FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316546
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTTAGTGAATGTGATTGATGGTACTTTAATTTTGTCACTTTGTGTTT16499612.532490319365976No Hit
TGTAACCATGAGAAAGTGGGACTTCAAATACGGACCAATGTTAAGGATCT881456.695170544743593No Hit
GATTTTATGAGACAACAGAAGCATTATACTGCTTTTTTGATGCATAAAGC751175.705611501611034No Hit
CAGCTTCTGCTTTTGCTCACCACTAGGGTCACTGACCCTGTGGGGAAAAT525553.9918848259005006No Hit
GCTATGCATCTACCACCCTCACCCCTAAATCCTCCTGACTGCTGGCCTCA499363.792955202476784No Hit
TGTTAAGTCTTAGTCATTAGGGAGATACATATGGATACACTCACAAATTC480883.652587908056384No Hit
GAAGAAACCAGTGAAGAATCCCTCCTGACTCCCACATCTCCTTACCCTCC436523.3156456363849043No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG374842.8471470043583738No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG113760.8640791890294757No Hit
GTGGTGGTATACGATATGGGTTTTGTAAAAGTCCATGTTTATTTGGAGTA72040.5471893879894816No Hit
TGTGCTCACTGTACTTGGAATGTTCTCATTTCCCATTTCTCTTTCAGGTG71530.5433156152538536No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA67630.5136926472755224No Hit
AGGGACCATGTATAATAGGTTGTTTAACAGAACCACTGTTATTTTTGCAT66330.5038183246160787No Hit
CCCGTTTTCCCAGATATTTTAACACTGAAGAAAAGTTACTTTTGACAAGT65560.4979696873485621No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA64480.4897664039084088No Hit
CCAACTTTGTCCTTAACTAGCTCTTTTGGGACAATTCTGAGGAAATGTTC61500.4671314181198378No Hit
TGCATTATACCCAGCAGTATCAGTAGTATGAGCAGCAGCTGGACTCTAGA59940.4552822309285053No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT59540.4522439778025226No Hit
ACGAAAAAGTAACCTTGATGAAGAGGTGAATGTAATTCCTCCACACACTC58100.44130626654898497No Hit
CCTGCCCTGGAGGAAGACAGGGGAAAAGGTACAGACCAAGTCTCTGCTAC56790.4313559875613917No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT56070.42588713193462285No Hit
GAAGAAACCAGTGAAGAATCCCTCCTGACTCCCACATCTCCTTACCCTAG55220.41943084404190967No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC55150.4188991497448627No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTACTTCCAGCCCATCTGAGACC53580.40697400622538066No Hit
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA52110.39580842598739435No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC51550.39155487161101854No Hit
CAGTTAGCAGTTATAAATAGCCTGGAAAAGGTAAGTTACAACCTCTCTGG49580.37659147496555384No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG49310.37454065410551546No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTAGTCG49230.373933003480319No Hit
CAGCTTCTGCTTTTGCTCACCGTAACAATATAAGACACCAACATTAGGAA49020.37233792058917803No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG48750.37028709972913976No Hit
TGTTTCAGGAAGGAATGTTCCCAATAGTAGACATAAAAGTCTTCGCACAG46810.3555515720681237No Hit
GCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATTGAACTT46710.35479200878662803No Hit
CGACACATGACTGCAATGAAATTCAGTCCTGGAAGGAAAAGAAGAAGTAA45830.34810785190946614No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCCTGAAAACCAGAT44390.3371701406559285No Hit
CTCAGTCACACATGCCAGAGATTGCTCAGGAAGCAATGGAGGTAAGGGGA43490.33033407112246743No Hit
ACCTCCATCCAAAACTTCTCGAAAAGAAACTACCTCAGGGACAAGTACTG40940.3109652074443278No Hit
CCTATCTCATCGTCTTATATTGGCTTCACTGTCTTTCAACAGGCCTTGCT40690.30906629924058865No Hit
CAGTTAGCAGTTATAAATAGCCTGGGTTTTGATGTGTAACTTGTCAAGAC39440.29957175822189275No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA39330.2987362386122475No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT38840.29501437853291873No Hit
CCTCTCTCCTTCTTCCCCAAGTACACACACAAACCTGCCAATGCCAGTGG38220.29030508618764556No Hit
ATTTCTCTTCTGACCTCTCAATTTTACCTTTTCTAGATGTATTCTTGGGG37970.2884061779839064No Hit
TTGTGGCACCAAATACGAAACACCCATAAAGAAAAAAGAACTGAATTCTC37650.2859755754831202No Hit
TTACCCAGTACCATCAATGCTGTTAACAGTTCTTCATCCTTTTTCCAGCT37000.28103841415339836No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA36210.27503786422958254No Hit
GGTCCAGCAGCCCTTTCCTGAGCAAGCGGTCTCTGAGTCGGAGTCCACTC35900.27268321805694595No Hit
GGGATCGATGAAGGACCGGATATCGGGAAGTTCAAAGCGTTCGAATGAGG35640.27070835352505723No Hit
GAAGAGACCAAGCAAGTTTTGAGACTCAGTCTGACAGAGTTCTCATTACC34940.26539141055458754No Hit
GTCCTCCAGAGCACAAACCTGTGGCAGATACACACTGGTCCAGCCTTAAC34860.264783759929391No Hit
GAAGAAACCAGTGAAGAATCCCTCAAATGGCACAGCTGAGACCAAGTCTC34840.26463184727309186No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC34810.26440397828864315No Hit
AATTTGCATCTGTTTGTCCACATTAGGCTTAGGTTACCACAAGGATCTCC32990.25057992656542194No Hit
TCCCTTAGAGCTTCCACACATGGACTGGTCATTAATATCATTCACTCTCT32320.24549085257940095No Hit
TTAACTACAATTCCCAGCAAGCCGCGGAGCCAACCTGGGCCTCTGCCGGC32110.24389576968826004No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG32110.24389576968826004No Hit
ATATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTG32010.2431362064067644No Hit
GCCCTCTCAGAAACATCTGCAGGAGGACTTGGGGAAACAAATTCATATGA31980.24290833742231566No Hit
CTGTGGTGCCACCTAAGCTCTTAAGTGATAATTTATGTAGACAAACTGAA31890.24222473046896956No Hit
GCCAAACGAAAATTATGGCAGGTTGTTACGAGGCATTGGATGATTCAGAG31830.24176899250007217No Hit
CCAGTGCAACAGGTGGCTTGGGATCAATAAAAGCTGAGGTGATGGCAGAT31320.23789521976444422No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACACTCGGTAGCA31140.23652800585775205No Hit
TGTGATTATTTCTATTTTAGCAACCAAAGGACACAATGAGATTAGATGAA30980.23531270460735892No Hit
CTAACAGTACTCGGCCTGCTCGCTGGTATACCAAACTTGGATTCTTTCCT30890.23462909765401282No Hit
ACGCCTTCTGTCTGAGCACCCAGAATTAGAACATATCATCTGGACCCTTT30470.23143893187173104No Hit
TTAAATTTCACTCCACTTACCTACCAAGGGCCTGGTTTAAGGCCCTGTAT29950.22748920280795354No Hit
AGAGGAACGTTACACACCAGCCATAGATGTTTGGAGCTGTGGGTAAGGTT29800.22634985788571005No Hit
CAGCAGTTACCAGGGCACAGGGTCAGTGCAGTTTCCAGGGGACCAGGACC29550.22445094968197085No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT29500.22407116804122304No Hit
CCAGTGTTGTGTTTCCCAAAGTCTTTCCTGTTGTGTAAGTATCTCCTTTT28450.21609575358551847No Hit
TGACACACTTGCAGAAACAGTATTGGCTGATCGGTTTGAGAGATTGGTGG27710.2104749853024505No Hit
ACAATAGTGGCCAAAAATGCTCATTGATAATAACAGTTTACATTTGTTTT27320.2075126885046174No Hit
TTAGTTGCTTTTGAATTTACAGTTTAGTGAATTAATAATCCTTTTGTTTT27230.20682908155127128No Hit
TTTTGTGTTTGCACAGTGCCTTTCAGAATGTCCCACCCATAGAGTAGCAG26910.20439847905048514No Hit
GGAAATTGAAGAAATCTGCCTAGTAGATGAGAACCAGTTCACCTTAACCA26180.19885366709556673No Hit
AGTCTTCAGAATGCCAGAGATATACAGGATATGCGAATTAAGAAGAAACA26150.19862579811111805No Hit
CCACTGTAAGTTTATGTCATGGTTGTTTTTTATATTTCAGTCGCCCTTAC26110.19832197279851976No Hit
CCAGACTCTCCAGAACCAAAGGCAATCACACCACCTCAGCAACCATATAA26110.19832197279851976No Hit
TGCATTGAACAGATGAAACTAAAGAACTTTTTTTTTCTTAAGGTTGTAAG25750.19558754498513534No Hit
TGTGCTCACTGTACTTGGAATGTACACTGACGACATGGTTCTACATGGGT25540.19399246209399446No Hit
ATTACAACCCATCTGAGTGGAGCCCTGGCTTCTCAGGCAGACCTGGTGTC25510.19376459310954575No Hit
CCATCATGTGAGTCATCAGAACCTAACAGTTCATCACTTCTGGAAAACCA25040.1901946456865161No Hit
TCAATGGCAAGTAAGTTATTTATGGTCGTTTTTAAGGATAAGCTGTTAAC24820.1885236064672256No Hit
CTCACGTAATTTTGTACTTTTTCTTCCTATTGGTCTTTGTTTTTCTCTAG24460.18578917865384117No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT24050.18267496919970894No Hit
GGATACAGTAGCCAGAATGACGACTCAATCAGTGCTTCGGTTGTTGCTTT23970.1820673185745124No Hit
GTGCAGGCTGATTTTCTTTTTCCTGTGTATCTTCTACCAGGTGCTTGGGC23290.17690228826034185No Hit
TTTGCTAATAGATGCCTAAGCCCAGAAAGGGTGCTTCTTCAACTAAAATA23180.1760667686506966No Hit
TTTTTGTCCAGCCAGCAAATGAGAGTCTGTACCCGACCATTTCACAAAAG23100.17545911802550007No Hit
ATTTGTTGCAGGTTTTGGTTTTAATGGCTTGTGGCGGTTTGCAGGACCGT22870.17371212247806No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCAGAGTATTGCCAATCATGTTC22860.17363616614991045No Hit
TCCCCTTTGCTTTGTCTTTTTCCAGCCTCCCCCACTGGCAGGATTGCCAT22730.1726487338839661No Hit
TTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTA22690.1723449085713678No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG22480.1707498256802269No Hit
CTAATGAGCGGACGGCAAAGAACGCTTTTTCAGACGTGGGGCTCAAGTAT22410.17021813138317993No Hit
AGGTTCAACTACGCGTGTAAATTCTACTGCAAATGCAGAGACACAAGCAA22380.16999026239873122No Hit
CAGCTTCTGCTTTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTA22120.16801539786684247No Hit
GGCAGTTCTCTTGAGTCAGCTGATGAAAACCCAAGAGCCAGCAGGCAGTC21850.16596457700680417No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC21820.16573670802235546No Hit
ATCTGGTGTTGAAAATTCTAATGACTTTGCATTTTTGAAGGTTTTTCCTT21700.16482523208456065No Hit
ATACCACTGACGGCCAGGTAGTGATATAAAGGTAAGTAAAATGTTAGACC21560.1637618434904667No Hit
GACCTTTTCCTCTGCCCTTATCATCGCTTTTCAGTGATGGAGGAAATGTT21500.16330610552156932No Hit
TGTCATAATGAATGCCCCATCGATTGGTCAGGTAGACAGCAGCAAGCAAT21330.16201484794302667No Hit
CTACTCTTTAGCTTCCTACCTAAGAATAAAAATGGGATTGTTTGCACTAA21310.16186293528672754No Hit
TCAACTTGAGGGAGGGAGCTTTACCTTTCTGTCCTGGGATTCTCTTGCTC21030.15973615809853967No Hit
GCTGCCCCAAAGTGTAAAGAAATGCAGAATTCTCTCAATAATGATAAAAA20890.15867276950444573No Hit
GTAACTTGATTTGCTGTTGTATTTGCTTAGGAAGAGCAGCACTTCAGAAA20860.15844490051999702No Hit
TCTGGGGTATCAGGTAGGTGTCCAGCTCCTGGCACTGGTAGAGTGCTACA20730.15745746825405266No Hit
TTCAACGTTGCCTCCTTTGCCCCTCCCACCCTTATTACCTGGAGATGATG20210.15350773919027516No Hit
TGAACTTATATATTTGACACAACCCAGCAGTTCGTAAGTAGTTCACAGAA20170.1532039138776769No Hit
TTCCCAATCACAGCCATTCGTGAAATCAAAATCCTTCGTCAGTTAATCCA19960.151608830986536No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA19950.1515328746583864No Hit
GAATCAGGGATCACAACACCCCCCAAAATGAGGAGAGTAGCAGAAACTGA19760.15008970442354463No Hit
CCAGAGACCAGACTCCAGAGCTGGGAGGCGAGGTCACATTCCACTGGCCC19750.15001374809539506No Hit
AAAGCAGAAGCCTTAGAAACTCTGCAAATTATCAGAAGAGAATGTCTCAC19710.1497099227827968No Hit
TTTGCAGACCTTCTCAGGAGTCACTACTTCATTCCTGCTCTTAGTGTCTG19600.14887440317315156No Hit
AAACGTGATGAGACCCGAGTTATGATCTTCTCTTCATTTCGAGATAGTGT19440.1476591019227585No Hit
GTTAGAAGGCTGGCTCCCATGCTGTTCTAACACAGCTTCTAGTTCAGCCA19090.14500063043752365No Hit
TATGTCATGATTCATCTTACTAGCCTCAAACATATCTTCTTTGCCAGGAC19030.14454489246862626No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC18860.1432536348900836No Hit
GCATATGGAGAAACTTGAGGAAGACAGGGGAAAAGGTACAGACCAAGTCT18850.14317767856193403No Hit
GGTCTCTCATGGCACTGTACTCTTCTTGTCCAGCTGTATCCAGTATGTCC18470.1402913380922505No Hit
TGAAGAAGGAGATGGTGTGGAATTGATGGAAGCCAAATCACAGTTATTTC18420.13991155645150263No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC18100.1374809539507165No Hit
CTTAAATGTTGAAGGTGGTTCTTCAGAAAATAATCACTCTATTAAAGTTT18060.13717712863811823No Hit
AAGTCCTCCATTTCTGTATCCATGCGTTTAGGACTCAGTTCCTCTGGAAA17880.135809914731426No Hit
GGCATCTGTATATTTATTTTAAACACTGCTAATAATCTTTGTCTTTTTTG17540.13322739957434074No Hit
TATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTGA17090.1298093648076102No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG16840.12791045660387104No Hit
CCAGCTCACAAGAGAAGAAAATACTGCTATACGTACTCCAGAACATTTAA16630.1263153737127301No Hit
TGTTCATTCATGATCCCACTGCGATGGCCTCATCTTTGGGTTTTGATGTG16590.12601154840013187No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT16320.12396072754009356No Hit
GGTGGTGTACCTGGTGAAAGACTAATGAATTCATCGGACATGTTACTGTT16300.12380881488379442No Hit
TTTTGTGCGAAGGAAACCGATGGGGATCGGAACCGTAGCGGTTGAGCTGC15940.12107438707041No Hit
GCCTACCAGTCCAGCACCCGGTCACCGAGCCCCTACAGTAGGCGACAGAG15850.12039078011706389No Hit
TATTTCTGGACAGTCTACGAAAAGCTCTTGCTGGCCATGGAGGAAGTAGG15830.12023886746076475No Hit
CGATGCGCGGGAAGACGTTCCGCTTTGAAATGCAGCGGGATTTGGTGAGT15480.11758039597552991No Hit
GGGATAATGGGGTTTTCCTGTGTCAGAATCTCAATGGAACCCAGCATCCT15360.1166689200377351No Hit
TGTTAAGTCTTAGTCATTAGGGAGATACATATGAATACACTCACAAATTC15000.11393449222435069No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT14790.11233940933320977No Hit
TCAAAGGGCTCCACTGGTTTTTCTGAGCAGGACTTCACTTTTTCAAGCTT14690.1115798460517141No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA14580.11074432644206887No Hit
GTAACCAGGCATTATGATCTTTGAAAATTTTTATTTGCGTATCTTTAATT14510.1102126321450219No Hit
AACTGAGCGACCTCAGAGCCCTGGCAGCTCCAAGGCGCCTTTGCCAGCAG14450.10975689417612448No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTAGTCGTAACACATC14370.10914924355092796No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA14270.10838968026943228No Hit
GCCACAAAGTAAAAATGTTGTGTGTTTACTTTTTTGCATCTTGGCAGGCT14140.10740224800348792No Hit
GGAAACACTGATGGGCCTGAAACAGGGTTCAGTGCCATTGACACTGATGA13880.10542738347159918No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT13810.1048956891745522No Hit
CCAAGGAACATCTTCAGTATCTCTAGGATTCTCTGAGCATGGCAGTTTCT13760.10451590753380437No Hit
CAACTTGTATTCAGCAGGTACCGCACTTCTTGAACAAAACCTGCATACTT13690.1039842132367574No Hit
GCAGGAAGGAAAAGGAACCTGTATTCTTGTAGGTGGTCATGAAATCACTT13650.10368038792415912No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG13550.10292082464266344No Hit
CACAAAGGTTTTTATAAGTTCTGTGGATCTTTTAATTGCAGATTTGCATT13520.10269295565821475No Hit
ATATATGTCTTCCACCCTTGACTCTCAGGATAGTGCAGCAGGATGCAGCG13380.10162956706412081No Hit
TCAATGCAGAGGTTGAAGATGGTATGTTGCCAACACGAGCTGACTCTGGG13190.10018639682927903No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)