATCTCCTGGTTATTCTCAGTTTTCTTTACCAGTGCAAGACCAAGTCTCTG16680.1627521902756542No Hit
GGTCCAGCAGCCCTTTCCTGAGCAAGCGGTCTCTGAGTCGGAGTCCACTC16130.1573856612198023No Hit
ATTTAACACCTAGCCAAAAGGCAGACCAAGTCTCTGCTACCGTACACTGC15140.14772590891926887No Hit
TGAGTCAGAAAATGAACAAGATTCCTCATTACTTGACTTTTTAAATGATG14790.1443108449746358No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA14710.14353025893014829No Hit
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA14040.13699285080756504No Hit
TTACCCAGTACCATCAATGCTGTTAACAGTTCTTCATCCTTTTTCCAGCT13550.1322117612850788No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAAG13520.13191904151839598No Hit
CCTCTCTCCTTCTTCCCCAAGTACACACACAAACCTGCCAATGCCAGTGG13430.13104088221834748No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCCTGAAAACCAGAT13360.13035786942942088No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGATATGCAGAATCCAAATTA13350.13026029617385992No Hit
TCTGGGGTATCAGGTAGGTGTCCAGCTCCTGGCACTGGTAGAGTGCTACA13330.13006514966273805No Hit
TTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTA13330.13006514966273805No Hit
GCTTAGATTCCTCTTTGGAGTCAAATTCACCATCTTCAGACCAAGTCTCT13280.12957728338493332No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAGA13240.12918699036268955No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT13100.12782096478483632No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG12940.12625979269586124No Hit
TGACAATGAGATTCATCAGTTTAACAAAAACAACTCCAATCAAGCAGTAG12940.12625979269586124No Hit
TGACACACTTGCAGAAACAGTATTGGCTGATCGGTTTGAGAGATTGGTGG12830.12518648688469086No Hit
GTCCTCCAGAGCACAAACCTGTGGCAGATACACACTGGTCCAGCCTTAAC12680.12372288805127671No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC12670.12362531479571574No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG12620.12313744851791103No Hit
CTGGCTGAGCACATAGAGCATGAACAACAGAAACTACCTGCTGCCACCAG12490.12186899619561876No Hit
TGTTTCAGGAAGGAATGTTCCCAATAGTAGACATAAAAGTCTTCGCACAG12370.12069811712888744No Hit
CCAGTGCAACAGGTGGCTTGGGATCAATAAAAGCTGAGGTGATGGCAGAT12240.11942966480659517No Hit
CGATGCGCGGGAAGACGTTCCGCTTTGAAATGCAGCGGGATTTGGTGAGT12200.1190393717843514No Hit
CCAAATAACAAGTGTTGGAAGCAGGGAAGCTCTTCATCCTCACTAGATAA11940.11650246713976685No Hit
CTCAGTCACACATGCCAGAGATTGCTCAGGAAGCAATGGAGGTAAGGGGA11910.11620974737308404No Hit
CCAGTGTTGTGTTTCCCAAAGTCTTTCCTGTTGTGTAAGTATCTCCTTTT11860.1157218810952793No Hit
GCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATTGAACTT11790.1150388683063527No Hit
GACCCAAAACCCAAAATGGCAATTGTTTACACTGCAAGAAGTCTAGACCA11710.11425828226186514No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA11600.11318497645069477No Hit
TGGGTCCAGTCAGTGAACGTAAGGGTTCTATGATTTCAGTGATGTCTTCA11540.1125995369173291No Hit
CTGTGGTGCCACCTAAGCTCTTAAGTGATAATTTATGTAGACAAACTGAA11490.11211167063952439No Hit
TCTGATCAAAGAACAGGAGAGTTCCCAGGCCAGTACGGAAGAATGTGAGA11460.11181895087284156No Hit
TTTTGTGCGAAGGAAACCGATGGGGATCGGAACCGTAGCGGTTGAGCTGC11310.11035535203942741No Hit
CCCTCAGATGTTATTTTCCAAGCAGGATTTTAATTCAAACCATAATTTAA11260.10986748576162267No Hit
GGAAATTGAAGAAATCTGCCTAGTAGATGAGAACCAGTTCACCTTAACCA11230.10957476599493986No Hit
CTAATGAGCGGACGGCAAAGAACGCTTTTTCAGACGTGGGGCTCAAGTAT11220.10947719273937892No Hit
TATGTCATGATTCATCTTACTAGCCTCAAACATATCTTCTTTGCCAGGAC11150.10879417995045229No Hit
GAAGAGACCAAGCAAGTTTTGAGACTCAGTCTGACAGAGTTCTCATTACC11120.10850146018376947No Hit
GATAAATTCAGTTTTTATTCTCAGTTATTCAGTTTTTATTCTCAGTTTTC11070.10801359390596477No Hit
AATTTGCATCTGTTTGTCCACATTAGGCTTAGGTTACCACAAGGATCTCC11040.10772087413928191No Hit
GGGATCGATGAAGGACCGGATATCGGGAAGTTCAAAGCGTTCGAATGAGG10950.10684271483923342No Hit
GCCTACCAGTCCAGCACCCGGTCACCGAGCCCCTACAGTAGGCGACAGAG10790.10528154275025832No Hit
GCCCTCTCAGAAACATCTGCAGGAGGACTTGGGGAAACAAATTCATATGA10750.10489124972801456No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG10740.1047936764724536No Hit
ATTACAACCCATCTGAGTGGAGCCCTGGCTTCTCAGGCAGACCTGGTGTC10730.10469610321689267No Hit
GGCAGTTCTCTTGAGTCAGCTGATGAAAACCCAAGAGCCAGCAGGCAGTC10700.10440338345020983No Hit
TTCAACGTTGCCTCCTTTGCCCCTCCCACCCTTATTACCTGGAGATGATG10620.10362279740572228No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT10570.10313493112791756No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG10560.10303735787235663No Hit
TCCCCTTTGCTTTGTCTTTTTCCAGCCTCCCCCACTGGCAGGATTGCCAT10530.10274463810567377No Hit
TTGTGGCACCAAATACGAAACACCCATAAAGAAAAAAGAACTGAATTCTC10510.10254949159455189No Hit
GGGAAAAGAACAGGCTTCACCTAGCCAAAAGGCAGACCAAGTCTCTGCTA10290.10040287997221113No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)