FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232543
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATTGAACTT966127.838428355035078No Hit
TCCCTTAGAGCTTCCACACATGGACTGGTCATTAATATCATTCACTCTCT953457.735632752772115No Hit
TGAACTTATATATTTGACACAACCCAGCAGTTCGTAAGTAGTTCACAGAA747836.067374525675778No Hit
AGCAAGCTGTGACGGGTAAGCTTTATAAGGAAAGTAAATCTTCACCCCAC706655.7332685350531385No Hit
ACAATAGTGGCCAAAAATGCTCATTGATAATAACAGTTTACATTTGTTTT649095.266266572444126No Hit
CCAAGGAACATCTTCAGTATCTCTAGGATTCTCTGAGCATGGCAGTTTCT622025.046639346456878No Hit
GGGATAAAAGCACAAAGGTCTGCTGATTTCTCACCAGCATTCTTATCTAC490483.979414916964357No Hit
GGCAGTTCTCTTGAGTCAGCTGATGAAAACCCAAGAGCCAGCAGGCAGTC489783.973735601922205No Hit
AAGAAAGGAAATACTTAAGACACCAGATTCTAGTAAGGAAAAAGTAAACC441103.5787798072765007No Hit
ATCATGATAAAACTCTTCAGACTGATTCTATAGACAGGTATTGCACATGG422503.4278722932993007No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATGTAAGTCCAAAAGG317492.575893903904367No Hit
GCTATTCCTACCATTCTGATGAGGTATATAATGATTCAGGATATCTCTCA301352.444945125646732No Hit
TTCAGGTGTATTTTGGAGGCCACTGCTATGGCTTATGAAAATAATTGTTT215351.747200706182259No Hit
TGAACTTATATATTTGACACAACCCAGCAACCCATTCAGCAGAATTAGAC184621.497878775831756No Hit
GGCAGTTCTCTTGAGTCAGCTGATGAAAACCCAAGAGCCAGCAGGTAACC161961.3140312346100702No Hit
GGGATAAAAGCACAAAGGTCTGCTACCGTAACCTTCAGAGCTAGGGAAAA118250.9593985767636504No Hit
GGGATAAAAGCACAAAGGTCTGCTACCGTAGCTTAGTGACCTTTAGATAT38490.3122811942463671No Hit
GGGATAAAAGCACAAAGGTCTGCTACCGTACCTTCAGAGCTAGGGAAAAA30980.2513502571512718No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA27740.22506314181330792No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA27700.2247386095251849No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT26970.21881589526693998No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC26690.2165441692500789No Hit
TTCCCAATCACAGCCATTCGTGAAATCAAAATCCTTCGTCAGTTAATCCA26270.2131365802247873No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG25990.2108648542079262No Hit
AAAAGCAGTGGTCCAATGTATCTGCATAAATCCTGTCATTCTGTTGAAGG25350.20567233759795803No Hit
TTGCACAAGGTTTGAATCAAGTAACAGGCAGGTCCTTCAGACCAAGTCTC24750.20080435327611287No Hit
TCCAAAGTAATATCCAATGTAAAAGATGCAAATGCATACCCACAAACTGT23650.1918797153527301No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTACTTCCAGCCCATCTGAGACC23160.1879041948232232No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC22320.18108901677263997No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGCATTCAGATCTACCTTTTTTT22260.18060221834045545No Hit
GGGATAAAAGCACAAAGGTCTGCTACCGTAGGTTCTGAATTTAGATGTAT21790.1767889639550101No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGAGACCA21110.1712719150569189No Hit
GACTACAGATGTGCCACCATACCCACAAACTGTAAATGAAGACCAAGTCT20860.16924358825615007No Hit
GGGATAAAAGCACAAAGGTCTGCTACCCGTAGCTTAGTGACCTTTAGATA20630.16737752759944277No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG18660.1513943124093845No Hit
GCTATTCCTACCATTCTGATGAGGTTCTGAATTTAGATGTATGAAATAGG18560.15058298168907697No Hit
GCTATTCCTACCATTCTGATGAGGTAGACCAAGTCTCTGCTACCGTAGAG17970.14579613043926257No Hit
GTGGATCGGGGTTTAATTTTATGTTCTAGTTTTTACTTGAACAGAGGCGG17530.14222627526990944No Hit
GGGATAAAAGCACAAAGGTCTGCTACGTACCTTCAGAGCTAGGGAAAAAG17520.1421451421978787No Hit
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA17360.14084701304538663No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC17150.13914321853274086No Hit
GGGATAAAAGCACAAAGGTCTGCTACCGAGGTTCTGAATTTAGATGTATG17100.13873755317258707No Hit
CCAAATAACAAGTGTTGGAAGCAGGGAAGCTCTTCATCCTCACTAGATAA16840.1366280932997875No Hit
TGTTTCAGGAAGGAATGTTCCCAATAGTAGACATAAAAGTCTTCGCACAG16120.1307865121135733No Hit
ATTTCTCTTCTGACCTCTCAATTTTACCTTTTCTAGATGTATTCTTGGGG16000.1298129152492043No Hit
TTCAGGTGTATTTTGGAGGCCACTTCATTAGTACTGGAGACCAAGTCTCT15660.1270543908001587No Hit
TTGTGGCACCAAATACGAAACACCCATAAAGAAAAAAGAACTGAATTCTC15490.1256751285756359No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT15280.1239713340629901No Hit
GAGTTTGATGCACAACTTCAAGGCAATCTCATTGAGCTCAGGTGCAGACC15050.12210527340628279No Hit
TTACCCAGTACCATCAATGCTGTTAACAGTTCTTCATCCTTTTTCCAGCT14730.1195090151012987No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCCTGAAAACCAGAT14630.11869768438099117No Hit
ACGCCTTCTGTCTGAGCACCCAGAATTAGAACATATCATCTGGACCCTTT14420.11699388986834536No Hit
CCTATCTCATCGTCTTATATTGGCTTCACTGTCTTTCAACAGGCCTTGCT14250.11561462764382256No Hit
CGACACATGACTGCAATGAAATTCAGTCCTGGAAGGAAAAGAAGAAGTAA14150.11480329692351504No Hit
CTTTTTAGGAGAACCCTCAATCAAAAGAATGGCAGACTGACAGTTGAGAC14080.11423536541929978No Hit
AGACATCGCTGAATTTAAAGGATACAGATGAAATTGCTGCCACAGACCAA14070.11415423234726901No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG13990.113505167771023No Hit
GGGATAAAAGCACAAAGGTCTGCTACCGTACTCCATTTTACTTCACCTGA13860.1124504378346232No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT13810.11204477247446945No Hit
TTCAGGTGTATTTTGGAGCCCACTTCATTAGTACTGGAGACCAAGTCTCT13750.11155797404228493No Hit
TCAATGGCAAGTAAGTTATTTATGGTCGTTTTTAAGGATAAGCTGTTAAC13700.11115230868213118No Hit
TCTAAGCATAGCATTCAATTTTGGCCACTACAAATCATTCTCCTTAAGAC13360.10839378423308557No Hit
TGGGTCCAGTCAGTGAACGTAAGGGTTCTATGATTTCAGTGATGTCTTCA13270.10766358658480882No Hit
CCTCTCTCCTTCTTCCCCAAGTACACACACAAACCTGCCAATGCCAGTGG13250.1075013204407473No Hit
TTCCCCACTGGAGCTTATGAAGGTGACTGGTCTGAGTTATCGAGGTGTCC13120.10644659050434752No Hit
GGAAATTGAAGAAATCTGCCTAGTAGATGAGAACCAGTTCACCTTAACCA13050.10587865900013224No Hit
GCAATAAAACTAGTAGTGCAGATACCCAAACTCCATTTTACTTCACCTGA12960.10514846135185546No Hit
ATTACAACCCATCTGAGTGGAGCCCTGGCTTCTCAGGCAGACCTGGTGTC12740.10336353376717891No Hit
GGGATAAAAGCACAAAGGTCTGCTGCTTTAGATTTTGAGACCAAGTCTCT12610.10230880383077913No Hit
TTCAGGTGTATTTTGGAGGCCAAATCACAGTTATTTCTTAAGAGACCAAG12580.10206540461468686No Hit
AACTGAGCGACCTCAGAGCCCTGGCAGCTCCAAGGCGCCTTTGCCAGCAG12450.10101067467828707No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)