FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361393
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC89592.479018686028783No Hit
TGAACTTATATATTTGACACAACCCAGCAACCCATTCAGCAGAATTAGAC83972.3235093098095425No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC78722.178238095369861No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCTGGTTCTACAAGCAGCTTC70621.9541053645200652No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC67301.8622386155791617No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA58701.6242705309732066No Hit
GGGGACGCTCTTGTATTATCTGTACACTGACACTGATTTGGAGTGAACTC57261.584424712155465No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT54701.513587700923925No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT54531.5088836806468304No Hit
TTCTGATGAGCCAAGTCTCTGTGGGAGGAGGGCAGTACACAGACCAAGTC52771.4601832354251465No Hit
AGACATCGCTGAATTTAAAGGATACAGATGAAATTGCTGCCACAGACCAA51931.4369398411147976No Hit
GGGAAAAGAACAGGCTTCACCTATCATCGTTTTCCAAGTTATTTTTGAGA51811.433619356213319No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC49131.3594618600803003No Hit
CCAAATCTGCTTTCTTGATAAAATCCTTTGAATGGCATGCAGACCAAGTC49001.3558646681036988No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT48641.3459032133992634No Hit
GTTGTTTCAGAACACCGAAAAGCAAACTAAGTATCAAGTGAGCAAGACCA46611.289731677149253No Hit
TTTTTAGGAAGTACATCTCAGAATCTTGATTCTGGAACAGATTTGTCTTT46591.2891782629990067No Hit
AGTGGAACTGTTATTATCCCTCAAATGGCACAGCTGAGACCAAGTCTCTG46121.276173030468216No Hit
AGTGGAACTGTTATTATCCCATCAAATGGCACAGCTGAGACCAAGTCTCT39891.1037845226664602No Hit
GACTACAGATGTGCCACCATACCAACTGCTCTGGAACATGTTAGACCAAG39301.087458805234191No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA37681.042632259064232No Hit
TCAAAGGGCTCCACTGGTTTTTCTTATCTCTTTCATTTGATTAGACCAAG35820.991164743091316No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA35740.9889510864903304No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC35710.9881209652649608No Hit
TTTCATATAGGCTGTGCAACAAGTACTTGGTCTCACCATCCAAAAGACCT34620.9579598940765316No Hit
GAAGTATTACAAATGGAAGATGATCTGGTGCACTTTTCTGAGGACCATAA34190.9460614898462338No Hit
GAAAGTGGTGCATTGATGAAGGCCTGATGTAGGTCTCAGACCAAGTCTCT33230.9194976106344063No Hit
GGGGTCAGAGGCAAGCAGATATCCGGTGTAGACCAAGTCTCTGCTACCGT33210.9189441964841598No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC31950.8840791050186363No Hit
AGCACCAAACGTAAAGCAGCAACAGAGGAGGGGGAGACCAAGTCTCTGCT30060.8317814678203507No Hit
TGGGGAAAAATATGCTTTGATCTACAGAAAAACATAGAAATCAGAGACCA29340.8118585584114801No Hit
GAAAGTGGTGCATTGATGGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC29040.803557346157784No Hit
TGGGGAAAAATATGCTTTGAGCTACAGAAAAACATAGAAATCAGAGACCA28280.7825276084484203No Hit
GGCTTCCACTTAATTCCAGTCTAAAAAGTTACCCAGAAGTGTAGACCAAG28130.7783770023215724No Hit
AGTTCCATTTAGGTTGCTGGTGGTGAAGAACATTCAGGCAAGACCAAGTC27810.7695223759176298No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGAAAAATGAAATACTTTG27450.7595609212131945No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAGTC27270.7545801938609769No Hit
TCTAAGCATAGCATTCAATTTTGGCCACTACAAATCATTCTCCTTAAGAC26320.7282930217242725No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC26250.72635607219841No Hit
GTGCACATTCAAAAAAGAAATTATCCAGATTTCCTTTCAGAACACTCAGA26080.7216520519213155No Hit
TCAAAGGGCTCCACAGTTTTTCTTATCTCTTTCATTTGATTAGACCAAGT26030.7202685165456996No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAGTCTAGC25580.7078166981651554No Hit
AGCCAAAATATACCTGGGAAATGATACGGTAGAGGAGCTTGTCCCGCTAG25290.6997921929865825No Hit
CCAAAATATGTCTGGATTGGAGAGAAATCTGTATTAACAGTCTGAGACCA24800.6862335463055454No Hit
GGGCAGTTGGCCACTTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTG24050.6654805156713052No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC23850.659946374168841No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA23690.6555190609668698No Hit
GGGCAGTTGGCCACTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTGC22480.6220375048769622No Hit
GGGGTCAGAGGAAGCAGATATCCGGTGTAGACCAAGTCTCTGCTACCGTA22290.6167800704496214No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC21770.6023913025432147No Hit
AGAAAACGGCATTTTGAGTGAGGCATAACTGCACCCTTAGACCAAGTCTC20990.5808081506836048No Hit
GCTTTCACTTCTCTGGCTGAACACACAGCTCAGAATACTAGTAGACCAAG20980.5805314436084816No Hit
GGTGCCAGTTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACCG19530.540408917715617No Hit
AACTGATCACCTTGAATCTGCTTGGGAGAAGGAATATTTGATGGTCAACC19480.5390253823400011No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA19050.5271269781097033No Hit
ATTATCCAGATGAATTTACAAAACTGTACATGTGCTCTTGAGTGAGACCA18660.5163354021798983No Hit
GGGATAAAAGCACAAAGGTCTGCTACCGTAACCTTCAGAGCTAGGGAAAA18580.5141217455789127No Hit
GTATAATGATGAGCATTTTGGCTACCGTACACATAGGTTAACATTTACCA18430.5099711394520647No Hit
TCTCCCCCACCCCTTCTACTCACTCAAAGACATCTGCAGACCAAGTCTCT18340.5074807757759559No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAGT18210.5038835837993542No Hit
TGGCTTATAAAATATTAATGTGCTTCTGTTTCAAAAGACCAAGTCTCTGC17790.4922618866441796No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAGTCT17470.4834072602402371No Hit
CAGCAATGTGGCTTTAAGTAGAACATATCATCTGGACCCTTTTCAGACCA15190.42031804711214665No Hit
TGTAGTTTCTAAGGGTCGAATTCTTTAAGTTCACTGGTATTTGAGACCAA15090.41755097636091454No Hit
GCCCTCTCAGAAACATCTGCTACCGTAACTTCGAGGGCTAAACCACAAGA14900.41229354193357365No Hit
AGTTCCATTTAGGTTGCTGGTGAAGAACATTCAGGCAAGACCAAGTCTCT14660.40565257213061684No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT13750.38047222829440525No Hit
CATCTCCATGAGTAGGAAGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC12570.3478207934298672No Hit
GTTGTTTCAGAACACCGAAAGCTCAAGAAGCATGTCATAGACCAAGTCTC12350.34173323777715675No Hit
TCTCCCCCACCCCCTCTACTCACTCAAAGACATCTGCAGACCAAGTCTCT12170.33675251042493903No Hit
CACTTAATGAGACCAACAGTAACACCTTTATCACTCACAAGACCAAGTCT12050.33343202552346063No Hit
CGCCCGGCCTTCATTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCA12000.3320484901478446No Hit
GGCTTCCACTTAATTCCAGTCTGAAAAGTTACCCAGAAGTGTAGACCAAG11770.3256842274200109No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC11730.3245773991195181No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAG11710.32402398496927165No Hit
CTGATGTTGAATTAACCAAAAATATTCCCTCTGCGTGTTCTCATAAGACC11570.3201500859175468No Hit
ACTCCAAAAAATACTAAGAGGCCCAATGTGACTACGGCAAGTATAGACCA11490.3179364293165612No Hit
TGCATTCTGAAAAAGCCAGAATTTACAGCTCTCCAGACCAAGTCTCTGCT11470.3173830151663148No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA11460.31710630809119156No Hit
AAGTCCTCCATTTCTGTATCCATGCCAGAACCCCTCAGATGTTAGACCAA11130.30797497461212586No Hit
CAGCAATGTGGCTTTAATTAGAACATATCATCTGGACCCTTTTCAGACCA11010.3046544897106474No Hit
GCCTGCTGAAAATGCCAGAACCCCTCAGATGTTAGACCAAGTCTCTGCTA11010.3046544897106474No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAGTCTAG10830.29967376235842974No Hit
CCAAATCTGCTTTCTTGATAAAATCCTCTGAATGGCATGCAGACCAAGTC10750.29746010575744414No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTAGTCTAGCAGGATC10720.2966299845320745No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA10390.28749865105300876No Hit
GCGTCTTCACCTGGAAGGCAGTCTGGCTGAGACCAAGTCTCTGCTACCGT9690.2681291557943845No Hit
TCAATGCAGAGGTTGAAGATGGTGTCTAGACCAAGTCTCTGCTACCGTAG9420.260658064766058No Hit
GCCTGCTGAAAATGACTGAACCCCTCAGATGTTAGACCAAGTCTCTGCTA9120.2523568525123619No Hit
CTACTAATGCCCACAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTCT8980.24848295346063703No Hit
TTTTAAGATGGTGTTTAAAAGAAGTAACCCAGAACTGTCAAATGACCAAG8830.24433234733378895No Hit
ACACAGATTCTGAAATGAGAGCTACCGTATGGATGATGATGTTGAAATAT8700.2407351553571873No Hit
GTTGTTTCAGAACACCGAAAAGCTCAAGAAGCATGTCATAGACCAAGTCT8580.23741467045570885No Hit
GCGTCTTCACCCGGAAGGCAGTCTGGCTGAGACCAAGTCTCTGCTACCGT8490.23492430677960005No Hit
CTAAATACGTTTCACAGGTAGAAAAATAAAACAATTGTGTCAACCAGAGA8300.22966687235225916No Hit
AAGTCCTCCATTTCTGTATCCATGGATTCTGAATGTGCTTAGACCAAGTC8080.22357931669954867No Hit
AGTTCCATTTAGGTTGCTGGTGGAGAACATTCAGGCAAGACCAAGTCTCT7770.21500139737072937No Hit
CTACTAATGCCCACAAAGAGATACTTTCCTGAGTGCCAGACCAAGTCTCT7550.20891384171801888No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTAGTC7370.20393311436580122No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTAGTCT7330.20282628606530842No Hit
AGTAAGGGTGGCATCAAAAATTGGTTTTCTTTGCAGACCAAGTCTCTGCT7220.1997825082389532No Hit
GCTTTCACTTCTCTGGCTGAGCACACAGCTCAGAATACTAGTAGACCAAG7210.19950580116383No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG7180.19867567993846033No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAGGTAGTGATATAAAGGTAA6930.19175800306038027No Hit
AACAATCGACAGGCTAGAAGTTGAGACCAAGTCTCTGCTACCGTAGTCTA6720.18594715448279298No Hit
GATGTGCCACCATACCCAGAGACCAAGTCTCTGCTACCGTAGTCTAGCAG6400.17709252807885045No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG6180.17100497242613996No Hit
CATCTCCATGAGTAGGATGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC6090.16851460875003113No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA6070.16796119459978473No Hit
AGTTGTGGTTACCTAGTTATTAGGGAAGTAGTAAAGAATGAGAGGGGAGA6040.1671310733744151No Hit
GCCACACCTCACGTTAGAATTTTCCAGTGAAGACCAAGTCTCTGCTACCG5930.16408729554805987No Hit
AGAAAACGGCATTTTGAGTGTTACGGTAGCACAGCAAGTGGAAAATCTGT5810.16076681064658144No Hit
ATTACAACCCATCTGAGTGGAGATATGGCTAATAGGCCCTAGACCAAGTC5680.15716961866997978No Hit
AGTTGTGGTTACCTAGTTATTATGGAAGTAGTAAAGAATGAGAGGGGAGA5600.15495596206899415No Hit
TGCTAATTGGGCAGATAGAGCTTCGTTCAGACCAAGTCTCTGCTACCGTA5540.15329571961825492No Hit
CTGAGGGAAGCAGCAATTCTCTGCTACCGTACCAAGAGGGGGAAGAGGGA5180.1433342649138196No Hit
CACTTAATGAGACCAACAGTGACACCTTTATCACTCACAAGACCAAGTCT5060.14001378001234113No Hit
AAGTCCTCCATTTCTGTACCCATGGATTCTGAATGTGCTTAGACCAAGTC5050.13973707293721793No Hit
GCAGAACATTTTGTTTCCTCACTTACGGTAGCAGCATCTTGAATCTCATA5010.13863024463672513No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT4980.1378001234113555No Hit
CCGGTACAAACCTTTCATTGTATTGTCGTTTTAGTTCAACCAGACCAAGT4930.1364165880357395No Hit
AGTAATCGCATGGTGGTGAAGAACATTCAGGCAAGACCAAGTCTCTGCTA4870.13475634558500027No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG4860.13447963850987707No Hit
GAGTTTGATGCACAACTTCAAGGCCACTGACTTATGGTATCAAATAATAA4660.12894549700741298No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAAAAATGAAATACTTTGAG4650.12866878993228978No Hit
ATTATCCAGATGAATTTACAAAACTGTACCTGTGCTCTTGAGTGAGACCA4630.12811537578204338No Hit
TGATAAATGCACCTGTTTGCTGTCACATTCAGAGACCAAGTCTCTGCTAC4580.12673184040642735No Hit
AAGTCCTCCATTTCTGTATCCATGGAGGAAGTAGGCAGCTGAGACCAAGT4550.12590171918105775No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT4420.1223045272044561No Hit
ACATGTACATAGGGTTTATATATCATCAGCTATAAGTATCTTCTCTTGTC4090.11317319372539036No Hit
AAGTCCTCCATTTCTGTATCCATGCGAGACCAAGTCTCTGCTACCGTAGT4020.11123624419952793No Hit
CCAGACTCTCCAGAACCAAAGGTGCTGACTACTACCGCTAGACCAAGTCT3980.11012941589903513No Hit
AACTGATCACCTTGAATCTGCTTGGAGAAGGAATATTTGATGGTCAACCA3890.10763905222292629No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG3810.10542539562194066No Hit
ATACCACTGACGGCCAGGTAGAGATTTTATTTTGTTTTTCAGAGACCAAG3730.10321173902095504No Hit
ATATTTAGTGAATGTGATTGATGGTACTTTACACTGACGACATGGTTCTA3730.10321173902095504No Hit
CCAGCTCACAAGAGAAGAAACAAAGGCAACCCTCCTAAACCACTGAAAAA3680.10182820364533901No Hit
GCAATAAAACTAGTAGTGCAGATACCCACAAACTGTAAATGAAGACCAAG3670.10155149657021581No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)