FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316546
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACATTTCCTCAGAATTGTCCCAAAAGAGCTAGTTAAGGACAAAGTTG15735211.951880146990685No Hit
CTGGAGTGTGTGGAGGAATTACATTCACCTCTTCATCAAGGTTACTTTTT788505.989156474593368No Hit
AGAAAGGTGAAAAATAAGAGAACACTTTACCTGTCCTTGGGAGTCAAAAA763255.797366746015711No Hit
AGAGTCCAGCTGCTGCTCATACTACTGATACTGCTGGGTATAATGCAATG465583.5363747259875464No Hit
TGACAAGTTACACATCAAAACCCATCTTGATGAAGAAACTGGAGAAAAGA459433.489661584175562No Hit
GTACCTTTTCCCCTGTCTTCCTCCTAACCTCTTGTGTTGATTTTCTTCTC457133.4721916287011623No Hit
GGAATTCCTAATGTTGGTGTCTTATATTGTTGCTCAAAGTCATATAAAGA431943.280857638092402No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT426783.241664172767226No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC116730.886638218489897No Hit
CAGTTCAGAGGCAACGAAACTGGACTCATTACTCCAAATAAACATGGACT101300.769437604155115No Hit
AACATTTTACTTACCTTTATATCACTACCTGGTGTGGCACTTAGAGCCAA91010.6912785424892104No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG65520.4976658620359638No Hit
ACCCAAGAACATTTTCCCCACAGGGTCAGTGACCCTAGTGGTGAGCAAAA63900.4853609368757339No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT63740.48414563562534085No Hit
CAAAATCTAAAGCAGCATACCTTTGTAGAACTTCATCACAGGTGAGTTCC63010.47860082367042245No Hit
AGAGTCCAGCTGCTGCTCATACTACTGATACTGCTGGGTATAATGCATGT59490.45186419616177487No Hit
CCAGATCCTTAACATTGGTCCGTATTTGAAGTCCCACTTTCTCATGGTTA56740.43097620592064384No Hit
GTACCTTTTCCCCTGTCTTCCTCCAGGGCAGGTGTAGAACCATGTCGTCA56380.4282417781072595No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT55870.4243680053716315No Hit
AGGGTAAGGAGATGTGGGAGTCAGGAGGGATTCTTCACTGGTTTCTTCTG54630.41494942068108526No Hit
CAGATGGGCTGGAAGTAAGGAAAGAAATATCTGAAATATGCAGCATGTAG53150.40370788411494923No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT52080.3955805570029456No Hit
CGAACATTTCCTCAGAATTGTCCCAAAAGAGCTAGTTAAGGACAAAGTTG51980.3948209937214499No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG51170.388668531141335No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG50830.3860860159842497No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT49890.3789461211381904No Hit
GTAGTCAGTGCATTCTACAACAGCGCGCATGTTAGCAAGTTCATCAACCA49650.37712316926260075No Hit
CCAGAGAGGTTGTAACTTACCTTTTCCAGGCTATTTATAACTGCTAACTG49270.3742368287929172No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACCTCCATGATCTCCAACAAAG48590.36907179847874666No Hit
GGAATTCCTAATGTTGGTGTCTTATATTGTTACGGTGAGCAAAAGCAGAA48590.36907179847874666No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC44750.3399045684693129No Hit
GCTGTCTACCTGACCAATCGATGGGGCATTCATTATGACATGAAGATCAG43960.3339040185454971No Hit
CAACTGTCAGTCTGCCATTCTTTAAGACAGCTAAGAGGGGAGGATCTAAC43920.3336001932328988No Hit
AGCTTCATCATACCTTTCACTAAGACAAGAATTTAGAAGTGGACAGGAAA42800.3250930844801473No Hit
GCACAAAGGCCCCATTCTGGCACTGACCTATTGCATCCCCAGCCCCAGAC42040.3193204035407802No Hit
CAGCTGTGCCATTTGAGGGATGCTCAGGTCGGTTTGGCTCTGCAGCAGGT41630.3162061940866479No Hit
CAATGATGAACACTGCGGGCTTCCAGACAATGTTAACACGCTGAATGCCA41390.3143832422110583No Hit
TGACAAGTTACACATCAAAACCCAGGCTATTTATAACTGCTAACTGTGTA40380.30671165306795206No Hit
CAGAAATGACATCTACCTGTTACTCACAGAAACCACACACCAAAGGAACA39580.3006351468159867No Hit
GGATCCGTGGCGAGTTCGTCCACTGTTACAGTGGCCAGTGGTAGGGGAGA39100.29698924306480745No Hit
GCAGTCTGGGGCCCTTTGGCAGGCCCTGGTTGCCCTGAGGCGGGTGCTGC38170.28992530454689774No Hit
CGTCAGCTATTGAATTACTTTCCAAAAGAGAAATTTCATTGAATTTTTTA38000.28863404696835504No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC37420.2842285799356802No Hit
CACTCAAGAGCACATGTACAGCTGTACCTGATCAGTTCTAGCTGAGCCAG37120.28194989009119314No Hit
GTGTACACAGTGTCCACCAAGGTCCTGAGATCCTCATTTCTCTTCCTTGT36730.27898759329336004No Hit
CGTATACCACCACTTTTTCCCATCAAGTCATTTGTTAAAACTAAATGTAA36410.2765569907925739No Hit
TCCACCAATCTCTCAAACCGATCAGCCAATACTGTTTCTGCAAGTGTGTC34930.265315454226438No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA34740.2638722839915962No Hit
CAGCTGTGCCATTTGAGGGATTCTTCACTGGTTTCTTCTGTAGAACCATG34600.26280889539750224No Hit
GGTCTTTGTGCCCAGACTTCAGCTCCCGTTCTTTCCTGGTCTTCTCCTTG34580.26265698274120314No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA34550.26242911375675443No Hit
TAGGAGCCAGGAGAGAATGACCTGTCCCGGTAACTGGAACGGAAGGCAAT33900.25749195242703254No Hit
TAATCTATATTGATCAGGTGAAGTAAAATGGAGAAAGGAACTGGTAAACA31720.24093347289042694No Hit
TGCAATTTGGTCTCTGCATGTACCACAGAATTGCTGCTTCCCTCAGCCTT30860.23440122866956414No Hit
TGTGGTTTAGCCCTCGAAGTAAGAAAGTCAGATATGTTGTGAGTAAAGCT30630.2326542331221241No Hit
TGGTGGCAAACTCTCCTTCTCAACCAAAAAGAAAGCAATCAACTATTTCA30570.2321984951532267No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT30330.2303755432776371No Hit
ACTTTCAAGTTTCCCCACCCTTTACACAGTTTCCCCGCGGAAAACGCCGC30290.2300717179650388No Hit
GCAGAATTCCGAAGTTCAGCTGCATGCTGGTTTCCTTCACGACAGGTGTG29830.22657772687015876No Hit
AGTGAGGATGAAGAGCTTCCCTGCTTCCAACACTTGTTATTTGGTAAAGT29720.22574220726051347No Hit
GTTGTAAAATTTCTTCACTCTGAATGTCAGCAAAAACCTTATGTGAATGC29710.22566625093236392No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTGCTTGATTTTCTTACTTGGTCCAA29700.22559029460421437No Hit
GGTTGCTGAGGTGGTGTGATTGCCTTTGGTTCTGGAGAGTCTGGGGGAGA29500.22407116804122304No Hit
CGAAAGGTGAAAAATAAGAGAACACTTTACCTGTCCTTGGGAGTCAAAAA29410.2233875610878769No Hit
ACCTGTATAGGGTATGCTCTTTGAATAATTACATCAACACAACCAACATT29390.22323564843157775No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC29100.22103291491524032No Hit
CTGTGACAGTTCCCAGCGGGTCCGGATATGAATGATAGACTGGACAATGG28910.21958974468039857No Hit
ACAAGTTGCAGGACTTTTTGCTGTTTCTTTTTCTACATTTTCATGTACTT28890.21943783202409942No Hit
GGTTGAACTAAAACGACAATACAATGACCACCATTCAAAATTGAGAGGTC28890.21943783202409942No Hit
TTATCGCCATCCTTCTGAACTTAAGAAGAGCTCTAAAAAGCCAGTGTACA27730.21062689795874964No Hit
ATCACAATATCTCATTTTGCCCTCTTTGCAACCAGTGCACATTACTGGGT26930.2045503917067843No Hit
GGCACTTTTCTTCCCCGCTTACTCTAATCACTTACCCACTGAGAACAAGG26920.2044744353786347No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTACAT25970.19725858420442582No Hit
TTATTTTTTGGGGGGAAATTTTTTAGGATCTGATTCTTCTGAAGATACCG25970.19725858420442582No Hit
TACTGGTGTGGGTTATAGAAGAAACATGAGAGATTTAGTTCAAAAGGAAG25840.19627115193848144No Hit
TATGAGAACACGCAGAGGGAACTTGGCCTCCTACTGCTGCTTTCAGAGAG25570.19422033107844314No Hit
CACAACCTTGCACTGCTTTATGAAGTGTTTCACCAAACATATTTCGAAGT25260.19186568490580655No Hit
AAAGAGAGTGAGTCGTTGCGAAGCCCTGTGTTTCAGCTCATTGTGATTAA25230.19163781592135787No Hit
AAAAGCCCCTAAACCCCACTTCATTTTCATCTGTTAAATAACCAGAAGCA24690.18753617420128124No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGAATAAGCAGAAACTGCCATGCTC24640.1871563925605334No Hit
GGAAGGTGACGTGAAAGATCACTGTGCAGCAGCAATCTTGACTTCTGGAA24570.18662469826348643No Hit
CCTCAGCTTGCCATCAGGAGTCGTTACCTTTGCTAAGCTCGGAGGGTTTC24420.18548535334124291No Hit
AGCCTCATTATATGTCCTCTTACTCTCTCACCTCAAGGTAAGCTGGGTCT23870.18130775529301674No Hit
ATTGTGTGTTCTTAAAGCAGGCATACTAATTTGAACAGAAACCTTCATTT23790.1807001046678202No Hit
AGGGCCTATTAGCCATATCTCCCAGCTCTCAACAGGAGGTGGAGAGGCAG23500.17849737115148273No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT23340.17728206990108966No Hit
CATGAGGGTAGTGTTGACAAAGATTAAAACAGAAAATGCTTTATGTTTAG23250.17659846294774356No Hit
GTGGATTAACTGACGAAGGATTTTGATTTCACGAATGGCTGTGATTGGGA22980.17454764208770526No Hit
GTCACTGACAACTGGCTTGTGCAACATTTTGACATGGAAGTCACAGACTA22370.16991430607058167No Hit
TTACCCACAGCTCCAAACATCTATGGCTGGTGTGTAACGTTCCTCTCCTA22360.1698383497424321No Hit
AGGAAATAATGAGCGAACCCCACTTCCCCACAACGGCACCCCACATGAAA22310.16945856810168425No Hit
CGACAAGCAACACATCATCGTCCGACTCCAGCTGAGCCTCCGCTGCTGCT22300.1693826117735347No Hit
ATTAGGATGTCTGGCACATGCACAGGACAACCAAGTTCAAGAACCTCTCA22210.16869900482018857No Hit
GTAGACGATGTAAAGCAAGCACATTGCCGTCACTTATGAAGGAAAGACTA22110.16793944153869292No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC22040.16740774724164595No Hit
GAGTCCTCCTTCTGTGAGCAAAAGCAGAAGCTGTGTAGAACCATGTCGTC22020.1672558345853468No Hit
CAGTACTTGTCCCTGAGGTAGTTTCTTTTCGAGAAGTTTTGGATGGAGGT22020.1672558345853468No Hit
CATTCCTTGTTGTGCTCAGTACTGACTTTGGTATGTGTGTTCTGCTGTCC21820.16573670802235546No Hit
GATGTTTTGAGATTTTCAGTTTGTCTACATAAATTATCACTTAAGAGCTT21740.16512905739715894No Hit
GTCCATGCTCTTGAAAATAATCATCTACTAACCTTTTTTATAAAACAGTG21620.16421758145936413No Hit
GCACCTGAGCTCAATGAGATTCATGACCGGTCTGTTTACCTGCTGCCTCC21620.16421758145936413No Hit
AACATTTTACTTACCTTTATATCACTACCTGGCCGTCAGTGGTATTGTAG21350.16216676059932583No Hit
AGAAAGAAATATATGGTAAGTTTCAAGAATACATCATACCTGTATAGGGT21230.16125528466153102No Hit
CTGAGTAAGTGGCAAGAAAATTACCTGCGTGCTGCATCAAAGTTGCTTTT20950.15912850747334312No Hit
AACATGTTCCAGAGCAGTTGGTAGAGTAAATGCCGAGAATCAAACAGAGT20380.15479899676881778No Hit
TATGAAGTAGCCTGCTATAATCGATCAAACTAACCCTAACTATCAAGATG20240.15373560817472387No Hit
AAGAGGCTTCCAAATTGAGGGTGGGCGCCTGGGAGGGGTGTTTCTGTTGC20240.15373560817472387No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG20170.1532039138776769No Hit
CCTGGAGTCGATTGATTAGAGCCTAGTCCAGGAGAATGAATTGACACTAA19940.15145691833023683No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT19880.15100118036133944No Hit
ATATGACGTGTCTGCTCCACTTCCATTGAAGGAAGCTTCTCTTTCTCTTA19640.14917822848574983No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCTGGAAAATAAACATGAC19320.1467476259849637No Hit
ATTGGACCAGTCTACCCTGTAAAAATGTGATTCTTTGCTAGTACCTTTGT19280.14644380067236543No Hit
AATTACCTCCAGTTGCTCCTTCTGGGTAAAACCCTTCGTGCTTTTCCCTG19070.1448487177812245No Hit
GACAGGTTCATGAGTGCTTCCCCCACATTCCTTCAGGTGACTGGTTCTTC19040.1446208487967758No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG19020.14446893614047668No Hit
CACTCCACCCCCAAGAATATCATCTAGTGCTGAACAGAAGGTGATTATGA18860.1432536348900836No Hit
CCAGTCCTCAGATAAATGGTGAAATAGACTTACTAGCAAGTGGCTCCTTG18680.14188642098339138No Hit
GTACCTTTTCCCCTGTCTTCCTCAAGTTTCTCCATATGCTGTAGAACCAT18630.14150663934264354No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACAATATCACC18500.14051920707669918No Hit
CTGCCCCCTTACCCTCCACACCCCCAGGATTCTTACAGAAAACAAGTGGT17740.13474652613733207No Hit
TCTAAAGTATGCAGGTTTTGTTCAAGAAGTGCGGTACCTGCTGAATACAA17550.1333033559024903No Hit
AACAGATATGCTATAGTACAGAAGGCAAAAGAAAAGTGATTACCTTGCTT17480.13277166160544335No Hit
CACGCACACACAAGCAGATTGCTCAGATTACTAGAGAGCTCCGGAGGACG17380.13201209832394764No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTCTTGGTGCCATTTATCGTTTTTG17360.13186018566764854No Hit
ATGTTACCTTAAGATCAACCACCATGGACTGAACAAGTAGGAAAATGACA17340.1317082730113494No Hit
ACCTGATGAAGACTTTGGACCTCTTAAGCTTGAAAAAGTGAAGTCCTGCT17130.13011319012020847No Hit
TGGCTTTGCATAATATGGCTTTAAAGTCAGTAAGACATGGTATATCAGCA16910.128442150900918No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG16780.12745471863497365No Hit
CGAGTCCAGCTGCTGCTCATACTACTGATACTGCTGGGTATAATGCAATG16390.12449242183714053No Hit
TGACAAGTTACACATCAAAACCCAAAGATGAGGCCATCGCAGTGGGATCA16250.12342903324304658No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA16240.123353076914897No Hit
GAAAACTTTTATTGATTTATTTTTTGGGGGGAAATTTTTTAGGATCTGAT16010.12160608136745697No Hit
CCTGTCATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTA15980.12137821238300828No Hit
ACGTTTTTAGGTGAAGCCTGTTCTTTTCCCAAAACATGAATGTTCTCAAC15710.11932739152296995No Hit
CGACTCAGAGACCGCTTGCTCAGGAAAGGGCTGCTGGACCGCCTTCGACC15650.11887165355407255No Hit
CTGAGGTCGCTCAGTTCCGGAGCTGCAACCCGGAGTCCCAGATGATCGGG15410.11704870167848294No Hit
AGAACATTTCCTCAGAATTGTCCCAAAAGAGCTAGTTAAGGACAAAATTG15290.11613722574068813No Hit
GTCTCTGTATGGCAGCAAATGTGGCCCATGGCCTGCAGCAAAACGTCCTA14890.11309897261470546No Hit
TCAAAAGGAGATACTTACACAACAGGAAAGACTTTGGGAAACACAACACT14840.1127191909739576No Hit
CGACAAGTTACACATCAAAACCCATCTTGATGAAGAAACTGGAGAAAAGA14820.11256727831765849No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT14810.11249132198950891No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC14680.11150388972356454No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT14540.1104405011294706No Hit
TCTGAGCACCAGTGGAGATTTTGTTAAGCAAACGGTGCCCTCAGAAAATA14520.11028858847317147No Hit
ACTTGCTTTCCACTTGCTGTACTAAATCCAGAGAAAGCAGATGAATTTAC14400.10937711253537666No Hit
AGTGACGTGTCCTCTGTTCCTTCTCTTTCTTCACAGGTTTTGAAGGAAGA14380.10922519987907751No Hit
CACTGGGCAATTGGTAGGGTAAATCCACAGGGCGCCCGCGGAGGTGCAGA14360.1090732872227784No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT14060.10679459737829138No Hit
CTACCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGATCCCCCACA13420.10193339237671908No Hit
TGACAAGTTACACATCAAAACCCATATCGTATACCACCACTGTAGAACCA13320.10117382909522342No Hit
AAGTGAGGGCGGAACAGGTACCTTGCTAAGAATACGGATTATCTGCTTTA13300.10102191643892428No Hit
TCCCCTGGAAACTGCACTGACCCTGTGCCCTGGTAACTGCTGGACTCCCC13220.10041426581372773No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)