FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1024871
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG990559.665118829589286No Hit
GTGTCCTGCTTATTTTTCTCACATTCTTCCGTACTGGCCTGGGAACTCTC558975.454052266090073No Hit
ATGTGCACAAAGGAGATACTAGATAAATATTTGAGCAAACTCAATACCTG469574.581747361375236No Hit
ATATGACGTGTCTGCTCCACTTCCATTGAAGGAAGCTTCTCTTTCTCTTA396703.8707310481026393No Hit
GGTGGCAGCAGGTAGTTTCTGTTGTTCATGCTCTATGTGCTCAGCCAGTT379483.7027099020266943No Hit
GGACAGCACTTCCTGATTTTGTTTTCAACTTCTAATCCTTTGAGTGTTTT330193.2217713253668028No Hit
TGCACTGGTAAAGAAAACTGAGAATAACCAGGAGATAACAATTCTTCATT271812.6521386594020124No Hit
GAACAAGTGTTTTTGCAGCTGTGTCATCCCTTTCCATTATCTTTTTTATA270042.6348681931677254No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG256602.503729737693817No Hit
TGAAGTGCATTTAATATTGTGAGAGGTCTTTGAAAACCCCATCTTGAGCA242622.3673223264196177No Hit
AGTTACAGCTACTGCTTGATTGGAGTTGTTTTTGTTAAACTGATGAATCT207262.0223032947561204No Hit
AGACTTCTTGCAGTGTAAACAATTGTTTATTTCATTTACACAAGGTGAAA203091.9816152471872068No Hit
TTTGTTCCTGTTGCTGGTTTTGGGAACATGGTTTTGACCTTTTTTTTTTT190301.8568190533247602No Hit
GCCTTTTGGCTAGGTGTTAAATTATGGTTTGAATTAAAATCCTGCTTGGA179991.756221026841427No Hit
ACAGAGGACTTACCATGACTTGCAGCTTCTCTTTGATTTGTGTTTTCACT171711.6754303712369654No Hit
TGAAGTGCATTTAATATTGTGAGAGGTACACTGACTCCAAAGAGGAATCT49410.4821094557266231No Hit
GGGGACTCAAAAAGGGGAAATCCTTACTAGAACAGTAAGAAGCAGCGCCT43990.4292247512125916No Hit
TGCACGTACCACATTTAATTACCTTGTCTTCTCTTGGTATTAGTGCTTGA24440.23846903659094656No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG23410.22841899126816936No Hit
TCCCTCTTCCCCCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGT19240.1877309436992558No Hit
GCACAAAGGCCCCATTCTGGCACTGACCTATTGCATCCCCAGCCCCAGAC18320.17875420418764898No Hit
CAGCTGTGCCATTTGAGGGATGCTCAGGTCGGTTTGGCTCTGCAGCAGGT17610.17182650304282196No Hit
TGCTTTTGTCTGTTTTCCTCCAAGTTAATATTCCTAACACACTGTTCAAC17440.17016775769828593No Hit
GGAAGGTGACGTGAAAGATCACTGTGCAGCAGCAATCTTGACTTCTGGAA17120.16704541352033572No Hit
TGCACTGGTAAAGAAAACTGAGAATAACCAGGAGATTGTAGAACCATGTC16750.16343520306458081No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACCTCCATGATCTCCAACAAAG16610.16206917748672758No Hit
GGATCCGTGGCGAGTTCGTCCACTGTTACAGTGGCCAGTGGTAGGGGAGA16520.1611910181866791No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT16380.1598249926088259No Hit
CAATGATGAACACTGCGGGCTTCCAGACAATGTTAACACGCTGAATGCCA15740.1535803042529255No Hit
GCCTTTTGGCTAGGTGTTAAATTGTAGAACCATGTCGTCAGTGTAGATCT15190.14821377519707357No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT14730.1437254054412702No Hit
TGCACTGGTAAAGAAAACTGAGAATAAAAACTGAATAACTGAGAATAAAA14360.14011519498551525No Hit
GGTTGCTGAGGTGGTGTGATTGCCTTTGGTTCTGGAGAGTCTGGGGGAGA14010.13670013104088222No Hit
AGTTATAGATATAGACTGCGGAGACGTTGTCGTAAGCAAAGCCAGGAAAA13870.135334105463029No Hit
CCTGGAGTCGATTGATTAGAGCCTAGTCCAGGAGAATGAATTGACACTAA13630.13299234732956636No Hit
GAAGATGGTGAATTTGACTCCAAAGAGGAATCTAAGCTGTAGAACCATGT13350.13026029617385992No Hit
GTAGTCAGTGCATTCTACAACAGCGCGCATGTTAGCAAGTTCATCAACCA13250.12928456361825047No Hit
GCAGTCTGGGGCCCTTTGGCAGGCCCTGGTTGCCCTGAGGCGGGTGCTGC13130.12811368455151917No Hit
GTGTACACAGTGTCCACCAAGGTCCTGAGATCCTCATTTCTCTTCCTTGT13090.1277233915292754No Hit
GGTGGGAGCAGTCCTAGTGGATTCACTGACAGATATAAATTGTTTTTCTG13000.1268452322292269No Hit
GCTGTCTACCTGACCAATCGATGGGGCATTCATTATGACATGAAGATCAG12830.12518648688469086No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA12720.12411318107352048No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT12660.1235277415401548No Hit
TACCATTTATAGCCTGTGAAAGTTGAGTTTCATCATTTAAAAAGTCAAGT12620.12313744851791103No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG12550.12245443572898443No Hit
GGTGGCAGCAGGTAGTTTCTGTTGTTCATGCTCTATGTGCTCAGCCAGTG12410.12108841015113121No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG12270.11972238457327801No Hit
CAACTGTCAGTCTGCCATTCTTTAAGACAGCTAAGAGGGGAGGATCTAAC12240.11942966480659517No Hit
AGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGG12170.11874665201766857No Hit
GGCACTTTTCTTCCCCGCTTACTCTAATCACTTACCCACTGAGAACAAGG12130.11835635899542479No Hit
AACAGATATGCTATAGTACAGAAGGCAAAAGAAAAGTGATTACCTTGCTT12080.11786849271762008No Hit
CAGAAATGACATCTACCTGTTACTCACAGAAACCACACACCAAAGGAACA12080.11786849271762008No Hit
CCTCAGCTTGCCATCAGGAGTCGTTACCTTTGCTAAGCTCGGAGGGTTTC12050.11757577295093723No Hit
TGGTTAAGATCATTTGGTAACATTTGTATGTCAAATACATAGTGAATATA12010.11718547992869345No Hit
TGGTGGCAAACTCTCCTTCTCAACCAAAAAGAAAGCAATCAACTATTTCA11970.1167951869064497No Hit
AGGCTTGCTCAGTTTCTTTTGATTATCTAATGCCAAGGTATTTACAATTT11930.11640489388420593No Hit
GTTATCAGCTTGTCAAGAATAGCAGTAACTCTTTGTCGTTTGGCATCATC11840.11552673458415744No Hit
ATCACAATATCTCATTTTGCCCTCTTTGCAACCAGTGCACATTACTGGGT11620.11338012296181665No Hit
AGACTTCTTGCAGTGTAAACAATTGCCATTTTGGGTTTTGGGTCTGTAGA11610.11328254970625572No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC11530.11250196366176816No Hit
CCAGATCCTTAACATTGGTCCGTATTTGAAGTCCCACTTTCTCATGGTTA11530.11250196366176816No Hit
AGCTTCATCATACCTTTCACTAAGACAAGAATTTAGAAGTGGACAGGAAA11460.11181895087284156No Hit
GCACCTGAGCTCAATGAGATTCATGACCGGTCTGTTTACCTGCTGCCTCC11260.10986748576162267No Hit
GGTCTTTGTGCCCAGACTTCAGCTCCCGTTCTTTCCTGGTCTTCTCCTTG10920.10654999507255059No Hit
TCTAAAGTATGCAGGTTTTGTTCAAGAAGTGCGGTACCTGCTGAATACAA10770.10508639623913643No Hit
CTGTGACAGTTCCCAGCGGGTCCGGATATGAATGATAGACTGGACAATGG10770.10508639623913643No Hit
ACAAGTTGCAGGACTTTTTGCTGTTTCTTTTTCTACATTTTCATGTACTT10750.10489124972801456No Hit
CGACAAGCAACACATCATCGTCCGACTCCAGCTGAGCCTCCGCTGCTGCT10660.10401309042796605No Hit
CTGAGGTCGCTCAGTTCCGGAGCTGCAACCCGGAGTCCCAGATGATCGGG10620.10362279740572228No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC10620.10362279740572228No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC10610.10352522415016134No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT10410.10157375903894247No Hit
CATGAGGGTAGTGTTGACAAAGATTAAAACAGAAAATGCTTTATGTTTAG10410.10157375903894247No Hit
TCCACCAATCTCTCAAACCGATCAGCCAATACTGTTTCTGCAAGTGTGTC10350.1009883195055768No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTCTTGGTGCCATTTATCGTTTTTG10350.1009883195055768No Hit
TAGGAGCCAGGAGAGAATGACCTGTCCCGGTAACTGGAACGGAAGGCAAT10340.10089074625001584No Hit
CGTCAGCTATTGAATTACTTTCCAAAAGAGAAATTTCATTGAATTTTTTA10270.10020773346108924No Hit
GCCTTTTGGCTAGGTGAAGCCTGTTCTTTTCCCTGTAGAACCATGTCGTC10260.10011016020552832No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)