FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008103035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008103035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361393
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG84152.3284900371617603No Hit
AATTCTGCTGAATGGGTTGCTGGGTTGTGTCAAATATATAAGTTCATGTA83082.2988823801235774No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG78072.1602521354868522No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA66981.8533839891752193No Hit
AGAGACATCTTAAAGAGGGAAGCTGCTTGTAGAACCAGCCTTTCTTCATC66191.831524130240486No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT57491.5907889748832986No Hit
GTGAAAGAGTTCACTCCAAATCAGTGTCAGTGTACAGATAATACAAGAGC56271.5570307117182678No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC54181.4991989330175184No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT54011.494494912740424No Hit
GTGTACTGCCCTCCTCCCACAGAGACTTGGCTCATCAGAATGTAGAACCA52471.4518820231714504No Hit
GTGGCAGCAATTTCATCTGTATCCTTTAAATTCAGCGATGTCTTGTAGAA51681.4300221642367172No Hit
CAAAAATAACTTGGAAAACGATGATAGGTGAAGCCTGTTCTTTTCCCTGT51591.4275318005606086No Hit
GCATGCCATTCAAAGGATTTTATCAAGAAAGCAGATTTGGTGTAGAACCA49011.356141375178822No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG48941.3542044256529595No Hit
TTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT48221.3342815162440889No Hit
GGGATTCCATGATTCGATGTTCACATCGTTCTAAATGTTTTATCATTTCT46271.280323636595064No Hit
TGCTCACTTGATACTTAGTTTGCTTTTCGGTGTTCTGAAACAACTGTAGA46041.2739593738672303No Hit
CAGCTGTGCCATTTGAGGGATAATAACAGTTCCACTTGTAGAACCATGTC45781.266764989914027No Hit
CAGCTGTGCCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGT39661.0974202599386262No Hit
AACATGTTCCAGAGCAGTTGGTATGGTGGCACATCTGTAGTCTGTAGAAC39041.0802644212809878No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT37131.0274133699324557No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG35740.9889510864903304No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT35490.9820334096122504No Hit
AATCAAATGAAAGAGATAAGAAAAACCAGTGGAGCCCTTTGATGTAGAAC35400.9795430459361416No Hit
TATGGTCCTCAGAAAAGTGCACCAGATCATCTTCCATTTGTAATACTTCT33820.9358233280666752No Hit
GAGACCTACATCAGGCCTTCATCAATGCACCACTTTCTGTAGAACCATGT33210.9189441964841598No Hit
ACACCGGATATCTGCTTGCCTCTGACCCCTGTAGAACCATGTCGTCAGTG33080.9153470045075582No Hit
ACCTGGATATAGGTCTTTTGGATGGTGAGACCAAGTACTTGTTGCACAGC32510.8995747012255356No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA31790.8796517918166649No Hit
CCCCCTCCTCTGTTGCTGCTTTACGTTTGGTGCTTGTAGAACCATGTCGT29730.8226501343412849No Hit
CTGATTTCTATGTTTTTCTGTAGATCAAAGCATATTTTTCCCCATGTAGA29050.8038340532329071No Hit
GAGACCTACATCAGGCCTTCCATCAATGCACCACTTTCTGTAGAACCATG28740.7952561339040878No Hit
CTGATTTCTATGTTTTTCTGTAGCTCAAAGCATATTTTTCCCCATGTAGA27970.7739496891196012No Hit
ACACTTCTGGGTAACTTTTTAGACTGGAATTAAGTGGAAGCCTGTAGAAC27900.7720127395937386No Hit
TGCCTGAATGTTCTTCACCACCAGCAACCTAAATGGAACTTGTAGAACCA27460.7598376282883177No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA27330.756240436311716No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTTCTAAGAACACAGAGGAGAATTT27040.7482159311331431No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG26250.72635607219841No Hit
TAAGGAGAATGATTTGTAGTGGCCAAAATTGAATGCTATGCTTAGATGTA25950.718054859944714No Hit
GAGTGTTCTGAAAGGAAATCTGGATAATTTCTTTTTTGAATGTGCACTGT25890.7163946174939747No Hit
AATCAAATGAAAGAGATAAGAAAAACTGTGGAGCCCTTTGATGTAGAACC25820.7144576679681123No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC25380.7022825566626912No Hit
AGCGGGACAAGCTCCTCTACCGTATCATTTCCCAGGTATATTTTGGCTTG25110.6948114656343648No Hit
CAGACTGTTAATACAGATTTCTCTCCAATCCAGACATATTTTGGTGTAGA24490.6776556269767262No Hit
CTGCACCCAGCCATAAGTAAAAGTGGCCAACTGCCCTGTAGAACCATGTC23930.6621600307698268No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT23460.6491547982390362No Hit
GAGTCCTCCTTCTGTGAGCAAACTGGCACCTGTAGAACCATGTCGTCAGT23160.64085358598534No Hit
CTGCACCCAGCCATAAGTAAAGTGGCCAACTGCCCTGTAGAACCATGTCG22290.6167800704496214No Hit
ACACCGGATATCTGCTTCCTCTGACCCCTGTAGAACCATGTCGTCAGTGT22170.6134595855481428No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG21950.6073720298954324No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG21780.6026680096183379No Hit
ACTAGTATTCTGAGCTGTGTGTTCAGCCAGAGAAGTGAAAGCTGTAGAAC20880.5777643728572496No Hit
AAGGGTGCAGTTATGCCTCACTCAAAATGCCGTTTTCTTGTAGAACCATG20790.5752740091811408No Hit
GGTTGACCATCAAATATTCCTTCTCCCAAGCAGATTCAAGGTGATCAGTT19380.536258311588769No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT18910.5232530790579784No Hit
CACTCAAGAGCACATGTACAGTTTTGTAAATTCATCTGGATAATTGTAGA18550.5132916243535431No Hit
GCAGATGTCTTTGAGTGAGTAGAAGGGGTGGGGGAGATGTAGAACCATGT18430.5099711394520647No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG18270.5055438262500934No Hit
TTTGAAACAGAAGCACATTAATATTTTATAAGCCATGTAGAACCATGTCG17610.4872811592919619No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT17360.4803634824138818No Hit
AGGGGTGGTAAATGTTAACCTATGTGTACGGTAGCCAAAATGCTCATCAT17190.4756594621367874No Hit
ACTTTTTCCCTAGCTCTGAAGGTTACGGTAGCAGACCTTTGTGCTTTTAT16680.46154740130550403No Hit
GAAAAGGGTCCAGATGATATGTTCTACTTAAAGCCACATTGCTGTGTAGA15130.41865780466140734No Hit
CAAATACCAGTGAACTTAAAGAATTCGACCCTTAGAAACTACATGTAGAA15030.41589073391017534No Hit
TGCCTGAATGTTCTTCACCAGCAACCTAAATGGAACTTGTAGAACCATGT14710.40703610750623287No Hit
TGTGGTTTAGCCCTCGAAGTTACGGTAGCAGATGTTTCTGAGAGGGCTGT14700.40675940043110964No Hit
GCAGATGTCTTTGAGTGAGTAGAGGGGGTGGGGGAGATGTAGAACCATGT13580.37576820801731076No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT13550.3749380867919412No Hit
GGAGAGCTGTAAATTCTGGCTTTTTCAGAATGCATGTAGAACCATGTCGT12750.3528015207820849No Hit
GAGACCTACATCAGGCCTTCTTCCTACTCATGGAGATGTGTAGAACCATG12590.3483742075801136No Hit
ATGACATGCTTCTTGAGCTTTCGGTGTTCTGAAACAACTGTAGAACCATG12310.3406264094766639No Hit
TGTGAGTGATAAAGGTGTTACTGTTGGTCTCATTAAGTGTGTAGAACCAT11910.32955812647173577No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGAAGGCCGGGCGTGTAGA11880.32872800524636614No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG11770.3256842274200109No Hit
ACACTTCTGGGTAACTTTTCAGACTGGAATTAAGTGGAAGCCTGTAGAAC11690.32347057081902525No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA11630.32181032836828605No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA11470.3173830151663148No Hit
TATGAGAACACGCAGAGGGAATATTTTTGGTTAATTCAACATCAGTGTAG11440.31655289394094516No Hit
ATACTTGCCGTAGTCACATTGGGCCTCTTAGTATTTTTTGGAGTTGTAGA11270.3118488736638507No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT11050.3057613180111402No Hit
AACATCTGAGGGGTTCTGGCATGGATACAGAAATGGAGGACTTTGTAGAA11010.3046544897106474No Hit
GAAAAGGGTCCAGATGATATGTTCTAATTAAAGCCACATTGCTGTGTAGA10980.30382436848527783No Hit
AACATCTGAGGGGTTCTGGCATTTTCAGCAGGCTGTAGAACCATGTCGTC10970.3035476614101546No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT10890.30133400480916894No Hit
GCATGCCATTCAGAGGATTTTATCAAGAAAGCAGATTTGGTGTAGAACCA10730.29690669160719774No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT10440.2888821864286248No Hit
CAGCCAGACTGCCTTCCAGGTGAAGACGCTGTAGAACCATGTCGTCAGTG9610.2659154991933989No Hit
GGCACTCAGGAAAGTATCTCGTTGTGGGCATTAGTAGTGTAGAACCATGT9010.24931307468600666No Hit
AACATCTGAGGGGTTCAGTCATTTTCAGCAGGCTGTAGAACCATGTCGTC9000.24903636761088344No Hit
TGGTCATTTGACAGTTCTGGGTTACTTCTTTTAAACACCATCTTAAAATG8730.24156527658255694No Hit
ATGACATGCTTCTTGAGCTTTTCGGTGTTCTGAAACAACTGTAGAACCAT8540.23630784215521605No Hit
CAGCCAGACTGCCTTCCGGGTGAAGACGCTGTAGAACCATGTCGTCAGTG8500.23520101385472325No Hit
GGAACATATTTCAACATCATCATCCATACGGTAGCTCTCATTTCAGAATC8450.23381747847910722No Hit
CTGGTTGACACAATTGTTTTATTTTTCTACCTGTGAAACGTATTTAGTGT8230.22772992282639676No Hit
AAGCACATTCAGAATCCATGGATACAGAAATGGAGGACTTTGTAGAACCA7980.22081224594831667No Hit
TGCCTGAATGTTCTCCACCAGCAACCTAAATGGAACTTGTAGAACCATGT7690.21278774076974372No Hit
GCAAAGAAAACCAATTTTTGATGCCACCCTTACTTGTAGAACCATGTCGT7560.20919054879314208No Hit
GGCACTCAGGAAAGTATCTCTTTGTGGGCATTAGTAGTGTAGAACCATGT7530.20836042756777248No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA7350.20337970021555482No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT7290.2017194577648156No Hit
ACTAGTATTCTGAGCTGTGTGCTCAGCCAGAGAAGTGAAAGCTGTAGAAC7230.2000592153140764No Hit
CAACTTCTAGCCTGTCGATTGTTTGTAGAACCATGTCGTCAGTGTAGATC6780.18760739693353218No Hit
AACATTTTACTTACCTTTATATCACTACCTGTACGGTAGCAGTGGGATCA6710.18567044740766978No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG6660.18428691203205375No Hit
CTGGGTATGGTGGCACATCTGTAGAACCATGTCGTCAGTGTAGATCTCGG6380.17653911392860405No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT6130.16962143705052396No Hit
CCCCTCTCATTCTTTACTACTTCCCTAATAACTAGGTAACCACAACTTGT6040.1671310733744151No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT6020.1665776592241687No Hit
GAGACCTACATCAGGCCTTCATCCTACTCATGGAGATGTGTAGAACCATG5980.16547083092367587No Hit
TCACTGGAAAATTCTAACGTGAGGTGTGGCTGTAGAACCATGTCGTCAGT5930.16408729554805987No Hit
ACAGATTTTCCACTTGCTGTGCTACCGTAACACTCAAAATGCCGTTTTCT5790.160213396496335No Hit
GAACGAAGCTCTATCTGCCCAATTAGCATGTAGAACCATGTCGTCAGTGT5700.15772303282022618No Hit
CTGAATGTGACAGCAAACAGGTGCATTTATCATGTAGAACCATGTCGTCA5660.15661620451973335No Hit
CCCCTCTCATTCTTTACTACTTCCATAATAACTAGGTAACCACAACTTGT5620.15550937621924055No Hit
AGGGCCTATTAGCCATATCTCCACTCAGATGGGTTGTAATTGTAGAACCA5560.15384913376850134No Hit
ATGAAAACACAACATGAATATAAACATCAATCACATTCACTAAATATTGT5280.14610133566505162No Hit
TGTGAGTGATAAAGGTGTCACTGTTGGTCTCATTAAGTGTGTAGAACCAT5070.14029048708746433No Hit
AAGCACATTCAGAATCCATGGGTACAGAAATGGAGGACTTTGTAGAACCA5040.1394603658620947No Hit
TCCCTCTTCCCCCTCTTGGTACGGTAGCAGAGAATTGCTGCTTCCCTCAG5020.1389069517118483No Hit
GTATGAGATTCAAGATGCTGCTACCGTAAGTGAGGAAACAAAATGTTCTG4960.1372467092611091No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT4890.13530975973524667No Hit
GGTTGAACTAAAACGACAATACAATGAAAGGTTTGTACCGGTGTAGAACC4870.13475634558500027No Hit
TGCCTGAATGTTCTTCACCACCATGCGATTACTTGTAGAACCATGTCGTC4830.13364951728450744No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTTAAGAACACAGAGGAGAATTTAT4790.1325426889840146No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC4770.1319892748337682No Hit
CACTCAAGAGCACAGGTACAGTTTTGTAAATTCATCTGGATAATTGTAGA4630.12811537578204338No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGGCCTTGAAGTTGTGC4580.12673184040642735No Hit
CAGCTGCCTACTTCCTCCATGGATACAGAAATGGAGGACTTTGTAGAACC4550.12590171918105775No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT4430.1225812342795793No Hit
CCATGGACAAGAGAAGATACTTATAGCTGATGATATATAAACCCTATGTA4060.11234307250002076No Hit
CGCATGGATACAGAAATGGAGGACTTTGTAGAACCATGTCGTCAGTGTAG3970.1098527088239119No Hit
AGCGGTAGTAGTCAGCACCTTTGGTTCTGGAGAGTCTGGTGTAGAACCAT3940.1090225875985423No Hit
GGTTGACCATCAAATATTCCTTCTCCAAGCAGATTCAAGGTGATCAGTTT3860.10680893099755669No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT3800.10514868854681746No Hit
CTGAAAAACAAAATAAAATCTCTACCTGGCCGTCAGTGGTATTGTAGAAC3680.10182820364533901No Hit
TCATTTACAGTTTGTGGGTATCTGCACTACTAGTTTTATTGCTGTAGAAC3630.100444668269723No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)