FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232543
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCTATATTGATCAGGTGAAGTAAAATGGAGAAAGGAACTGGTAAACA919237.457995380282878No Hit
CAACTGTCAGTCTGCCATTCTTTAAGACAGCTAAGAGGGGAGGATCTAAC913357.410289133928797No Hit
TATGAAGTAGCCTGCTATAATCGATCAAACTAACCCTAACTATCAAGATG721555.85415681237896No Hit
TTCTTTGTAAGGCGTGTCTCAATATTTGACATTTTCATTTCTGTTGTAGT684455.553153115144867No Hit
GCACCTGAGCTCAATGAGATTCATGACCGGTCTGTTTACCTGCTGCCTCC661325.365492319537736No Hit
GTGGATTAACTGACGAAGGATTTTGATTTCACGAATGGCTGTGATTGGGA648815.263994846427265No Hit
CAGATGGGCTGGAAGTAAGGAAACATGTAATGATAGGCGGACTCCCAGCA630375.114385461602557No Hit
ATCTTACCTTCAACAGAATGACAGGATTTATGCAGATACATTGGACCACT421753.4217873128969942No Hit
GTTATAAATGATATCTAAAGGTCACTAAGCTAAAGACTATATAATCTACC418113.3922548746778003No Hit
ACTTTTTCCCTAGCTCTGAAGGTTATAGGTCAATGGAAGAAGGTACGAAA317592.5767052346246744No Hit
TCATTTACAGTTTGTGGGTATGCATTTGCATCTTTTACATTGGATATTAC307712.4965457594582907No Hit
TCCTATTTCATACATCTAAATTCAGAACCTTTTTGAAAACCAAGAGTGCA292912.3764688128527767No Hit
AACAAACCTTATTTCCCACATGTAATTTTAATTAATGCCTTACCTCTCTT206781.677669663451904No Hit
AATTCTGCTGAATGGGTTGCTGGGTTGTGTCAAATATATAAGTTCATGTA182861.4835993551543434No Hit
ACTTTTTCCCTAGCTCTGAAGGTTACGGTAGCAGACCTTTGTGCTTTTAT107280.8703955967459147No Hit
GTTATAAATGATATCTAAAGGTCACTAAGCTACGGTAGCAGACCTTTGTG38580.31301139189464383No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGAATAAGCAGAAACTGCCATGCTC32970.2674957384853916No Hit
ACTTTTTCCCTAGCTCTGAAGGTACGGTAGCAGACCTTTGTGCTTTTATC28360.23009339227921458No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG28300.22960659384703008No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT26890.21816683069069395No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG26560.2154894393136791No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC25860.20981012427152643No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT25610.2077817974707576No Hit
GAAGGACCTGCCTGTTACTTGATTCAAACCTTGTGCAATGTAGAACCATG24720.20056095406002064No Hit
TTCAATGCTTCTTTGAATCTTTGTAGGTTTACTTTTTCCTTACTAGAATC24490.1986948934033133No Hit
GAAAGCACTATGTCTTCAATGTGGTCTGAATATACAATTGGTGGGGTGAA23920.1940703082975604No Hit
ACCCATCTGTAAGTTCAATAATGGCCACTTTTTGGGTATCTGCACTACTA23790.19301557836116062No Hit
GAAAAATAACATTCTGTGAACTACTTACGAACTGCTGGGTTGTGTCAAAT23690.1922042476408531No Hit
CAGATGGGCTGGAAGTAAGGAAAGAAATATCTGAAATATGCAGCATGTAG23320.18920232397571526No Hit
CCAAAGCAGAAAGGGTAGTAGATAAGAATGCTGGTGAGAAATCAGCAGAC22590.1832796097174703No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT21770.17662669781094859No Hit
CAACTGTCAGTCTGCCATTCTTTAAGACAGCTAAGAGGGGAGGATGTAGA21320.1729757095695647No Hit
TCCTATTTCATACATCTAAATTCAGAACCTACGGTAGCAGACCTTTGTGC21140.17151531427301117No Hit
ACAGAGAGTGAATGATATTAATGACCAGTCCATGTGTGGAAGCTCTAAGG20850.16916245518411932No Hit
GTTATAAATGATATCTAAAGGTCACTAAGCTACGGGTAGCAGACCTTTGT20760.16843225753584257No Hit
TCATTTACAGTTTGTGGGTATGGTGGCACATCTGTAGTCTGTAGAACCAT20760.16843225753584257No Hit
ATCTTACCTTCAACAGAATGACAGGAATAGCTGTAGAACCATGTCGTCAG19700.15983215190058278No Hit
ACTTTTTTTTTTTTTTTTAAGGTTATAGGTCAATGGAAGAAGGTACGAAA19640.15934535346839826No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG19580.15885855503621374No Hit
ACCTCATCAGAATGGTAGGAATAGCTGTAGAACCATGTCGTCAGTGTAGA18050.14644519501550857No Hit
TCCTATTTCATACATCTAAATTCAGAACCTCATCAGAATGGTAGGAATAG17970.14579613043926257No Hit
CAAATATACCATGTGCAATACCTGTCTATAGAATCAGTCTGAAGAGTTTT17540.1423074083419402No Hit
ACTTTTTTTTTTTTTTTGAAGGTTATAGGTCAATGGAAGAAGGTACGAAA17500.14198287605381718No Hit
AACAAACCTTATTTCCCACATGTAACCATTCCTGTCATGTCCGCCTCTGT17200.1395488838928946No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA17170.13930548467680234No Hit
AGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGG16670.13524883107526472No Hit
TCCTATTTCATACATCTAAATTCAGAACCTCGGTAGCAGACCTTTGTGCT16610.1347620326430802No Hit
GTAGTCAGTGCATTCTACAACAGCGCGCATGTTAGCAAGTTCATCAACCA16440.13338277041855742No Hit
CGTCAGCTATTGAATTACTTTCCAAAAGAGAAATTTCATTGAATTTTTTA16080.1304619798254503No Hit
ACTTTTTCCCTAGCTCTGAAGGTACGTAGCAGACCTTTGTGCTTTTATCC15950.1294072498890505No Hit
CCAGTACTAATGAAGTGGCCTCCAAAATACACCTGAATGTAGAACCATGT15660.1270543908001587No Hit
AGCTTCATCATACCTTTCACTAAGACAAGAATTTAGAAGTGGACAGGAAA15090.1224298056944058No Hit
GCACCTGAGCTCAATGAGATTGCCTTGAAGTTGTGCATCAAACTCTGTAG15010.12178074111815977No Hit
CACTCAAGAGCACATGTACAGCTGTACCTGATCAGTTCTAGCTGAGCCAG14770.1198335473894217No Hit
GGATCCGTGGCGAGTTCGTCCACTGTTACAGTGGCCAGTGGTAGGGGAGA14300.11602029300397633No Hit
ACTTTTTCCCTATCTCTGAAGGTTATAGGTCAATGGAAGAAGGTACGAAA14260.11569576071585332No Hit
GCTGTCTACCTGACCAATCGATGGGGCATTCATTATGACATGAAGATCAG14070.11415423234726901No Hit
CAACTGTCAGTCTGCCATTCTTTTGATTGAGGGTTCTCCTAAAAAGTGTA14060.11407309927523826No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTGCTTGATTTTCTTACTTGGTCCAA14060.11407309927523826No Hit
GTGGCAGCAATTTCATCTGTATCCTTTAAATTCAGCGATGTCTTGTAGAA14020.11374856698711526No Hit
TACTGGTGTGGGTTATAGAAGAAACATGAGAGATTTAGTTCAAAAGGAAG13900.11277497012274623No Hit
TTATCGCCATCCTTCTGAACTTAAGAAGAGCTCTAAAAAGCCAGTGTACA13820.1121259055465002No Hit
CCAGTACTAATGAAGTGGGCTCCAAAATACACCTGAATGTAGAACCATGT13810.11204477247446945No Hit
GGAAGGTGACGTGAAAGATCACTGTGCAGCAGCAATCTTGACTTCTGGAA13780.1118013732583772No Hit
TAATCTATATTGATCAGGTGAAGTAAAATGGAGTACGGTAGCAGACCTTT13720.11131457482619267No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC13610.1104221110338544No Hit
GCAGTCTGGGGCCCTTTGGCAGGCCCTGGTTGCCCTGAGGCGGGTGCTGC13520.10969191338557763No Hit
CAGCTGTGCCATTTGAGGGATGCTCAGGTCGGTTTGGCTCTGCAGCAGGT13330.10815038501699333No Hit
TAAGGAGAATGATTTGTAGTGGCCAAAATTGAATGCTATGCTTAGATGTA13180.10693338893653204No Hit
ATCACAATATCTCATTTTGCCCTCTTTGCAACCAGTGCACATTACTGGGT13120.10644659050434752No Hit
ACTTTCAAGTTTCCCCACCCTTTACACAGTTTCCCCGCGGAAAACGCCGC13050.10587865900013224No Hit
TAATCTATATTGATCAGGTGAAGTAAAATGGAGTTTGGGTATCTGCACTA12900.10466166291967095No Hit
CCAGATCCTTAACATTGGTCCGTATTTGAAGTCCCACTTTCTCATGGTTA12880.10449939677560946No Hit
AGGCCCTGCTGACCATACATAGAAGTTCATGGACACCTCGATAACTCAGA12770.10360693298327116No Hit
CAAAATCTAAAGCAGCAGACCTTTGTGCTTTTATCCCTGTAGAACCATGT12630.10247106997484064No Hit
CTTAAGAAATAACTGTGATTTGGCCTCCAAAATACACCTGAATGTAGAAC12510.1014974731104716No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)